Summary of study ST001225

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000822. The data can be accessed directly via it's Project DOI: 10.21228/M8F69D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001225
Study TitleAquamin and Prevention of Colon Cancer (part-I)
Study TypeMS analysis
Study SummaryWe propose to evaluate microbial and metabolic profiles in baseline and endpoint colonic mucosal, fecal, and serum samples from human patients at risk for CRC and enrolled in a 90-day phase I clinical trial. Patients will receive daily supplementation with calcium alone, a calcium-rich multimineral (Aquamin?), or placebo (maltodextrin) (n=10 per group). We hypothesize that dietary supplementation will correlate with CRC-protective metabolic profiles and that multimineral supplementation will generate more favorable profiles than calcium supplementation alone.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
AddressAnn Arbor, MI
Emailmkachman@med.umich.edu
Phone734-232-0842
Submit Date2019-07-23
Num Groups24
Total Subjects18
Study CommentsColorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer related death when both genders are combined. Epidemiologically, calcium intake has been protective against colonic adenomas and even colon cancer. Calcium supplementation has reduced the risk of colon adenoma formation in subjects with a history of previous colon polyps. The utility of calcium supplementation for colon cancer prevention is somewhat modulated by the modest or inconsistent level of protection afforded. Our preliminary data in mice and human enterocyte models shows that dietary supplementation with a multimineral supplement (Aquamin?) containing calcium in combination with 72 measureable trace minerals is more protective against tumors and epithelial growth dysregulation than calcium alone. One potential mechanism, supported by our rodent data, is that multimineral supplementation alters gut microbial populations to generate bile acid and short chain fatty acid (SCFA) profiles that are CRC-protective.
Raw Data AvailableYes
Raw Data File Type(s).m
Analysis Type DetailLC-MS
Release Date2019-09-23
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8F69D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000822
Project DOI:doi: 10.21228/M8F69D
Project Title:Aquamin and Prevention of Colon Cancer
Project Type:MS analysis
Project Summary:Effects of calcium and multi-mineral supplementation on gut microbes and microbially-derived metabolites in patients at risk for colon cancer
Institute:University of Michigan
Department:Pathology
Laboratory:Aslam Lab
Last Name:Aslam
First Name:Muhammad Nadeem
Address:Ann Arbor, MI
Email:mnaslam@umich.edu
Phone:734-936-1897

Subject:

Subject ID:SU001292
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Time Point
SA086861S00031741Baseline
SA086862S00031739Baseline
SA086863S00031738Baseline
SA086864S00031742Baseline
SA086865S00031743Baseline
SA086866S00031727Baseline
SA086867S00031744Baseline
SA086868S00031737Baseline
SA086869S00031740Baseline
SA086870S00031731Baseline
SA086871S00031730Baseline
SA086872S00031736Baseline
SA086873S00031728Baseline
SA086874S00031732Baseline
SA086875S00031729Baseline
SA086876S00031733Baseline
SA086877S00031734Baseline
SA086878S00031735Baseline
SA086879S00031758Day 90
SA086880S00031756Day 90
SA086881S00031757Day 90
SA086882S00031759Day 90
SA086883S00031761Day 90
SA086884S00031755Day 90
SA086885S00031762Day 90
SA086886S00031760Day 90
SA086887S00031745Day 90
SA086888S00031748Day 90
SA086889S00031747Day 90
SA086890S00031746Day 90
SA086891S00031749Day 90
SA086892S00031750Day 90
SA086893S00031753Day 90
SA086894S00031752Day 90
SA086895S00031751Day 90
SA086896S00031754Day 90
Showing results 1 to 36 of 36

Collection:

Collection ID:CO001286
Collection Summary:Colonic mucosal tissue
Sample Type:Colon

Treatment:

Treatment ID:TR001307
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP001300
Sampleprep Summary:Two-step solvent extraction. Supernatants are combined, dried, and re-suspended for LCMS separation by RPLC and measurements by ESI- QQQ MRM methods. Analytes are reported as uM, and CV's are generally <15%.
Sampleprep Protocol Filename:Bile_acids_analysis.doc

Combined analysis:

Analysis ID AN002038
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1260
Column Waters Acquity BEH C18 (50 x 2mm, 1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode POSITIVE
Units pmol/mg

Chromatography:

Chromatography ID:CH001478
Methods Filename:2014-07-30-Bile_acid-full-BEH-C18-I.m
Instrument Name:Agilent 1260
Column Name:Waters Acquity BEH C18 (50 x 2mm, 1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001890
Analysis ID:AN002038
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:NA
Ion Mode:POSITIVE
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