Summary of Study ST001224

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000821. The data can be accessed directly via it's Project DOI: 10.21228/M8JX05 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001224
Study TitleVaginal swab lipidome profiles at 48 h reflect the fat composition of neonatal diet during first two days postnatal
Study TypeMRM-profiling
Study SummaryIn this study, we further investigated the efficacy of using MRM-profiling of vaginal lipids to differentiate PND 2 vaginal swabs between gilts suckled by sow or fed milk replacer. Secondly, we tested the effect of a lard based supplement on vaginal lipid profiles of gilts.
Institute
Purdue University
DepartmentAnimal Sciences
LaboratoryMetabolite Profiling Facility - Purdue University
Last NameFerreira
First NameChristina
Address1203 W. State St, West Lafayette, IN, 47906, USA
Emailcferrei@purdue.edu
Phone7654095924
Submit Date2019-06-06
Num Groupscolostrum suckled (S, n=8); S plus fat supplement (SF, n=5); bottle-fed milk replacer (B, n=8); or B plus fat supplement (BF, n=7
Total Subjects28
Num Females28
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2019-09-23
Release Version1
Christina Ferreira Christina Ferreira
https://dx.doi.org/10.21228/M8JX05
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000821
Project DOI:doi: 10.21228/M8JX05
Project Title:Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet.
Project Type:MRM-profiling
Project Summary:In this study, we further investigated the efficacy of using MRM-profiling of vaginal lipids to differentiate PND 2 vaginal swabs between gilts suckled by sow or fed milk replacer. Secondly, we tested the effect of a lard based supplement on vaginal lipid profiles of gilts.
Institute:Purdue University
Department:Animal Sciences
Laboratory:Metabolite Profiling Facility - Purdue University
Last Name:Ferreira
First Name:Christina
Address:1203 W. State St, West Lafayette, IN, 47906, USA
Email:cferrei@purdue.edu
Phone:7654095924
Funding Source:AgSEED Crossroads funding to support Indiana’s Agriculture and Rural Development
Contributors:KaLynn Harlow, Christina R. Ferreira, Tiago J.P. Sobreira, Theresa Casey, and Kara Stewart

Subject:

Subject ID:SU001291
Subject Type:Mammal
Subject Species:Sus scrofa
Taxonomy ID:9823
Age Or Age Range:2 days after birth
Weight Or Weight Range:above 1.3Kg at birth
Gender:Female
Animal Animal Supplier:Purdue University Animal Sciences Research and Education Swine facility
Animal Housing:Education Swine facility
Animal Water:ad libitum
Animal Inclusion Criteria:Three to four gilts above 1.3Kg at birth were selected per litter from eight different sows which were monitored during parturition

Factors:

Subject type: Mammal; Subject species: Sus scrofa (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Sample Type
SA0868127-R29B serum
SA0868137-R29bB serum
SA0868142-R20B serum
SA0868153-R21B serum
SA0868168-R30B serum
SA0868176-R27B serum
SA0868184-R25bB serum
SA0868194-R25B serum
SA0868205-R26B serum
SA0868211-R18B serum
SA0868228-R30bB serum
SA0868231-R18bB serum
SA086824R27_M1B swab
SA086825R29_M1B swab
SA086826R30_M1B swab
SA086827R18_M1B swab
SA086828R26_M1B swab
SA086829R25_M1B swab
SA086830R21_M1B swab
SA086831R20_M1B swab
SA08683220-MRMR milk replacer
SA08683347-L14-H24bM milk
SA08683447-L14-H24M milk
SA08683544-14S-H24M milk
SA08683619-4N-H24M milk
SA08683728-11Z-H24M milk
SA08683824-14A-H24M milk
SA08683932-4U-H24M milk
SA08684040-4M-H24M milk
SA08684136-1T-H24M milk
SA08684213-Y31S serum
SA08684312-Y29bS serum
SA08684410-Y24S serum
SA08684511-Y27S serum
SA08684612-Y29S serum
SA08684714-Y32S serum
SA08684814-Y32bS serum
SA08684916-Y39S serum
SA0868509-Y23S serum
SA08685115-Y34S serum
SA08685215-Y34bS serum
SA086853Y34_M1S swab
SA086854Y39_M1S swab
SA086855Y32_M1S swab
SA086856Y27_M1S swab
SA086857Y23_M1S swab
SA086858Y24_M1S swab
SA086859Y29_M1S swab
SA086860Y31_M1S swab
Showing results 1 to 49 of 49

Collection:

