Summary of study ST000779

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000565. The data can be accessed directly via it's Project DOI: 10.21228/M8N38V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000779
Study TitleLipidomics pilot project for mice exposure to bisphenol A and high fat diets
Study TypeMS analysis
Study SummaryWild type (a/a) agouti mouse dams were randomized to one of three diets (control, Mediterranean, western) two weeks prior to pairing with an agouti (Avy/a) sire. Diet exposure continued through pregnancy and lactation. All pups were weaned onto the control diet and then followed for metabolic phenotyping measures to 10 months of age. Comprehensive phenotyping (body composition, CLAMS, blood draw) was completed at 2, 4, and 8 months, with an OGTT at 8 months. Weekly weights were also recorded. The study examines whether prenatal dietary exposure to high fat diets (HFD) and bisphenol a (BPA, those groups will not be tested in this pilot) impacts metabolic programming in offspring as measured by hepatic steatosis, serum hormone levels, and epigenetic changes in hepatic lipid metabolism genes. Shotgun lipidomics will add insight into the potentially changing lipid composition of membranes in offspring following different prenatal diet exposures as a means of assessing risk of steatosis and metabolic changes.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups8
Total Subjects3
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8N38V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000565
Project DOI:doi: 10.21228/M8N38V
Project Title:Mice exposure to bisphenol A and high fat diets
Project Type:MS analysis
Project Summary:Prenatal exposure to Bisphenol A (BPA) and high fat diets (HFDs) are studied to determine impact on metabolic syndrome and non-alcoholic fatty liver disease outcomes in mouse pups from birth through 10 months
Institute:University of Michigan
Department:Environmental Health Sciences
Laboratory:Dolinoy Lab
Last Name:Dolinoy
First Name:Dana
Address:Ann Arbor, MI
Email:ddolinoy@umich.edu
Phone:734-647-3155

Subject:

Subject ID:SU000802
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Age (months) Diet
SA042389S0001676910.1 Mediterranean
SA042390S0001677210.1 Mediterranean
SA042391S0001676710.3 Western
SA042392S0001676410.4 Control
SA042393S0001676510.4 Control
SA042394S0001676610.4 Mediterranean
SA042395S0001676810.4 Western
SA042396S0001677010.5 Control
SA042397S0001677110.5 Western
Showing results 1 to 9 of 9

Collection:

Collection ID:CO000796
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000816
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000809
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001232 AN001233
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um) Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000863
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001127
Analysis ID:AN001232
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001128
Analysis ID:AN001233
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
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