Summary of Study ST003586

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002216. The data can be accessed directly via it's Project DOI: 10.21228/M85Z4M This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST003586
Study Title	Metabolomics of the murine liver
Study Summary	To examine whether a molecule associated with metabolic liver zonation has an effect on systemic metabolism, we first administer adenoviruses expressing Rspo3 or LacZ (control) into the murine liver. Then, by performing metabolome analyses, we compare the results of Rspo3-mice with those of LacZ-mice.
Institute	Teikyo University
Last Name	Uno
First Name	Kenji
Address	2-11-1 Kaga, Itabashi-ku, Tokyo
Email	unok@med.teikyo-u.ac.jp
Phone	81339641211
Submit Date	2024-11-19
Analysis Type Detail	LC-MS
Release Date	2024-12-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR002216
Project DOI:	doi: 10.21228/M85Z4M
Project Title:	Metabolomics of the liver after administration of adenovirus associated with metabolic liver zonation
Project Summary:	Metabolic zonation in the liver is broadly defined as spatiotemporal variability in the intra-hepatic distribution of nutrients. R-spondin3 (Rspo3) regulates metabolic features around hepatic central veins. Herein, we demonstrate that altered hepatic metabolic zonation due to Rspo3 contributes to maintaining systemic glucose metabolism and body composition via inter-organ communication mechanism. Hepatic Rspo3 induction in obesity improves obesity-associated features by restoring insulin sensitivity, reversing adipose tissue enlargement and reversing overstimulated adaptive thermogenesis. These remote effects partly consist of neuronal communication via the afferent vagus from the liver. In contrast, hepatic Rspo3 suppression exacerbates diabetes due to insulin resistance and develops fatty liver.
Institute:	Teikyo University
Last Name:	Uno
First Name:	Kenji
Address:	2-11-1 Kaga, Itabashi-ku, Tokyo
Email:	unok@med.teikyo-u.ac.jp
Phone:	81339641211

Subject:

Subject ID:	SU003715
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample source	Group
SA391395	ob-LacZ-1	liver	ob-LacZ
SA391396	ob-LacZ-2	liver	ob-LacZ
SA391397	ob-LacZ-3	liver	ob-LacZ
SA391398	ob-LacZ-4	liver	ob-LacZ
SA391399	ob-Rspo3-1	liver	ob-Rspo3
SA391400	ob-Rspo3-2	liver	ob-Rspo3
SA391401	ob-Rspo3-3	liver	ob-Rspo3
SA391402	ob-Rspo3-4	liver	ob-Rspo3

Showing results 1 to 8 of 8

Showing results 1 to 8 of 8

Collection:

Collection ID:	CO003708
Collection Summary:	Liver tissues were rapidly removed on day 7 after adenovirus administration, frozen on liquid nitrogen and stored at –80°C until further processing. Then, liver samples were weighed at approximately 40 mg and subjected to the experiment of metabolomics.
Sample Type:	Liver

Treatment:

Treatment ID:	TR003724
Treatment Summary:	Recombinant adenoviruses expressing LacZ or Rspo3 were administered into the liver of standard diet-fed ob/ob mice through tail vein injection. Liver tissues were taken on day 7 after adenovirus administration, subjected to the experiment of metabolomics.

Sample Preparation:

Sampleprep ID:	SP003722
Sampleprep Summary:	Approximately 40 mg of frozen liver tissues were placed in a homogenization tube, along with zirconia beads (5mmφ and 3mmφ). These tissues with internal standards were homogenized with ice-cold acetonitrile:water (50:50) and centrifuged at 2,300 × g for 5 min at 4 °C. The supernatant was filtered through a 5-kDa cut-off filter (Ultrafree-MC-PLHCC, Human Metabolome Technologies, Japan) at 9,100 × g for 180 min at 4 °C. The filtrate was evaporated to dryness under vacuum and reconstituted in 50 µL of Milli-Q water for metabolome analysis at HMT.

Sampleprep ID:

SP003722

Sampleprep Summary:

Approximately 40 mg of frozen liver tissues were placed in a homogenization tube, along with zirconia beads (5mmφ and 3mmφ). These tissues with internal standards were homogenized with ice-cold acetonitrile:water (50:50) and centrifuged at 2,300 × g for 5 min at 4 °C. The supernatant was filtered through a 5-kDa cut-off filter (Ultrafree-MC-PLHCC, Human Metabolome Technologies, Japan) at 9,100 × g for 180 min at 4 °C. The filtrate was evaporated to dryness under vacuum and reconstituted in 50 µL of Milli-Q water for metabolome analysis at HMT.

Combined analysis:

Analysis ID	AN005889	AN005890
Analysis type	MS	MS
Chromatography type	CE	CE
Chromatography system	Agilent 7100 CE	Agilent 7100 CE
Column	Fused silica capillary i.d. 50 um x 80 cm	Fused silica capillary i.d. 50 um x 80 cm
MS Type	ESI	ESI
MS instrument type	TOF	TOF
MS instrument name	Agilent 6230 TOF	Agilent 6230 TOF
Ion Mode	POSITIVE	NEGATIVE
Units	arbitrary unit	arbitrary unit

Chromatography:

Chromatography ID:	CH004472
Instrument Name:	Agilent 7100 CE
Column Name:	Fused silica capillary i.d. 50 um x 80 cm
Column Temperature:	N/A
Flow Gradient:	N/A
Flow Rate:	N/A
Solvent A:	Cation Buffer Solution (p/n : H3301-1001)
Solvent B:	N/A
Chromatography Type:	CE

Chromatography ID:	CH004473
Instrument Name:	Agilent 7100 CE
Column Name:	Fused silica capillary i.d. 50 um x 80 cm
Column Temperature:	N/A
Flow Gradient:	N/A
Flow Rate:	N/A
Solvent A:	Anion Buffer Solution (p/n : I3302-1023)
Solvent B:	N/A
Chromatography Type:	CE

MS:

MS ID:	MS005607
Analysis ID:	AN005889
Instrument Name:	Agilent 6230 TOF
Instrument Type:	TOF
MS Type:	ESI
MS Comments:	The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using automatic integration software MasterHands (ver. 2.19.0.2 Keio University, Japan). Signal peaks corresponding to isotopomers, adduct ions, and other product ions of known metabolites were excluded, and remaining peaks were annotated with putative metabolites from the HMT metabolite database based on their migration times (MTs) and m/z values. The tolerance range for the peak annotation was configured at ±0.5 min for MT and ±10 ppm for m/z. In addition, peak areas were normalized against those of the internal standards and then the resultant relative area values were further normalized by sample amount.
Ion Mode:	POSITIVE

MS ID:	MS005608
Analysis ID:	AN005890
Instrument Name:	Agilent 6230 TOF
Instrument Type:	TOF
MS Type:	ESI
MS Comments:	The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using automatic integration software MasterHands (ver. 2.19.0.2 Keio University, Japan). Signal peaks corresponding to isotopomers, adduct ions, and other product ions of known metabolites were excluded, and remaining peaks were annotated with putative metabolites from the HMT metabolite database based on their migration times (MTs) and m/z values. The tolerance range for the peak annotation was configured at ±0.5 min for MT and ±10 ppm for m/z. In addition, peak areas were normalized against those of the internal standards and then the resultant relative area values were further normalized by sample amount.
Ion Mode:	NEGATIVE