Summary of study ST001644

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001052. The data can be accessed directly via it's Project DOI: 10.21228/M8QD76 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001644
Study TitleIn Vitro Characterization and Metabolomic Analysis of Cold-Stored Platelets
Study SummaryPlatelet concentrates are currently stored at room temperature (RPs) under constant agitation for up to 5-7 days depending on national regulations. However, platelet quality deteriorates during storage and room temperature storage also increases the risk of bacterial growth. Previous studies have shown that cold-stored platelets (CPs) have higher hemostatic function and can be stored for up to three weeks. While these studies have compared the metabolic phenotypes of CPs and RPs, they have not compared the impact of storage temperature and cold agitation (CPAs) on platelet function, nor have they identified metabolic correlates to such parameters. In vitro analysis showed CPAs and CPs had reduced count, faster CD62P expression and increased lactadherin binding. Furthermore, CPAs and CPs had higher maximal aggregation and a reduced aggregation lag phase compared to RPs. Metabolomic analysis revealed CPAs and CPs exhibited lower oxidative stress shown by preserved glutathione and pentose phosphate pools. CPAs and CPs also had reduced markers of beta-oxidation and amino acid catabolism demonstrating reduced needs for energy. Agitation did not significantly impact in vitro function or metabolomic parameters of cold-stored platelets. Correlation of in vitro and metabolomic results highlighted important metabolites that may contribute to stored platelet functions.
Institute
University of Colorado Anschutz Medical Campus
DepartmentBiochemistry and Molecular Genetics
LaboratoryAngelo D'Alessandro
Last NameD'Alessandro
First NameAngelo
Address12801 E 17th Ave L18-9403D
Emailangelo.dalessandro@cuanschutz.edu
Phone3037245798
Submit Date2021-01-08
Num Groups3
Total Subjects8
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2021-01-25
Release Version1
Angelo D'Alessandro Angelo D'Alessandro
https://dx.doi.org/10.21228/M8QD76
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001052
Project DOI:doi: 10.21228/M8QD76
Project Title:In Vitro Characterization and Metabolomic Analysis of Cold-Stored Platelets
Project Summary:Platelet concentrates are currently stored at room temperature (RPs) under constant agitation for up to 5-7 days depending on national regulations. However, platelet quality deteriorates during storage and room temperature storage also increases the risk of bacterial growth. Previous studies have shown that cold-stored platelets (CPs) have higher hemostatic function and can be stored for up to three weeks. While these studies have compared the metabolic phenotypes of CPs and RPs, they have not compared the impact of storage temperature and cold agitation (CPAs) on platelet function, nor have they identified metabolic correlates to such parameters. In vitro analysis showed CPAs and CPs had reduced count, faster CD62P expression and increased lactadherin binding. Furthermore, CPAs and CPs had higher maximal aggregation and a reduced aggregation lag phase compared to RPs. Metabolomic analysis revealed CPAs and CPs exhibited lower oxidative stress shown by preserved glutathione and pentose phosphate pools. CPAs and CPs also had reduced markers of beta-oxidation and amino acid catabolism demonstrating reduced needs for energy. Agitation did not significantly impact in vitro function or metabolomic parameters of cold-stored platelets. Correlation of in vitro and metabolomic results highlighted important metabolites that may contribute to stored platelet functions.
Institute:University of Colorado Anschutz Medical Campus
Department:Biochemistry and Molecular Genetics
Laboratory:Angelo D'Alessandro
Last Name:D'Alessandro
First Name:Angelo
Address:12801 E 17th Ave L18-9403D, Aurora, Colorado, 80045, USA
Email:angelo.dalessandro@cuanschutz.edu
Phone:303-724-5798
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