Summary of study ST001051

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000704. The data can be accessed directly via it's Project DOI: 10.21228/M8P96G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001051
Study TitleHigh Salt Diet versus Low Salt Diet in rats
Study SummaryTwo groups of rats were fed either a high salt diet or a low salt diet. This study aims to look at salt intake in correlation to altering other metabolites and the onset of hypertension
Institute
University of Toledo
Last NameChakraborty
First NameSaroj
Address3000 Arlington Ave. Toledo, Ohio 43614-2598
EmailSaroj.Chakraborty@rockets.utoledo.edu
Phone419.383.5174
Submit Date2018-09-12
Analysis Type DetailGC-MS
Release Date2018-10-10
Release Version1
Saroj Chakraborty Saroj Chakraborty
https://dx.doi.org/10.21228/M8P96G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000704
Project DOI:doi: 10.21228/M8P96G
Project Title:Untargetted MS analysis for Diet Comparison in Rats
Project Summary:Primary metabolism was analyzed in 18 rat samples using untargetted GC-TOF MS analysis.
Institute:University of California, Davis
Last Name:Heil
First Name:Hailey
Address:451 Health Science Drive Davis CA 95616
Email:hoheil@ucdavis.edu
Phone:9167471106

Subject:

Subject ID:SU001093
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Diet
SA071391HS_S12549High Salt
SA071392HS_S12550High Salt
SA071393HS_S12542High Salt
SA071394HS_S12547High Salt
SA071395HS_S12548High Salt
SA071396HS_S12543High Salt
SA071397HS_S12545High Salt
SA071398HS_S12544High Salt
SA071399HS_S12546High Salt
SA071400LS_S12560Low Salt
SA071401LS_S12561Low Salt
SA071402LS_S12562Low Salt
SA071403LS_S12559Low Salt
SA071404LS_S12554Low Salt
SA071405LS_S12555Low Salt
SA071406LS_S12556Low Salt
SA071407LS_S12557Low Salt
SA071408LS_S12558Low Salt
Showing results 1 to 18 of 18

Collection:

Collection ID:CO001087
Collection Summary:Blood was collected from rats after completing either the high salt or low salt diet for a number of days. Rats fasted overnight before blood collection. Blood was then processed to submit only the plasma for GC-MS analysis.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR001107
Treatment Summary:18 rats were placed into either a high salt diet group (2.0% NaCl) or a low salt diet group (0.3% NaCl), to create 2 groups of 9 rats each. The diet began at day 32 of life and continued for a number of days.

Sample Preparation:

Sampleprep ID:SP001100
Sampleprep Summary:Samples were vortexed for 3 seconds to homogenize. Added 30 µL of each sample to a 1.5 mL eppendorf tube. Added 1 mL of extraction solvent (3:3:2 ACN:IPA:Water). Vortexed for 10 seconds. Shake for 6 minutes at 4°C. Centrifuged for 2 minutes at 14000 rcf. Aliquoted 2 x 475 µL portions of the supernatant into new tubes. Save one aliquote at -20°C for backup and dry the other in a centrivap to complete dryness. After the sample is dry, add 500 µL of 50:50 ACN:IPA. Centifuged for 2 minutes at 14000 rcf. Aliquoted 475 µL of supernatant to a new tube. Evaporate to complete dryness in centrivap. Submit for derivitzation.

Combined analysis:

Analysis ID AN001720
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Leco Pegasus HT TOF
Ion Mode POSITIVE
Units Peak Height

Chromatography:

Chromatography ID:CH001216
Instrument Name:Agilent 7890A
Column Name:Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
Column Temperature:50-330C
Flow Rate:1 mL min^(-1)
Injection Temperature:50°C ramped to 250°C by 12°C s-1
Oven Temperature:50°C for 1 min, then ramped at 20°C min-1 to 330°C, held constant for 5 min
Chromatography Type:GC

MS:

MS ID:MS001592
Analysis ID:AN001720
Instrument Name:Leco Pegasus HT TOF
Instrument Type:GC-TOF
MS Type:EI
Ion Mode:POSITIVE
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