Summary of Study ST001109
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000740. The data can be accessed directly via it's Project DOI: 10.21228/M81D57 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001109 |
Study Title | NMR Metabolomics of Newborn Heart Tissue Exposed to Excess Maternal Cortisol in Late Gestation - Maternal (part -IV) |
Study Type | Comparison |
Study Summary | Serum from mothers and fetuses exposed to excess maternal cortisol in late gestation and untreated was compared via NMR metabolomic analysis |
Institute | University of Florida |
Department | Biochemsitry & Molecular Biology |
Last Name | Walejko |
First Name | Jacquelyn |
Address | R3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
jwalejko@uga.edu | |
Phone | NA |
Submit Date | 2018-12-04 |
Num Groups | 2 |
Total Subjects | 36 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2019-03-06 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000740 |
Project DOI: | doi: 10.21228/M81D57 |
Project Title: | Chronic Maternal Cortisol Excess During Late Gestation Leads to Metabolic Alterations in the Newborn Heart |
Project Summary: | Our laboratory has previously shown in an ovine model of pregnancy that abnormal elevations in maternal cortisol during late gestation lead to increased fetal cardiac arrhythmias and mortality during peripartum. Furthermore, transcriptomic analysis of the fetal heart suggested alterations in TCA cycle intermediates and lipid metabolites in animals exposed to excess cortisol in utero. Therefore, we utilized a sheep model of pregnancy to determine how chronic increases in maternal cortisol alter maternal and fetal serum prior to birth and neonatal cardiac metabolites and lipids at term. Ewes were either infused with 1 mg/kg/day of cortisol starting at gestational day 115 (n=9), or untreated (n=6). Serum was collected from the mother and fetus (125 d-birth), and hearts were collected following birth. Proton nuclear magnetic resonance (1H-NMR) spectroscopy was conducted to measure metabolic profiles of newborn heart specimens as well as fetal and maternal serum specimens. Mass spectrometry was conducted to measure lipid profiles of newborn heart specimens. We observed alterations in amino acid and TCA cycle metabolism as well as lipid and glycerophospholipid metabolism in newborn hearts after excess maternal cortisol in late gestation. In addition, we observed alterations in amino acid and TCA cycle metabolites in fetal but not in maternal serum during late gestation. These results suggest that fetal exposure to excess maternal cortisol alters placental and fetal metabolism prior to birth and limits normal cardiac metabolic maturation, which may contribute to increased risk of peripartum cardiac arrhythmias observed in these animals, or later life cardiomyopathies. |
Institute: | University of Florida |
Department: | Pharmacodynamics |
Last Name: | Keller-Wood |
First Name: | Maureen |
Address: | 1345 SW Archer Rd, PO 100487, Gainesville, FL, 32610 |
Email: | kellerwd@cop.ufl.edu |
Phone: | NA |
Subject:
Subject ID: | SU001154 |
Subject Type: | Mammal |
Subject Species: | Ovis aries |
Taxonomy ID: | 9940 |
Age Or Age Range: | Gestational days 125-birth |
Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Ovis aries (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment | Source |
---|---|---|---|
SA075467 | F_1616-10_140 | Control | Fetal |
SA075468 | F_753_135 | Control | Fetal |
SA075469 | F_728_144 | Control | Fetal |
