Summary of Study ST002392

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001539. The data can be accessed directly via it's Project DOI: 10.21228/M8RT4H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002392
Study TitleCandida albicans NMR exometabolome
Study SummaryThe NMR-based metabolomics analysis of 95 C. albicans isolates using the culture supernatants collected after 14h of incubation. The processed data are used as binns or as annotated and quantified metabolites data for study of the isolates variability within and between genomic clades.
Institute
Bioaster
DepartmentOmics Hub
LaboratoryMetabolomics
Last NameBunescu
First NameAndrei
Address40, avenue Tony Garnier, Lyon, 69007
Emailandrei.bunescu@bioaster.org
Phone+33481110650
Submit Date2022-12-06
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2023-06-06
Release Version1
Andrei Bunescu Andrei Bunescu
https://dx.doi.org/10.21228/M8RT4H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001539
Project DOI:doi: 10.21228/M8RT4H
Project Title:Clade delineation and isolate-specific variability of Candida albicans exometabolome
Project Summary:The study includes the NMR-based metabolomics analysis of 95 C. albicans isolates from most of representative genomic clades including the so called clade 13, C. africana. The metabolomics study is based on the supernatants of C. albicans growth after 14h of incubation. All generated data were then quantified with the help of Chenomx NMR suite.
Institute:Bioaster
Department:Omics Hub
Laboratory:Metabolomics
Last Name:Bunescu
First Name:Andrei
Address:40, avenue Tony Garnier, Lyon, 69007
Email:andrei.bunescu@bioaster.org
Phone:+33 (0)4.81.11.06.50
Funding Source:We received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie action, Innovative Training Network: FunHoMic; grant N° 812969. BIOASTER received funding from the French Government ‘Investissement d'Avenir’ program (Grant No. ANR-10-AIRT-03).

Subject:

Subject ID:SU002481
Subject Type:Yeast
Subject Species:Candida albicans
Species Group:Yeast

Factors:

Subject type: Yeast; Subject species: Candida albicans (Factor headings shown in green)

