Summary of Study ST000779
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000565. The data can be accessed directly via it's Project DOI: 10.21228/M8N38V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000779 |
| Study Title | Lipidomics pilot project for mice exposure to bisphenol A and high fat diets |
| Study Type | MS analysis |
| Study Summary | Wild type (a/a) agouti mouse dams were randomized to one of three diets (control, Mediterranean, western) two weeks prior to pairing with an agouti (Avy/a) sire. Diet exposure continued through pregnancy and lactation. All pups were weaned onto the control diet and then followed for metabolic phenotyping measures to 10 months of age. Comprehensive phenotyping (body composition, CLAMS, blood draw) was completed at 2, 4, and 8 months, with an OGTT at 8 months. Weekly weights were also recorded. The study examines whether prenatal dietary exposure to high fat diets (HFD) and bisphenol a (BPA, those groups will not be tested in this pilot) impacts metabolic programming in offspring as measured by hepatic steatosis, serum hormone levels, and epigenetic changes in hepatic lipid metabolism genes. Shotgun lipidomics will add insight into the potentially changing lipid composition of membranes in offspring following different prenatal diet exposures as a means of assessing risk of steatosis and metabolic changes. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 8 |
| Total Subjects | 3 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000565 |
| Project DOI: | doi: 10.21228/M8N38V |
| Project Title: | Mice exposure to bisphenol A and high fat diets |
| Project Type: | MS analysis |
| Project Summary: | Prenatal exposure to Bisphenol A (BPA) and high fat diets (HFDs) are studied to determine impact on metabolic syndrome and non-alcoholic fatty liver disease outcomes in mouse pups from birth through 10 months |
| Institute: | University of Michigan |
| Department: | Environmental Health Sciences |
| Laboratory: | Dolinoy Lab |
| Last Name: | Dolinoy |
| First Name: | Dana |
| Address: | Ann Arbor, MI |
| Email: | ddolinoy@umich.edu |
| Phone: | 734-647-3155 |
Subject:
| Subject ID: | SU000802 |
| Subject Type: | MOUSE |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Age (months) | Diet |
|---|---|---|---|
| SA042389 | S00016769 | 10.1 | Mediterranean |
| SA042390 | S00016772 | 10.1 | Mediterranean |
| SA042391 | S00016767 | 10.3 | Western |
| SA042392 | S00016764 | 10.4 | Control |
| SA042393 | S00016765 | 10.4 | Control |
| SA042394 | S00016766 | 10.4 | Mediterranean |
| SA042395 | S00016768 | 10.4 | Western |
| SA042396 | S00016770 | 10.5 | Control |
| SA042397 | S00016771 | 10.5 | Western |
| Showing results 1 to 9 of 9 |
Collection:
| Collection ID: | CO000796 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000816 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000809 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN001232 | AN001233 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area normalized | peak area normalized |
Chromatography:
| Chromatography ID: | CH000863 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001127 |
| Analysis ID: | AN001232 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS001128 |
| Analysis ID: | AN001233 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
