Summary of study ST000837

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000597. The data can be accessed directly via it's Project DOI: 10.21228/M8H678 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000837
Study TitleHepatocyte Differentiation Metabolism
Study TypeMS analysis
Study SummaryIn the first group of samples, we are looking into the different metabolites and their concentrations within our human embryonic stem cells over the course of differentiation. Our goal is to study how the metabolism of stem cells are changing as they undergo this conversion to become hepatocyte-like cells. In the second group of samples labeled HyCell, we would like to understand the concentrations of metabolites within our CHO cells in order to update the parameters for our metabolic model. Included are two samples from different days of a suspension batch culture which may have different lactate production qualities.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups9
Total Subjects5
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8H678
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000597
Project DOI:doi: 10.21228/M8H678
Project Title:Hepatocyte Metabolism
Project Type:MS analysis
Project Summary:Differentiating Stem Cells towards Hepatocytes
Institute:University of Minnesota
Department:Chemical Engineering
Laboratory:Wei-Shou Hu Lab
Last Name:Hu
First Name:Wei-Shou
Address:St. Paul, Minnesota
Email:wshu@umn.edu
Phone:612-625-0546

Subject:

Subject ID:SU000864
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell Line DAYS Species
SA046632S00028164H9 10 Homo sapiens
SA046633S00028163H9 2 Homo sapiens
SA046631S00028162H9 - Homo sapiens
SA046634S00028165HyCell 3 Rodentia
SA046635S00028166HyCell 4 Rodentia
Showing results 1 to 5 of 5

Collection:

Collection ID:CO000858
Collection Summary:-
Sample Type:Stem cells

Treatment:

Treatment ID:TR000878
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000871
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001340 AN001341
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent Agilent
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6520B QTOF Agilent 6520B QTOF
Ion Mode NEGATIVE NEGATIVE
Units pmol/ug protein counts

Chromatography:

Chromatography ID:CH000936
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH000937
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS001232
Analysis ID:AN001340
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001233
Analysis ID:AN001341
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
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