Summary of study ST000833

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000594. The data can be accessed directly via it's Project DOI: 10.21228/M8WD50 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000833
Study TitleInfluence of murine norovirus on cell metabolism
Study TypeMS analysis
Study Summary6.5 X 10^6 cells were plated in 10 cm Tissue Culture dishes and allowed to recover overnight at 37 degrees and 5% CO2. In the am, supernatant was removed, and 12 mL of medium containing either Murine Norovirus-1 (MNV) at an MOI=5 or medium containing a v/v match of mock lysate was added to the cells. Plates were rocked on ice for 1 hour, then cells were washed 3X with cold DPBS++, and plain medium as added to cells. Plates were then incubated for 7.5 hours at 37 degrees and 5% CO2. Cells were washed with 12 mL of 150 mM Ammonium Acetate, swirled 8 times, and immediately quenched with liquid nitrogen. Cells were then frozen at -80 degrees.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups2
Total Subjects1
Study CommentsBIOHAZARD - MURINE NOROVIRUS - SHOULD BE ENTIRELY NON-INFECTIOUS DUE TO THE LIQUID NITROGEN, BUT PLEASE HANDLE AND DISPOSE OF IN BIOHAZARD WASTE. IMPORTANT NOTE: I request that the metabolites of the TCA+ assay that are to be assessed quantitatively please be sure to include ATP, ADP, AMP, NAD, NADH, NADP, NADPH, E4P, S7P, 6PG, G3P H6P and Lactate. Also, I would request that both reduced and oxidized Glutathione as well as Fructose 1,6-bisphosphate be included in the non-quantitative data.
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailGC/LC-MS
Release Date2018-08-27
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8WD50
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000594
Project DOI:doi: 10.21228/M8WD50
Project Title:Influence of murine norovirus on cell metabolism
Project Type:MS analysis
Project Summary:C6 effects in RAW
Institute:University of Michigan
Department:Microbiology & Immunology
Laboratory:Passalacqua Lab
Last Name:Passalacqua
First Name:Karla
Address:Ann Arbor, MI
Email:kpassal@umich.edu
Phone:734-763-5639

Subject:

Subject ID:SU001180
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Medium
SA077807S00023724MNV infected
SA077808S00023723MNV infected
SA077809S00023722MNV infected
SA077810S00023720mock lysate
SA077811S00023721mock lysate
SA077812S00023719mock lysate
Showing results 1 to 6 of 6

Collection:

Collection ID:CO001174
Collection Summary:-
Sample Type:Cultured cells

Treatment:

Treatment ID:TR001195
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP001188
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001841 AN001842 AN001843
Analysis type MS MS MS
Chromatography type HILIC GC HILIC
Chromatography system Agilent Agilent GC_7890N Agilent
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Agilent HP5-MS (15m × 0.25mm, 0.25 um) Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI EI ESI
MS instrument type QTOF Single quadrupole QTOF
MS instrument name Agilent 6520B QTOF Agilent MS_5975 Agilent 6520B QTOF
Ion Mode POSITIVE POSITIVE POSITIVE
Units uM pmol/ug protein counts

Chromatography:

Chromatography ID:CH001318
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH001319
Instrument Name:Agilent GC_7890N
Column Name:Agilent HP5-MS (15m × 0.25mm, 0.25 um)
Chromatography Type:GC
  
Chromatography ID:CH001320
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS001702
Analysis ID:AN001841
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:none
Ion Mode:POSITIVE
  
MS ID:MS001703
Analysis ID:AN001842
Instrument Name:Agilent MS_5975
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:none
Ion Mode:POSITIVE
  
MS ID:MS001704
Analysis ID:AN001843
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:none
Ion Mode:POSITIVE
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