Summary of Study ST002278

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001459. The data can be accessed directly via it's Project DOI: 10.21228/M84H7Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002278
Study TitleModulation of blood metabolites by dietary β–glucan in rainbow trout (Oncorhynchus mykiss) - Serum lipid concentrations
Study SummaryMeasurement of changes in serum lipid levels using targeted mass spectrometry
Institute
Helmholtz Centre for Environmental Research
Last NameHaange
First NameSven
AddressPermoserstrasse 1, Leipzig, Saxony, 04318, Germany
Emailsven.haange@ufz.de
Phone0049 341 2351099
Submit Date2022-08-19
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2022-09-22
Release Version1
Sven Haange Sven Haange
https://dx.doi.org/10.21228/M84H7Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001459
Project DOI:doi: 10.21228/M84H7Z
Project Title:Modulation of blood metabolites by dietary β–glucan in rainbow trout (Oncorhynchus mykiss)
Project Summary:Prebiotics are known to have a positive impact on fish health and growth rate, and β-glucans are among the most used prebiotics on the market. In this study, rainbow trout (Oncorhynchus mykiss) were treated with a β-1,3;1,6-glucan dietary supplement (at a dose of 0 g, 1 g, 10 g, and 50 g β-glucan per kg of feed). After 6 weeks, the effect of the β-glucan was evaluated by determining the changes in the microbiota and the blood serum metabolites in the fish
Institute:Helmholtz Centre for Environmental Research - UFZ
Department:Molecular Systems Biology
Laboratory:Functional Metabolomics
Last Name:Haange
First Name:Sven
Address:Permoserstrasse 1, Leipzig, Saxony, 04318, Germany
Email:sven.haange@ufz.de
Phone:0049 341 2351099

Subject:

Subject ID:SU002364
Subject Type:Fish
Subject Species:Oncorhynchus mykiss

Factors:

Subject type: Fish; Subject species: Oncorhynchus mykiss (Factor headings shown in green)

mb_sample_id local_sample_id Sample time Treatment
SA21835610240257260h no ß-glucan
SA21835710240258090h no ß-glucan
SA21835810240257980h no ß-glucan
SA21835910240257450h no ß-glucan
SA21836010240257110h no ß-glucan
SA21836110240257500h no ß-glucan
SA21836210240257310h no ß-glucan
SA21836310240257790h no ß-glucan
SA21836410240257640h no ß-glucan
SA21836510240257830h no ß-glucan
SA21836610240288826_weeks 0,1% ß-glucan
SA21836710240196586_weeks 0,1% ß-glucan
SA21836810240288976_weeks 0,1% ß-glucan
SA21836910240288256_weeks 0,1% ß-glucan
SA21837010240288116_weeks 0,1% ß-glucan
SA21837110240288066_weeks 0,1% ß-glucan
SA21837210240289276_weeks 0,1% ß-glucan
SA21837310240287766_weeks 0,1% ß-glucan
SA21837410240288446_weeks 0,1% ß-glucan
SA21837510240289126_weeks 0,1% ß-glucan
SA21837610240289086_weeks 0,1% ß-glucan
SA21837710240288306_weeks 0,1% ß-glucan
SA21837810240288596_weeks 0,1% ß-glucan
SA21837910240289316_weeks 0,1% ß-glucan
SA21838010240287956_weeks 0,1% ß-glucan
SA21838110240288786_weeks 0,1% ß-glucan
SA21838210240196396_weeks 0,1% ß-glucan
SA21838310240288636_weeks 0,1% ß-glucan
SA21838410240287816_weeks 0,1% ß-glucan
SA21838510240196436_weeks 0,1% ß-glucan
SA21838610240197076_weeks 5% ß-glucan
SA21838710240197506_weeks 5% ß-glucan
SA21838810240196776_weeks 5% ß-glucan
SA21838910240197456_weeks 5% ß-glucan
SA21839010240197646_weeks 5% ß-glucan
SA21839110240196816_weeks 5% ß-glucan
SA21839210240197316_weeks 5% ß-glucan
SA21839310240196966_weeks 5% ß-glucan
SA21839410240197266_weeks 5% ß-glucan
SA21839510240197116_weeks 5% ß-glucan
SA21839610240258666_weeks no ß-glucan
SA21839710240258716_weeks no ß-glucan
SA21839810240258856_weeks no ß-glucan
SA21839910240258516_weeks no ß-glucan
SA21840010240258906_weeks no ß-glucan
SA21840110240258136_weeks no ß-glucan
SA21840210240258286_weeks no ß-glucan
SA21840310240287576_weeks no ß-glucan
SA21840410240258326_weeks no ß-glucan
SA21840510240258476_weeks no ß-glucan
Showing results 1 to 50 of 50

Collection:

Collection ID:CO002357
Collection Summary:For the metabolite and microbiota analyses, 10 fish from the control group were collected on day 0 and 10 fish from each group (control, 0.1, 1.0, 5.0% β-glucan) at week 6. Fish were euthanized by immersion in an overdose (200 mg∙L-1) of MS-222 (cat no. A5040, Sigma-Aldrich). The tail was cut, and blood was sampled from the vena caudalis using Na-heparinised 25 mL and 50 mL capillary pipettes (Hirschmann Laborgeräte, Germany). Blood samples were centrifuged at 3,000 g for 10 min at 4 °C, and serum was isolated and stored at -80 °C. The intestine was aseptically sampled and immediately immersed in RNAlater® (Sigma-Aldrich) and transferred to -20 °C.
Sample Type:Blood (serum)
Storage Conditions:-20℃