mb_sample_id local_sample_id Conditions Type Media Cluster Country Site Provenance PreCultureMorphology
SA238197YPD B_12 Mediablank Blank YPDbroth Control - - - -
SA238198YPD A_09Mediablank Blank YPDbroth Control - - - -
SA238199YPD A_05Mediablank Blank YPDbroth Control - - - -
SA238200YPD A_11Mediablank Blank YPDbroth Control - - - -
SA238201YPD B_10Mediablank Blank YPDbroth Control - - - -
SA238202YPD B_04Mediablank Blank YPDbroth Control - - - -
SA238203YPD A_03Mediablank Blank YPDbroth Control - - - -
SA238204YPD B_06Mediablank Blank YPDbroth Control - - - -
SA238205YPD B_02Mediablank Blank YPDbroth Control - - - -
SA238206YPD A_01Mediablank Blank YPDbroth Control - - - -
SA238207YPD B_08Mediablank Blank YPDbroth Control - - - -
SA238208YPD B_14Mediablank Blank YPDbroth Control - - - -
SA238209YPD A_07Mediablank Blank YPDbroth Control - - - -
SA238210YPD A_13Mediablank Blank YPDbroth Control - - - -
SA238211succinate B_10Succinatestandard Standard water Blank - - - -
SA238212succinate A_03Succinatestandard Standard water Blank - - - -
SA238213succinate B_08Succinatestandard Standard water Blank - - - -
SA238214succinate A_13Succinatestandard Standard water Blank - - - -
SA238215succinate A_09Succinatestandard Standard water Blank - - - -
SA238216succinate B_12Succinatestandard Standard water Blank - - - -
SA238217succinate B_06Succinatestandard Standard water Blank - - - -
SA238218succinate A_07Succinatestandard Standard water Blank - - - -
SA238219succinate B_01Succinatestandard Standard water Blank - - - -
SA238220succinate A_11Succinatestandard Standard water Blank - - - -
SA238221succinate B_14Succinatestandard Standard water Blank - - - -
SA238222succinate A_01Succinatestandard Standard water Blank - - - -
SA238223succinate A_05Succinatestandard Standard water Blank - - - -
SA238224succinate A_04Succinatestandard Standard water Blank - - - -
SA238225CEC3548_03Supernatantsample Sample YPDbroth A Belgium commensal Human no slide
SA238226CEC3548_04Supernatantsample Sample YPDbroth A Belgium commensal Human no slide
SA238227CEC3548_02Supernatantsample Sample YPDbroth A Belgium commensal Human yeast
SA238228CEC3548_01Supernatantsample Sample YPDbroth A Belgium commensal Human yeast
SA238229CEC3548_05Supernatantsample Sample YPDbroth A Belgium commensal Human yeast
SA238230CEC3548_06Supernatantsample Sample YPDbroth A Belgium commensal Human yeast
SA238231CEC3715_04Supernatantsample Sample YPDbroth A France superficial Human no slide
SA238232CEC3715_03Supernatantsample Sample YPDbroth A France superficial Human no slide
SA238233CEC3715_05Supernatantsample Sample YPDbroth A France superficial Human yeast
SA238234CEC3715_02Supernatantsample Sample YPDbroth A France superficial Human yeast
SA238235CEC3715_01Supernatantsample Sample YPDbroth A France superficial Human yeast
SA238236CEC3715_06Supernatantsample Sample YPDbroth A France superficial Human yeast
SA238237CEC4038_03Supernatantsample Sample YPDbroth A UK superficial Human long pseudohyphae
SA238238CEC4038_04Supernatantsample Sample YPDbroth A UK superficial Human long pseudohyphae
SA238239CEC4038_06Supernatantsample Sample YPDbroth A UK superficial Human no slide
SA238240CEC4038_05Supernatantsample Sample YPDbroth A UK superficial Human no slide
SA238241CEC4038_01Supernatantsample Sample YPDbroth A UK superficial Human yeast
SA238242CEC4038_02Supernatantsample Sample YPDbroth A UK superficial Human yeast
SA238243CEC4254_02Supernatantsample Sample YPDbroth B France invasive Human yeast
SA238244CEC4254_01Supernatantsample Sample YPDbroth B France invasive Human yeast
SA238245CEC4254_03Supernatantsample Sample YPDbroth B France invasive Human yeast
SA238246CEC4254_05Supernatantsample Sample YPDbroth B France invasive Human yeast
SA238247CEC4254_04Supernatantsample Sample YPDbroth B France invasive Human yeast
SA238248CEC4254_06Supernatantsample Sample YPDbroth B France invasive Human yeast
SA238249CEC3708_06Supernatantsample Sample YPDbroth B France superficial Human yeast
SA238250CEC3708_02Supernatantsample Sample YPDbroth B France superficial Human yeast
SA238251CEC3708_04Supernatantsample Sample YPDbroth B France superficial Human yeast
SA238252CEC3708_05Supernatantsample Sample YPDbroth B France superficial Human yeast
SA238253CEC3708_01Supernatantsample Sample YPDbroth B France superficial Human yeast
SA238254CEC3708_03Supernatantsample Sample YPDbroth B France superficial Human yeast
SA238269CEC3672_01Supernatantsample Sample YPDbroth C01 France invasive Human few hyphae
SA238270CEC3672_02Supernatantsample Sample YPDbroth C01 France invasive Human few hyphae
SA238271CEC3672_06Supernatantsample Sample YPDbroth C01 France invasive Human no slide
SA238272CEC3672_05Supernatantsample Sample YPDbroth C01 France invasive Human no slide
SA238273CEC3672_03Supernatantsample Sample YPDbroth C01 France invasive Human yeast
SA238274CEC3672_04Supernatantsample Sample YPDbroth C01 France invasive Human yeast
SA238275CEC3678_03Supernatantsample Sample YPDbroth C01 France superficial Human no slide
SA238276CEC4512_03Supernatantsample Sample YPDbroth C01 France superficial Human no slide
SA238277CEC4512_04Supernatantsample Sample YPDbroth C01 France superficial Human no slide
SA238278CEC3678_04Supernatantsample Sample YPDbroth C01 France superficial Human no slide
SA238279CEC4512_06Supernatantsample Sample YPDbroth C01 France superficial Human short pseudohyphae
SA238280CEC4512_05Supernatantsample Sample YPDbroth C01 France superficial Human short pseudohyphae
SA238281CEC4256_05Supernatantsample Sample YPDbroth C01 France superficial Human short pseudohyphae/hyphae
SA238282CEC4256_06Supernatantsample Sample YPDbroth C01 France superficial Human short pseudohyphae/hyphae
SA238283CEC3660_04Supernatantsample Sample YPDbroth C01 France superficial Human with long pseudohyphae
SA238284CEC3660_03Supernatantsample Sample YPDbroth C01 France superficial Human with long pseudohyphae
SA238285CEC3678_06Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238286CEC3660_01Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238287CEC4512_02Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238288CEC3679_04Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238289CEC4256_02Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238290CEC4256_01Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238291CEC3660_02Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238292CEC3660_06Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238293CEC3660_05Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238294CEC3679_01Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238295CEC3678_02Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238296CEC3678_01Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238297CEC3679_02Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238298CEC4256_04Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238299CEC3679_03Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238300CEC4256_03Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238301CEC4512_01Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238302CEC3679_05Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238303CEC3678_05Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238304CEC3679_06Supernatantsample Sample YPDbroth C01 France superficial Human yeast
SA238305CEC3534_05Supernatantsample Sample YPDbroth C01 Morocco commensal Human no slide
SA238306CEC3534_06Supernatantsample Sample YPDbroth C01 Morocco commensal Human no slide
SA238307CEC3534_03Supernatantsample Sample YPDbroth C01 Morocco commensal Human yeast
SA238308CEC3534_04Supernatantsample Sample YPDbroth C01 Morocco commensal Human yeast
SA238309CEC3534_01Supernatantsample Sample YPDbroth C01 Morocco commensal Human yeast
SA238310CEC3534_02Supernatantsample Sample YPDbroth C01 Morocco commensal Human yeast
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Collection:

Collection ID:CO002474
Collection Summary:The 96 clinical C. albicans isolates from the Fungal Biology and Pathogenecity Unit of the Institut Pasteur(Paris, France).
Sample Type:Yeast cells
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002493
Treatment Summary:No treatement was applied during the yeast culture. The growth of C. albicans isoltes were conducted in YPD media.

Sample Preparation:

Sampleprep ID:SP002487
Sampleprep Summary:The C.albicans isolates were grown in deep-weel plates with initial inoculum of 0.2 andin YPD growth media.

Analysis:

Analysis ID:AN003897
Laboratory Name:Omics
Analysis Type:NMR
Operator Name:Andrei Bunescu, ALABAN Leovigildo-Rey
Data Format:FID
Num Factors:150
Num Metabolites:40
Units:µM

NMR:

NMR ID:NM000257
Analysis ID:AN003897
Instrument Name:Ascend 600MHz Avance III HD
Instrument Type:FT-NMR
NMR Experiment Type:1D-1H
Spectrometer Frequency:600MHz
NMR Probe:QCI 1H/13C/15N/31P
NMR Solvent:H2O D2O
NMR Tube Size:3mm
Shimming Method:topshim and topshim 3d
Pulse Sequence:noesygppr1d
Water Suppression:presaturation
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