Summary of Study ST002211

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001412. The data can be accessed directly via it's Project DOI: 10.21228/M86M67 This work is supported by NIH grant, U2C- DK119886.

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Study IDST002211
Study TitleEffects of Medwakh Smoking on Oxidative Stress and Inflammation Among Youth in UAE using Liquid chromatography-mass spectrometry
Study SummaryOxidative stress and inflammation are closely related, and inter-dependent pathophysiological processes. Oxidative stress plays a key role in the pathophysiology of several chronic inflammatory conditions that causes cellular alterations such as lipid damage, increase of cell permeability, apoptosis/cell death, alteration in growth factors, and signaling pathways.An imbalance between the production of reactive oxygen species (ROS) and their neutralization by the antioxidant system results in the onset of oxidative stress which subsequently leads to the release of chronic inflammatory mediators such as pro-inflammatory cytokines, which causes damages to DNA, proteins, and lipids.LC-MSMS is widely used for non-targeted and non-invasive metabolomics of biological samples including saliva in a variety of conditions such as inflammatory conditions, cancers, diabetes, etc. However, to the best of our knowledge, there are currently no metabolomics-based studies identifying salivary biomarkers associated with inflammation and oxidative stress utilizing HPLC-ESI-QTOF-MS. Therefore, in this study, we aim to discover the biochemical and physiological changes induced by medwak smoking and explore the metabolomics profile of medwak smokers with a focus on the markers of oxidative stress and inflammation in comparison to nonsmokers.
Institute
Sharjah Institute for Medical Research
DepartmentWound Healing and Oral Diagnostics
LaboratorySharjah Institute for Medical Research
Last NameK.G
First NameAghila Rani
AddressSharjah, UAE
Emailanair@sharjah.ac.ae
Phone0562206252
Submit Date2022-06-20
Num Groups2
Total Subjects60
Num Males60
Study CommentsNA
PublicationsNA
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2024-01-01
Release Version1
Aghila Rani K.G Aghila Rani K.G
https://dx.doi.org/10.21228/M86M67
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001412
Project DOI:doi: 10.21228/M86M67
Project Title:Effects of Medwakh Smoking on Oxidative Stress and Inflammation Among Youth in UAE using Liquid chromatography-mass spectrometry
Project Type:Untargeted metabolomics
Project Summary:The use of alternative tobacco products is rampant among the youth in the Middle East and currently spreading worldwide. It is speculated that public policies that have proven effective in limiting cigarette usage and facilitating cessation of its use resulted in the widespread use of alternative tobacco products (ATPs) as major forms of tobacco delivery systems. Previous studies have reported the acute effects of smoking medwak on altering the systolic blood pressure, cardiovascular and respiratory systems. Smoking medwakh results in the release of a mixture of toxic chemicals including nicotine, carbon monoxide, and oxidizing gases 8-10. Inhalation of these toxic gases results in triggering of oxidative stress pathways that leads to endothelial dysfunction, inflammation, and platelet activation 11,12. Therefore, in this study, we aim to discover the biochemical and physiological changes induced by medwak smoking and explore the metabolomics profile of medwak smokers with a focus on the markers of oxidative stress and inflammation in comparison to nonsmokers.
Institute:University of Sharjah
Department:Wound Healing and Oral Diagnostics
Laboratory:Sharjah Institute for Medical Research
Last Name:K.G
First Name:Aghila Rani
Address:Sharjah, UAE
Email:anair@sharjah.ac.ae
Phone:0562206252
Funding Source:Al Jalila
Project Comments:NA
Publications:NA
Contributors:NA

Subject:

