Summary of Study ST002843

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001766. The data can be accessed directly via it's Project DOI: 10.21228/M8FB1R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002843
Study TitleLeukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia (Tamoxifen)
Study SummaryWe compared the levels of lipid metabolites in the liver from TgLC and TgL mice with Tamoxifen injection. The lipid metabolites levels in the liver from Balb/c mice bearing C26 or C26-LIF KO tumors were also measured. Mice under both fed and fasted conditions were included.
Institute
Rutgers University
Last NameHu
First NameWenwei
Address195 Little Albany St, New Brunswick, NJ 08901
Emailwh221@cinj.rutgers.edu
Phone7322356169
Submit Date2023-08-23
Study CommentsSame project as Study:ST002824
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-11-29
Release Version1
Wenwei Hu Wenwei Hu
https://dx.doi.org/10.21228/M8FB1R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001766
Project DOI:doi: 10.21228/M8FB1R
Project Title:Leukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia
Project Summary:We performed metabolomic analysis to measure polar and lipid metabolites in the serum from TgLC and TgL mice with Tamoxifen (TAM) injection. Mice under both fed and fasted conditions were included.
Institute:Rutgers University
Department:Department of Radiation Oncology
Last Name:Hu
First Name:Wenwei
Address:195 Little Albany St, New Brunswick, NJ 08901
Email:wh221@cinj.rutgers.edu
Phone:7322356169

Subject:

Subject ID:SU002953
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Condition
SA307573c26_S_1C26 Fasted
SA307574c26_S_2C26 Fasted
SA307575c26_S_3C26 Fasted
SA307576c26_S_8C26 Fasted
SA307577c26_S_9C26 Fasted
SA307578c26_S_7C26 Fasted
SA307579c26_S_10C26 Fasted
SA307580c26_S_4C26 Fasted
SA307581c26_S_5C26 Fasted
SA307582c26_S_6C26 Fasted
SA307583c26_F_5C26 Fed
SA307584c26_F_3C26 Fed
SA307585c26_F_6C26 Fed
SA307586c26_F_4C26 Fed
SA307587c26_F_9C26 Fed
SA307588c26_F_2C26 Fed
SA307589c26_F_10C26 Fed
SA307590c26_F_8C26 Fed
SA307591c26_F_7C26 Fed
SA307592c26_F_1C26 Fed
SA307561c26-lifKO_S_3C26-LIF KO Fasted
SA307562c26-lifKO_S_2C26-LIF KO Fasted
SA307563c26-lifKO_S_4C26-LIF KO Fasted
SA307564c26-lifKO_S_1C26-LIF KO Fasted
SA307565c26-lifKO_S_5C26-LIF KO Fasted
SA307566c26-lifKO_S_6C26-LIF KO Fasted
SA307567c26-lifKO_F_5C26-LIF KO Fed
SA307568c26-lifKO_F_6C26-LIF KO Fed
SA307569c26-lifKO_F_4C26-LIF KO Fed
SA307570c26-lifKO_F_2C26-LIF KO Fed
SA307571c26-lifKO_F_3C26-LIF KO Fed
SA307572c26-lifKO_F_1C26-LIF KO Fed
SA307593NC-c26_S_3Con Fasted
SA307594NC-c26_S_4Con Fasted
SA307595NC-c26_S_5Con Fasted
SA307596NC-c26_S_6Con Fasted
SA307597NC-c26_S_2Con Fasted
SA307598NC-c26_S_1Con Fasted
SA307599NC-c26_F_6Con Fed
SA307600NC-c26_F_1Con Fed
SA307601NC-c26_F_4Con Fed
SA307602NC-c26_F_3Con Fed
SA307603NC-c26_F_2Con Fed
SA307604NC-c26_F_5Con Fed
SA307605Liver_Tglif_S_5MTgLC Fasted
SA307606Liver_Tglif_S_6FTgLC Fasted
SA307607Liver_Tglif_S_5FTgLC Fasted
SA307608Liver_Tglif_S_4FTgLC Fasted
SA307609Liver_Tglif_S_6MTgLC Fasted
SA307610Liver_Tglif_S_2FTgLC Fasted
SA307611Liver_Tglif_S_1FTgLC Fasted
SA307612Liver_Tglif_S_3FTgLC Fasted
SA307613Liver_Tglif_F_3MTgLC Fed
SA307614Liver_Tglif_F_5FTgLC Fed
SA307615Liver_Tglif_F_2FTgLC Fed
SA307616Liver_Tglif_F_1FTgLC Fed
SA307617Liver_Tglif_F_3FTgLC Fed
SA307618Liver_Tglif_F_4FTgLC Fed
SA307619Liver_Tglif_F_4MTgLC Fed
SA307620Liver_Tglif_F_6FTgLC Fed
SA307621Liver_WT_S_2FTgL Fasted
SA307622Liver_WT_S_4MTgL Fasted
SA307623Liver_WT_S_5MTgL Fasted
SA307624Liver_WT_S_1FTgL Fasted
SA307625Liver_WT_S_3MTgL Fasted
SA307626Liver_WT_S_6MTgL Fasted
SA307627Liver_WT_S_4FTgL Fasted
SA307628Liver_WT_S_3FTgL Fasted
SA307629Liver_WT_F_6FTgL Fed
SA307630Liver_WT_F_2FTgL Fed
SA307631Liver_WT_F_3MTgL Fed
SA307632Liver_WT_F_5FTgL Fed
SA307633Liver_WT_F_5MTgL Fed
SA307634Liver_WT_F_1FTgL Fed
SA307635Liver_WT_F_6MTgL Fed
SA307636Liver_WT_F_4MTgL Fed
Showing results 1 to 76 of 76

Collection:

Collection ID:CO002946
Collection Summary:TgLC and TgL mice were injected with TAM. Balb/c mice were injected with C26 or C26-LIF KO cells. Liver was collected and then stored at -80 °C.
Sample Type:Liver

Treatment:

Treatment ID:TR002962
Treatment Summary:TgL and TgLC mice were injected with Tamoxifen. Balb/c mice were injected with C26 or C26-LIF KO cells.

Sample Preparation:

Sampleprep ID:SP002959
Sampleprep Summary:For lipidomic metabolites extraction, 10 mg homogenized tissues were mixed with 120 μL methanol and 400 μL MTBE. After shaking for 6 minutes at 4 °C, 100 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further lipidomics analysis.

Combined analysis:

Analysis ID AN004652 AN004653
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE POSITIVE
Units Ion counts Ion counts

Chromatography:

Chromatography ID:CH003502
Chromatography Summary:Lipidomics method
Instrument Name:Thermo Vanquish
Column Name:Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
Column Temperature:55C
Flow Gradient:0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B
Flow Rate:0.2mL/min
Solvent A:90% water/10% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate
Solvent B:75% isopropanol/25% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS004399
Analysis ID:AN004652
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Positive Ionization mode
Ion Mode:POSITIVE
Capillary Temperature:300
Ion Source Temperature:360
Spray Voltage:2700
Automatic Gain Control:3000000
Acquisition Parameters File:LCMS_Pos_RT_30min_55C_LongElution
  
MS ID:MS004400
Analysis ID:AN004653
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Positive Ionization mode
Ion Mode:POSITIVE
Capillary Temperature:300
Ion Source Temperature:360
Spray Voltage:2700
Automatic Gain Control:3000000
Acquisition Parameters File:LCMS_Pos_RT_30min_55C_LongElution
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