Summary of Study ST000883

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000612. The data can be accessed directly via it's Project DOI: 10.21228/M8FM7S This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000883
Study TitleBreathprinting Reveals Malaria-Associated Biomarkers and Mosquito Attractants
Study SummaryCurrent evidence suggests that malaria infection could alter patient breath metabolites, a phenomenon that could be exploited to create a breath-based diagnostic test. Indications include the preferential attraction of the Anopheles mosquito vector upon infection and a distinct breath profile with the progression of experimental, sub-microscopic malaria. However, these observations have yet to be extended to the clinic. To investigate whether natural human malaria infection leads to a characteristic breath profile, we performed a field study in Malawi. Breath volatiles from pediatric patients with and without uncomplicated falciparum malaria were analyzed by thermal desorption-gas chromatography/mass spectrometry. Using an unbiased, correlation-based analysis, we find that children with malaria have a distinct shift in overall breath composition. Leveraging these differences, highly accurate classification of infection status was achieved with a suite of six compounds. In addition, we find that malaria-infected children have significantly higher breath levels of two mosquito-attractant terpenes, α-pinene and 3-carene. Thus, our work attests to the viability of breath analysis for malaria diagnosis, identifies candidate compounds for follow-up studies, and identifies biologically plausible chemical mediators for increased mosquito attraction to malaria-infected patients.
Institute
Washington University in St. Louis
DepartmentSchool of Medicine
Last NameSchaber
First NameChad
Address4938 Parkview Place, MPRB/FLoor 6, Entry 5, St. Louis, MO, 63110, USA
Emailchadschaber@wustl.edu
Phone3142862040
Submit Date2017-10-08
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2018-02-05
Release Version1
Chad Schaber Chad Schaber
https://dx.doi.org/10.21228/M8FM7S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000612
Project DOI:doi: 10.21228/M8FM7S
Project Title:Breathprinting Reveals Malaria-Associated Biomarkers and Mosquito Attractants
Project Summary:Current evidence suggests that malaria infection could alter patient breath metabolites, a phenomenon that could be exploited to create a breath-based diagnostic test. Indications include the preferential attraction of the Anopheles mosquito vector upon infection and a distinct breath profile with the progression of experimental, sub-microscopic malaria. However, these observations have yet to be extended to the clinic. To investigate whether natural human malaria infection leads to a characteristic breath profile, we performed a field study in Malawi. Breath volatiles from pediatric patients with and without uncomplicated falciparum malaria were analyzed by thermal desorption-gas chromatography/mass spectrometry. Using an unbiased, correlation-based analysis, we find that children with malaria have a distinct shift in overall breath composition. Leveraging these differences, highly accurate classification of infection status was achieved with a suite of six compounds. In addition, we find that malaria-infected children have significantly higher breath levels of two mosquito-attractant terpenes, α-pinene and 3-carene. Thus, our work attests to the viability of breath analysis for malaria diagnosis, identifies candidate compounds for follow-up studies, and identifies biologically plausible chemical mediators for increased mosquito attraction to malaria-infected patients.
Institute:Washington University in St. Louis
Department: School of Medicine
Last Name:Schaber
First Name:Chad
Address:4938 Parkview Place, MPRB/FLoor 6, Entry 5, St. Louis, MO, 63110, USA
Email:chadschaber@wustl.edu
Phone:3142862040

Subject:

Subject ID:SU000917
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Malaria Infection Status
SA051168Patient 14Negative
SA051169Patient 13Negative
SA051170Patient 12Negative
SA051171Patient 15Negative
SA051172Patient 16Negative
SA051173Patient 1Negative
SA051174Patient 17Negative
SA051175Patient 11Negative
SA051176Patient 18Negative
SA051177Patient 4Negative
SA051178Patient 3Negative
SA051179Patient 10Negative
SA051180Patient 5Negative
SA051181Patient 2Negative
SA051182Patient 9Negative
SA051183Patient 6Negative
SA051184Patient 8Negative
SA051185Patient 7Negative
SA051186Patient 31Positive
SA051187Patient 29Positive
SA051188Patient 32Positive
SA051189Patient 30Positive
SA051190Patient 34Positive
SA051191Patient 28Positive
SA051192Patient 35Positive
SA051193Patient 33Positive
SA051194Patient 23Positive
SA051195Patient 21Positive
SA051196Patient 20Positive
SA051197Patient 19Positive
SA051198Patient 22Positive
SA051199Patient 24Positive
SA051200Patient 26Positive
SA051201Patient 25Positive
SA051202Patient 27Positive
Showing results 1 to 35 of 35

Collection:

Collection ID:CO000911
Collection Summary:Breath collection was performed as previously reported with alterations detailed here (see PMID: 25810441). In brief, ≥ 1 L of exhaled breath was collected in a 3 L SamplePro Flexfilm sample bag (SKC Inc.). Using a set flow pump (ACTI-VOC, Markes International), exactly 1 L of breath was pumped through an inert stainless-steel sorbent tube with a triple bed sorbent composed of Tenax 60/80/ Carbograph 1 60/80/ Carboxen 1003 40/60 (Camsco). Sorbent tubes were stored at -20°C prior to shipment on black ice for later mass spectrometric analysis.
Sample Type:Breath

Treatment:

Treatment ID:TR000931
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP000924
Sampleprep Summary:N/A

Combined analysis:

Analysis ID AN001440
Analysis type MS
Chromatography type GC
Chromatography system Leco Pegasus 4D GC
Column Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus 4D GCxGC TOF
Ion Mode POSITIVE
Units raw abundance

Chromatography:

Chromatography ID:CH001011
Chromatography Summary:All samples were run with a TurboMatrix 650 ATD (Perkin Elmer) connected to a Leco Pegasus 4D GCxGC-TOFMS system. Before analysis, sorbent tubes were brought to room temperature and purged for 5 min with BiP N2 (Airgas) at 60 mL/min. A gaseous standard mixture (20.1 ng fluorobenzene, 18.6 ng toluene-D8, 21.7 ng bromofluorobenzene, 20.3 ng 1,2-dichlorobenzene-D4) was added to each tube by the TurboMatrix 650 immediately prior to analysis. Tubes were desorbed at 270°C, 40 mL/min He flow, with recollection on a secondary Tenax cold trap at 10°C. Analytes were released from the secondary trap by heating to 295°C with 20% transferred to the GC/MS. The GC had a 30 m length x 0.25 mm ID x 0.25 µm film thickness DB-5 column (Agilent). The GC oven was programmed to hold at 40°C for 3 min, ramp 5°C/min to 200°C, then ramp 10°C/min to 250°C, final ramp 25°C/min to 300°C, then hold at 300°C for 3 min. The TOFMS had a sampling frequency of 50 Hz and a mass recording range of 34-400 amu.
Instrument Name:Leco Pegasus 4D GC
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Flow Rate:1.2 mL/min
Chromatography Type:GC

MS:

MS ID:MS001330
Analysis ID:AN001440
Instrument Name:Leco Pegasus 4D GCxGC TOF
Instrument Type:GC x GC-TOF
MS Type:EI
Ion Mode:POSITIVE
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