Summary of Study ST003348
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002083. The data can be accessed directly via it's Project DOI: 10.21228/M8C802 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003348 |
Study Title | An integrated LC-MS analysis of the biometric characteristics of different time cohorts of race walkers - untargeted |
Study Summary | To obtain metabolic changes during endurance exercise, we conducted a metabolomics study in which 19 race walkers were recruited to collect blood at four time points: before, during, after, and after rest until day two using both non-targeted and targeted methods. A total of 859 metabolites and 411 lipids were identified by non-targeted methods, and 465 metabolites were quantitatively identified by targeted methods. This extensive data set provides a valuable resource for understanding systemic metabolic changes caused by race walking and helps researchers identify biomarkers of exercise performance. |
Institute | The First Affiliated Hospital of Dalian Medical University |
Last Name | Zhang |
First Name | yunshu |
Address | No.222 Zhongshan Road, Xigang District, Dalian City, Liaoning Province |
zys1986062186@163.com | |
Phone | 0411-83635863 |
Submit Date | 2024-07-07 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-08-09 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR002083 |
Project DOI: | doi: 10.21228/M8C802 |
Project Title: | An integrated LC-MS analysis of the biometric characteristics of different time cohorts of race walkers |
Project Summary: | This study investigates the metabolic changes induced by endurance exercise, specifically race walking, in a cohort of 19 athletes. Blood samples were collected at four time points: pre-exercise (REST), immediately post-exercise (STAT), 3 hours into recovery (REC3), and 22 hours post-exercise (REC22). A total of 859 metabolites were identified through the untargeted method, and 465 metabolites and 411 lipids were identified through the targeted methods. Rigorous quality control measures were implemented throughout the study to ensure data reliability. The comprehensive dataset, which is publicly available on the Metabolomics Workbench website, offers valuable insights into the systemic metabolic shifts triggered by endurance exercise. This resource may prove instrumental in uncovering biomarkers associated with athletic performance, providing a foundation for future research in exercise physiology and metabolic health. |
Institute: | The First Affiliated Hospital of Dalian Medical University |
Last Name: | Zhang |
First Name: | Yunshu |
Address: | No.222 Zhongshan Road, Xigang District, Dalian City, Liaoning Province, Dalian, Xigang District, Dalian City, Liaoning Province, 116000, China |
Email: | zys196062186@163.com |
Phone: | 0411-93635863 |
Subject:
Subject ID: | SU003469 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Sample source | Collection_time |
---|---|---|---|
SA365204 | 95_4 | blood | rec22 |
SA365205 | 89_4 | blood | rec22 |
SA365206 | 87_4 | blood | rec22 |
SA365207 | 90_4 | blood | rec22 |
SA365208 | 91_4 | blood | rec22 |
SA365209 | 86_4 | blood | rec22 |
SA365210 | 92_4 | blood | rec22 |
SA365211 | 85_4 | blood | rec22 |
SA365212 | 93_4 | blood | rec22 |
SA365213 | 94_4 | blood | rec22 |
SA365214 | 88_4 | blood | rec22 |
SA365215 | 84_4 | blood | rec22 |
SA365216 | 81_4 | blood | rec22 |
SA365217 | 98_4 | blood | rec22 |
SA365218 | 83_4 | blood | rec22 |
SA365219 | 96_4 | blood | rec22 |
SA365220 | 99_4 | blood | rec22 |
SA365221 | 79_4 | blood | rec22 |
SA365222 | 97_4 | blood | rec22 |
SA365223 | 99_3 | blood | rec3 |
SA365224 | 91_3 | blood | rec3 |
SA365225 | 96_3 | blood | rec3 |
SA365226 | 98_3 | blood | rec3 |
SA365227 | 92_3 | blood | rec3 |
SA365228 | 95_3 | blood | rec3 |
SA365229 | 93_3 | blood | rec3 |
SA365230 | 97_3 | blood | rec3 |
SA365231 | 94_3 | blood | rec3 |
SA365232 | 89_3 | blood | rec3 |
SA365233 | 90_3 | blood | rec3 |
SA365234 | 88_3 | blood | rec3 |
SA365235 | 84_3 | blood | rec3 |
SA365236 | 79_3 | blood | rec3 |
SA365237 | 81_3 | blood | rec3 |
SA365238 | 86_3 | blood | rec3 |
SA365239 | 87_3 | blood | rec3 |
SA365240 | 85_3 | blood | rec3 |
