Summary of Study ST002211
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001412. The data can be accessed directly via it's Project DOI: 10.21228/M86M67 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST002211 |
Study Title | Effects of Medwakh Smoking on Oxidative Stress and Inflammation Among Youth in UAE using Liquid chromatography-mass spectrometry |
Study Summary | Oxidative stress and inflammation are closely related, and inter-dependent pathophysiological processes. Oxidative stress plays a key role in the pathophysiology of several chronic inflammatory conditions that causes cellular alterations such as lipid damage, increase of cell permeability, apoptosis/cell death, alteration in growth factors, and signaling pathways.An imbalance between the production of reactive oxygen species (ROS) and their neutralization by the antioxidant system results in the onset of oxidative stress which subsequently leads to the release of chronic inflammatory mediators such as pro-inflammatory cytokines, which causes damages to DNA, proteins, and lipids.LC-MSMS is widely used for non-targeted and non-invasive metabolomics of biological samples including saliva in a variety of conditions such as inflammatory conditions, cancers, diabetes, etc. However, to the best of our knowledge, there are currently no metabolomics-based studies identifying salivary biomarkers associated with inflammation and oxidative stress utilizing HPLC-ESI-QTOF-MS. Therefore, in this study, we aim to discover the biochemical and physiological changes induced by medwak smoking and explore the metabolomics profile of medwak smokers with a focus on the markers of oxidative stress and inflammation in comparison to nonsmokers. |
Institute | Sharjah Institute for Medical Research |
Department | Wound Healing and Oral Diagnostics |
Laboratory | Sharjah Institute for Medical Research |
Last Name | K.G |
First Name | Aghila Rani |
Address | Sharjah, UAE |
anair@sharjah.ac.ae | |
Phone | 0562206252 |
Submit Date | 2022-06-20 |
Num Groups | 2 |
Total Subjects | 60 |
Num Males | 60 |
Study Comments | NA |
Publications | NA |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2024-01-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR001412 |
Project DOI: | doi: 10.21228/M86M67 |
Project Title: | Effects of Medwakh Smoking on Oxidative Stress and Inflammation Among Youth in UAE using Liquid chromatography-mass spectrometry |
Project Type: | Untargeted metabolomics |
Project Summary: | The use of alternative tobacco products is rampant among the youth in the Middle East and currently spreading worldwide. It is speculated that public policies that have proven effective in limiting cigarette usage and facilitating cessation of its use resulted in the widespread use of alternative tobacco products (ATPs) as major forms of tobacco delivery systems. Previous studies have reported the acute effects of smoking medwak on altering the systolic blood pressure, cardiovascular and respiratory systems. Smoking medwakh results in the release of a mixture of toxic chemicals including nicotine, carbon monoxide, and oxidizing gases 8-10. Inhalation of these toxic gases results in triggering of oxidative stress pathways that leads to endothelial dysfunction, inflammation, and platelet activation 11,12. Therefore, in this study, we aim to discover the biochemical and physiological changes induced by medwak smoking and explore the metabolomics profile of medwak smokers with a focus on the markers of oxidative stress and inflammation in comparison to nonsmokers. |
Institute: | University of Sharjah |
