Summary of study ST000903

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000627. The data can be accessed directly via it's Project DOI: 10.21228/M8MT2H This work is supported by NIH grant, U2C- DK119886.

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Study IDST000903
Study TitlePharmacometabolomic analysis of rat hippocampus and plasma in response to chronic stress and albiflorin treament
Study TypeAnimal study
Study SummaryMale Sprague-Dawley (SD) rats were exposed to one random stressor per day for 35 d. Following four weeks of stress exposure, rats with stress-induced depression-like behaviors were treated daily with either saline, fluoxetine or albiflorin for 7 days via intragastric gavage. Rat heparinized plasma was collected after drug treatment. After behavior tests, rats were sacrificed and the hippocampus was collected. Pharmacometabolomic analysis was conducted for both plasma and hippocampal samples
Institute
Beijing Wonner Biotech. Ltd. Co.
Last NameZhang
First NameZuoguang
AddressNo.9, Kexing Road, Fengtai, Beijing, Beijing, 101111, China
Emailleekefeng@hotmail.com
Phone86-10-65670787
Submit Date2017-11-16
Num Groups4
Study CommentsAnimal study
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
Zuoguang Zhang Zuoguang Zhang
https://dx.doi.org/10.21228/M8MT2H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000627
Project DOI:doi: 10.21228/M8MT2H
Project Title:Metabolomic changes of chronic stressed rats in response to albiflorin treatment
Project Summary:Depression is a complex and multifactorial disorder with various co-morbid symptoms. Current pharmacotherapies are limited by slow onset, low efficacy, and distressing side effects. Natural products are a robust source of new drugs. To understand the mechanisms of action of albiflorin, we examined the changes in global patterns of metabolism in the hippocampus and plasma of chronic stressed rats after albiflorin treatment using metabolomics
Institute:Beijing Wonner Biotech. Ltd. Co.
Last Name:Zhang
First Name:Zuoguang
Address:No.9, Kexing Road, Fengtai, Beijing, Beijing, 101111, China
Email:leekefeng@hotmail.com
Phone:86-10-65670787

Subject:

Subject ID:SU000940
Subject Type:Animal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Genotype Strain:Sprague-Dawley
Weight Or Weight Range:180-220 g
Gender:Male
Animal Animal Supplier:Beijing Huafukang Bioscience
Animal Light Cycle:12:12 light-dark cycle
Animal Feed:Standard food
Animal Water:Free access to food and water
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Treatment
SA052924Beta_10_4CUMS model Albiflorin
SA052925Beta_10_3CUMS model Albiflorin
SA052926Beta_10_1CUMS model Albiflorin
SA052927Beta_10_5CUMS model Albiflorin
SA052928Beta_10_2CUMS model Albiflorin
SA052929Beta_10_6CUMS model Albiflorin
SA052930Beta_10_8CUMS model Albiflorin
SA052931Beta_10_7CUMS model Albiflorin
SA052932Beta_9_4CUMS model Fluoxetine
SA052933Beta_9_3CUMS model Fluoxetine
SA052934Beta_9_5CUMS model Fluoxetine
SA052935Beta_9_2CUMS model Fluoxetine
SA052936Beta_9_7CUMS model Fluoxetine
SA052937Beta_9_1CUMS model Fluoxetine
SA052938Beta_9_8CUMS model Fluoxetine
SA052939Beta_9_6CUMS model Fluoxetine
SA052940Beta_8_9CUMS model Saline
SA052941Beta_8_4CUMS model Saline
SA052942Beta_8_3CUMS model Saline
SA052943Beta_8_1CUMS model Saline
SA052944Beta_8_5CUMS model Saline
SA052945Beta_8_2CUMS model Saline
SA052946Beta_8_6CUMS model Saline
SA052947Beta_8_8CUMS model Saline
SA052948Beta_8_7CUMS model Saline
SA052949Beta_7_15Wild-type Saline
SA052950Beta_7_13Wild-type Saline
SA052951Beta_7_3Wild-type Saline
SA052952Beta_7_12Wild-type Saline
SA052953Beta_7_7Wild-type Saline
SA052954Beta_7_11Wild-type Saline
SA052955Beta_7_8Wild-type Saline
SA052956Beta_7_4Wild-type Saline
Showing results 1 to 33 of 33

Collection:

Collection ID:CO000934
Collection Summary:Male Sprague-Dawley (SD) rats (180-220 g) were randomly assigned to control or CUMS group. Rats in CUMS group were exposed to one random stressor per day for 35 d. Following four weeks of stress exposure, rats with stress-induced depression-like behaviors were treated daily with either saline, Fluoxetine or Albiflroin for 7 days via intragastric gavage.
Sample Type:Plasma
Storage Conditions:-80C
Blood Serum Or Plasma:Plasma

Treatment:

Treatment ID:TR000954
Treatment Summary:Following four weeks of stress exposure, rats with stress-induced depression-like behaviors were treated daily with either saline, fluoxetine or albiflorin for 7 days via intragastric gavage
Treatment:Antidepressants
Treatment Compound:Albiflorin or fluoxetine
Treatment Route:Intragastric gavage
Treatment Dose:Albiflorin 7 mg/kg; fluoxetine 10 mg/kg
Treatment Dosevolume:0.2 ml/g
Treatment Doseduration:7 days
Treatment Vehicle:Saline
Animal Anesthesia:No
Animal Acclimation Duration:No
Animal Fasting:No

Sample Preparation:

Sampleprep ID:SP000947
Sampleprep Summary:Briefly, for plasma samples, 95 µl of plasma was mixed with 5 µl of isotope standards mix and extracted with 400 µl of pre-cold extraction buffer containing methanol/acetonitrile (50/50, v/v). The mixture was incubated on ice for 30 min and centrifuged for 10 min at 16,000 g and 4 ºC. The supernatant containing both polar and nonpolar metabolites was transferred to new tubes and stored at -80 ºC for metabolomic analysis
Extraction Method:methanol/acetonitrile (50/50, v/v) precipitation
Extract Storage:-80 ºC
Sample Derivatization:No
Sample Spiking:Isotope standards

Combined analysis:

Analysis ID AN001469 AN001470
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Waters ACQUITY UPLC Waters ACQUITY UPLC
Column Luna NH2 aminopropyl HPLC column Luna NH2 aminopropyl HPLC column
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 6500 QTrap ABI Sciex 6500 QTrap
Ion Mode NEGATIVE POSITIVE
Units AUC AUC

Chromatography:

Chromatography ID:CH001033
Instrument Name:Waters ACQUITY UPLC
Column Name:Luna NH2 aminopropyl HPLC column
Column Pressure:2200 psi
Column Temperature:25 °C
Flow Rate:0.35 ml/min
Injection Temperature:4 °C
Sample Injection:12 µl
Solvent A:95% watrer+5% acetonitrile+20 mM ammonium hydroxide+20 mM ammonium acetate, pH 9.0
Solvent B:100% acetonitrile
Sample Loop Size:12
Randomization Order:Yes
Chromatography Type:HILIC

MS:

MS ID:MS001357
Analysis ID:AN001469
Instrument Name:ABI Sciex 6500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Collision Energy:Compound dependent
Collision Gas:25
Mass Accuracy:0.1 Da
Source Temperature:475 ºC
Spray Voltage:-4500 ºC
  
MS ID:MS001358
Analysis ID:AN001470
Instrument Name:ABI Sciex 6500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Collision Energy:Compound dependent
Collision Gas:25
Mass Accuracy:0.1 Da
Source Temperature:475 ºC
Spray Voltage:5500 ºC
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