Summary of Study ST003273
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002030. The data can be accessed directly via it's Project DOI: 10.21228/M86V58 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003273 |
Study Title | Impact of serine supplementation following treatment with serine/glycine-depleted diet: Plasma |
Study Summary | We analyzed metabolites in the retina, choroid/RPE, plasma, and paw skin from mice that were previously on control or serine/glycine-depleted diet and then switched over to a control or serine-supplemented diet. This study contains plasma samples. |
Institute | Salk Institute for Biological Studies |
Department | Molecular and Cell Biology Laboratory |
Laboratory | Metallo Lab |
Last Name | Lim |
First Name | Esther |
Address | 10010 N Torrey Pines Rd |
ewlim2024@gmail.com | |
Phone | (858) 453-4100 |
Submit Date | 2024-06-15 |
Num Groups | 4 |
Total Subjects | 20 |
Num Males | 20 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2024-07-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR002030 |
Project DOI: | doi: 10.21228/M86V58 |
Project Title: | Metabolomic changes in mouse tissues following modulation of serine through the diet |
Project Summary: | We analyzed metabolites in the retina, choroid/RPE, and plasma from WT and PHGDH heterozygous mice that were fed either a control or serine/glycine-deprived diet. Additionally, we also analyzed metabolites from the retina, choroid/RPE, paw skin, and plasma from mice that were previously on either a control or serine/glycine-deprived diet and then switched back to a control or serine-supplemented diet. |
Institute: | Salk Institute |
Department: | Molecular and Cell Biology Laboratory |
Laboratory: | Metallo Lab |
Last Name: | Lim |
First Name: | Esther |
Address: | 10010 N Torrey Pines Rd, La Jolla, CA, 92037, USA |
Email: | ewlim2024@gmail.com |
Phone: | (858) 453-4100 |
Funding Source: | R01CA234245 |
Subject:
Subject ID: | SU003393 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 12-16 months |
Weight Or Weight Range: | 25-45 g |
Gender: | Male |
Animal Animal Supplier: | The Scripps Research Institute Vivarium |
Animal Housing: | 5/cage |
Animal Light Cycle: | 12h dark/12 h light |
Animal Feed: | Special diets; see group labeling |
Animal Water: | Ad libitum |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment | Sample source |
---|---|---|---|
SA354479 | LMRI_DietRev_plasma_20J5 | Plasma Control rescue | Mouse plasma |
SA354480 | LMRI_DietRev_plasma_20J4 | Plasma Control rescue | Mouse plasma |
SA354481 | LMRI_DietRev_plasma_20J3 | Plasma Control rescue | Mouse plasma |
SA354482 | LMRI_DietRev_plasma_20J2 | Plasma Control rescue | Mouse plasma |
SA354483 | LMRI_DietRev_plasma_20J1 | Plasma Control rescue | Mouse plasma |
SA354489 | LMRI_DietRev_plasma_20B3 | Plasma control | Mouse plasma |
SA354490 | LMRI_DietRev_plasma_20C3 | Plasma control | Mouse plasma |
SA354491 | LMRI_DietRev_plasma_20C2 | Plasma control | Mouse plasma |
SA354492 | LMRI_DietRev_plasma_20B4 | Plasma control | Mouse plasma |
SA354493 | LMRI_DietRev_plasma_20B2 | Plasma control | Mouse plasma |
SA354484 | LMRI_DietRev_plasma_20H5 | Plasma High serine rescue | Mouse plasma |
SA354485 | LMRI_DietRev_plasma_20H1 | Plasma High serine rescue | Mouse plasma |
SA354486 | LMRI_DietRev_plasma_20H4 | Plasma High serine rescue | Mouse plasma |
SA354487 | LMRI_DietRev_plasma_20H3 | Plasma High serine rescue | Mouse plasma |
SA354488 | LMRI_DietRev_plasma_20H2 | Plasma High serine rescue | Mouse plasma |
SA354474 | LMRI_DietRev_plasma_20D1 | Plasma -SG diet | Mouse plasma |
SA354475 | LMRI_DietRev_plasma_20D2 | Plasma -SG diet | Mouse plasma |
SA354476 | LMRI_DietRev_plasma_20D3 | Plasma -SG diet | Mouse plasma |
SA354477 | LMRI_DietRev_plasma_20D4 | Plasma -SG diet | Mouse plasma |