Department: | Wound Healing and Oral Diagnostics |
Laboratory: | Sharjah Institute for Medical Research |
Last Name: | K.G |
First Name: | Aghila Rani |
Address: | Sharjah, UAE |
Email: | anair@sharjah.ac.ae |
Phone: | 0562206252 |
Funding Source: | Al Jalila |
Project Comments: | NA |
Publications: | NA |
Contributors: | NA |
Subject:
Subject ID: | SU002297 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 17-25 |
Gender: | Male |
Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment |
---|---|---|
SA211536 | Saliva C23-01-5592 | Non-smoker |
SA211537 | Saliva C22-02-5591 | Non-smoker |
SA211538 | Saliva C23-02-5593 | Non-smoker |
SA211539 | Saliva C24-02-5595 | Non-smoker |
SA211540 | Saliva C26-02-5597 | Non-smoker |
SA211541 | Saliva C22-01-5590 | Non-smoker |
SA211542 | Saliva C24-01-5594 | Non-smoker |
SA211543 | Saliva C21-02-5589 | Non-smoker |
SA211544 | Saliva C19-02-5585 | Non-smoker |
SA211545 | Saliva C19-01-5584 | Non-smoker |
SA211546 | Saliva C20-01-5586 | Non-smoker |
SA211547 | Saliva C20-02-5587 | Non-smoker |
SA211548 | Saliva C21-01-5588 | Non-smoker |
SA211549 | Saliva C28-01-5598 | Non-smoker |
SA211550 | Saliva C29-01-5600 | Non-smoker |
SA211551 | Saliva C33-01-5617 | Non-smoker |
SA211552 | Saliva C32-02-5616 | Non-smoker |
SA211553 | Saliva C33-02-5618 | Non-smoker |
SA211554 | Saliva C34-01-5619 | Non-smoker |
SA211555 | Saliva C01-01-5551 | Non-smoker |
SA211556 | Saliva C34-02-5620 | Non-smoker |
SA211557 | Saliva C32-01-5615 | Non-smoker |
SA211558 | Saliva C31-02-5614 | Non-smoker |
SA211559 | Saliva C29-02-5601 | Non-smoker |
SA211560 | Saliva C18-02-5583 | Non-smoker |
SA211561 | Saliva C30-01-5602 | Non-smoker |
SA211562 | Saliva C30-02-5603 | Non-smoker |
SA211563 | Saliva C31-01-5613 | Non-smoker |
SA211564 | Saliva C28-02-5599 | Non-smoker |
SA211565 | Saliva C26-01-5596 | Non-smoker |
SA211566 | Saliva C07-01-5561 | Non-smoker |
SA211567 | Saliva C05-02-5560 | Non-smoker |
SA211568 | Saliva C07-02-5562 | Non-smoker |
SA211569 | Saliva C08-01-5563 | Non-smoker |
SA211570 | Saliva C09-01-5565 | Non-smoker |
SA211571 | Saliva C08-02-5564 | Non-smoker |
SA211572 | Saliva C05-01-5559 | Non-smoker |
SA211573 | Saliva C04-02-5558 | Non-smoker |
SA211574 | Saliva C18-01-5582 | Non-smoker |
SA211575 | Saliva C01-02-5552 | Non-smoker |
SA211576 | Saliva C02-02-5554 | Non-smoker |
SA211577 | Saliva C03-01-5555 | Non-smoker |
SA211578 | Saliva C04-01-5557 | Non-smoker |
SA211579 | Saliva C03-02-5556 | Non-smoker |
SA211580 | Saliva C09-02-5566 | Non-smoker |
SA211581 | Saliva C02-01-5553 | Non-smoker |
SA211582 | Saliva C15-02-5576 | Non-smoker |
SA211583 | Saliva C15-01-5575 | Non-smoker |
SA211584 | Saliva C16-01-5577 | Non-smoker |
SA211585 | Saliva C16-02-5578 | Non-smoker |
SA211586 | Saliva C11-01-5567 | Non-smoker |
SA211587 | Saliva C17-02-5580 | Non-smoker |
SA211588 | Saliva C14-02-5574 | Non-smoker |
SA211589 | Saliva C17-01-5579 | Non-smoker |
SA211590 | Saliva C12-01-5569 | Non-smoker |
SA211591 | Saliva C11-02-5568 | Non-smoker |
SA211592 | Saliva C14-01-5573 | Non-smoker |
SA211593 | Saliva C12-02-5570 | Non-smoker |
SA211594 | Saliva C13-01-5571 | Non-smoker |
SA211595 | Saliva C13-02-5572 | Non-smoker |
SA211596 | Saliva T21-01-5664 | Smoker |
SA211597 | Saliva T20-02-5663 | Smoker |
SA211598 | Saliva T23-01-5668 | Smoker |
SA211599 | Saliva T22-02-5667 | Smoker |
SA211600 | Saliva T22-01-5666 | Smoker |
SA211601 | Saliva T21-02-5665 | Smoker |
SA211602 | Saliva T18-02-5659 | Smoker |
SA211603 | Saliva T17-01-5656 | Smoker |
SA211604 | Saliva T23-02-5669 | Smoker |
SA211605 | Saliva T17-02-5657 | Smoker |
