Summary of Study ST002086

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001325. The data can be accessed directly via it's Project DOI: 10.21228/M8FM6D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002086
Study TitleSerum NMR profiling reveals differential alterations in the lipoproteome induced by Pfizer-BioNTech vaccine in COVID-19–recovered subjects and naïve subjects
Study TypeNMR-based metabolomics
Study Summary1H NMR spectra of sera have been used to define the changes induced by vaccination with Pfizer-BioNTech vaccine (2 shots, 21 days apart) in 10 COVID-19-recovered subjects and 10 COVID-19-naïve subjects at different time points, starting from before vaccination, then weekly until 7 days after second injection, and finally 1 month after the second dose. The data show that vaccination does not induce any significant variation in the metabolome, whereas it causes changes at the level of lipoproteins. The effects are different in the COVID-19-recovered subjects with respect to the naïve subjects, suggesting that a previous infection reduces the vaccine modulation of the lipoproteome composition.
Institute
University of Florence
Last NameGhini
First NameVeronica
Addressvia Luigi Sacconi
Emailghini@cerm.unifi.it
Phone03922800462
Submit Date2022-02-14
Num Groups2
Total Subjects20
Num Males7
Num Females13
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2022-02-28
Release Version1
Veronica Ghini Veronica Ghini
https://dx.doi.org/10.21228/M8FM6D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001325
Project DOI:doi: 10.21228/M8FM6D
Project Title:Serum NMR profiling reveals differential alterations in the lipoproteome induced by Pfizer-BioNTech vaccine in COVID-19–recovered subjects and naïve subjects
Project Type:NMR-based metabolomic analysis
Project Summary:1H NMR spectra of sera have been used to define the changes induced by vaccination with Pfizer-BioNTech vaccine (2 shots, 21 days apart) in 10 COVID-19-recovered subjects and 10 COVID-19-naïve subjects at different time points, starting from before vaccination, then weekly until 7 days after second injection, and finally 1 month after the second dose. The data show that vaccination does not induce any significant variation in the metabolome, whereas it causes changes at the level of lipoproteins. The effects are different in the COVID-19-recovered subjects with respect to the naïve subjects, suggesting that a previous infection reduces the vaccine modulation of the lipoproteome composition.
Institute:University of Florence
Department:Department of chemistry
Laboratory:Metabolomics
Last Name:Ghini
First Name:Veronica
Address:via Luigi Sacconi 6
Email:ghini@cerm.unifi.it
Phone:+390554574266

Subject:

Subject ID:SU002170
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:24-65
Gender:Male and female
Human Ethnicity:Caucasian

