Summary of Study ST001053

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000706. The data can be accessed directly via it's Project DOI: 10.21228/M8DT2N This work is supported by NIH grant, U2C- DK119886.

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Study IDST001053
Study Title1H-NMR Analysis of Skin and Blubber of Nose Dolphin Metabolome Reveal the Functional Metabolomic Dichotomy of The Organs
Study TypeDolphin Skin and Blubber
Study SummaryThe common bottlenose dolphin (Tursiops truncatus) is carnivorous cetacean thriving in marine environment are one of the most common apex predators found in coastal and estuarine ecosystems. Although recent studies have focused on capturing the circulating metabolomes of these organisms, with respect to pollutants and exposures of the marine environment, the skin and blubber are important protective organs that have not been probed. Using 1HNMR based untargeted metabolomics we quantified 51 metabolites belonging to 74 different metabolic pathways in the skin and blubber of bottle nose dolphins (n=5) samples collected in 2017 from the coast of Mexico. Results indicate that the skin and blubber metabolism are quantitatively different. These metabolite abundances could help discriminate the tissue-types using supervised and unsupervised PCA and PLSDA analysis. Heat maps and random forest analysis point to unique metabolites that are important classifiers of the tissue-type. The altered metabolic patterns, mainly linking fatty acid metabolism and ketogenic amino acids, seem to constitute a characteristic of blubber, while the skin showed diverse metabolites involved in gluceoneogenic pathways. 1H NMR spectra allowed the identification of metabolites associated with these organ types, such as pyruvic acid, arginine, ornithine, 2-hydroxybutyric acid, 3-hydroxyisobutyric acid, and acetic acid, as discriminatory and classifying metabolites. These results would lead to further understanding of dolphin skin and blubber metabolism for better efforts in their conservation as well as a measure of marine pollution and ecotoxicology.
Institute
Wake Forest Baptist Medical Center
DepartmentDepartment of Internal Medicine
Last NameMisra
First NameBiswapriya
AddressMedical Center Boulevard NRC Building, G#43, Medical Center Boulevard, Winston Salem, NC, 27157, USA
Emailbbmisraccb@gmail.com
Phone3522156040
Submit Date2018-09-12
Num Groups2
Total Subjects4
Num Males3
Num Females1
Study CommentsNA
PublicationsDOI: https://doi.org/10.1016/j.cbd.2019.02.004 1H-NMR Metabolomic Analysis of Skin and Blubber of Bottlenose Dolphins Reveal a Functional Metabolic Dichotomy, Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics Author List: Biswapriya Misra, Ixchel Ruiz-Hernández, Gloria Ivonne Hernández-Bolio, Emanuel Hernandez Nuñez, Raúl Díaz-Gamboa, Reyna Cristina Collí Dulá
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2019-12-17
Release Version1
Biswapriya Misra Biswapriya Misra
https://dx.doi.org/10.21228/M8DT2N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000706
Project DOI:doi: 10.21228/M8DT2N
Project Title:Skin and Blubber Metabolomics
Project Type:Multi-institutional Metabolomics
Project Summary:The common bottlenose dolphin (Tursiops truncatus) is carnivorous cetacean thriving in marine environment are one of the most common apex predators found in coastal and estuarine ecosystems. Although recent studies have focused on capturing the circulating metabolomes of these organisms, with respect to pollutants and exposures of the marine environment, the skin and blubber are important protective organs that have not been probed. Using 1HNMR based untargeted metabolomics we quantified 51 metabolites belonging to 74 different metabolic pathways in the skin and blubber of bottle nose dolphins (n=5) samples collected in 2017 from the coast of Mexico. Results indicate that the skin and blubber metabolism are quantitatively different. These metabolite abundances could help discriminate the tissue-types using supervised and unsupervised PCA and PLSDA analysis. Heat maps and random forest analysis point to unique metabolites that are important classifiers of the tissue-type. The altered metabolic patterns, mainly linking fatty acid metabolism and ketogenic amino acids, seem to constitute a characteristic of blubber, while the skin showed diverse metabolites involved in gluceoneogenic pathways. 1H NMR spectra allowed the identification of metabolites associated with these organ types, such as pyruvic acid, arginine, ornithine, 2-hydroxybutyric acid, 3-hydroxyisobutyric acid, and acetic acid, as discriminatory and classifying metabolites. These results would lead to further understanding of dolphin skin and blubber metabolism for better efforts in their conservation as well as a measure of marine pollution and ecotoxicology.
Institute:Wake Forest Baptist Medical Center
Department:Department of Internal Medicine
Last Name:Misra
First Name:Biswapriya
Address:Medical Center Boulevard NRC Building, G#43, Medical Center Boulevard, Winston Salem, NC, 27157, USA
Email:bbmisraccb@gmail.com
Phone:3522156040
Funding Source:NA
Project Comments:NA
Publications:In process
Contributors:Biswapriya B. Misra, Ixchel Mariel Ruiz Hernandez, Gloria Ivonne Hernández Bolio, Emanuel Hernandez, Colli Dula Reyna Cristina

