Summary of Study ST001327

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000905. The data can be accessed directly via it's Project DOI: 10.21228/M8Q69W This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001327
Study TitleMitochondrial Lipid Profiles in Traumatic Optic Neuropathy
Study SummaryC57Bl/6J Mice were exposed to sonication to induce a traumatic optic neuropathy model. Optic nerves were harvested and mitochondrial isolation was performed. Lipids were then extracted from isolated mitochondria. Mass spectrometry was used to analyze mitochondrial lipids.
Institute
University of Miami
DepartmentOphthalmology, Bascom Palmer Eye Institute
Last NameBhattacharya
First NameSanjoy K
Address1638 NW 10th Avenue, Suite 707A University of Miami Miami, FL, 33136
EmailSBhattacharya@med.miami.edu
Phone305-482-4103
Submit Date2020-03-09
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2020-03-26
Release Version1
Sanjoy K Bhattacharya Sanjoy K Bhattacharya
https://dx.doi.org/10.21228/M8Q69W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000905
Project DOI:doi: 10.21228/M8Q69W
Project Title:Mitochondrial Lipid Profiles in Traumatic Optic Neuropathy
Project Summary:C57Bl/6J Mice were exposed to sonication to induce a traumatic optic neuropathy model. Optic nerves were harvested and mitochondrial isolation was performed. Lipids were then extracted from isolated mitochondria. Mass spectrometry was used to analyze mitochondrial lipids.
Institute:University of Miami
Department:Bascom Palmer Eye Institute
Last Name:Bhattacharya
First Name:Sanjoy K
Address:1638 NW 10th Avenue, Suite 707A University of Miami Miami, FL, 33136
Email:SBhattacharya@med.miami.edu
Phone:305-482-4103

Subject:

Subject ID:SU001401
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:2 Months
Gender:Male and female

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Exposure Days Post Exposure Sex
SA096333C7No Exposure Control F
SA096334C8No Exposure Control F
SA096335C6No Exposure Control F
SA096336C5No Exposure Control F
SA096337C1No Exposure Control M
SA096338C2No Exposure Control M
SA096339C3No Exposure Control M
SA096340C4No Exposure Control M
SA096341D14_2Sonication Day Fourteen F
SA096342D14_1Sonication Day Fourteen F
SA096343D14_3Sonication Day Fourteen F
SA096344D14_4Sonication Day Fourteen F
SA096345D14_8Sonication Day Fourteen M
SA096346D14_6Sonication Day Fourteen M
SA096347D14_5Sonication Day Fourteen M
SA096348D14_7Sonication Day Fourteen M
SA096349D1_5Sonication Day One F
SA096350D1_8Sonication Day One F
SA096351D1_6Sonication Day One F
SA096352D1_7Sonication Day One F
SA096353D1_1Sonication Day One M
SA096354D1_2Sonication Day One M
SA096355D1_4Sonication Day One M
SA096356D1_3Sonication Day One M
SA096357D7_1Sonication Day Seven F
SA096358D7_3Sonication Day Seven F
SA096359D7_2Sonication Day Seven F
SA096360D7_4Sonication Day Seven F
SA096361D7_5Sonication Day Seven M
SA096362D7_7Sonication Day Seven M
SA096363D7_6Sonication Day Seven M
SA096364D7_8Sonication Day Seven M
Showing results 1 to 32 of 32

Collection:

Collection ID:CO001396
Collection Summary:2 month old mice were exposed to sonication-induced trauma. Optic nerves were dissected from optic nerve head to optic chiasm.
Sample Type:Neurons

Treatment:

Treatment ID:TR001416
Treatment Summary:4 groups of 8 mice, 4 males and 4 females per group. Control group was euthanized at 2 months. Other three groups were exposed to sonication to induce traumatic optic neuropathy. Then groups were euthanized at one day, seven days, and fourteen days, respectively.

Sample Preparation:

Sampleprep ID:SP001409
Sampleprep Summary:Samples were homogenized and mitochondria were isolated. Lipids were extracted using butanol/methanol lipid extraction and run through mass spectrometer.

Combined analysis:

Analysis ID AN002209 AN002210
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Accela 600 Thermo Accela 600
Column Thermo Acclaim C18 (150 x 2.1mm,3m) Thermo Acclaim C18 (150 x 2.1mm,3m)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE POSITIVE
Units Main area Main area

Chromatography:

Chromatography ID:CH001620
Instrument Name:Thermo Accela 600
Column Name:Thermo Acclaim C18 (150 x 2.1mm,3m)
Chromatography Type:Reversed phase

MS:

MS ID:MS002055
Analysis ID:AN002209
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI operated at a spray voltage of 4.4 kV, capillary temperature of 329.60 Celsius, sheath gas flow rate of 45.30, and an auxillary gas flow of 14.73. The scan range was set at 150-1500 m/z.
Ion Mode:NEGATIVE
  
MS ID:MS002056
Analysis ID:AN002210
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI operated at a spray voltage of 4.4 kV, capillary temperature of 329.60 Celsius, sheath gas flow rate of 45.30, and an auxillary gas flow of 14.73. The scan range was set at 150-1500 m/z.
Ion Mode:POSITIVE
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