Summary of Study ST001078

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000721. The data can be accessed directly via it's Project DOI: 10.21228/M8GM4Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST001078
Study TitleMulti-platform metabolomics data from Ndufs4-/- skeletal muscles (part-V)
Study TypeNMR analysis of Ndufs4-/- white quadriceps muscles
Study SummaryNMR analysis of Ndufs4-/- and WT mouse white quadriceps muscles
Institute
North-West University
Last NameLouw
First NameRoan
AddressHofman Street
EmailRoan.Louw@nwu.ac.za
Phone+27 18 299 4074
Submit Date2018-10-18
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2019-01-22
Release Version1
Roan Louw Roan Louw
https://dx.doi.org/10.21228/M8GM4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000721
Project DOI:doi: 10.21228/M8GM4Z
Project Title:Multi-platform metabolomics data from Ndufs4-/- skeletal muscles
Project Type:Multi-platform metabolomics study
Project Summary:Multi-platform metabolomics analysis of white quadriceps and soleus skeletal muscles from the Ndufs4 knockout (Ndufs4-/-) mouse model of human Leigh syndrome.
Institute:North-West University
Last Name:Louw
First Name:Roan
Address:Hofman Street
Email:Roan.Louw@nwu.ac.za
Phone:+27 18 299 4074

Subject:

Subject ID:SU001122
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:Ndufs4, https://www.jax.org/strai n/02705 8
Age Or Age Range:45-50 days
Gender:Male
Animal Animal Supplier:Jackson Laboratory (ME, USA)
Animal Light Cycle:12:12 h
Animal Feed:Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub
Animal Water:ad libitum

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA072603MQ_17Ndufs4-/-
SA072604MQ_19Ndufs4-/-
SA072605MQ_21Ndufs4-/-
SA072606MQ_14Ndufs4-/-
SA072607MQ_8Ndufs4-/-
SA072608MQ_4Ndufs4-/-
SA072609MQ_5Ndufs4-/-
SA072610MQ_23Ndufs4-/-
SA072611MQ_10Ndufs4-/-
SA072612MQ_24Ndufs4-/-
SA072613MQ_37Ndufs4-/-
SA072614MQ_38Ndufs4-/-
SA072615MQ_40Ndufs4-/-
SA072616MQ_35Ndufs4-/-
SA072617MQ_34Ndufs4-/-
SA072618MQ_26Ndufs4-/-
SA072619MQ_29Ndufs4-/-
SA072620MQ_2Ndufs4-/-
SA072621MQ_39WT
SA072622MQ_13WT
SA072623MQ_15WT
SA072624MQ_16WT
SA072625MQ_11WT
SA072626MQ_9WT
SA072627MQ_3WT
SA072628MQ_6WT
SA072629MQ_7WT
SA072630MQ_18WT
SA072631MQ_20WT
SA072632MQ_32WT
SA072633MQ_33WT
SA072634MQ_36WT
SA072635MQ_31WT
SA072636MQ_30WT
SA072637MQ_22WT
SA072638MQ_25WT
SA072639MQ_28WT
SA072640MQ_1WT
Showing results 1 to 38 of 38

Collection:

Collection ID:CO001116
Collection Summary:Mice were euthanized between postnatal day (P) 45-50 via cervical dislocation at the same time of day (8:00-9:00 AM) after overnight (12-h) fasting. From both hind limbs, white muscle portions of quadriceps femoris, as well as soleus muscles were collected. Tissues were snap-frozen in liquid nitrogen (within 15 min post-mortem) and stored at -80°C until used.
Sample Type:Muscle

Treatment:

Treatment ID:TR001136
Treatment Summary:The animals did not receive any treatment

Sample Preparation:

Sampleprep ID:SP001129
Sampleprep Summary:Muscle tissues were finely minced and homogenized in the presence of internal standards using a vibration mill. Metabolite extraction was achieved using a modified monophasic Bligh–Dyer extraction method with a solvent ratio of 3:1:1 (methanol:water:chloroform).
Sampleprep Protocol Filename:NMR_quadriceps_sample_prep_protocol. pdf

Analysis:

Analysis ID:AN001760
Analysis Type:NMR
Analysis Protocol File:NMR_data_acquisition_method.pdf
Processing Parameters File:Data_processing_method.pdf
Num Factors:2
Num Metabolites:36
Units:Normalized peak area

NMR:

NMR ID:NM000131
Analysis ID:AN001760
Instrument Name:Bruker Avance III HD
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:500 MHz
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