Summary of Study ST002347

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001507. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ53 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002347
Study TitleImpact of Visceral Leishmaniasis on Local Organ Metabolism in Hamsters
Study SummaryLeishmania is an intracellular parasite with different species pathogenic to humans and causing the disease leishmaniasis. Leishmania donovani causes visceral leishmaniasis (VL) that manifests as hepatosplenomegaly, fever, pancytopenia and hypergammaglobulinemia. If left without treatment, VL can cause death, especially in immunocompromised people. Current treatments have often significant adverse effects, and resistance has been reported in some countries. Determining the metabolites perturbed during VL can lead us to find new treatments targeting disease pathogenesis. We therefore compared metabolic perturbation between L. donovani-infected and uninfected hamsters across organs (spleen, liver, and gut). Metabolites were extracted, analyzed by liquid chromatography-mass spectrometry, and processed with MZmine and molecular networking to annotate metabolites. We found few metabolites commonly impacted by infection across all three sites, including glycerophospholipids, ceramides, acylcarnitines, peptides, purines and amino acids. In accordance with VL symptoms and parasite tropism, we found a greater overlap of perturbed metabolites between spleen and liver compared to spleen and gut, or liver and gut. Targeting pathways related to these metabolite families would be the next focus that can lead us to find more effective treatments for VL.
Institute
University of Oklahoma
Last NameMcCall
First NameLaura-Isobel
Address101 Stephenson Pkwy, Norman, OK, 73019-5251, USA
Emaillmccall@ou.edu
Phone4053259385
Submit Date2022-10-23
Num Groups2
Total Subjects14-16
Publicationshttps://doi.org/10.3390/metabo12090802
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-11-28
Release Version1
Laura-Isobel McCall Laura-Isobel McCall
https://dx.doi.org/10.21228/M8WQ53
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001507
Project DOI:doi: 10.21228/M8WQ53
Project Title:Impact of Visceral Leishmaniasis on Local Organ Metabolism in Hamsters
Project Summary:Leishmania is an intracellular parasite with different species pathogenic to humans and causing the disease leishmaniasis. Leishmania donovani causes visceral leishmaniasis (VL) that manifests as hepatosplenomegaly, fever, pancytopenia and hypergammaglobulinemia. If left without treatment, VL can cause death, especially in immunocompromised people. Current treatments have often significant adverse effects, and resistance has been reported in some countries. Determining the metabolites perturbed during VL can lead us to find new treatments targeting disease pathogenesis. We therefore compared metabolic perturbation between L. donovani-infected and uninfected hamsters across organs (spleen, liver, and gut). Metabolites were extracted, analyzed by liquid chromatography-mass spectrometry, and processed with MZmine and molecular networking to annotate metabolites. We found few metabolites commonly impacted by infection across all three sites, including glycerophospholipids, ceramides, acylcarnitines, peptides, purines and amino acids. In accordance with VL symptoms and parasite tropism, we found a greater overlap of perturbed metabolites between spleen and liver compared to spleen and gut, or liver and gut. Targeting pathways related to these metabolite families would be the next focus that can lead us to find more effective treatments for VL.
Institute:University of Oklahoma
Last Name:McCall
First Name:Laura-Isobel
Address:101 Stephenson Pkwy, Norman, OK, 73019-5251, USA
Email:lmccall@ou.edu
Phone:4053259385

Subject:

Subject ID:SU002436
Subject Type:Mammal
Subject Species:Mesocricetus auratus

Factors:

Subject type: Mammal; Subject species: Mesocricetus auratus (Factor headings shown in green)