SA365241 | 83_3 | blood | rec3 |
SA365242 | 89_1 | blood | rest |
SA365243 | 85_1 | blood | rest |
SA365244 | 95_1 | blood | rest |
SA365245 | 97_1 | blood | rest |
SA365246 | 96_1 | blood | rest |
SA365247 | 84_1 | blood | rest |
SA365248 | 83_1 | blood | rest |
SA365249 | 98_1 | blood | rest |
SA365250 | 99_1 | blood | rest |
SA365251 | 81_1 | blood | rest |
SA365252 | 94_1 | blood | rest |
SA365253 | 79_1 | blood | rest |
SA365254 | 86_1 | blood | rest |
SA365255 | 92_1 | blood | rest |
SA365256 | 88_1 | blood | rest |
SA365257 | 90_1 | blood | rest |
SA365258 | 87_1 | blood | rest |
SA365259 | 91_1 | blood | rest |
SA365260 | 93_1 | blood | rest |
SA365261 | 95_2 | blood | stat |
SA365262 | 90_2 | blood | stat |
SA365263 | 89_2 | blood | stat |
SA365264 | 88_2 | blood | stat |
SA365265 | 99_2 | blood | stat |
SA365266 | 94_2 | blood | stat |
SA365267 | 81_2 | blood | stat |
SA365268 | 98_2 | blood | stat |
SA365269 | 79_2 | blood | stat |
SA365270 | 92_2 | blood | stat |
SA365271 | 83_2 | blood | stat |
SA365272 | 97_2 | blood | stat |
SA365273 | 93_2 | blood | stat |
SA365274 | 91_2 | blood | stat |
SA365275 | 87_2 | blood | stat |
SA365276 | 96_2 | blood | stat |
SA365277 | 86_2 | blood | stat |
SA365278 | 84_2 | blood | stat |
SA365279 | 85_2 | blood | stat |
Showing results 1 to 76 of 76 |
Collection:
Collection ID: | CO003462 |
Collection Summary: | Sera were collected from 20 male student-athletes with the informed consent of every participants, and the study was approved by the ethics committee of Shenyang sport university (No.2022-6-30-01). Samples were collected at four different time points. The first point was fast samples collected before the race walk at 8:20 am (name as REST). The second point was collected at immediately after the race walk exercise at 10:30 am (name as STAT). The third point was collected at the 3-hour recovery period after the exercise at 13: 30 pm (named as REC3). The fourth point was fast samples collected at 22 h post-exercise on day 2 at 8:20 am (named as REC22). Blood were collected in plastic Vacutainer blood tubes, allowed to clot for 40 minutes, and then centrifuged at 13000 g for 10 minutes. The sera were frozen in dry ice immediately after collection and then stored at − 80°C untill analysis. One athlete quite during the study (No.80) and the data of heart rates in each timepoint is shown in Table 1. |
Sample Type: | Blood (serum) |
Treatment:
Treatment ID: | TR003478 |
Treatment Summary: | There is no treatment involved in this study |
Sample Preparation:
Sampleprep ID: | SP003476 |
Sampleprep Summary: | The aliquots were thawed at 4 ° C for 60 minutes and then gently blown with a pipetting gun to allow uniform distribution of the sample. Then, 120μL of serum was transferred to a 2 mL EP tube (Aixgen USA), and 480 μL of methanol was added. The samples were vortexed for 5 min then allowed to stand for 10 min at room temperature and centrifuged at 13000 g at 4 °C (Thermo Scientific, USA) for 10 minutes. The upper extracts were transferred to a 1.5ml tube and lyophilized for 3 hours in a vacuum freeze dryer (Labconco Corporation, USA) and then stored at 4 °C until injection. Prior to analysis, the extracts were redissolved in 500 μl acetonitril-water (1:3,v/v). Quality control (QC) samples were prepared by mixing equal amounts of samples. |
Combined analysis:
Analysis ID | AN005483 | AN005484 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um) | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH004166 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um) |
Column Temperature: | 50 |
Flow Gradient: | 2% B to 98% B in 10 min |
Flow Rate: | 0.4 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH004167 |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
Column Temperature: | 50 |
Flow Gradient: | 2% B to 100% B in 10 min. |
Flow Rate: | 0.4 mL/min |
Solvent A: | 100% water |
Solvent B: | 5% water/95% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS005209 |
Analysis ID: | AN005483 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | POSITIVE |
MS ID: | MS005210 |
Analysis ID: | AN005484 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | - |
Ion Mode: | NEGATIVE |