Summary of Study ST002844
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001766. The data can be accessed directly via it's Project DOI: 10.21228/M8FB1R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002844 |
| Study Title | Identification of fatty acids which form triglycerides in TgLC mouse serum |
| Study Summary | We found significant changes in lipid metabolites, notably triglycerides (TGs), were observed in TgLC mice with TAM injection compared with TgL mice with TAM injection under both the fed and fasted conditions. To further identify the fatty acids which form TGs, we performed MS2 to do lipid annotation. |
| Institute | Rutgers University |
| Last Name | Hu |
| First Name | Wenwei |
| Address | 195 Little Albany St, New Brunswick, NJ 08901 |
| wh221@cinj.rutgers.edu | |
| Phone | 7322356169 |
| Submit Date | 2023-08-30 |
| Study Comments | study under projectID: PR001766 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-11-29 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR001766 |
| Project DOI: | doi: 10.21228/M8FB1R |
| Project Title: | Leukemia inhibitory factor suppresses hepatic de novo lipogenesis and induces cachexia |
| Project Summary: | We performed metabolomic analysis to measure polar and lipid metabolites in the serum from TgLC and TgL mice with Tamoxifen (TAM) injection. Mice under both fed and fasted conditions were included. |
| Institute: | Rutgers University |
| Department: | Department of Radiation Oncology |
| Last Name: | Hu |
| First Name: | Wenwei |
| Address: | 195 Little Albany St, New Brunswick, NJ 08901 |
| Email: | wh221@cinj.rutgers.edu |
| Phone: | 7322356169 |
Subject:
| Subject ID: | SU002955 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA308217 | WT_ddMS2 | TgL |
| SA308218 | LIF_ddMS2 | TgLC |
| Showing results 1 to 2 of 2 |
Collection:
| Collection ID: | CO002948 |
| Collection Summary: | Whole blood was collected and centrifuged at 9000 rpm for 10 minutes. The supernatant is serum. Serum was then stored at -80 °C. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR002964 |
| Treatment Summary: | TgL and TgLC mice were injected with Tamoxifen under the fed and fasted conditions. |
Sample Preparation:
| Sampleprep ID: | SP002961 |
| Sampleprep Summary: | For lipidomic metabolites extraction, 10 μL serum were mixed with 75 μL methanol and 250 μL Methyl tert-butyl ether (MTBE). After shaking for 6 minutes at 4 °C, 62 μL H2O was added and followed by centrifugation for 2 minutes. Supernatants were dried down for further analysis. |
Combined analysis:
| Analysis ID | AN004658 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish Horizon |
| Column | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | Ion counts |
Chromatography:
| Chromatography ID: | CH003507 |
| Chromatography Summary: | Lipidomics method (Vanquish Horizon) |
| Instrument Name: | Thermo Vanquish Horizon |
| Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um) |
| Column Temperature: | 55C |
| Flow Gradient: | 0 min, 25% B; 2 min, 25% B; 5.5 min, 65% B; 12.5 min, 100% B; 19.5 min, 100% B; 20.0 min, 25% B; 30 min, 25% B |
| Flow Rate: | 0.2mL/min |
| Solvent A: | 90% water/10% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate |
| Solvent B: | 75% isopropanol/25% methanol; 34.2 mM acetic acid; 1 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004405 |
| Analysis ID: | AN004658 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Positive Ionization mode with ddMS2 |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 300 |
| Ion Source Temperature: | 360 |
| Spray Voltage: | 3500 |
| Automatic Gain Control: | 3000000 |
