Summary of Study ST002042

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001289. The data can be accessed directly via it's Project DOI: 10.21228/M83D80 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002042
Study TitleLong-term metabolic profiling in transient middle cerebral artery occlusion (tMCAO) mouse model
Study Summarywe investigated the long-term metabolic changes caused by stroke in a transient middle cerebral artery occlusion (tMCAO).
Institute
KIST
Last NameNaeun
First NameYoon
Address5, Hwarang ro 14 gil, songbuk-gu, Seoul
Emailbetteryun@kist.re.kr
Phone82-10-9091-5149
Submit Date2021-12-23
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-05-31
Release Version1
Yoon Naeun Yoon Naeun
https://dx.doi.org/10.21228/M83D80
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001289
Project DOI:doi: 10.21228/M83D80
Project Title:Investigation of long-term metabolic alteration after stroke in tMCAO (transient middle cerebral artery occlusion) mouse model using metabolomics approach
Project Summary:Investigation of changes in brain damage and long-term metabolic changes caused by stroke in a transient middle cerebral artery occlusion (tMCAO)
Institute:Korea institute of Science and Technology
Last Name:Yoon
First Name:Naeun
Address:5, hwa-rang ro 14 gil, Seong-buk, Seoul, 02751, Korea
Email:Southbetteryun@kist.re.kr
Phone:029585145

Subject:

Subject ID:SU002163
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id time point
SA196440N_81-day
SA196441N_71-day
SA196442sham_11-day
SA196443sham_21-day
SA196444sham_41-day
SA196445sham_31-day
SA1964461dM_11-day
SA196447N_11-day
SA196448N_21-day
SA196449N_41-day
SA196450N_51-day
SA196451N_61-day
SA196452N_31-day
SA196453sham_51-day
SA1964541dM_61-day
SA1964551dM_71-day
SA1964561dM_91-day
SA1964571dM_51-day
SA1964581dM_41-day
SA1964591dM_21-day
SA1964601dM_31-day
SA1964611dM_101-day
SA1964621dM_81-day
SA1964631dM_131-day
SA1964641dM_111-day
SA1964651dM_121-day
SA1964661mM_11-month
SA1964671mM_21-month
SA1964681mM_41-month
SA1964691mM_51-month
SA196470sham_111-month
SA1964711mM_31-month
SA196472sham_141-month
SA196473sham_131-month
SA196474sham_121-month
SA196475sham_101-month
SA1964761wM_61-week
SA1964771wM_41-week
SA1964781wM_31-week
SA1964791wM_21-week
SA1964801wM_11-week
SA1964811wM_51-week
SA196482sham_61-week
SA196483sham_81-week
SA196484sham_91-week
SA1964851wM_71-week
SA196486sham_71-week
SA1964876mM_46-month
SA1964886mM_36-month
SA1964896mM_26-month
SA1964906mM_16-month
SA1964916mM_56-month
SA1964926mM_66-month
SA1964936mS_36-month
SA1964946mS_26-month
SA1964956mS_16-month
SA1964966mS_46-month
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Collection:

Collection ID:CO002156
Collection Summary:Plasma was obtained from the blood samples upon centrifugation and stored at −80 °C until analysis.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR002175
Treatment Summary:The mouse model was induced via MCAO using a 6-0 nylon monolayer suture. Animals were anesthetized with 2% isoflurane in 30% oxygen and 70% nitrous oxide using a face mask. The mice were anesthetized, and blood was collected by retro-orbital bleeding using sterile capillary tubes.

Sample Preparation:

Sampleprep ID:SP002169
Sampleprep Summary:plasma samples were processed through a simple protein precipitation technique using 150 µL of ice-cold methanol using plasma methanol ratio of 1:3. After the addition of methanol, the mixture was then vortexed and centrifuged at 20,800×g for 10 min at 4 °C.

Combined analysis:

Analysis ID AN003393
Analysis type MS
Chromatography type Reversed phase
Chromatography system Orbitrap Velos Pro mass spectrometer system
Column Waters ACQUITY UPLC BEH C18
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Velos LTQ Orbitrap
Ion Mode NEGATIVE
Units peak area

Chromatography:

Chromatography ID:CH002508
Instrument Name:Orbitrap Velos Pro mass spectrometer system
Column Name:Waters ACQUITY UPLC BEH C18
Chromatography Type:Reversed phase

MS:

MS ID:MS003160
Analysis ID:AN003393
Instrument Name:Thermo Velos LTQ Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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