Summary of Study ST003349

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002083. The data can be accessed directly via it's Project DOI: 10.21228/M8C802 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003349
Study TitleAn integrated LC-MS analysis of the biometric characteristics of different time cohorts of race walkers - targeted
Study SummaryTo obtain the metabolic alterations during endurance exercise, we performed a metabolomics study that enrolled 19 race-walking athletes using untargeted and targeted approaches. In total, 859 metabolites and 411 lipids were identified in the untargeted methods, and 465 metabolites were quantified from targeted methods. This extensive dataset offers a valuable resource for understanding the systemic metabolic changes induced by race walking and assists researchers in identifying biomarkers of athletic performance.
Institute
The First Affiliated Hospital of Dalian Medical University
Last NameZhang
First Nameyunshu
AddressNo.222 Zhongshan Road, Xigang District, Dalian City, Liaoning Province
Emailzys1986062186@163.com
Phone0411-83635863
Submit Date2024-07-10
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2024-08-09
Release Version1
yunshu Zhang yunshu Zhang
https://dx.doi.org/10.21228/M8C802
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002083
Project DOI:doi: 10.21228/M8C802
Project Title:An integrated LC-MS analysis of the biometric characteristics of different time cohorts of race walkers
Project Summary:To obtain metabolic changes during endurance exercise, we conducted a metabolomics study in which 19 race walkers were recruited to collect blood at four time points: before, during, after, and after rest until day two using both non-targeted and targeted methods. A total of 859 metabolites and 411 lipids were identified by non-targeted methods, and 465 metabolites were quantitatively identified by targeted methods. This extensive data set provides a valuable resource for understanding systemic metabolic changes caused by race walking and helps researchers identify biomarkers of exercise performance.
Institute:The First Affiliated Hospital of Dalian Medical University
Last Name:Zhang
First Name:Yunshu
Address:No.222 Zhongshan Road, Xigang District, Dalian City, Liaoning Province, Dalian, Xigang District, Dalian City, Liaoning Province, 116000, China
Email:zys196062186@163.com
Phone:0411-93635863

Subject:

Subject ID:SU003470
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source collection time
SA36528095_4blood rec22
SA36528189_4blood rec22
SA36528287_4blood rec22
SA36528390_4blood rec22
SA36528491_4blood rec22
SA36528586_4blood rec22
SA36528692_4blood rec22
SA36528785_4blood rec22
SA36528893_4blood rec22
SA36528994_4blood rec22
SA36529088_4blood rec22
SA36529184_4blood rec22
SA36529281_4blood rec22
SA36529398_4blood rec22
SA36529483_4blood rec22
SA36529596_4blood rec22
SA36529699_4blood rec22
SA36529779_4blood rec22
SA36529897_4blood rec22
SA36529999_3blood rec3
SA36530091_3blood rec3
SA36530196_3blood rec3
SA36530298_3blood rec3
SA36530392_3blood rec3
SA36530495_3blood rec3
SA36530593_3blood rec3
SA36530697_3blood rec3
SA36530794_3blood rec3
SA36530889_3blood rec3
SA36530990_3blood rec3
SA36531088_3blood rec3
SA36531184_3blood rec3
SA36531279_3blood rec3
SA36531381_3blood rec3
SA36531486_3blood rec3
SA36531587_3blood rec3
SA36531685_3blood rec3
SA36531783_3blood rec3
SA36531889_1blood rest
SA36531985_1blood rest
SA36532095_1blood rest
SA36532197_1blood rest
SA36532296_1blood rest
SA36532384_1blood rest
SA36532483_1blood rest
SA36532598_1blood rest
SA36532699_1blood rest
SA36532781_1blood rest
SA36532894_1blood rest
SA36532979_1blood rest
SA36533086_1blood rest
SA36533192_1blood rest
SA36533288_1blood rest
SA36533390_1blood rest
SA36533487_1blood rest
SA36533591_1blood rest
SA36533693_1blood rest
SA36533795_2blood stat
SA36533890_2blood stat
SA36533989_2blood stat
SA36534088_2blood stat
SA36534199_2blood stat
SA36534294_2blood stat
SA36534381_2blood stat
SA36534498_2blood stat
SA36534579_2blood stat
SA36534692_2blood stat
SA36534783_2blood stat
SA36534897_2blood stat
SA36534993_2blood stat
SA36535091_2blood stat
SA36535187_2blood stat
SA36535296_2blood stat
SA36535386_2blood stat
SA36535484_2blood stat
SA36535585_2blood stat
Showing results 1 to 76 of 76

Collection:

Collection ID:CO003463
Collection Summary:Sera were collected from 20 male student-athletes with the informed consent of every participants, and the study was approved by the ethics committee of Shenyang sport university (No.2022-6-30-01). Samples were collected at four different time points. The first point was fast samples collected before the race walk at 8:20 am (name as REST). The second point was collected at immediately after the race walk exercise at 10:30 am (name as STAT). The third point was collected at the 3-hour recovery period after the exercise at 13: 30 pm (named as REC3). The fourth point was fast samples collected at 22 h post-exercise on day 2 at 8:20 am (named as REC22). Blood were collected in plastic Vacutainer blood tubes, allowed to clot for 40 minutes, and then centrifuged at 13000 g for 10 minutes. The sera were frozen in dry ice immediately after collection and then stored at − 80°C untill analysis.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR003479
Treatment Summary:There is no treatment.

