Summary of Study ST002824

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001766. The data can be accessed directly via it's Project DOI: 10.21228/M8FB1R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002824
Study TitleLeukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia
Study SummaryWe performed metabolomic analysis to measure polar and lipid metabolites in the serum from TgLC and TgL mice with Tamoxifen (TAM) injection. Mice under both fed and fasted conditions were included.
Institute
Rutgers University
Last NameHu
First NameWenwei
Address195 Little Albany St, New Brunswick, NJ 08901
Emailwh221@cinj.rutgers.edu
Phone7322356169
Submit Date2023-08-18
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-11-29
Release Version1
Wenwei Hu Wenwei Hu
https://dx.doi.org/10.21228/M8FB1R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001766
Project DOI:doi: 10.21228/M8FB1R
Project Title:Leukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia
Project Summary:We performed metabolomic analysis to measure polar and lipid metabolites in the serum from TgLC and TgL mice with Tamoxifen (TAM) injection. Mice under both fed and fasted conditions were included.
Institute:Rutgers University
Department:Department of Radiation Oncology
Last Name:Hu
First Name:Wenwei
Address:195 Little Albany St, New Brunswick, NJ 08901
Email:wh221@cinj.rutgers.edu
Phone:7322356169

Subject:

Subject ID:SU002933
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Condition Metabolites
SA305620TgLIF_S_2FTgLC Fasted lipid
SA305621TgLIF_S_2MTgLC Fasted lipid
SA305622TgLIF_S_1FTgLC Fasted lipid
SA305623TgLIF_S_1MTgLC Fasted lipid
SA305624Serum_Tglif-S_3mTgLC Fasted polar
SA305625Serum_Tglif-S_2mTgLC Fasted polar
SA305626Serum_Tglif-S_2fTgLC Fasted polar
SA305627Serum_Tglif-S_1mTgLC Fasted polar
SA305628TgLIF_F_2MTgLC Fed lipid
SA305629TgLIF_F_1MTgLC Fed lipid
SA305630TgLIF_F_1FTgLC Fed lipid
SA305631TgLIF_F_2FTgLC Fed lipid
SA305632Serum_Tglif-F_1fTgLC Fed polar
SA305633Serum_Tglif-F_2fTgLC Fed polar
SA305634Serum_Tglif-F_1mTgLC Fed polar
SA305635Serum_Tglif-F_2mTgLC Fed polar
SA305636WT_S_1MTgL Fasted lipid
SA305637WT_S_2MTgL Fasted lipid
SA305638WT_S_1FTgL Fasted lipid
SA305639WT_S_2FTgL Fasted lipid
SA305640Serum_WT-S_1fTgL Fasted polar
SA305641Serum_WT-S_2fTgL Fasted polar
SA305642Serum_WT-S_3mTgL Fasted polar
SA305643Serum_WT-S_3fTgL Fasted polar
SA305644WT_F_1FTgL Fed lipid
SA305645WT_F_2MTgL Fed lipid
SA305646WT_F_1MTgL Fed lipid
SA305647WT_F_2FTgL Fed lipid
SA305648Serum_WT-F_1fTgL Fed polar
SA305649Serum_WT-F_2fTgL Fed polar
SA305650Serum_WT-F_1mTgL Fed polar
SA305651Serum_WT-F_2mTgL Fed polar
Showing results 1 to 32 of 32

Collection:

Collection ID:CO002926
Collection Summary:Whole blood was collected and centrifuged at 9000 rpm for 10 minutes. The supernatant is serum. Serum was then stored at -80 °C.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR002942
Treatment Summary:TgL and TgLC mice were injected with Tamoxifen under the fed and fasted conditions.

Sample Preparation:

Sampleprep ID:SP002939
Sampleprep Summary:For lipidomic metabolites extraction, 10 μL serum were mixed with 75 μL methanol and 250 μL Methyl tert-butyl ether (MTBE). After shaking for 6 minutes at 4 °C, 62 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further analysis. Polar metabolites were extracted from serum using the extraction buffer containing methanol: acetonitrile: H2O (40:40:20).

Combined analysis:

Analysis ID AN004605 AN004606 AN004607 AN004608
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish Thermo Vanquish Thermo Vanquish
Column Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um) Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Ion Mode NEGATIVE POSITIVE NEGATIVE POSITIVE
Units Ion counts Ion counts Ion counts Ion counts

Chromatography:

Chromatography ID:CH003465
Chromatography Summary:Polar metabolite analysis
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:25C
Flow Gradient:0 min, 100% B; 3 min, 100% B; 3.2 min, 90% B; 6.2 min, 90% B; 6.5 min, 80% B; 10.5 min, 80% B; 10.7 min, 70% B; 13.5 min, 70% B; 13.7 min, 45% B; 16 min, 45% B; 16.5 min, 100% B; and 22 min, 100% B
Flow Rate:0.3mL/min
Solvent A:95% water/5% acetonitrile; 20 mM acetic acid; 40 mM ammonium hydroxide, pH 9.4
Solvent B:20% water/80% acetonitrile; 20 mM acetic acid; 40 mM ammonium hydroxide, pH 9.4
Chromatography Type:HILIC
  
Chromatography ID:CH003466
Chromatography Summary:Lipidomics method
Instrument Name:Thermo Vanquish
Column Name:Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
Column Temperature:55C
Flow Gradient:0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B
Flow Rate:0.2mL/min
Solvent A:90% water/10% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate
Solvent B:75% isopropanol/25% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS004351
Analysis ID:AN004605
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Negative Ionization mode
Ion Mode:NEGATIVE
Capillary Temperature:300
Ion Source Temperature:360
Spray Voltage:2700
Automatic Gain Control:3000000
Acquisition Parameters File:LCMS_Neg_22min_B_Vanquish
  
MS ID:MS004352
Analysis ID:AN004606
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Positive Ionization mode
Ion Mode:POSITIVE
Capillary Temperature:300
Ion Source Temperature:360
Spray Voltage:3500
Automatic Gain Control:3000000
Acquisition Parameters File:LCMS_Pos_22min_B_Vanquish
  
MS ID:MS004353
Analysis ID:AN004607
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Negative Ionization mode
Ion Mode:NEGATIVE
Capillary Temperature:300
Ion Source Temperature:360
Spray Voltage:2700
Automatic Gain Control:3000000
Acquisition Parameters File:LCMS_Neg_30min_55C_LongElution
  
MS ID:MS004354
Analysis ID:AN004608
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Positive Ionization mode
Ion Mode:POSITIVE
Capillary Temperature:300
Ion Source Temperature:360
Spray Voltage:3500
Automatic Gain Control:3000000
Acquisition Parameters File:LCMS_Pos_30min_55C_LongElution
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