Summary of Study ST003348

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002083. The data can be accessed directly via it's Project DOI: 10.21228/M8C802 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003348
Study TitleAn integrated LC-MS analysis of the biometric characteristics of different time cohorts of race walkers - untargeted
Study SummaryTo obtain metabolic changes during endurance exercise, we conducted a metabolomics study in which 19 race walkers were recruited to collect blood at four time points: before, during, after, and after rest until day two using both non-targeted and targeted methods. A total of 859 metabolites and 411 lipids were identified by non-targeted methods, and 465 metabolites were quantitatively identified by targeted methods. This extensive data set provides a valuable resource for understanding systemic metabolic changes caused by race walking and helps researchers identify biomarkers of exercise performance.
Institute
The First Affiliated Hospital of Dalian Medical University
Last NameZhang
First Nameyunshu
AddressNo.222 Zhongshan Road, Xigang District, Dalian City, Liaoning Province
Emailzys1986062186@163.com
Phone0411-83635863
Submit Date2024-07-07
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-08-09
Release Version1
yunshu Zhang yunshu Zhang
https://dx.doi.org/10.21228/M8C802
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002083
Project DOI:doi: 10.21228/M8C802
Project Title:An integrated LC-MS analysis of the biometric characteristics of different time cohorts of race walkers
Project Summary:To obtain metabolic changes during endurance exercise, we conducted a metabolomics study in which 19 race walkers were recruited to collect blood at four time points: before, during, after, and after rest until day two using both non-targeted and targeted methods. A total of 859 metabolites and 411 lipids were identified by non-targeted methods, and 465 metabolites were quantitatively identified by targeted methods. This extensive data set provides a valuable resource for understanding systemic metabolic changes caused by race walking and helps researchers identify biomarkers of exercise performance.
Institute:The First Affiliated Hospital of Dalian Medical University
Last Name:Zhang
First Name:Yunshu
Address:No.222 Zhongshan Road, Xigang District, Dalian City, Liaoning Province, Dalian, Xigang District, Dalian City, Liaoning Province, 116000, China
Email:zys196062186@163.com
Phone:0411-93635863

Subject:

Subject ID:SU003469
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Collection_time
SA36520495_4blood rec22
SA36520589_4blood rec22
SA36520687_4blood rec22
SA36520790_4blood rec22
SA36520891_4blood rec22
SA36520986_4blood rec22
SA36521092_4blood rec22
SA36521185_4blood rec22
SA36521293_4blood rec22
SA36521394_4blood rec22
SA36521488_4blood rec22
SA36521584_4blood rec22
SA36521681_4blood rec22
SA36521798_4blood rec22
SA36521883_4blood rec22
SA36521996_4blood rec22
SA36522099_4blood rec22
SA36522179_4blood rec22
SA36522297_4blood rec22
SA36522399_3blood rec3
SA36522491_3blood rec3
SA36522596_3blood rec3
SA36522698_3blood rec3
SA36522792_3blood rec3
SA36522895_3blood rec3
SA36522993_3blood rec3
SA36523097_3blood rec3
SA36523194_3blood rec3
SA36523289_3blood rec3
SA36523390_3blood rec3
SA36523488_3blood rec3
SA36523584_3blood rec3
SA36523679_3blood rec3
SA36523781_3blood rec3
SA36523886_3blood rec3
SA36523987_3blood rec3
SA36524085_3blood rec3
SA36524183_3blood rec3
SA36524289_1blood rest
SA36524385_1blood rest
SA36524495_1blood rest
SA36524597_1blood rest
SA36524696_1blood rest
SA36524784_1blood rest
SA36524883_1blood rest
SA36524998_1blood rest
SA36525099_1blood rest
SA36525181_1blood rest
SA36525294_1blood rest
SA36525379_1blood rest
SA36525486_1blood rest
SA36525592_1blood rest
SA36525688_1blood rest
SA36525790_1blood rest
SA36525887_1blood rest
SA36525991_1blood rest
SA36526093_1blood rest
SA36526195_2blood stat
SA36526290_2blood stat
SA36526389_2blood stat
SA36526488_2blood stat
SA36526599_2blood stat
SA36526694_2blood stat
SA36526781_2blood stat
SA36526898_2blood stat
SA36526979_2blood stat
SA36527092_2blood stat
SA36527183_2blood stat
SA36527297_2blood stat
SA36527393_2blood stat
SA36527491_2blood stat
SA36527587_2blood stat
SA36527696_2blood stat
SA36527786_2blood stat
SA36527884_2blood stat
SA36527985_2blood stat
Showing results 1 to 76 of 76

Collection:

Collection ID:CO003462
Collection Summary:Sera were collected from 20 male student-athletes with the informed consent of every participants, and the study was approved by the ethics committee of Shenyang sport university (No.2022-6-30-01). Samples were collected at four different time points. The first point was fast samples collected before the race walk at 8:20 am (name as REST). The second point was collected at immediately after the race walk exercise at 10:30 am (name as STAT). The third point was collected at the 3-hour recovery period after the exercise at 13: 30 pm (named as REC3). The fourth point was fast samples collected at 22 h post-exercise on day 2 at 8:20 am (named as REC22). Blood were collected in plastic Vacutainer blood tubes, allowed to clot for 40 minutes, and then centrifuged at 13000 g for 10 minutes. The sera were frozen in dry ice immediately after collection and then stored at − 80°C untill analysis. One athlete quite during the study (No.80) and the data of heart rates in each timepoint is shown in Table 1.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR003478
Treatment Summary:There is no treatment involved in this study

Sample Preparation:

Sampleprep ID:SP003476
Sampleprep Summary:The aliquots were thawed at 4 ° C for 60 minutes and then gently blown with a pipetting gun to allow uniform distribution of the sample. Then, 120μL of serum was transferred to a 2 mL EP tube (Aixgen USA), and 480 μL of methanol was added. The samples were vortexed for 5 min then allowed to stand for 10 min at room temperature and centrifuged at 13000 g at 4 °C (Thermo Scientific, USA) for 10 minutes. The upper extracts were transferred to a 1.5ml tube and lyophilized for 3 hours in a vacuum freeze dryer (Labconco Corporation, USA) and then stored at 4 °C until injection. Prior to analysis, the extracts were redissolved in 500 μl acetonitril-water (1:3,v/v). Quality control (QC) samples were prepared by mixing equal amounts of samples.

Combined analysis:

Analysis ID AN005483 AN005484
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um) Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH004166
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um)
Column Temperature:50
Flow Gradient:2% B to 98% B in 10 min
Flow Rate:0.4 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH004167
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:50
Flow Gradient:2% B to 100% B in 10 min.
Flow Rate:0.4 mL/min
Solvent A:100% water
Solvent B:5% water/95% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS005209
Analysis ID:AN005483
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:POSITIVE
  
MS ID:MS005210
Analysis ID:AN005484
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:-
Ion Mode:NEGATIVE
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