SA211606 | Saliva T18-01-5658 | Smoker |
SA211607 | Saliva T19-02-5661 | Smoker |
SA211608 | Saliva T19-01-5660 | Smoker |
SA211609 | Saliva T20-01-5662 | Smoker |
SA211610 | Saliva T27-02-5677 | Smoker |
SA211611 | Saliva T29-01-5680 | Smoker |
SA211612 | Saliva T28-02-5679 | Smoker |
SA211613 | Saliva T29-02-5681 | Smoker |
SA211614 | Saliva T30-01-5682 | Smoker |
SA211615 | Saliva T16-02-5655 | Smoker |
SA211616 | Saliva T30-02-5683 | Smoker |
SA211617 | Saliva T28-01-5678 | Smoker |
SA211618 | Saliva T27-01-5676 | Smoker |
SA211619 | Saliva T25-01-5672 | Smoker |
SA211620 | Saliva T24-02-5671 | Smoker |
SA211621 | Saliva T25-02-5673 | Smoker |
SA211622 | Saliva T26-01-5674 | Smoker |
SA211623 | Saliva T26-02-5675 | Smoker |
SA211624 | Saliva T24-01-5670 | Smoker |
SA211625 | Saliva T01-01-5623 | Smoker |
SA211626 | Saliva T06-01-5633 | Smoker |
SA211627 | Saliva T05-02-5632 | Smoker |
SA211628 | Saliva T06-02-5634 | Smoker |
SA211629 | Saliva T07-01-5635 | Smoker |
SA211630 | Saliva T08-01-5637 | Smoker |
SA211631 | Saliva T07-02-5636 | Smoker |
SA211632 | Saliva T05-01-5631 | Smoker |
SA211633 | Saliva T04-02-5630 | Smoker |
SA211634 | Saliva T02-01-5625 | Smoker |
SA211635 | Saliva T01-02-5624 | Smoker |
Collection:
Collection ID: | CO002290 |
Collection Summary: | Saliva samples were collected from 30 non smokers and 30 smokers. Participants were instructed to observe a two-hour fast without eating, smoking or drinking followed by a thorough rinsing of the mouth to presvent any sample contamination. Subjects were instructed to allow saliva to pool in the bottom of the mouth and drain it into sterile collection tubes. The samples were transferred to the lab in ice containers and centrifuged at 2500 rpm for 5 minutes at 4°C to remove cell debris, and the supernatants stored at 80°C until analysis. |
Sample Type: | Saliva |
Treatment:
Treatment ID: | TR002309 |
Treatment Summary: | Saliva samples were collected from non smokers and smokers. Participants were instructed to observe a two-hour fast without eating, smoking or drinking followed by a thorough rinsing of the mouth to presvent any sample contamination. Subjects were instructed to allow saliva to pool in the bottom of the mouth and drain it into sterile collection tubes. The samples were transferred to the lab in ice containers and centrifuged at 2500 rpm for 5 minutes at 4°C to remove cell debris, and the supernatants stored at 80°C until analysis. |
Sample Preparation:
Sampleprep ID: | SP002303 |
Sampleprep Summary: | Protein was precipitated out via chloroform methanol precipitation and the metabolite containing supernatants taken forward for analysis. |
Combined analysis:
Analysis ID | AN003615 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Bruker Elute |
Column | Hamilton Intensity Solo 2 C18 |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | timsTOF |
Ion Mode | POSITIVE |
Units | AU |
Chromatography:
Chromatography ID: | CH002670 |
Chromatography Summary: | uHPLC C18 Reversed phase |
Instrument Name: | Bruker Elute |
Column Name: | Hamilton Intensity Solo 2 C18 |
Column Temperature: | 35 |
Flow Gradient: | 1%B to 99%B in 15 min |
Flow Rate: | 250 uL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003366 |
Analysis ID: | AN003615 |
Instrument Name: | timsTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | MS2 data were acquired in data-dependent acquisition using a fixed cycle time of 3s and using a scan range of 20 - 1,300 Metaboscape 4.0 was used for feature detection, retention time alignment, deisotoping and metabolite identification. |
Ion Mode: | POSITIVE |