mb_sample_id local_sample_id organ experiment group
SA235512X6.mix_20200814130541.mzXML6.mix 6.mix 6.mix
SA235513X6.mix.mzXML6.mix 6.mix 6.mix
SA235514X6.mix_20200814164217.mzXML6.mix 6.mix 6.mix
SA235529Start_blank_20200814125728.mzXMLblank blank blank
SA235530Start_blank_20200814150228.mzXMLblank blank blank
SA235531Start_blank_20200814163404.mzXMLblank blank blank
SA235532Start_blank_20200814110048.mzXMLblank blank blank
SA235533Start_blank_20200814011747.mzXMLblank blank blank
SA235534Start_blank_20200813204327.mzXMLblank blank blank
SA235535Start_blank.mzXMLblank blank blank
SA235536Start_blank_20200814090407.mzXMLblank blank blank
SA235537Start_blank_20200813232118.mzXMLblank blank blank
SA235538Start_blank_20200814070728.mzXMLblank blank blank
SA235539Start_blank_20200814051051.mzXMLblank blank blank
SA235540Start_blank_20200814031416.mzXMLblank blank blank
SA235541X1693_Ld1s_gut_P79.mzXMLgut AB4 Ld1s
SA235542X1695_Ld1s_gut_P79.mzXMLgut AB4 Ld1s
SA235543X1699_Ld1s_gut_P79.mzXMLgut AB4 Ld1s
SA235544X1692_Ld1s_gut_P79.mzXMLgut AB4 Ld1s
SA235545X1698_Ld1s_gut_P79.mzXMLgut AB4 Ld1s
SA235546X1694_Ld1s_gut_P79.mzXMLgut AB4 Ld1s
SA235547X1697_Ld1s_plus_ABX_gut_P79.mzXMLgut AB4 Ld1s_plus ABX
SA235548X1691_Ld1s_plus_ABX_gut_P79.mzXMLgut AB4 Ld1s_plus ABX
SA235549X1696_Ld1s_plus_ABX_gut_P79.mzXMLgut AB4 Ld1s_plus ABX
SA235550X1690_Ld1s_plus_ABX_gut_P79.mzXMLgut AB4 Ld1s_plus ABX
SA235551X16101_Ld1s_plus_ABX_gut_P80.mzXMLgut AB4 Ld1s_plus ABX
SA235552X16100_Ld1s_plus_ABX_gut_P80.mzXMLgut AB4 Ld1s_plus ABX
SA235553X1689_Naive_gut_P79.mzXMLgut AB4 Naive
SA235554X1688_Naive_gut_P79.mzXMLgut AB4 Naive
SA235555X1687_Naive_plus_ABX_gut_P79.mzXMLgut AB4 Naive_plus ABX
SA235556X1686_Naive_plus_ABX_gut_P79.mzXMLgut AB4 Naive_plus ABX
SA235557X1711_Ld1s_gut_P80.mzXMLgut AB5 Ld1s
SA235558X1703_Ld1s_gut_P79.mzXMLgut AB5 Ld1s
SA235559X1705_Ld1s_gut_P79.mzXMLgut AB5 Ld1s
SA235560X1712_Ld1s_gut_P80.mzXMLgut AB5 Ld1s
SA235561X1709_Ld1s_plus_ABX_gut_P80.mzXMLgut AB5 Ld1s_plus ABX
SA235562X1706_Ld1s_plus_ABX_gut_P79.mzXMLgut AB5 Ld1s_plus ABX
SA235563X1704_Ld1s_plus_ABX_gut_P79.mzXMLgut AB5 Ld1s_plus ABX
SA235564X1710_Ld1s_plus_ABX_gut_P80.mzXMLgut AB5 Ld1s_plus ABX
SA235565X1701_Naive_gut_P79.mzXMLgut AB5 Naive
SA235566X1702_Naive_gut_P79.mzXMLgut AB5 Naive
SA235567X1708_Naive_plus_ABX_gut_P79.mzXMLgut AB5 Naive_plus ABX
SA235568X1692_Ld1s_liver_P79.mzXMLliver AB4 Ld1s
SA235569X1694_Ld1s_liver_P79.mzXMLliver AB4 Ld1s
SA235570X1698_Ld1s_liver_P79.mzXMLliver AB4 Ld1s
SA235571X1695_Ld1s_liver_P79.mzXMLliver AB4 Ld1s
SA235572X1693_Ld1s_liver_P79.mzXMLliver AB4 Ld1s
SA235573X1699_Ld1s_liver_P79.mzXMLliver AB4 Ld1s
SA235574X16100_Ld1s_plus_ABX_liver_P80.mzXMLliver AB4 Ld1s_plus ABX
SA235575X1696_Ld1s_plus_ABX_liver_P79.mzXMLliver AB4 Ld1s_plus ABX
SA235576X1697_Ld1s_plus_ABX_liver_P79.mzXMLliver AB4 Ld1s_plus ABX
SA235577X1690_Ld1s_plus_ABX_liver_P79.mzXMLliver AB4 Ld1s_plus ABX
SA235578X1691_Ld1s_plus_ABX_liver_P79.mzXMLliver AB4 Ld1s_plus ABX
SA235579X1689_Naive_liver_P79.mzXMLliver AB4 Naive
SA235580X1688_Naive_liver_P79.mzXMLliver AB4 Naive
SA235581X1687_Naive_plus_ABX_liver_P79.mzXMLliver AB4 Naive_plus ABX
SA235582X1686_Naive_plus_ABX_liver_P79.mzXMLliver AB4 Naive_plus ABX
SA235583X1703_Ld1s_liver_P79.mzXMLliver AB5 Ld1s