Collection ID:CO001285
Collection Summary:Blood samples were collected from gilts at 48 h postnatal. Gilts were euthanized approximately 48 h after birth using CO2 inhalation. Following euthanasia, skin of the abdominal and genital regions was cleaned thoroughly using 70% ethanol, and vaginal swabs were taken using a cytology brush (Puritan 2196 Removable Stiff Bristle Tip Brush; QuickMedical; Issaquah, WA) by inserting the tip of the brush into the vulva angled dorsally at 45˚. Once inserted to the base of the bristles, the brush was rotated 360˚ against the vaginal surface. Two consecutive swabs were collected from each animal, and swabs were placed in separate 15 ml sterile polypropylene conical tubes (Falcon™, Fisher Scientific, San Jose, California) and immediately placed on dry ice for transport. Samples were stored in a -80°C freezer until lipid extraction and analysis. Sows were milked during farrowing, and at 24 h after delivery of first piglet. For milk collection piglets were removed from the sow for approximately an hour and then 1 ml oxytocin (VetOne; Boise, ID; 20 USP/ml) was administered IM using a 20g x 1.5-inch needle into the vulva to stimulate milk letdown. Colostrum samples were collected manually from all teats and combined to create a uniform sample. Samples were stored until further analysis at -20°C
Sample Type:Vaginal epithelium
Collection Method:swab
Collection Location:West Lafayette
Collection Frequency:once
Collection Duration:1min
Storage Conditions:-80℃
Collection Vials:Puritan 2196 Removable Stiff Bristle Tip Brush; QuickMedical; Issaquah, WA
Storage Vials:15 ml sterile polypropylene conical tubes (Falcon™, Fisher Scientific, San Jose, California)
Additives:none

Treatment:

Treatment ID:TR001306
Treatment Summary:Three to four gilts were selected per litter from eight different sows which were monitored during parturition. Immediately after delivery, all gilts were towel-dried, weighed, and placed in a holding cart until at least three gilts above 1.3 kg were delivered. Within litter, each gilt was randomly assigned to one of four treatment groups and ear tagged for identification. The four treatment groups were: suckled (S; n=8); suckled plus fat-supplement (SF; n=5); bottle-fed with milk-replacer (B; n=8), and; bottle-fed milk replacer plus fat-supplement (BF; n=7). Body weights were recorded at birth and 48 h. All gilts were administered a 2 ml dose of Camas experimental antibody product (Camas Incorporated; Le Center, MN) using Pump It™ Automatic Delivery System (Genesis Industries, Inc.) at birth, and at 3 h and 9 h after the first dose.

Sample Preparation:

Sampleprep ID:SP001299
Sampleprep Summary:The Bligh & Dyer lipid extraction technique was slightly modified to extract lipids from the vaginal swab samples. A volume of 500 µl distilled water was added to the conical tube containing the swab brushes and vortexed to remove biological material from the brush. The brushes were removed, the sample homogenate was transferred to a new tube, and phase separation was performed by mixing with chloroform/methanol/distilled water (1:2:0.8). Samples were centrifuged, the organic phase (bottom phase) was separated from aqueous phase, divided into four aliquots, and dried in a centrifugal evaporator (Savant SpeedVac AES2010, ThermoFisher Scientific, San Jose, CA). Dried lipid extracts were stored at -20˚C until mass spectrometry analysis. The Bligh & Dyer lipid extraction technique was also used to extract lipids from 48 h serum samples from suckled and bottle-fed piglets; colostrum samples taken from all eight sows (during parturition, 6 h after first piglet born, 12 h after, and 24 h after); and milk replacer used for bottle-feeding. The procedure for these samples began at the phase separation step (i.e. no water was added to the samples).
Processing Storage Conditions:Room temperature
Extraction Method:Bligh & Dyer
Extract Storage:-80℃
Sample Resuspension:acetonitrile/methanol/ammonium acetate 300 mM at 3:6.65:0.35 (v/v)
Sample Derivatization:none
Sample Spiking:none

Combined analysis:

Analysis ID AN002037
Analysis type MS
Chromatography type Unspecified
Chromatography system Agilent 6410
Column none
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410 QQQ
Ion Mode UNSPECIFIED
Units ion counts

Chromatography:

Chromatography ID:CH001477
Chromatography Summary:Direct infusion
Instrument Name:Agilent 6410
Column Name:none
Chromatography Type:Unspecified

MS:

MS ID:MS001889
Analysis ID:AN002037
Instrument Name:Agilent 6410 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Pooled sample injections (8 µl) were delivered to the micro-autosampler (G1377A) in a QQQ6410 triple quadrupole mass spectrometer (Agilent Technologies, San Jose, CA) equipped with an ESI ion source. The solvent pumped between injections (CapPump G1376A, Agilent Technologies, San Jose, CA) was acetonitrile with 1% formic acid at 10µL/min. Between sample injections, methanol/chloroform was injected to remove any remaining lipids before the next sample injection
Ion Mode:UNSPECIFIED
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