Summary of Study ST001968

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001253. The data can be accessed directly via it's Project DOI: 10.21228/M8R98V This work is supported by NIH grant, U2C- DK119886.

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Study IDST001968
Study TitleAnalytical methodology for a metabolome atlas of goat’s plasma, milk and feces using 1H-NMR and UHPLC-HRMS:MS/plasma
Study SummaryMetabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factor that can also alter animal integrity and welfare. Some studies have al-ready investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (Nu-clear Magnetic Resonance or Mass Spectrometry). Only few studies with multi-platform ap-proaches are also used with a single or a multi type of sample, but they mainly consider dairy cows metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabol-ic atlas of goat plasma, milk and feces, based on healthy animals. Our study describes a Standard Operating Procedure for three goat matrices: blood plasma, milk, and feces using multiple plat-forms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample prep-aration procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.
Institute
INSERM
Last NameMartias
First NameCecile
Address10 Boulevard tonnelé, Tours, Indre et Loire, 37000, France
Emailcecile.martias@univ-tours.fr
Phone0247366351
Submit Date2021-09-27
Analysis Type DetailLC-MS
Release Date2022-01-02
Release Version1
Cecile Martias Cecile Martias
https://dx.doi.org/10.21228/M8R98V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001253
Project DOI:doi: 10.21228/M8R98V
Project Title:Analytical methodology for a metabolome atlas of goat’s plasma, milk and feces using 1H-NMR and UHPLC-HRMS
Project Summary:Metabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factors that can also alter animal integrity and welfare. Some studies have already investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (Nuclear Magnetic Resonance or Mass Spectrometry). Only few studies with multi-platform approaches are also used with a single or a multi type of sample, but they mainly consider dairy cows metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabolic atlas of goat plasma, milk, and feces, based on healthy animals. Our study describes a Standard Operating Procedure for three goat matrices: blood plasma, milk, and feces using multiple platforms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample preparation procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.
Institute:INSERM
Department:INSERM
Last Name:Martias
First Name:Cecile
Address:10 Boulevard tonneléTours, Indre et Loire, 37000, France
Email:cecile.martias@univ-tours.fr
Phone:0247366351

Subject:

Subject ID:SU002048
Subject Type:Mammal
Subject Species:Capra hircus

Factors:

Subject type: Mammal; Subject species: Capra hircus (Factor headings shown in green)

mb_sample_id local_sample_id Protocole
SA184969P_200_P1_9P1
SA184970P_200_P1_10P1
SA184971P_200_P1_8P1
SA184972P_200_P1_1P1
SA184973P_200_P1_7P1
SA184974P_200_P1_2P1
SA184975P_200_P1_3P1
SA184976P_200_P1_4P1
SA184977P_200_P1_6P1
SA184978P_200_P1_5P1
SA184997P_200_P2_9p2
SA184998P_200_P2_10p2
SA184979P_200_P2_7P2
SA184980P_200_P2_8P2
SA184981P_200_P2_5P2
SA184982P_200_P2_6P2
SA184983P_200_P2_4P2
SA184984P_200_P2_1P2
SA184985P_200_P2_2P2
SA184986P_200_P2_3P2
SA184987P_200_P4_7P4
SA184988P_200_P4_8P4
SA184989P_200_P4_10P4
SA184990P_200_P4_6P4
SA184991P_200_P4_9P4
SA184992P_200_P4_3P4
SA184993P_200_P4_1P4
SA184994P_200_P4_5P4
SA184995P_200_P4_2P4
SA184996P_200_P4_4P4
Showing results 1 to 30 of 30

Collection:

Collection ID:CO002041
Collection Summary:-
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR002060
Treatment Summary:P1 : Blood:MeOH:CHCl3 1:1:1 P2: Blood:MeOH:CHCl3 1:2.5:2.5 P4: Blood:MeOH:CHCl3 1:8

Sample Preparation:

Sampleprep ID:SP002054
Sampleprep Summary:-

Combined analysis:

Analysis ID AN003206 AN003207 AN003208 AN003209
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase HILIC HILIC
Chromatography system Phenomenex Phenomenex Waters Waters
Column Phenomenex Kinetex XB-C18 (1.7µm 100 A 150x 2.1mm) Phenomenex Kinetex XB-C18 (1.7µm 100 A 150x 2.1mm) Waters Cortecs (unbonded silica 1.6µm 100 A 150x2.1mm) Waters Cortecs (unbonded silica 1.6µm 100 A 150x2.1mm)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units SI SI SI SI

Chromatography:

Chromatography ID:CH002369
Instrument Name:Phenomenex
Column Name:Phenomenex Kinetex XB-C18 (1.7µm 100 A 150x 2.1mm)
Chromatography Type:Reversed phase
  
Chromatography ID:CH002370
Instrument Name:Waters
Column Name:Waters Cortecs (unbonded silica 1.6µm 100 A 150x2.1mm)
Chromatography Type:HILIC

MS:

MS ID:MS002982
Analysis ID:AN003206
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS002983
Analysis ID:AN003207
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
  
MS ID:MS002984
Analysis ID:AN003208
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS002985
Analysis ID:AN003209
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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