SA235584X1712_Ld1s_liver_P80.mzXMLliver AB5 Ld1s
SA235585X1711_Ld1s_liver_P80.mzXMLliver AB5 Ld1s
SA235586X1705_Ld1s_liver_P79.mzXMLliver AB5 Ld1s
SA235587X1710_Ld1s_plus_ABX_liver_P80.mzXMLliver AB5 Ld1s_plus ABX
SA235588X1706_Ld1s_plus_ABX_liver_P79.mzXMLliver AB5 Ld1s_plus ABX
SA235589X1709_Ld1s_plus_ABX_liver_P80.mzXMLliver AB5 Ld1s_plus ABX
SA235590X1704_Ld1s_plus_ABX_liver_P79.mzXMLliver AB5 Ld1s_plus ABX
SA235591X1701_Naive_liver_P79.mzXMLliver AB5 Naive
SA235592X1702_Naive_liver_P79.mzXMLliver AB5 Naive
SA235593X1707_Naive_plus_ABX_liver_P79.mzXMLliver AB5 Naive_plus ABX
SA235594X1708_Naive_plus_ABX_liver_P79.mzXMLliver AB5 Naive_plus ABX
SA235515pos_pooled_QC3.mzXMLQC QC QC
SA235516pos_pooled_QC4.mzXMLQC QC QC
SA235517pos_pooled_QC1.mzXMLQC QC QC
SA235518pos_pooled_QC2_20200814131354.mzXMLQC QC QC
SA235519pos_pooled_QC5.mzXMLQC QC QC
SA235520pos_pooled_QC2_20200814151041.mzXMLQC QC QC
SA235521pos_pooled_QC2_20200814012600.mzXMLQC QC QC
SA235522pos_pooled_QC2_20200813232930.mzXMLQC QC QC
SA235523pos_pooled_QC2.mzXMLQC QC QC
SA235524pos_pooled_QC2_20200814051904.mzXMLQC QC QC
SA235525pos_pooled_QC2_20200814032229.mzXMLQC QC QC
SA235526pos_pooled_QC2_20200814091220.mzXMLQC QC QC
SA235527pos_pooled_QC2_20200814110901.mzXMLQC QC QC
SA235528pos_pooled_QC2_20200814071541.mzXMLQC QC QC
SA235595X1668_Ld1s_spleen_P79.mzXMLspleen AB3 Ld1s
SA235596X1669_Ld1s_spleen_P79.mzXMLspleen AB3 Ld1s
SA235597X1665_Ld1s_plus_ABX_spleen_P79.mzXMLspleen AB3 Ld1s_plus ABX
SA235598X1667_Ld1s_plus_ABX_spleen_P79.mzXMLspleen AB3 Ld1s_plus ABX
SA235599X1666_Ld1s_plus_ABX_spleen_P79.mzXMLspleen AB3 Ld1s_plus ABX
SA235600X1664_Ld1s_plus_ABX_spleen_P79.mzXMLspleen AB3 Ld1s_plus ABX
SA235601X1670_Ld1s_plus_ABX_spleen_P79.mzXMLspleen AB3 Ld1s_plus ABX
SA235602X1671_Naive_plus_ABX_spleen_P79.mzXMLspleen AB3 Naive_plus ABX
SA235603X1699_Ld1s_spleen_P79_2.mzXMLspleen AB4 Ld1s
SA235604X1699_Ld1s_spleen_P79_1.mzXMLspleen AB4 Ld1s
SA235605X1698_Ld1s_spleen_P79_2.mzXMLspleen AB4 Ld1s
SA235606X1694_Ld1s_spleen_P79_1.mzXMLspleen AB4 Ld1s
SA235607X1694_Ld1s_spleen_P79_2.mzXMLspleen AB4 Ld1s
SA235608X1693_Ld1s_spleen_P79_2.mzXMLspleen AB4 Ld1s
SA235609X1693_Ld1s_spleen_P79_1.mzXMLspleen AB4 Ld1s
SA235610X1698_Ld1s_spleen_P79_1.mzXMLspleen AB4 Ld1s
SA235611X1692_Ld1s_spleen_P79_2.mzXMLspleen AB4 Ld1s
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Collection:

Collection ID:CO002429
Collection Summary:Organs were collected and stored at -80.
Sample Type:Liver;Spleen;Gut

Treatment:

Treatment ID:TR002448
Treatment Summary:Hamsters were infected with Leishmania donovani or remained uninfected

Sample Preparation:

Sampleprep ID:SP002442
Sampleprep Summary:Metabolites were extracted through a two-step extraction process, first in 50% methanol and then in 3:1 dichloromethane-methanol

Combined analysis:

Analysis ID AN003832
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Phenomenex Kinetex C8 (50mm x 2.1mm,0.7um,100Å)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH002837
Instrument Name:Thermo Vanquish
Column Name:Phenomenex Kinetex C8 (50mm x 2.1mm,0.7um,100Å)
Chromatography Type:Reversed phase

MS:

MS ID:MS003574
Analysis ID:AN003832
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
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