Summary of Study ST001508
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001017. The data can be accessed directly via it's Project DOI: 10.21228/M87M4S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001508 |
Study Title | Liver metabolomics in old liver-specific SIRT6 overexpressing mice |
Study Summary | Our previous data had suggested that gluconeogenesis capacity from the precursors lactate and glycerol declines in old C57BL/6 mice. This decline was rescued by whole body SIRT6 overexpression, but not in liver-specific SIRT6 overexpression. Here we preformed liver metabolomics in old liver-specific SIRT6 overexpression and control mice. |
Institute | Bar Ilan University |
Department | The Mina & Everard Goodman Faculty of Life Sciences |
Laboratory | Laboratory of Prof. Haim Cohen |
Last Name | Cohen |
First Name | Chaim |
Address | Bar Ilan University, Ramat Gan, Israel, 5290002 |
Haim.Cohen@biu.ac.il | |
Phone | +97235318383 |
Submit Date | 2020-10-07 |
Num Groups | 4 |
Total Subjects | 20 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-03-11 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR001017 |
Project DOI: | doi: 10.21228/M87M4S |
Project Title: | Hepatic [U-13C]Lactate tracing and metabolomics in young and old WT and SIRT6 overexpressing mice |
Project Type: | MS analysis |
Project Summary: | Our previous data had suggested that gluconeogenesis capacity from the precursors lactate and glycerol declines in old C57BL/6 mice. This decline was rescued by whole body SIRT6 overexpression. Since the liver is the main gluconeogenic organ, we performed here liver metabolomics in young (6 months) versus old (20-24 months) WT and SIRT6-transgenic mice. We used liver tissues of 6h morning-fasted mice, either in naïve state or 15 minutes after intraperitoneal injection of [U-13C]Lactate. |
Institute: | Bar Ilan University |
Department: | The Mina & Everard Goodman Faculty of Life Sciences |
Laboratory: | Laboratory of Prof. Haim Cohen |
Last Name: | Cohen |
First Name: | Chaim |
Address: | Ramat Gan, Ramat Gan, Israel, 5290002, Israel |
Email: | Haim.Cohen@biu.ac.il |
Phone: | +97235318383 |
Funding Source: | Israel Science Foundation (621/13 and 777/16), I-Core foundation (1775/12) and (41/11) and Laura and Isaac Perlmutter Foundation |
Contributors: | Asael Roichman, Ifat Abramovich, Eyal Gottlieb, Haim Yosef Cohen |
Subject:
Subject ID: | SU001582 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6JOlaHsd |
Age Or Age Range: | 23-25 months |
Gender: | Male |
Animal Animal Supplier: | Envigo |
Animal Housing: | Animal rooms were maintained at 21°C-24°C and 35%-75% relative humidity |
Animal Light Cycle: | 12/12-h (7am to 7pm) dark-light cycle |
Animal Feed: | Altromin 1324 (chow) |
Animal Water: | Ad lib |
Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Genotype |
---|---|---|
SA126793 | 07_Liver_CRE | Albumin Cre control |
SA126794 | 08_Liver_CRE | Albumin Cre control |
SA126795 | 16_Liver_CRE | Albumin Cre control |
SA126796 | 04_Liver_CRE | Albumin Cre control |
SA126797 | 19_Liver_CRE | Albumin Cre control |
SA126798 | 12_Liver_KISS | Flox control |
SA126799 | 14_Liver_KISS | Flox control |
SA126800 | 11_Liver_KISS | Flox control |
SA126801 | 02_Liver_KISS | Flox control |
SA126802 | 06_Liver_KISS | Flox control |
SA126803 | 10_Liver_OE | Liver specific SIRT6 overexpression |
SA126804 | 03_Liver_OE | Liver specific SIRT6 overexpression |
SA126805 | 18_Liver_OE | Liver specific SIRT6 overexpression |
SA126806 | 01_Liver_OE | Liver specific SIRT6 overexpression |
SA126807 | 17_Liver_OE | Liver specific SIRT6 overexpression |
SA126808 | 20_Liver_WT | Wild-type |
SA126809 | 15_Liver_WT | Wild-type |
SA126810 | 09_Liver_WT | Wild-type |
SA126811 | 13_Liver_WT | Wild-type |
SA126812 | 05_Liver_WT | Wild-type |
Showing results 1 to 20 of 20 |
Collection:
Collection ID: | CO001577 |
Collection Summary: | Mice were euthanized by cervical dislocation, left liver lobe was collected as fast as possible (<30 sec) and immediately flash-frozen in liquid nitrogen. Tissues were stored at -80C until metabolite extraction. |
Sample Type: | Liver |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001597 |
Treatment Summary: | All mice were fasted for 6h (8AM-2PM). Tail blood was collected from all mice for less than 2 minutes, followed by mouse sacrifice. |
Animal Fasting: | 6h morning fast |
Animal Endp Euthanasia: | Cervical dislocation |
Sample Preparation:
Sampleprep ID: | SP001590 |
Sampleprep Summary: | Frozen liver pieces weighting ~30mg were transferred into soft tissue homogenizing CK 14 tubes containing 1.4 mm ceramic beads (Bertin corp.) prefilled with 1 ml of cold (-20°C) metabolites extraction solvent (Methanol: Acetonitrile:H2O, 50:30:20) and kept on ice. Samples were homogenized using precellys 24 tissue homogenizer (3X20sec at 6000 rpm, with a 30sec gap between each of the three cycles, Bertin Technologies) cooled to 2°C. Homogenized extracts were centrifuged in the Precellys tubes at 18,000g for 15 minutes at 4°C, supernatants were collected in microcentrifuge tubes and centrifuged again at 18,000g for 10 minutes at 4°C. The supernatants were transferred to glass HPLC vials and kept at -75 °C prior to LC-MS analysis. |
Extract Storage: | -80℃ |
Combined analysis:
Analysis ID | AN002499 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | Peak Area |
Chromatography:
Chromatography ID: | CH001828 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
Column Temperature: | 45 |
Flow Rate: | 0.2 ml/min |
Solvent A: | 100% water; 20 mM ammonium carbonate adjusted to pH 9.2; 0.1% of 25% ammonium hydroxide |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |
MS:
MS ID: | MS002319 |
Analysis ID: | AN002499 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | polarity switching mode to enable both positive and negative ions was used |
Ion Mode: | UNSPECIFIED |