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Previus_COVID TIME
SA19832065-Serum_MF_T0No T0
SA1983211-Serum_AF_T0No T0
SA19832253-Serum_GT_T0No T0
SA19832371-Serum_MC_T0No T0
SA19832488-Serum_PS_T0No T0
SA198325111-Serum_VS_T0No T0
SA19832694-Serum_RA_T0No T0
SA19832730-Serum_CMG_T0No T0
SA19832859-Serum_KT_T0No T0
SA1983297-Serum_CI_T0No T0
SA19833067-Serum_MF_T14No T14
SA19833132-Serum_CMG_T14No T14
SA19833261-Serum_KT_T14No T14
SA1983339-Serum_CI_T14No T14
SA19833455-Serum_GT_T14No T14
SA19833590-Serum_PS_T14No T14
SA19833673-Serum_MC_T14No T14
SA1983373-Serum_AF_T14No T14
SA19833896-Serum_RA_T14No T14
SA198339113-Serum_VS_T14No T14
SA19834058-Serum_GT_T1MNo T1M
SA198341116-Serum_VS_T1MNo T1M
SA19834235-Serum_CMG_T1MNo T1M
SA19834399-Serum_RA_T1MNo T1M
SA19834493-Serum_PS_T1MNo T1M
SA1983456-Serum_AF_T1MNo T1M
SA19834670-Serum_MF_T1MNo T1M
SA19834776-Serum_MC_T1MNo T1M
SA19834864-Serum_KT_T1MNo T1M
SA19834912-Serum_CI_T1MNo T1M
SA19835068-Serum_MF_T21No T21
SA19835162-Serum_KT_T21No T21
SA19835291-Serum_PS_T21No T21
SA19835397-Serum_RA_T21No T21
SA198354114-Serum_VS_T21No T21
SA19835556-Serum_GT_T21No T21
SA1983564-Serum_AF_T21No T21
SA19835774-Serum_MC_T21No T21
SA19835833-Serum_CMG_T21No T21
SA19835910-Serum_CI_T21_200ulNo T21
SA19836075-Serum_MC_T28No T28
SA19836163-Serum_KT_T28_280ulNo T28
SA19836234-Serum_CMG_T28No T28
SA19836369-Serum_MF_T28No T28
SA19836411-Serum_CI_T28No T28
SA1983655-Serum_AF_T28No T28
SA198366115-Serum_VS_T28No T28
SA19836792-Serum_PS_T28No T28
SA19836857-Serum_GT_T28No T28
SA19836998-Serum_RA_T28No T28
SA19837060-Serum_KT_T07No T7
SA19837172-Serum_MC_T07No T7
SA19837295-Serum_RA_T07_250uLNo T7
SA19837389-Serum_PS_T7No T7
SA198374112-Serum_VS_T07No T7
SA1983758-Serum_CI_T07No T7
SA19837666-Serum_MF_T7No T7
SA19837731-Serum_CMG_T7No T7
SA19837854-Serum_GT_T7No T7
SA1983792-Serum_AF_T7No T7
SA19838083-Serum_PF_T0Yes T0
SA198381100-Serum_SA_T0Yes T0
SA19838219-Serum_CCa_T07Yes T0
SA19838313-Serum_CC_T07_250uLYes T0
SA19838448-Serum_DD_T0Yes T0
SA19838577-Serum_NF_T0Yes T0
SA198386106-Serum_SB_T0Yes T0
SA19838742-Serum_CA_T0Yes T0
SA19838825-Serum_CS_T0Yes T0
SA19838936-Serum_CG_T0Yes T0
SA19839044-Serum_CA_T14Yes T14
SA19839179-Serum_NF_T14Yes T14
SA19839215-Serum_CC_T21Yes T14
SA198393107-Serum_SB_T14Yes T14
SA19839427-Serum_CS_T14Yes T14
SA19839585-Serum_PF_T14Yes T14
SA198396102-Serum_SA_T14Yes T14
SA19839721-Serum_CCa_T21Yes T14
SA19839838-Serum_CG_T14Yes T14
SA19839987-Serum_PF_T1MYes T1M
SA19840041-Serum_CG_T1MYes T1M
SA198401105-Serum_SA_T1MYes T1M
SA19840218-Serum_CC_T1MYes T1M
SA19840347-Serum_CA_T1MYes T1M
SA19840452-Serum_DD_T1MYes T1M
SA19840524-Serum_CCa_T1MYes T1M
SA19840682-Serum_NF_T1MYes T1M
SA198407110-Serum_SB_T1MYes T1M
SA19840845-Serum_CA_T21Yes T21
SA19840950-Serum_DD_T21Yes T21
SA198410103-Serum_SA_T21Yes T21
SA19841128-Serum_CS_T21Yes T21
SA198412108-Serum_SB_T21Yes T21
SA19841339-Serum_CG_T21Yes T21
SA19841486-Serum_PF_T21Yes T21
SA19841522-Serum_CCa_T28Yes T21
SA19841680-Serum_NF_T21Yes T21
SA19841716-Serum_CC_T28Yes T21
SA19841817-Serum_CC_T0_250uLYes T28
SA19841981-Serum_NF_T28Yes T28
Showing page 1 of 2     Results:    1  2  Next     Showing results 1 to 100 of 116

Collection:

Collection ID:CO002163
Collection Summary:For all subjects blood serum samples were collected at six different time points: before the first dose (T0); 7 and 14 days after the first dose (T7 and T14, respectively); 21 days after the first dose, just before the second dose (T21); 28 days after the first dose and 7 days after the second dose (T28); 1 month after the second dose (T1M). Blood samples were collected (4 hours after breakfast) in a BD vacutainer clot-activator tube for serum collection and processed within one hour from sample collection. After processing, all the serum samples were immediately stored at -30°C until NMR analysis.
Sample Type:Blood (serum)
Storage Conditions:Described in summary
Collection Vials:BD vacutainer clot-activator tube for serum

Treatment:

Treatment ID:TR002182
Treatment Summary:We monitor the effect of mRNA Pfizer-BioNTech vaccine in a cohort of 20 healthcare workers. All of them received two doses, 21 days apart.
Treatment:Pfizer-BioNTech mRNA vaccine

Sample Preparation:

Sampleprep ID:SP002176
Sampleprep Summary:NMR samples were prepared according to standard procedures. Frozen serum samples were thawed at room temperature. A total of 350 μL of sodium phosphate buffer (70 mM Na2HPO4; 20% (v/v) 2H2O; 6.1 mM NaN3, 4.6 mM sodium trimethylsilyl [2,2,3,3−2H4] propionate (TMSP), pH 7.4) was added to 350 μL of each serum sample; the mixture was homogenized by vortexing for 30 s. A total of 600 μL of each mixture was transferred into a 5.00 mm NMR tube (Bruker BioSpin) for the analysis.
Processing Storage Conditions:-80℃

Analysis:

Analysis ID:AN003404
Analysis Type:NMR
Num Factors:12
Num Metabolites:114
Units:mM

NMR:

NMR ID:NM000230
Analysis ID:AN003404
Instrument Name:Bruker
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600MHz
NMR Probe:5 mm PATXI 1H−13C−15N and 2H-decoupling probe
NMR Tube Size:5.00 mm
Pulse Sequence:NOESY
Water Suppression:1Dpresat
Temperature:36,85
Number Of Scans:32
Dummy Scans:4
Acquisition Time:2.7 s
Relaxation Delay:4 s
Spectral Width:18,028 Hz
Num Data Points Acquired:98,304
Line Broadening:0.3
Binned Increment:0.02 ppm
Binned Data Excluded Range:4.5-5.5 ppm
NMR Results File:NOESY.txt UNITS:ppm
  logo