Subject:

Subject ID:SU001095
Subject Type:Mammal
Subject Species:Tursiops truncatus
Taxonomy ID:9739
Gender:Male and female

Factors:

Subject type: Mammal; Subject species: Tursiops truncatus (Factor headings shown in green)

mb_sample_id local_sample_id Tissue
SA071462G15_1Blubber
SA071463G15_2Blubber
SA071464G7_1Blubber
SA071465G14_2Blubber
SA071466G15_3Blubber
SA071467G14_3Blubber
SA071468G14_1Blubber
SA071469G7_2Blubber
SA071470G10_1Blubber
SA071471G7_3Blubber
SA071472G10_2Blubber
SA071473G10_3Blubber
SA071474P15_1Skin
SA071475P14_3Skin
SA071476P15_3Skin
SA071477P14_2Skin
SA071478P15_2Skin
SA071479P7_1Skin
SA071480P7_3Skin
SA071481P7_2Skin
SA071482P10_1Skin
SA071483P10_2Skin
SA071484P10_3Skin
SA071485P14_1Skin
Showing results 1 to 24 of 24

Collection:

Collection ID:CO001089
Collection Summary:Environmental samples.
Sample Type:Skin and Blubber
Collection Method:NA
Collection Tube Temp:4 C

Treatment:

Treatment ID:TR001109
Treatment Summary:None.

Sample Preparation:

Sampleprep ID:SP001102
Sampleprep Summary:A portion (about 20 mg) of each tissue extract was dissolved in 500 ml of phosphate buffer (pH = 7.0) and then 500 ml of deuterium oxide containing 0.05 % v/v trimethylsilane was added.

Analysis:

Analysis ID:AN001722
Laboratory Name:Laboratorio Nacional de Nano y Biomateriales, CINVESTAV-Unidad Mérida
Analysis Type:NMR
Acquisition Date:June 13, 2018
Software Version:Vnmrj 2.3, Revision A
Operator Name:Dra. Gloria Ivonne Hernández Bolio
Detector Type:NA
Data Format:.fid
Num Factors:2
Num Metabolites:51
Units:Relative abundance

NMR:

NMR ID:NM000128
Analysis ID:AN001722
Instrument Name:Varian 600 MHz AR Premium Compact
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:600 MHz
NMR Probe:OneProbe
NMR Solvent:D2O
NMR Tube Size:5 mm
Shimming Method:Gradient shimming
Pulse Sequence:PRESAT
Water Suppression:Yes, satmode
Pulse Width:5.90 (90°)
Power Level:63
Receiver Gain:20
Presaturation Power Level:20
Chemical Shift Ref Cpd:0 ppm (TSP)
Temperature:25
Number Of Scans:128
Acquisition Time:3
Relaxation Delay:2
Spectral Width:9615.4
Num Data Points Acquired:64000
Real Data Points:57692
Chemical Shift Ref Std:TSP
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