Sample Preparation:

Sampleprep ID:SP003477
Sampleprep Summary:The aliquots were thawed at 4 °C for 60 minutes and then gently blown with a pipetting gun to allow uniform distribution of the sample. Then, 120μL of serum was transferred to a 2 mL EP tube (Aixgen USA), and 480 μL of methanol was added. The samples were vortexed for 5 min then allowed to stand for 10 min at room temperature and centrifuged at 13000 g at 4 °C (Thermo Scientific, USA) for 10 minutes. The upper extracts were transferred to a 1.5ml tube and lyophilized for 3 hours in a vacuum freeze dryer (Labconco Corporation, USA) and then stored at 4 °C until injection. Prior to analysis, the extracts were redissolved in 500 μl acetonitril-water (1:3,v/v). Quality control (QC) samples were prepared by mixing equal amounts of samples.

Combined analysis:

Analysis ID AN005485 AN005486 AN005487 AN005488 AN005489 AN005490
Analysis type MS MS MS MS MS MS
Chromatography type HILIC Reversed phase Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Shimadzu Nexera X2 Shimadzu Nexera X2 Shimadzu Nexera X2 Shimadzu Nexera X2 Shimadzu Nexera X2 Shimadzu Nexera X2
Column Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) Thermo Hypersil GOLD aQ (100 x 2.1mm,1.9um) Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um) ACE Excel 2 C18-PFP (100 x 2.1mm,2um) ACE Excel 2 C18-PFP (100 x 2.1mm,2um) Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um)
MS Type ESI ESI ESI ESI ESI ESI
MS instrument type Triple quadrupole Triple quadrupole Triple quadrupole Triple quadrupole Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap
Ion Mode UNSPECIFIED UNSPECIFIED UNSPECIFIED UNSPECIFIED UNSPECIFIED UNSPECIFIED
Units ng/mL ng/mL ng/mL ng/mL μM ng/mL

Chromatography:

Chromatography ID:CH004168
Chromatography Summary:Solvent A: 90% aqueous acetonitrile Solvent B: 50% aqueous acetonitrile
Instrument Name:Shimadzu Nexera X2
Column Name:Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um)
Column Temperature:40
Flow Gradient:0% B to 70% B, the elution time was 15 min
Flow Rate:0.3 mL/min
Solvent A:90% acetonitrile/10% water
Solvent B:50% acetonitrile/50% water
Chromatography Type:HILIC
  
Chromatography ID:CH004169
Instrument Name:Shimadzu Nexera X2
Column Name:Thermo Hypersil GOLD aQ (100 x 2.1mm,1.9um)
Column Temperature:40
Flow Gradient:0.5-12 min 0-17%B, 12-15.5 min 17-30%B, 15.5-16.5 min 55%B, 16.5-24.5 min 55-95%B, 24.5-27.0 min 95%B. 27.0-27.1 min 95-17%B
Flow Rate:0.4 mL/min
Solvent A:100% water
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase
  
Chromatography ID:CH004170
Chromatography Summary:RP chemical derivatization method
Instrument Name:Shimadzu Nexera X2
Column Name:Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Gradient:0-1min,15%B; 1-10min,15%B, 10-16min,15-40%B;16-23min,40-95%B,23-26min 95%B,26-27min 95-15%
Flow Rate:0.3 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase
  
Chromatography ID:CH004171
Instrument Name:Shimadzu Nexera X2
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm,2um)
Column Temperature:40
Flow Gradient:0-1min 5%B; 1-9min 5%-75%B; 9-9.5min 75%-95%B; 9.5-12min 95%B; 12-12.5min 95%B-5%B
Flow Rate:0.3 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase
  
Chromatography ID:CH004172
Instrument Name:Shimadzu Nexera X2
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm,2um)
Column Temperature:40
Flow Gradient:2% to 98% B in 12 min; 12-13.5min 98%B; 13.5-13.6min 98%-2%B
Flow Rate:0.4 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH004173
Instrument Name:Shimadzu Nexera X2
Column Name:Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Gradient:0-2.0 min at 20% B, 2.0-5.0 min 20 to 70%B, 5.0-17.0 min from 70 to 95%B, 17.0-17.5 min at 95%B, 17.6 min at 20%B with 2.4 min for re-equilibration.
Flow Rate:0.2 mL/min
Solvent A:60% acetonitrile/40% water; 5mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 5mM ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS005211
Analysis ID:AN005485
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Polarity switching for MS was used.
Ion Mode:UNSPECIFIED
  
MS ID:MS005212
Analysis ID:AN005486
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Polarity switching for MS was used.
Ion Mode:UNSPECIFIED
  
MS ID:MS005213
Analysis ID:AN005487
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Polarity switching for MS was used.
Ion Mode:UNSPECIFIED
  
MS ID:MS005214
Analysis ID:AN005488
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Polarity switching for MS was used.
Ion Mode:UNSPECIFIED
  
MS ID:MS005215
Analysis ID:AN005489
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Polarity switching for MS was used.
Ion Mode:UNSPECIFIED
  
MS ID:MS005216
Analysis ID:AN005490
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:Polarity switching for MS was used.
Ion Mode:UNSPECIFIED
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