Summary of study ST001291

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000873. The data can be accessed directly via it's Project DOI: 10.21228/M8V393 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST001291
Study TitleMetabolomic study of disease progression in scrapie prion infected (RML) mice
Study TypeTimecourse experiment
Study SummaryWe used NMR spectroscopy to investigate metabolic perturbations in the brains of RML infected mice along the progression of prion disease at 30, 60, 90, 120, and 125 days post-inoculation.
Institute
University of Alberta
DepartmentBiochemistry
Last NameFu
First NameZelin
Address116 St & 85 Ave, Edmonton, AB T6G 2R3
Emailzelin2@ualberta.ca
Phone780-492-3006
Submit Date2019-12-16
Raw Data AvailableYes
Raw Data File Type(s).cnx
Analysis Type DetailNMR
Release Date2020-01-13
Release Version1
Zelin Fu Zelin Fu
https://dx.doi.org/10.21228/M8V393
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000873
Project DOI:doi: 10.21228/M8V393
Project Title:Metabolomic study of disease progression in scrapie prion infected mice
Project Type:NMR quantitative analysis
Project Summary:NMR spectroscopy on brain extracts from scrapie prion infected mice
Institute:University of Alberta
Department:Biochemistry
Laboratory:Brian D. Sykes
Last Name:Fu
First Name:Zelin
Address:116 St & 85 Ave Edmonton AB T6G 2R3
Email:zelin2@ualberta.ca
Phone:780-492-3006

Subject:

Subject ID:SU001364
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Inocula Timepoint
SA09379782806NBH 120 dpi
SA09379882807NBH 120 dpi
SA09379982808NBH 120 dpi
SA09380082805NBH 120 dpi
SA09380182592NBH 120 dpi
SA09380282576NBH 120 dpi
SA09380382595NBH 120 dpi
SA09380482574NBH 120 dpi
SA09380582586NBH 150 dpi
SA09380682577NBH 150 dpi
SA09380782646NBH 150 dpi
SA09380882587NBH 150 dpi
SA09380982863NBH 150 dpi
SA09381082864NBH 150 dpi
SA09381182624NBH 150 dpi
SA09381282861NBH 150 dpi
SA09381382621NBH 150 dpi
SA09381482588NBH 150 dpi
SA09381582589NBH 150 dpi
SA09381682608NBH 30 dpi
SA09381782591NBH 30 dpi
SA09381882650NBH 30 dpi
SA09381982660NBH 30 dpi
SA09382082590NBH 30 dpi
SA09382182659NBH 30 dpi
SA09382282607NBH 30 dpi
SA09382382656NBH 60 dpi
SA09382482579NBH 60 dpi
SA09382582655NBH 60 dpi
SA09382682581NBH 60 dpi
SA09382782580NBH 60 dpi
SA09382882657NBH 60 dpi
SA09382982583NBH 90 dpi
SA09383082658NBH 90 dpi
SA09383182598NBH 90 dpi
SA09383282597NBH 90 dpi
SA09383382584NBH 90 dpi
SA09383482582NBH 90 dpi
SA09383582585NBH 90 dpi
SA09383682644RML 120 dpi
SA09383782615RML 120 dpi
SA09383882616RML 120 dpi
SA09383982642RML 120 dpi
SA09384082617RML 120 dpi
SA09384182634RML 120 dpi
SA09384282635RML 120 dpi
SA09384382641RML 120 dpi
SA09384482661RML 150 dpi
SA09384582662RML 150 dpi
SA09384682613RML 150 dpi
SA09384782665RML 150 dpi
SA09384882612RML 150 dpi
SA09384982664RML 150 dpi
SA09385082663RML 150 dpi
SA09385182625RML 30 dpi
SA09385282652RML 30 dpi
SA09385382653RML 30 dpi
SA09385482611RML 30 dpi
SA09385582610RML 30 dpi
SA09385682609RML 30 dpi
SA09385782636RML 60 dpi
SA09385882630RML 60 dpi
SA09385982637RML 60 dpi
SA09386082628RML 60 dpi
SA09386182629RML 60 dpi
SA09386282638RML 60 dpi
SA09386382640RML 90 dpi
SA09386482633RML 90 dpi
SA09386582639RML 90 dpi
SA09386682632RML 90 dpi
SA09386782631RML 90 dpi
SA09386882619RML 90 dpi
SA09386982618RML 90 dpi
Showing results 1 to 73 of 73

Collection:

Collection ID:CO001359
Collection Summary:Brains were harvested and fash-frozen with dry ice
Sample Type:Brain

Treatment:

Treatment ID:TR001379
Treatment Summary:Wild type FVB mice were subjected to intracerebral injection to be inoculated with 30ul of 1% (w.v) RML brain homogenate or normal brain homogenate. At 30, 60, 90, 120 and 150 dpi, a sub-set of RML and NBH inoculated animals were euthanized.

Sample Preparation:

Sampleprep ID:SP001372
Sampleprep Summary:Half brains were homogenized with Dounce tissue homogenizers to make 10% (w/v) homogenate. The homogenate is then subject to ultracentrifuge at 100,000xg for 1h at 4°C, the resulting supernatant was neutralized with Tris buffer (0.5M, pH6.8) one tenth the volume of the supernatant. Then the neutralized supernatant is passed through 3kDa molecular weight cut-off filters at 4000 rpm for 45 mins at 4°C. Filtered supernatant was tried at SpeedVac Vacuum Concentrator at low heating until try pellets were received. Try pellets were then dissolved with 530ul of water and 60 ul of D2O containing 5mM of DSS. A final volume of 600ul for each sample was achieved.

Analysis:

Analysis ID:AN002146
Analysis Type:NMR
Num Factors:10
Num Metabolites:50
Units:uM

NMR:

NMR ID:NM000156
Analysis ID:AN002146
Instrument Name:INOVA NMR spectrometer
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:500MHz
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