Summary of study ST001071

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000717. The data can be accessed directly via it's Project DOI: 10.21228/M80M50 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001071
Study TitleGuinea Grass Untargeted Metabolomic Profiling
Study TypeTreatment comparison
Study SummaryTreatment comparison of Guinea grass planted at University of Sao Paulo Brazil 4 Ambient temp and CO2 plots, 4 elevated CO2 (600 ppm) plots, 4 elevated Temp plots (+2C),4 elevated CO2 and Temp plots. Untargeted metabolomics(GC-MS),de novo transcriptomics, and RNAseq taken at 30(A)(2014-05-22) and 50(B)(2014-07-14)days post treatment exposure. Dried leaf material sent to Uni Illinois-UC. GC-MS protocol followed as stated in Ulanov et al. (2010) J of Plant Phys.
Institute
University of Illinois Urbana-Champaign
DepartmentPlant Biology
LaboratoryAinsworth USDA ARS Global Change and Photosynthesis Research Unit
Last NameAinsworth
First NameElizabeth
Address1201 W Gregory Dr, Urbana, IL 61801
Emaillisa.ainsworth@ars.usda.gov
Phone217-265-9887
Submit Date2018-10-09
Num Groups4
Total Subjects32 (+ 4 techincal replicates)
Analysis Type DetailGC-MS
Release Date2018-11-08
Release Version1
Elizabeth Ainsworth Elizabeth Ainsworth
https://dx.doi.org/10.21228/M80M50
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000717
Project DOI:doi: 10.21228/M80M50
Project Title:Guinea Grass elevated CO2 and warming Project
Project Type:Elevated temperature and CO2 treatment
Project Summary:Experiment to test the response of Panicum maximum Jacq.(Guinea Grass) to elevated temperature (+2C) and CO2 (600ppm) or combination at University of Sao Paulo in Ribeirao Preto, Sao Paulo Brazil. Each plot was 2-m in diameter; [CO2] was controlled using Free Air CO2 Enrichment and canopy warming was provided by infrared ceramic heaters as described by Britto de Assis Prado et al. (2016).Samples for untargeted metabolomics (with GC-MS), de novo transcriptomics, and RNASeq, were taken 30 and 50 days post treatment exposure (May 22, 2014 and July 14, 2014). Lyophilized leaf material was sent to the University of Illinois Urbana-Champaign(Ainsworth lab) for the untargeted metabolomics, transcript assembly and RNASeq.
Institute:University of Sao Paulo
Department:Department of Biology-FFCLRP
Laboratory:Martinez
Last Name:Martinez
First Name:Carlos Alberto
Address:Ribeirao Preto, SP, Brazil
Email:carlosamh@ffclrp.usp.br
Phone:+55 16 3315 3648
Funding Source:FAPESP- Research Program on Global Climate Change (Grant 2008/58075-8) - CNPq/ANA/MCTI (Grant 446357/2015-4)
Publications:Leaf Dynamics of Panicum maximum under future climate changes-https://doi.org/10.1371/journal.pone.0149620
Contributors:Jessica M. Wedow, Craig R. Yendrek, Tathyana R. Mello, Silvana Creste, Carlos A. Martinez, Elizabeth A. Ainsworth

Subject:

Subject ID:SU001115
Subject Type:Plant
Subject Species:Panicum maximum(Megathyrsis maximus (Jacq.))
Taxonomy ID:59788

Factors:

Subject type: Plant; Subject species: Panicum maximum(Megathyrsis maximus (Jacq.)) (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Ring Time Point (days)
SA072348Amb_R1_T1Control- ambient temperature and ambient [CO2] 1 30
SA072349Amb_R1_T2Control- ambient temperature and ambient [CO2] 1 50
SA072350Amb_R2_T1Control- ambient temperature and ambient [CO2] 2 30
SA072351Amb_R2_T2Control- ambient temperature and ambient [CO2] 2 50
SA072352Amb_R3_T1Control- ambient temperature and ambient [CO2] 3 30
SA072353Amb_R3_T2Control- ambient temperature and ambient [CO2] 3 50
SA072354Amb_R4_T1Control- ambient temperature and ambient [CO2] 4 30
SA072355Amb_R4_T2Control- ambient temperature and ambient [CO2] 4 50
SA072356eC_R1_T1elevated [CO2] + ambient temperature 1 30
SA072357eC_R1_T2elevated [CO2] + ambient temperature 1 50
SA072358eC_R2_T1elevated [CO2] + ambient temperature 2 30
SA072359eC_R2_T2elevated [CO2] + ambient temperature 2 50
SA072360eC_R3_T1elevated [CO2] + ambient temperature 3 30
SA072361eC_R3_T2elevated [CO2] + ambient temperature 3 50
SA072362eC_R4_T1elevated [CO2] + ambient temperature 4 30
SA072363eC_R4_T2elevated [CO2] + ambient temperature 4 50
SA072364T_R1_T1elevated temperature + ambient [CO2] 1 30
SA072365T_R1_T2elevated temperature + ambient [CO2] 1 50
SA072366T_R2_T1elevated temperature + ambient [CO2] 2 30
SA072367T_R2_T2elevated temperature + ambient [CO2] 2 50
SA072368T_R3_T1elevated temperature + ambient [CO2] 3 30
SA072369T_R3_T2elevated temperature + ambient [CO2] 3 50
SA072370T_R4_T1elevated temperature + ambient [CO2] 4 30
SA072371T_R4_T2elevated temperature + ambient [CO2] 4 50
SA072372eCeT_R1_T1elevated temperature + elevated [CO2] 1 30
SA072373eCeT_R1_T2elevated temperature + elevated [CO2] 1 50
SA072374eCeT_R2_T1elevated temperature + elevated [CO2] 2 30
SA072375eCeT_R2_T2elevated temperature + elevated [CO2] 2 50
SA072376eCeT_R3_T1elevated temperature + elevated [CO2] 3 30
SA072377eCeT_R3_T2elevated temperature + elevated [CO2] 3 50
SA072378eCeT_R4_T1elevated temperature + elevated [CO2] 4 30
SA072379eCeT_R4_T2elevated temperature + elevated [CO2] 4 50
Showing results 1 to 32 of 32

