Summary of study ST000839

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000542. The data can be accessed directly via it's Project DOI: 10.21228/M8M96V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000839
Study TitleEffect of cooling on muscle tissue metabolism (part II)
Study TypeMS analysis
Study SummaryThermal modalities are commonly used in sports medicine to affect tissue healing. Cold therapy is commonly used modalities, but the metabolic changes to muscle after cooling are not known. The objective of this study is to look at the effect of cooling on muscle metabolites and gene expression. There are a total of 8 subjects in the study. Each subject had an ice cup cryotherapy treatment ("cool" samples) to one leg for 15 minutes, and the other leg served as the control ("cntrl" samples). Two hours after the application of cryotherapy, a biopsy was taken from each thigh muscle. Muscle was minced with scissors and quickly snap frozen in liquid nitrogen.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups10
Total Subjects8
Study CommentsWe would like to run the TCA Plus Analysis on each of the samples. For the statistical analysis, we would normalize the cool side to the ctrl side, and look at the relative change in metabolite abundance as a result of the cooling procedure. only used in sports medicine to affect tissue healing.
Raw Data AvailableYes
Raw Data File Type(s).D
Analysis Type DetailLC-MS
Release Date2018-08-27
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8M96V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000542
Project DOI:doi: 10.21228/M8M96V
Project Title:Thermal Modalities Study
Project Type:MS analysis
Project Summary:This study is looking at the effect of cooling on muscle tissue metabolism
Institute:University of Michigan
Department:Orthopaedic Surgery
Laboratory:Mendias Lab
Last Name:Mendias
First Name:Christopher
Address:Ann Arbor, MI
Email:cmendias@umich.edu
Phone:734-764-3250

Subject:

Subject ID:SU000866
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Temperature
SA046648S00023215Control
SA046649S00023225Control
SA046650S00023229Control
SA046651S00023221Control
SA046652S00023227Control
SA046653S00023223Control
SA046654S00023217Control
SA046655S00023219Control
SA046656S00023230Cooled
SA046657S00023216Cooled
SA046658S00023228Cooled
SA046659S00023226Cooled
SA046660S00023220Cooled
SA046661S00023224Cooled
SA046662S00023222Cooled
SA046663S00023218Cooled
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000860
Collection Summary:-
Sample Type:Muscle

Treatment:

Treatment ID:TR000880
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000873
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001345 AN001346
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent Agilent
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6520B QTOF Agilent 6520B QTOF
Ion Mode NEGATIVE NEGATIVE
Units nmol/mg counts

Chromatography:

Chromatography ID:CH000941
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH000942
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS001237
Analysis ID:AN001345
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS001238
Analysis ID:AN001346
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
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