SA075470 | F_734_143 | Control | Fetal |
SA075471 | F_728_140 | Control | Fetal |
SA075472 | F_759_138 | Control | Fetal |
SA075473 | F_728_142 | Control | Fetal |
SA075474 | F_767_135 | Control | Fetal |
SA075475 | F_753_130 | Control | Fetal |
SA075476 | F_1618_147 | Control | Fetal |
SA075477 | F_759_142 | Control | Fetal |
SA075478 | F_759_145 | Control | Fetal |
SA075479 | F_734_140 | Control | Fetal |
SA075480 | F_759_130 | Control | Fetal |
SA075481 | F_734_138 | Control | Fetal |
SA075482 | F_1618_144 | Control | Fetal |
SA075483 | F_1618_142 | Control | Fetal |
SA075484 | F_734_142 | Control | Fetal |
SA075485 | F_1618_148 | Control | Fetal |
SA075486 | F_1577_125 | Control | Fetal |
SA075487 | F_753_143 | Control | Fetal |
SA075488 | F_1618_149 | Control | Fetal |
SA075489 | F_1618_145 | Control | Fetal |
SA075490 | F_1618_125 | Control | Fetal |
SA075491 | F_767_130 | Control | Fetal |
SA075492 | F_753_138 | Control | Fetal |
SA075493 | F_767_125 | Control | Fetal |
SA075494 | F_1618_150 | Control | Fetal |
SA075495 | F_1616-10_125 | Control | Fetal |
SA075496 | F_759_144 | Control | Fetal |
SA075497 | F_734_130 | Control | Fetal |
SA075498 | F_734_125 | Control | Fetal |
SA075499 | F_734_144 | Control | Fetal |
SA075500 | F_753_140 | Control | Fetal |
SA075501 | F_1618_130 | Control | Fetal |
SA075502 | F_753_125 | Control | Fetal |
SA075503 | F_759_147 | Control | Fetal |
SA075504 | F_1616-10_137 | Control | Fetal |
SA075505 | F_1618_146 | Control | Fetal |
SA075506 | F_1577_140 | Control | Fetal |
SA075507 | F_1618_138 | Control | Fetal |
SA075508 | F_759_140 | Control | Fetal |
SA075509 | F_1577_138 | Control | Fetal |
SA075510 | F_770_130 | Control | Fetal |
SA075511 | F_1577_120 | Control | Fetal |
SA075512 | F_728_146 | Control | Fetal |
SA075513 | F_1577_130 | Control | Fetal |
SA075514 | F_759_125 | Control | Fetal |
SA075515 | F_770_135 | Control | Fetal |
SA075516 | F_759_146 | Control | Fetal |
SA075517 | F_1616-10_130 | Control | Fetal |
SA075518 | F_1618_143 | Control | Fetal |
SA075519 | F_1618_140 | Control | Fetal |
SA075520 | F_728_135 | Control | Fetal |
SA075521 | F_734_135 | Control | Fetal |
SA075522 | F_728_147 | Control | Fetal |
SA075523 | F_728_130 | Control | Fetal |
SA075524 | F_1618_141 | Control | Fetal |
SA075525 | F_728_138 | Control | Fetal |
SA075526 | F_770_140 | Control | Fetal |
SA075527 | F_1618_135 | Control | Fetal |
SA075528 | F_770_125 | Control | Fetal |
SA075529 | F_770_138 | Control | Fetal |
SA075530 | F_759_135 | Control | Fetal |
SA075531 | F_1577_135 | Control | Fetal |
SA075532 | M_1577_120 | Control | Maternal |
SA075533 | M_1616-10_140 | Control | Maternal |
SA075534 | M_753_125 | Control | Maternal |
SA075535 | M_1618_140 | Control | Maternal |
SA075536 | M_767_138 | Control | Maternal |
SA075537 | M_1577_140 | Control | Maternal |
SA075538 | M_1618_141 | Control | Maternal |
SA075539 | M_1618_135 | Control | Maternal |
SA075540 | M_1618_146 | Control | Maternal |
SA075541 | M_1618_130 | Control | Maternal |
SA075542 | M_1577_130 | Control | Maternal |
SA075543 | M_1577_135 | Control | Maternal |
SA075544 | M_1577_125 | Control | Maternal |
SA075545 | M_1618_144 | Control | Maternal |
SA075546 | M_1616-10_137 | Control | Maternal |
SA075547 | M_753_130 | Control | Maternal |
SA075548 | M_753_138 | Control | Maternal |
SA075549 | M_1577_138 | Control | Maternal |
SA075550 | M_767_130 | Control | Maternal |
SA075551 | M_1616-10_130 | Control | Maternal |
