Summary of study ST001206

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000811. The data can be accessed directly via it's Project DOI: 10.21228/M8VD62 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001206
Study TitleEffects of cold exposure on serum lipidomic in mice
Study SummaryWe aimed to identify lipids released during cold exposure from adipose tissue, with a role in adaptive thermogenesis.
Institute
Joslin Diabetes Center
DepartmentIntegrative Physiology and Metabolism
LaboratoryYu-Hua Tseng lab
Last NameLeiria
First NameLuiz
AddressOne Joslin Place, Boston-MA, USA, 02215
Emailluiz.leiria@joslin.harvard.edu
Phone1 6173091967
Submit Date2019-06-26
Study CommentsJoslin Diabetes Center affiliate of Harvard Medical School
Raw Data AvailableYes
Raw Data File Type(s).wiff
Release Date2019-07-17
Release Version1
Luiz Leiria Luiz Leiria
https://dx.doi.org/10.21228/M8VD62
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000811
Project DOI:doi: 10.21228/M8VD62
Project Title:12-LOX metabolites in cold adaptation
Project Summary:The goal of this project is to understand the role of the enzyme 12-lipoxygenase in the adaptive thermogenesis. We found this enzyme is activated by cold stimulation, then producing lipid metabolites in adipose tissue and releasing them into the circulation to regulate fuel utilisation and thermogenic pathways required for the cold adaptation.
Institute:Joslin Diabetes Center
Department:Integrative Physiology and Metabolism
Laboratory:Yu-Hua Tseng lab
Last Name:Leiria
First Name:Luiz
Address:One Joslin Place, Boston, MA-USA, 02215
Email:luiz.leiri@joslin.harvard.edu
Phone:1 6173091967

Subject:

Subject ID:SU001273
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Temperature Time of exposure
SA084770Control 122C 1 hour
SA084771Control 222C 1 hour
SA084772Control 522C 1 hour
SA084773Control 622C 1 hour
SA084774Control 422C 1 hour
SA084775Control 322C 1 hour
SA084776Thermoneutral 2 female30C 7 days
SA084777Thermoneutral 1 female30C 7 days
SA084778Thermoneutral 3 female30C 7 days
SA084779Thermoneutral 6 female30C 7 days
SA084780Thermoneutral 5 male30C 7 days
SA084781Thermoneutral 5 female30C 7 days
SA084782Thermoneutral 4 female30C 7 days
SA084783Thermoneutral 6 male30C 7 days
SA084784Thermoneutral 4 male30C 7 days
SA084785Thermoneutral 1 male30C 7 days
SA084786Thermoneutral 2 male30C 7 days
SA084787Thermoneutral 3 male30C 7 days
SA0847881-hr cold 15C 1 hour
SA0847891-hr cold 25C 1 hour
SA0847901-hr cold 35C 1 hour
SA0847911-hr cold 45C 1 hour
SA0847921-hr cold 55C 1 hour
SA084793Cold 3 female5C 7 days
SA084794Cold 4 female5C 7 days
SA084795Cold 5 female5C 7 days
SA084796Cold 2 female5C 7 days
SA084797Cold 2 male5C 7 days
SA084798Cold 1 male5C 7 days
SA084799Cold 3 male5C 7 days
SA084800Cold 4 male5C 7 days
SA084801Cold 5 male5C 7 days
SA084802Cold 1 female5C 7 days

Collection:

Collection ID:CO001267
Collection Summary:Mice were anaesthethized with isoflurane, the blood collected through cardiac puncture, and left under room temperature for 30 minutes. After that, the blood was centrifuged for 3 minutes at 14,000 rpm. Then, serum was transferred for a clean 1.5ml microbe and frost.
Sample Type:Blood (serum)
Storage Conditions:-20℃

Treatment:

Treatment ID:TR001288
Treatment Summary:We divided the C57BL6/J mice in 6 groups: females housed in 30C degree for 1 week and females housed in 5C degree for 1 week; Males housed in 30C degree for 1 week and females housed in 5C degree for 1 week; males housed in 22C for 1 hour and males housed in 5C for 1 hour.

Sample Preparation:

Sampleprep ID:SP001281
Sampleprep Summary:Aliquots of 100 µL serum or were taken. A mixture of deuterium-labeled internal standards was added to each aliquot, followed by 3x volume of sample of cold methanol (MeOH). Samples were vortexed for 5 minutes and stored at −20 °C overnight. Cold samples were centrifuged at 14,000g for 10 minutes, and the supernatant was then transferred to a new tube and 3 mL of acidified H2O (pH 3.5) was added to each sample prior to C18 SPE. The methyl formate fractions were collected, dried under nitrogen, and reconstituted in 50 µL MeOH:H2O (1:1, by vol). Samples were transferred to 0.5 mL tubes and centrifuged at 20,000g at 4 °C for 10 minutes. (35ul) of supernatant was transferred to LC–MS/MS vials for analysis using the BERG LC–MS/MS mediator lipidomics platform.

Combined analysis:

Analysis ID AN002008
Analysis type MS
Chromatography type UHPLC
Chromatography system Ekspert MicroLC 200 system
Column Synergi™ Fusion-RP capillary C18 column (150 × 0.5 mm, 4 µm; Phenomenex Inc., Torrance, CA, USA)
MS Type ESI
MS instrument type QTOF
MS instrument name ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE
Units Peak Area

Chromatography:

Chromatography ID:CH001452
Instrument Name:Ekspert MicroLC 200 system
Column Name:Synergi™ Fusion-RP capillary C18 column (150 × 0.5 mm, 4 µm; Phenomenex Inc., Torrance, CA, USA)
Chromatography Type:UHPLC

MS:

MS ID:MS001861
Analysis ID:AN002008
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:MS spectra were acquired in high-resolution mode (>30,000) using a 100-ms accumulation time per spectrum. Full-scan MS/MS was acquired in high sensitivity mode, with an accumulation time optimized per cycle. Collision energy was set using rolling collision energy with a spread of 15V. The identity of a component was confirmed using PeakView® software (SCIEX), and quantification was performed using MultiQuant™ software (SCIEX).
Ion Mode:NEGATIVE