Subject ID:SU002297
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:17-25
Gender:Male
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA211536Saliva C23-01-5592Non-smoker
SA211537Saliva C22-02-5591Non-smoker
SA211538Saliva C23-02-5593Non-smoker
SA211539Saliva C24-02-5595Non-smoker
SA211540Saliva C26-02-5597Non-smoker
SA211541Saliva C22-01-5590Non-smoker
SA211542Saliva C24-01-5594Non-smoker
SA211543Saliva C21-02-5589Non-smoker
SA211544Saliva C19-02-5585Non-smoker
SA211545Saliva C19-01-5584Non-smoker
SA211546Saliva C20-01-5586Non-smoker
SA211547Saliva C20-02-5587Non-smoker
SA211548Saliva C21-01-5588Non-smoker
SA211549Saliva C28-01-5598Non-smoker
SA211550Saliva C29-01-5600Non-smoker
SA211551Saliva C33-01-5617Non-smoker
SA211552Saliva C32-02-5616Non-smoker
SA211553Saliva C33-02-5618Non-smoker
SA211554Saliva C34-01-5619Non-smoker
SA211555Saliva C01-01-5551Non-smoker
SA211556Saliva C34-02-5620Non-smoker
SA211557Saliva C32-01-5615Non-smoker
SA211558Saliva C31-02-5614Non-smoker
SA211559Saliva C29-02-5601Non-smoker
SA211560Saliva C18-02-5583Non-smoker
SA211561Saliva C30-01-5602Non-smoker
SA211562Saliva C30-02-5603Non-smoker
SA211563Saliva C31-01-5613Non-smoker
SA211564Saliva C28-02-5599Non-smoker
SA211565Saliva C26-01-5596Non-smoker
SA211566Saliva C07-01-5561Non-smoker
SA211567Saliva C05-02-5560Non-smoker
SA211568Saliva C07-02-5562Non-smoker
SA211569Saliva C08-01-5563Non-smoker
SA211570Saliva C09-01-5565Non-smoker
SA211571Saliva C08-02-5564Non-smoker
SA211572Saliva C05-01-5559Non-smoker
SA211573Saliva C04-02-5558Non-smoker
SA211574Saliva C18-01-5582Non-smoker
SA211575Saliva C01-02-5552Non-smoker
SA211576Saliva C02-02-5554Non-smoker
SA211577Saliva C03-01-5555Non-smoker
SA211578Saliva C04-01-5557Non-smoker
SA211579Saliva C03-02-5556Non-smoker
SA211580Saliva C09-02-5566Non-smoker
SA211581Saliva C02-01-5553Non-smoker
SA211582Saliva C15-02-5576Non-smoker
SA211583Saliva C15-01-5575Non-smoker
SA211584Saliva C16-01-5577Non-smoker
SA211585Saliva C16-02-5578Non-smoker
SA211586Saliva C11-01-5567Non-smoker
SA211587Saliva C17-02-5580Non-smoker
SA211588Saliva C14-02-5574Non-smoker
SA211589Saliva C17-01-5579Non-smoker
SA211590Saliva C12-01-5569Non-smoker
SA211591Saliva C11-02-5568Non-smoker
SA211592Saliva C14-01-5573Non-smoker
SA211593Saliva C12-02-5570Non-smoker
SA211594Saliva C13-01-5571Non-smoker
SA211595Saliva C13-02-5572Non-smoker
SA211596Saliva T21-01-5664Smoker
SA211597Saliva T20-02-5663Smoker
SA211598Saliva T23-01-5668Smoker
SA211599Saliva T22-02-5667Smoker
SA211600Saliva T22-01-5666Smoker
SA211601Saliva T21-02-5665Smoker
SA211602Saliva T18-02-5659Smoker
SA211603Saliva T17-01-5656Smoker
SA211604Saliva T23-02-5669Smoker
SA211605Saliva T17-02-5657Smoker
SA211606Saliva T18-01-5658Smoker
SA211607Saliva T19-02-5661Smoker
SA211608Saliva T19-01-5660Smoker
SA211609Saliva T20-01-5662Smoker
SA211610Saliva T27-02-5677Smoker
SA211611Saliva T29-01-5680Smoker
SA211612Saliva T28-02-5679Smoker
SA211613Saliva T29-02-5681Smoker
SA211614Saliva T30-01-5682Smoker
SA211615Saliva T16-02-5655Smoker
SA211616Saliva T30-02-5683Smoker
SA211617Saliva T28-01-5678Smoker
SA211618Saliva T27-01-5676Smoker
SA211619Saliva T25-01-5672Smoker
SA211620Saliva T24-02-5671Smoker
SA211621Saliva T25-02-5673Smoker
SA211622Saliva T26-01-5674Smoker
SA211623Saliva T26-02-5675Smoker
SA211624Saliva T24-01-5670Smoker
SA211625Saliva T01-01-5623Smoker
SA211626Saliva T06-01-5633Smoker
SA211627Saliva T05-02-5632Smoker
SA211628Saliva T06-02-5634Smoker
SA211629Saliva T07-01-5635Smoker
SA211630Saliva T08-01-5637Smoker
SA211631Saliva T07-02-5636Smoker
SA211632Saliva T05-01-5631Smoker
SA211633Saliva T04-02-5630Smoker
SA211634Saliva T02-01-5625Smoker
SA211635Saliva T01-02-5624Smoker
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Collection:

Collection ID:CO002290
Collection Summary:Saliva samples were collected from 30 non smokers and 30 smokers. Participants were instructed to observe a two-hour fast without eating, smoking or drinking followed by a thorough rinsing of the mouth to presvent any sample contamination. Subjects were instructed to allow saliva to pool in the bottom of the mouth and drain it into sterile collection tubes. The samples were transferred to the lab in ice containers and centrifuged at 2500 rpm for 5 minutes at 4°C to remove cell debris, and the supernatants stored at 80°C until analysis.
Sample Type:Saliva

Treatment:

Treatment ID:TR002309
Treatment Summary:Saliva samples were collected from non smokers and smokers. Participants were instructed to observe a two-hour fast without eating, smoking or drinking followed by a thorough rinsing of the mouth to presvent any sample contamination. Subjects were instructed to allow saliva to pool in the bottom of the mouth and drain it into sterile collection tubes. The samples were transferred to the lab in ice containers and centrifuged at 2500 rpm for 5 minutes at 4°C to remove cell debris, and the supernatants stored at 80°C until analysis.

Sample Preparation:

Sampleprep ID:SP002303
Sampleprep Summary:Protein was precipitated out via chloroform methanol precipitation and the metabolite containing supernatants taken forward for analysis.

Combined analysis:

Analysis ID AN003615
Analysis type MS
Chromatography type Reversed phase
Chromatography system Bruker Elute
Column Hamilton Intensity Solo 2 C18
MS Type ESI
MS instrument type QTOF
MS instrument name timsTOF
Ion Mode POSITIVE
Units AU

Chromatography:

Chromatography ID:CH002670
Chromatography Summary:uHPLC C18 Reversed phase
Instrument Name:Bruker Elute
Column Name:Hamilton Intensity Solo 2 C18
Column Temperature:35
Flow Gradient:1%B to 99%B in 15 min
Flow Rate:250 uL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS003366
Analysis ID:AN003615
Instrument Name:timsTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:MS2 data were acquired in data-dependent acquisition using a fixed cycle time of 3s and using a scan range of 20 - 1,300 Metaboscape 4.0 was used for feature detection, retention time alignment, deisotoping and metabolite identification.
Ion Mode:POSITIVE
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