SA075552 | M_1618_138 | Control | Maternal |
SA075553 | M_767_135 | Control | Maternal |
SA075554 | M_759_144 | Control | Maternal |
SA075555 | M_734_144 | Control | Maternal |
SA075556 | M_1618_145 | Control | Maternal |
SA075557 | M_734_135 | Control | Maternal |
SA075558 | M_759_147 | Control | Maternal |
SA075559 | M_728_135 | Control | Maternal |
SA075560 | M_759_145 | Control | Maternal |
SA075561 | M_1618_142 | Control | Maternal |
SA075562 | M_1618_148 | Control | Maternal |
SA075563 | M_728_130 | Control | Maternal |
SA075564 | M_770_138 | Control | Maternal |
SA075565 | M_728_147 | Control | Maternal |
SA075566 | M_759_135 | Control | Maternal |
Collection:
Collection ID: | CO001148 |
Collection Summary: | Blood samples were collected from ewe and fetus on gestational days 125, 130, 135, 138, 140, and every subsequent day until birth (control: 144±1; CORT: 142±2 days gestational age); |
Sample Type: | Blood (serum) |
Collection Method: | samples for metabolomics analysis were collected in glass tubes on ice with no anticoagulant and spun for 20 min at 4 °C; the resulting serum was stored at -20 °C until metabolomic analysis. |
Collection Location: | University of Florida |
Storage Conditions: | -20℃ |
Collection Vials: | Eppendorf tubes |
Storage Vials: | Eppendorf tubes |
Treatment:
Treatment ID: | TR001168 |
Treatment Summary: | The treatment protocol for this study was previous published in Antolic, A., et al. (2018). Chronic maternal hypercortisolemia in late gestation alters fetal cardiac function at birth. Am J Physiol Regul Integr Comp Physiol 314(3): R342-R352. |
Treatment Compound: | Hydrocortisone sodium succinate in sodium phosphate (Solu-Cortef; Pfizer, New York, NY, USA) |
Treatment Dose: | 1 mg/kg/day |
Sample Preparation:
Sampleprep ID: | SP001161 |
Sampleprep Summary: | Saline buffer was created by weighing 9 g of NaCl into a 1 L volumetric flask. Next, 100 mL of D2O was added and filled up to 1 L with water and shaken until dissolved. Samples were thawed on ice and centrifuged at 3,000 g for 20 min at 4 °C. Two hundred mL of serum was mixed with 400 mL of Saline buffer and centrifuged for 10 min at 3000 g at 4 °C. For each sample, 500 mL of the resulting supernatant was transferred into a 5 mm SampleJet NMR tube (Bruker Biospin, Billerica, MA, USA). |
Analysis:
Analysis ID: | AN001803 |
Laboratory Name: | Advanced Magnetic Resonance Imaging and Spectroscopy Facility-University of Florida |
Analysis Type: | NMR |
Analysis Protocol File: | Serum_CORT_raw_data_cpmg.zip |
Acquisition Parameters File: | Serum_CORT_raw_data_cpmg.zip |
Software Version: | Topspin 3.6 |
Operator Name: | Jacquelyn Walejko |
Processing Parameters File: | Serum_CORT_raw_data_cpmg.zip |
Num Factors: | 4 |
Num Metabolites: | 29 |
Units: | Area under curve |
NMR:
NMR ID: | NM000139 |
Analysis ID: | AN001803 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Field Frequency Lock: | H2O+D2O |
Spectrometer Frequency: | 600 MHz |
NMR Probe: | 5mm Cryoprobe |
NMR Solvent: | D2O |
NMR Tube Size: | 5mm |
Shimming Method: | Topshim |
Pulse Sequence: | cpmgpr1d |
Water Suppression: | Presaturation |
Power Level: | 7.37 W |
Receiver Gain: | 64 |
Offset Frequency: | 2824.2 |
Presaturation Power Level: | 6.41E-05 |
Temperature: | 4C |
Number Of Scans: | 128 |
Dummy Scans: | 16 |
Acquisition Time: | 1.8173 |
Relaxation Delay: | 4 sec |
Spectral Width: | 15.0182 ppm |
Num Data Points Acquired: | 32764 |
Real Data Points: | 65536 |
Line Broadening: | 1 |