Summary of study ST001157

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000776. The data can be accessed directly via it's Project DOI: 10.21228/M8CH5C This work is supported by NIH grant, U2C- DK119886.

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Study IDST001157
Study TitleThe gut microbiota plays a central role to modulate the plasma metabolome in response to chronic Angiotensin II infusion (part-I)
Study SummarySix week old C57BL/6 conventional (mice with gut microbiota; conv, n=6) and germ-free (mice without gut microbiota; GF, n=6) mice were infused with angiotensin-II (AngII) for 4 weeks (400ng.kg-1.min-1; Alzet 1004). In parallel control groups, conv (n=6) and GF (n=6) mice received saline via minipumps. Our primary goal was to identify metabolites which were differentially regulated in conventional mice treated with AngII, but not in GF mice, indicating that these metabolites are microbial in origin. Following minipump implantation, animals were housed singly to prevent cross-contamination of microbiota. At the end of fourth week, feces and blood were collected. Both plasma and feces samples were processed and analyzed by using Liquid Chromatography-Tandem Mass Spectroscopy (LC-MS/MS) for metabolite detection (Metabolon).
Institute
Johns Hopkins University
Last NamePluznick
First NameJennifer
Address725 N. Wolfe St., WBSB 205, Baltimore, MD 21205
Emailjpluznick@jhmi.edu
Phone410 614 4660
Submit Date2019-03-20
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2019-05-15
Release Version1
Jennifer Pluznick Jennifer Pluznick
https://dx.doi.org/10.21228/M8CH5C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000776
Project DOI:doi: 10.21228/M8CH5C
Project Title:The gut microbiota plays a central role to modulate the plasma metabolome in response to chronic Angiotensin II infusion
Project Summary:Six week old C57BL/6 conventional (mice with gut microbiota; conv, n=6) and germ-free (mice without gut microbiota; GF, n=6) mice were infused with angiotensin-II (AngII) for 4 weeks (400ng.kg-1.min-1; Alzet 1004). In parallel control groups, conv (n=6) and GF (n=6) mice received saline via minipumps. Our primary goal was to identify metabolites which were differentially regulated in conventional mice treated with AngII, but not in GF mice, indicating that these metabolites are microbial in origin. Following minipump implantation, animals were housed singly to prevent cross-contamination of microbiota. At the end of fourth week, feces and blood were collected. Both plasma and feces samples were processed and analyzed by using Liquid Chromatography-Tandem Mass Spectroscopy (LC-MS/MS) for metabolite detection (Metabolon).
Institute:Johns Hopkins University
Last Name:Pluznick
First Name:Jennifer
Address:725 N. Wolfe St., WBSB 205, Baltimore, MD 21205
Email:jpluznick@jhmi.edu
Phone:410 614 4660

Subject:

Subject ID:SU001222
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male and female

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample TREATMENT
SA080318JHOP-05707Plasma Angiotensin II
SA080319JHOP-05706Plasma Angiotensin II
SA080320JHOP-05708Plasma Angiotensin II
SA080321JHOP-05716Plasma Angiotensin II
SA080322JHOP-05715Plasma Angiotensin II
SA080323JHOP-05705Plasma Angiotensin II
SA080324JHOP-05704Plasma Angiotensin II
SA080325JHOP-05720Plasma Angiotensin II
SA080326JHOP-05719Plasma Angiotensin II
SA080327JHOP-05718Plasma Angiotensin II
SA080328JHOP-05703Plasma Angiotensin II
SA080329JHOP-05717Plasma Angiotensin II
SA080330JHOP-05714Plasma Saline
SA080331JHOP-05713Plasma Saline
SA080332JHOP-05709Plasma Saline
SA080333JHOP-05700Plasma Saline
SA080334JHOP-05699Plasma Saline
SA080335JHOP-05698Plasma Saline
SA080336JHOP-05701Plasma Saline
SA080337JHOP-05702Plasma Saline
SA080338JHOP-05711Plasma Saline
SA080339JHOP-05710Plasma Saline
SA080340JHOP-05697Plasma Saline
SA080341JHOP-05712Plasma Saline
Showing results 1 to 24 of 24

Collection:

Collection ID:CO001216
Collection Summary:Plasma
Collection Protocol Filename:Sample_Collection-Feces_Pluznick.pdf
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR001237
Treatment Summary:Six week old C57BL/6 conventional (mice with gut microbiota; conv, n=6) and germ-free (mice without gut microbiota; GF, n=6) mice were infused with angiotensin-II (AngII) for 4 weeks (400ng.kg-1.min-1; Alzet 1004). In parallel control groups, conv (n=6) and GF (n=6) mice received saline via minipumps.
Treatment Protocol Filename:Treatment_Protocol__Pluznick.pdf

Sample Preparation:

Sampleprep ID:SP001230
Sampleprep Summary:Samples were prepared using the automated MicroLab STARĀ® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. To remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVapĀ® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis.

Combined analysis:

Analysis ID AN001915
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity CSH C18 (100 x 2.1mm, 1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE
Units Peaks area

Chromatography:

Chromatography ID:CH001388
Methods Filename:Ultrahigh_Performance_LC_Method_Pluznick.pdf
Instrument Name:Waters Acquity
Column Name:Waters Acquity CSH C18 (100 x 2.1mm, 1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001771
Analysis ID:AN001915
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:None
Ion Mode:NEGATIVE
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