Summary of Study ST000907

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000629. The data can be accessed directly via it's Project DOI: 10.21228/M8C972 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000907
Study TitleMurine vitamin A deficiency results in a hypermetabolic state and alterations in bacterial community structure and metabolism.(Urine)
Study SummaryVitamin A deficiency (A-) is a significant public health problem. To better understand how vitamin A status influences gut microbiota and host metabolism, we systematically analyzed urine, cecum, serum, and liver samples from vitamin A sufficient (A+) and A- mice using 1H NMR-based metabolomics, quantitative (q)PCR, and 16S rRNA gene sequencing coupled with multivariate data analysis. The microbiota in the cecum of A- mice showed compositional as well as functional shifts compared to the microbiota from A+ mice. Targeted 1H NMR analyses revealed significant changes in microbial metabolite concentrations including higher butyrate and hippurate and decreased acetate and 4-hydroxyphenylacetate in A+ relative to A- mice. Bacterial butyrate-producing genes including butyryl-CoA:acetate CoA-transferase and butyrate kinase were significantly higher in bacteria from A+ versus bacteria from A- mice. A - mice had disturbances in multiple metabolic pathways including alterations in energy metabolism (hyperglycemia, glycogenesis, TCA cycle, and lipoprotein biosynthesis) and the A- host showed metabolites indicative of a hypermetabolic state (higher levels of amino acids and nucleic acids). A- mice had hyperglycemia, liver dysfunction, changes in bacterial metabolism, and altered gut microbial communities. Moreover, integrative analyses indicated a strong correlation between gut microbiota and host energy metabolism pathways in the liver. Vitamin A regulates the microbiota, bacterial metabolism and the effects of vitamin A on the microbiota results in alterations to host metabolism.
Institute
Pennsylvania State University
Last NameNichols
First NameRobert
Address101 Life science building, University park, PA, 16803
Emailrgn5011@psu.edu
Phone17247662694
Submit Date2017-09-26
Analysis Type DetailNMR
Release Date2018-02-07
Release Version1
Robert Nichols Robert Nichols
https://dx.doi.org/10.21228/M8C972
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000629
Project DOI:doi: 10.21228/M8C972
Project Title:Murine vitamin A deficiency results in a hypermetabolic state and alterations in bacterial community structure and metabolism
Project Summary:Vitamin A deficiency (A-) is a significant public health problem. To better understand how vitamin A status influences gut microbiota and host metabolism, we systematically analyzed urine, cecum, serum, and liver samples from vitamin A sufficient (A+) and A- mice using 1H NMR-based metabolomics, quantitative (q)PCR, and 16S rRNA gene sequencing coupled with multivariate data analysis. The microbiota in the cecum of A- mice showed compositional as well as functional shifts compared to the microbiota from A+ mice. Targeted 1H NMR analyses revealed significant changes in microbial metabolite concentrations including higher butyrate and hippurate and decreased acetate and 4-hydroxyphenylacetate in A+ relative to A- mice. Bacterial butyrate-producing genes including butyryl-CoA:acetate CoA-transferase and butyrate kinase were significantly higher in bacteria from A+ versus bacteria from A- mice. A - mice had disturbances in multiple metabolic pathways including alterations in energy metabolism (hyperglycemia, glycogenesis, TCA cycle, and lipoprotein biosynthesis) and the A- host showed metabolites indicative of a hypermetabolic state (higher levels of amino acids and nucleic acids). A- mice had hyperglycemia, liver dysfunction, changes in bacterial metabolism, and altered gut microbial communities. Moreover, integrative analyses indicated a strong correlation between gut microbiota and host energy metabolism pathways in the liver. Vitamin A regulates the microbiota, bacterial metabolism and the effects of vitamin A on the microbiota results in alterations to host metabolism.
Institute:Pennsylvania State University
Last Name:Nichols
First Name:Robert
Address:101 Life science building, University Park, State college, PA, 16803
Email:rgn5011@psu.edu
Phone:7247662694

Subject:

Subject ID:SU000944
Subject Type:mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA053040C1Control
SA053041C6Control
SA053042C5Control
SA053043C2Control
SA053044C3Control
SA053045C4Control
SA053046T6Vit-A deficient food
SA053047T5Vit-A deficient food
SA053048T1Vit-A deficient food
SA053049T2Vit-A deficient food
SA053050T3Vit-A deficient food
SA053051T4Vit-A deficient food
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000938
Collection Summary:urine
Sample Type:Urine

Treatment:

Treatment ID:TR000958
Treatment Summary:Twelve male litters were weaned at 3 wks and continuously fed the vitamin A sufficient diet, vitamin A deficient diet, or the vitamin A deficient diet, supplemented with retenoic acid until the end of the experiment.

Sample Preparation:

Sampleprep ID:SP000951
Sampleprep Summary:The NMR sample prep for the urine, liver, cecal contents and serum are attached.

Analysis:

Analysis ID:AN001474
Analysis Type:NMR
Num Factors:2
Num Metabolites:5
Units:total peak intensity per metabolite

NMR:

NMR ID:NM000113
Analysis ID:AN001474
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:600 MHz
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