Summary of study ST001435

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000986. The data can be accessed directly via it's Project DOI: 10.21228/M87Q4T This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001435
Study TitleMaple Sap analysis from Ontario Canada
Study SummarySap samples from sugar maple trees across the Canadian province of Ontario were collected in 2019. These samples were minimally prepared and analyzed in both positive ESI and negative ESI by C18 and HILIC chromatography. This was done to uncover the chemical changes that occurred in the sap over the season. This will serve as the base for future analysis of maple syrup where compounds that may be responsible for specific organoleptic properties can be linked back to precursors found here in the sap.
Agriculture and Agri-Food Canada
Last NameRenaud
First NameJustin
Address1391 Sandford Street, London, Ontario, LN5V 4T3, Canada
Submit Date2020-04-17
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2020-07-29
Release Version1
Justin Renaud Justin Renaud application/zip

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Project ID:PR000986
Project DOI:doi: 10.21228/M87Q4T
Project Title:Metabolomics reveals plant and microbial driven chemical evolution in Acer saccharum sap over a maple syrup production season.
Project Type:Non-targeted high resolution MS of Tree sap over a Maple syrup production season
Project Summary:This project seeks to understand the chemicals present in the Sap of sugar maple trees that act as precursors to the flavor compounds produced.
Institute:Agriculture and Agri-Food Canada
Last Name:Renaud
First Name:Justin
Address:1391 Sandford Street, London, Ontario, LN5V 4T3, Canada
Funding Source:MITACS - Ontario Maple Syrup Producer Association


Subject ID:SU001509
Subject Type:Plant
Subject Species:Acer Saccharum
Taxonomy ID:4024


Subject type: Plant; Subject species: Acer Saccharum (Factor headings shown in green)

mb_sample_id local_sample_id OMSPA region
SA121902129_posAlgoma & District
SA121903118_posAlgoma & District
SA121904134_posAlgoma & District
SA121905140_posAlgoma & District
SA121906141_posAlgoma & District
SA121907120_posAlgoma & District
SA121908139_posAlgoma & District
SA121909125_posAlgoma & District
SA121910147_posAlgoquin & District
SA121911159_posAlgoquin & District
SA121912160_posAlgoquin & District
SA12191963_posGrey Bruce & District
SA12192059_posGrey Bruce & District
SA12192140_posGrey Bruce & District
SA12192243_posGrey Bruce & District
SA12192346_posGrey Bruce & District
SA12192439_posGrey Bruce & District
SA12192536_posGrey Bruce & District
SA12192629_posGrey Bruce & District
SA12192730_posGrey Bruce & District
SA12192835_posGrey Bruce & District
SA12192947_posGrey Bruce & District
SA12193052_posGrey Bruce & District
SA12193157_posGrey Bruce & District
SA121932182_posHaliburton & Kawartha
SA121933183_posHaliburton & Kawartha
SA121934184_posHaliburton & Kawartha
SA121935181_posHaliburton & Kawartha
SA121936180_posHaliburton & Kawartha
SA121937172_posHaliburton & Kawartha
SA121938178_posHaliburton & Kawartha
SA121939179_posHaliburton & Kawartha
SA121940176_posHaliburton & Kawartha
SA121941258_posLanark & District
SA121942255_posLanark & District
SA121943248_posLanark & District
SA121944259_posLanark & District
SA121945251_posLanark & District
SA121946265_posLanark & District
SA121947264_posLanark & District
SA121948263_posLanark & District
SA121949211_posOttawa Valley District
SA121950212_posOttawa Valley District
SA121951210_posOttawa Valley District
SA121952200_posOttawa Valley District
SA121953213_posOttawa Valley District
SA121954204_posOttawa Valley District
SA121955205_posOttawa Valley District
SA121956219_posOttawa Valley District
SA121957220_posOttawa Valley District
SA121958221_posOttawa Valley District
SA121959199_posOttawa Valley District
SA121960218_posOttawa Valley District
SA121961215_posOttawa Valley District
SA121962217_posOttawa Valley District
SA121963214_posOttawa Valley District
SA121964216_posOttawa Valley District
SA121965198_posOttawa Valley District
SA121966194_posOttawa Valley District
SA121967236_posQuinte & District
SA121968237_posQuinte & District
SA121969229_posQuinte & District
SA121970227_posQuinte & District
SA121971233_posQuinte & District
SA121972241_posQuinte & District
SA121973244_posQuinte & District
SA121974245_posQuinte & District
SA121975243_posQuinte & District
SA121976242_posQuinte & District
SA121977224_posQuinte & District
SA121978240_posQuinte & District
SA121979238_posQuinte & District
SA121980105_posSimcoe & District
SA121981104_posSimcoe & District
SA121982103_posSimcoe & District
SA121983106_posSimcoe & District
SA121984107_posSimcoe & District
SA121985109_posSimcoe & District
SA121986108_posSimcoe & District
SA12198798_posSimcoe & District
SA121988102_posSimcoe & District
SA121989110_posSimcoe & District
SA12199090_posSimcoe & District
SA12199194_posSimcoe & District
SA12199282_posSimcoe & District
SA12199385_posSimcoe & District
SA12199417_posSouthwestern Ontario
SA12199521_posSouthwestern Ontario
SA12199623_posSouthwestern Ontario
SA12199713_posSouthwestern Ontario
SA1219985_posSouthwestern Ontario
SA1219996_posSouthwestern Ontario
SA12200010_posSouthwestern Ontario
SA12200122_posSouthwestern Ontario
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Collection ID:CO001504
Collection Summary:Sap samples (n = 282) were collected as approximately 10 ml aliquots into 15 ml plastic centrifuge tubes contain 1.5 mg of sodium benzoate and stored at 4 ºC. Samples were diluted 1:3 in methanol containing 0.15% formic acid and 30 mM ammonium formate
Sample Type:Plant
Storage Conditions:4℃


Treatment ID:TR001524
Treatment Summary:In 2019, maple sap and syrup samples were collected over the entire production season by 12 members of the Ontario Maple Syrup Producers Association (OMSPA). Sap samples were collected into tubes containing 1.5 mg sodium benzoate.

Sample Preparation:

Sampleprep ID:SP001517
Sampleprep Summary:Samples were diluted 1:3 with methanol containing 0.15% FA and 30mM ammonium formate

Combined analysis:

Analysis ID AN002398
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1290
Column Agilent HILIC-Z (2.1 × 100 mm, 2.7 µm)
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Units concentrations (ug/ml)


Chromatography ID:CH001762
Instrument Name:Agilent 1290
Column Name:Agilent HILIC-Z (2.1 × 100 mm, 2.7 µm)
Column Temperature:35
Flow Gradient:0 min, 100% B; 0.5 min, 100% B; 5.3 min, 80% B; 9.5 min, 30% B; 13.5 min, 30% B, 14.5 min 100% B and 16.5 min, 100% B.
Flow Rate:0.350uL min01
Solvent A:20 mM ammonium formate in water
Solvent B:20 mM ammonium formate in 90% acetonitrile
Chromatography Type:HILIC


MS ID:MS002239
Analysis ID:AN002398
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Comments:The HILIC acquisition mode was a top 3 data-dependent acquisition (DDA) experiment comprised of a full MS scan in the mass range of m/z 58-870 at 35,000 resolution, followed by MS/MS scans at 17,500 resolution, isolation window of m/z 1.2 and collision energy of 28