Treatment:

Treatment ID:TR002376
Treatment Summary:A commercial pelleted (1.5 mm) trout feed (INICIO 917, BioMar A/S) based on a mixture of fish and plant protein and containing 47% crude protein, 20% crude lipid, 18% carbohydrates (NFE), 1.2% fibre, 8.5% ash, and 1.1% phosphorus was used for preparation of the diets. A β-1,3;1,6-glucan (purity: 81.6%; mean particle size: 37.7 µm; supplementary table S2) purified from yeast (Saccharomyces cerevisiae) (Biorigin, Brazil) was supplemented to this diet at varying doses: 0 g, 1 g, 10 g, and 50 g β-glucan were added to 1 kg of INICIO 917, respectively, during continuous mixing and sealed to the pellets by spraying with 30 mL organic, cold-pressed rapeseed oil (Gyldenmark), resulting in a control diet without β-glucan and three experimental diets of 0.1%, 1.0%, and 5.0% Wt/Wt β-glucan.

Sample Preparation:

Sampleprep ID:SP002370
Sampleprep Summary:Sample preparation for MetIDQ p180 Kit measurement Solvents: Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS) Water MiliQ, Extracting agent - ACN / H2O (1:1) Equipment 4 steel balls size M Eppendorf Tubes 2mL Tissue slicer (Rettberg, Germany) Centrifuge (Sigma) Work steps Approximately 100 mg of sample and 4 steel balls of size M into each tube. Per mg of sample 5 µL of extracting agent was added. Shake the samples for 10 minutes at 30 Hz in the tissue slicer and centrifuge for 2 minutes at 14000 rpm. 10µL of supernatant was used for the targeted analytics. For blood sample analysis 10 µL plasma was taken. Kit reparation The analysis was performed using the validated MetIDQ p180 Kit and described in Siskos et al. [1]. Data processing was carried out with the provided quantitation method Kit (Biocrates Life Sciences AG, Innsbruck, Austria). 1 Siskos AP, Jain P, Römisch-Margl W, Bennett M, Achaintre D, Asad Y, et al. Interlaboratory Reproducibility of a Targeted Metabolomics Platform for Analysis of Human Serum and Plasma. Anal Chem 2017;89:656-65.
Sampleprep Protocol Filename:Sample_preparation.pdf

Combined analysis:

Analysis ID AN003722
Analysis type MS
Chromatography type Reversed phase
Chromatography system UPLC (Waters Acquity, Waters Corporation, Milford, USA)
Column Agilent Zorbax Eclipse XDB C18 (100 x 3.0mm,3.5um)
MS Type ESI
MS instrument type QTRAP
MS instrument name ABI Sciex 5500 QTrap
Ion Mode POSITIVE
Units µM

Chromatography:

Chromatography ID:CH002756
Chromatography Summary:LC - Instrument Parameters AbsoluteIDQ® p180 Kit (Biocrates Life Science AG, Innsbruck, Austria) System: UPLC (Waters Acquity, Waters Corporation, Milford, USA) Column: Agilent, Zorbax Eclipse XDB C18, 3.0 x 100 mm, 3.5 µM, Agilent Waldbronn, Germany Precolumn: Security Guard, Phenomenex, C18, 4 x 3 mm; Phenomenex, Aschaffenburg, Germany Materials: Water MiliQ, Methanol (Merck KGaA, Darmstadt, Germany, hypergrade for LC-MS) Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS) Ammonium Acetate (Honeywell - Fluka, Seelze, Germany) Formic Acid (Honeywell, Fluka, Seelze, Germany) Running Solvent: 5mM ammonium acetat in methanol FIA: B: Mix running solvent and MiliQ water 1:1 Gradient Table FIA Time (min) Flow Rate (mL/min) %A %B Curve Initial 0.030 0.0 100.0 Initial 1.60 0.030 0.0 100.0 6 2.40 0.200 0.0 100.0 6 2.80 0.200 0.0 100.0 6 3.00 0.030 0.0 100.0 6
Methods Filename:LC_parameters_metabolites.pdf
Instrument Name:UPLC (Waters Acquity, Waters Corporation, Milford, USA)
Column Name:Agilent Zorbax Eclipse XDB C18 (100 x 3.0mm,3.5um)
Flow Gradient:FIA Time (min) Flow Rate (mL/min) %A %B Curve Initial 0.030 0.0 100.0 Initial 1.60 0.030 0.0 100.0 6 2.40 0.200 0.0 100.0 6 2.80 0.200 0.0 100.0 6 3.00 0.030 0.0 100.0 6
Flow Rate:0.03ml/min
Solvent A:100% methanol; 5mM ammonium acetate
Solvent B:50% methanol/50% water; 5mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS003470
Analysis ID:AN003722
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:MS - Instrument Parameter AbsoluteIDQ® p180 Kit (Biocrates Life Science AG, Innsbruck, Austria) MS -Q-Trap 5500 Sciex Ion Source: Turbo Spray Curtian Gas: 20 CAD Gas: Medium Ion Spray Voltage: 5500 V Temperature: 500°C Ion Source Gas 1: 40psi Ion Source Gas 2: 50 psi
Ion Mode:POSITIVE
Analysis Protocol File:MS_metabolites.pdf
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