Collection:

Collection ID:CO001109
Collection Summary:Leaf material dried and processed as stated Ulanov et al. (2010) J of Plant Phys
Sample Type:Plant
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001129
Treatment Summary:Ambient(Amb) : Control plots with ambient [CO2] and ambient temperature elvTemp(T): elevated temperature plots (+2C) and ambient [CO2] elvCO2(eC): elevated [CO2] plots (600ppm) and ambient temperature elvTemp+elvCO2(eCeT): elevated temperature (+2C) and elevated [CO2] (600ppm). Time Point A(2014-05-22) and Time Point B(2014-07-14)
Treatment Protocol Comments:Time Point A(2014-05-22) and Time Point B(2014-07-14)
Treatment:elevated [CO2] and/or elevated temperature
Treatment Doseduration:30 days and 50days
Plant Growth Location:MiniFACE facility at University of São Paul in Ribeirão Preto, São Paulo Brazil
Plant Plot Design:Complete replicated design (n=4)
Plant Light Period:ambient
Plant Harvest Date:2014-05-22
Plant Metab Quench Method:Immersion in liquid N2 within 1 min of harvest
Plant Harvest Method:Youngest fully expanded leaves was cut (PO:0001053), placed in foil, quenched in LN2
Plant Storage:-80 C, then lyophilized and ground, shipped on dry ice to University of Illinois at Urbana-Champagin were stored in 20 C in falcon tubes

Sample Preparation:

Sampleprep ID:SP001122
Sampleprep Summary:Room temp Methanol extration: 10-20 mg leaf material, extracted with 1.5 mL MeOH for 15 min at RT followed by another extraction with 1.4 mL H2O at RT. 1 mL of extraction dried under vacuum. 5 uL internal standard added to each sample. 1uL aliquot per sample
Sampleprep Protocol Comments:As described in Ulanov, A. & J. M. Widholm (2010) Metabolic profiling to determine the cause of the increased triphenyltetrazolium chloride reduction in mannitol-treated maize callus. Journal of Plant Physiology, 167, 1423-1431.
Processing Method:Homogenization and Solvent removal with speed vac
Processing Storage Conditions:Room temperature
Extraction Method:1:1 MeOH/H20
Extract Storage:-80℃
Sample Spiking:Internal Standard (hentriacontanoic acid 5 uL)

Combined analysis:

Analysis ID AN001753
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890A
Column Phenomenea ZB-5MS (60m x 0.32 mmI.D. and 0.25mm film thickness)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975C
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH001237
Instrument Name:Agilent 7890A
Column Name:Phenomenea ZB-5MS (60m x 0.32 mmI.D. and 0.25mm film thickness)
Column Pressure:19.223 psi
Flow Rate:2.4ml/min
Injection Temperature:250C
Internal Standard:C31:0 fatty acid (hentriacontanoate)
Sample Injection:split (5:1), 1ul
Analytical Time:65 min
Oven Temperature:70C - 310C
Running Voltage:1094 emvolts
Transferline Temperature:250
Strong Wash Solvent Name:pyridine
Strong Wash Volume:10ul
Sample Syringe Size:10ul
Chromatography Type:GC

MS:

MS ID:MS001621
Analysis ID:AN001753
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Helium Flow:2.4
Ion Source Temperature:230
Ionization Energy:69.9
Mass Accuracy:low
Scan Range Moverz:50-800
Scanning Cycle:4
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