Summary of Study ST001131
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000756. The data can be accessed directly via it's Project DOI: 10.21228/M8ZH5N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001131 |
| Study Title | Presentation of different serum metabolites in trauma patients versus healthy volunteers. |
| Study Summary | This study seeks to identify critical metabolic differences between patients who have undergone physical trauma and patients who have not experienced physical trauma. |
| Institute | Texas Tech University |
| Department | University Health Sciences Center |
| Last Name | Mudaliar |
| First Name | Nithya |
| Address | 3601 4th St, Lubbock, TX 79430 |
| nithya.s.mudaliar@ttuhsc.edu | |
| Phone | 8067436794 |
| Submit Date | 2019-01-28 |
| Num Groups | 2 |
| Total Subjects | 12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-03-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000756 |
| Project DOI: | doi: 10.21228/M8ZH5N |
| Project Title: | Untargeted GC-MS Analysis in human serum from trauma and healthy patients. |
| Project Summary: | Untargeted GC-MS analysis was used to analysis the serum from 6 trauma patients and 6 healthy control patients. This project aims to identify metabolic differences between the two groups. |
| Institute: | University of California, Davis |
| Department: | Genome and Biomedical Sciences Facility |
| Laboratory: | WCMC Metabolomics Core |
| Last Name: | Fiehn |
| First Name: | Oliver |
| Address: | 451 East Health Science Drive |
| Email: | ofiehn@ucdavis.edu |
| Phone: | (530) 754-8258 |
Subject:
| Subject ID: | SU001192 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA078095 | HV1_001 | Healthy |
| SA078096 | HV27_006 | Healthy |
| SA078097 | HV22_005 | Healthy |
| SA078098 | HV2_002 | Healthy |
| SA078099 | HV7_003 | Healthy |
| SA078100 | HV13_004 | Healthy |
| SA078101 | TP19_012 | Trauma |
| SA078102 | TP17_011 | Trauma |
| SA078103 | TP3_007 | Trauma |
| SA078104 | TP7_008 | Trauma |
| SA078105 | TP12_009 | Trauma |
| SA078106 | TP15_010 | Trauma |
| Showing results 1 to 12 of 12 |
Collection:
| Collection ID: | CO001186 |
| Collection Summary: | Serum samples were collected from each human after exposure to each treatment group. Samples were snap frozen and shipped to the WCMC on dry ice. Upon arrival, samples were placed in a -80°C freezer. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR001207 |
| Treatment Summary: | Samples were collected from patients who had undergone some type of physical trauma. This group consisted of six patients. Serum samples from six patients who had not experienced physical trauma, were considered health, and were of similar characteristics to the patients in the trauma group. Overall, 12 samples were collected from 2 groups of patients, comprised of six patients each. |
Sample Preparation:
| Sampleprep ID: | SP001200 |
| Sampleprep Summary: | All extraction procedures are performed over crushed ice. Samples were defrosted over crushed ice for 30 minutes or until defrosted. 30 µL of serum was aliquoted from the original sample into a 1.5 mL Eppendorf tube. 1 mL of 3:3:2 ACN:IPA:Water was added to each sample. Samples were vortexed for 10 seconds. Samples were shaken for 6 minutes at 4°C using an orbital mixing plate. Samples were centrifuged for 2 minutes at 14,000 rcf. 2 aliquots of 475 µL were removed from the supernatant and placed into new 1.5 mL Eppendorf tubes. 1 sample is frozen at -20°C and saved as a backup. The other sample is dried to completeness using a Labconco Centrivap. |
| Sample Derivatization: | To the dried down samples, 10 µL of a prepared methoxyamine solution was added to each sample. Samples were shaken on an Orbital Mixing Chilling/Heating Plate at 30°C for 1.5 hours. 91 µL of a N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA) and fatty-acid methyl-esters (FAMES) solution was added to each sample. Samples were shaken on an Orbital Mixing Chilling/Heating Plate at 37°C for 30 minutes. Contents were transferred to individual glass vials with micro-inserts and capped immediately. Submitted to GC-MS analysis. |
Combined analysis:
| Analysis ID | AN001856 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Leco Pegasus III GC TOF |
| Ion Mode | POSITIVE |
| Units | Peak Height |
Chromatography:
| Chromatography ID: | CH001343 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Column Temperature: | 50-330°C |
| Flow Rate: | 1 mL min^(-1) |
| Injection Temperature: | 50°C ramped to 250°C by 12°C s-1 |
| Oven Temperature: | 50°C for 1 min, then ramped at 20°C min-1 to 330°C, held constant for 5 min |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001716 |
| Analysis ID: | AN001856 |
| Instrument Name: | Leco Pegasus III GC TOF |
| Instrument Type: | GC-TOF |
| MS Type: | EI |
| MS Comments: | N/A |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 250°C |
| Ionization Energy: | -70 eV |
| Resolution Setting: | 17 spectra s-1 |