SA354478 | LMRI_DietRev_plasma_20D5 | Plasma -SG diet | Mouse plasma |
Showing results 1 to 20 of 20 |
Collection:
Collection ID: | CO003386 |
Collection Summary: | Blood samples collected into EDTA-coated microvette tubes. EDTA microvettes were spun at 2,000g at 4 °C for 5 min to obtain plasma, and samples stored at −80°C until analysis. |
Sample Type: | Blood (plasma) |
Treatment:
Treatment ID: | TR003402 |
Treatment Summary: | Mice were fed control or serine/glycine-depleted (-SG) diet for 12 months. After 12 months, a subset of the mice that were on the -SG diet were switched back to either the control or supplemented diet for 4 months. |
Sample Preparation:
Sampleprep ID: | SP003400 |
Sampleprep Summary: | For sphingolipid extraction from retina and choroid/RPE, frozen tissue was homogenized with a ball mill (Retsch Mixer Mill MM 400) at 30 Hz for 3 min in 500 μl of −20°C methanol, 400 μL of ice-cold saline, and 100 μL of ice-cold MilliQ water, spiked with deuterated internal standards as described earlier. The mixture was then transferred into a 2-ml Eppendorf tube containing 1 ml of chloroform. The tubes were vortexed for 5 min and centrifuged at 16,000g at 4°C for 5 min. The lower organic phase was collected, and 2 μL of formic acid was added to the remaining polar phase, which was re-extracted with 1 ml of chloroform. Combined organic phases were dried and resuspended in 50 μl of 0.2% formic acid and 1 mM ammonium formate in methanol. Last, the tubes were sonicated in a bath sonicator for 10 min and spun at 16,000g for 10 min at 4°C. For plasma, 50 μL of plasma was extracted with 500 μl of −20°C methanol, 400 μL of saline, and 100 μl of water spiked with deuterated internal standards. Chloroform (1 mL) was then added to the tubes. The tubes were vortexed for 5 min and centrifuged at 16,000g at 4°C for 5 min. The lower organic phase was collected, and 2 μl of formic acid was added to the remaining polar phase, which was reextracted with 1 ml of chloroform. Combined organic phases were dried and resuspended in 100 μl of 0.2% formic acid and 1 mM ammonium formate in methanol. Next, the tubes were sonicated in a bath sonicator for 10 min and spun at 16,000g for 10 min at 4°C |
Processing Storage Conditions: | Room temperature |
Extract Storage: | 4℃ |
Combined analysis:
Analysis ID | AN005359 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 6460 |
Column | Peeke Scientific Spectra C8SR (150 x 3.0 mm, 3μm) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6460 QQQ |
Ion Mode | POSITIVE |
Units | Normalized ion count/L |
Chromatography:
Chromatography ID: | CH004060 |
Chromatography Summary: | The gradient elution program consisted of the following profile: 0 min, 82% B; 3 min, 82% B; 4 min, 90% B, 18 min, 99% B; 25 min, 99%, 27 min, 82% B, 30 min, 82% B. |
Instrument Name: | Agilent 6460 |
Column Name: | Peeke Scientific Spectra C8SR (150 x 3.0 mm, 3μm) |
Column Temperature: | 40℃ |
Flow Gradient: | 0 min, 82% B; 3 min, 82% B; 4 min, 90% B; 18 min, 99% B; 25 min, 99% B; 27 min, 82% B; and 30 min, 82% B |
Flow Rate: | 0.5 mL/min |
Solvent A: | 100% water; 0.2% formic acid; 2 mM ammonium formate |
Solvent B: | 100% methanol; 0.2% formic acid; 1 mM ammonium formate |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS005089 |
Analysis ID: | AN005359 |
Instrument Name: | Agilent 6460 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Software: Agilent MassHunter Sphingolipid species were analyzed by selective reaction monitoring of the transition from precursor to product ions at associated optimized collision energies, and fragmentor voltages are provided elsewhere (https://doi.org/10.1007/978-1-60761-322-0_22). The m/z values of the precursor and product ions are provided in the metabolite metadata section. |
Ion Mode: | POSITIVE |