Summary of Study ST003600

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002228. The data can be accessed directly via it's Project DOI: 10.21228/M8MZ51 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003600
Study Title	Development of Food Material Source Technology for Future Alternative Meats (Including Cultured Meat)
Study Summary	The growing demand for sustainable food sources has accelerated the development of cultured meat as an alternative to traditional meat products. This study aims to predict the safety and nutritional equivalence of cultured meat compared to conventional meat using a comprehensive metabolomics approach. In this study, we conducted a comparative metabolomic analysis of conventional chicken meat, muscle satellite cells, and differentiated cells. Our findings reveal that while the overall metabolic profiles of cultured and original meats are comparable, significant differences are observed in specific metabolites. Notably, metabolites associated with nutrient metabolism and synthesis display substantial variations among the samples. These differences suggest that the nutritional content of cultured meat may differ from that of traditional meat, potentially affecting its dietary value. Despite these differences in metabolic profiles, our analysis indicates that there is no significant impact on the safety of cultured meat itself. The safety of cultured meat remains within acceptable limits, and no adverse health risks were identified in the context of this study. However, the observed variations in nutrient-related metabolites highlight the need for further investigation to fully understand their implications. Our study contributes to the ongoing evaluation of cultured meat as a viable and safe alternative in the pursuit of sustainable food sources.
Institute	Sangmyung University
Last Name	Ihyeon
First Name	Cho
Address	Jongno-gu, Seoul, 서울, 03057, South Korea
Email	mukuro259@gmail.com
Phone	01021146997
Submit Date	2024-11-19
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2024-12-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR002228
Project DOI:	doi: 10.21228/M8MZ51
Project Title:	Metabolomic Insights of Cultured Meat
Project Summary:	The growing demand for sustainable food sources has accelerated the development of cultured meat as an alternative to traditional meat products. This study aims to predict the safety and nutritional equivalence of cultured meat compared to conventional meat using a comprehensive metabolomics approach. In this study, we conducted a comparative metabolomic analysis of conventional chicken meat, muscle satellite cells, and differentiated cells. Our findings reveal that while the overall metabolic profiles of cultured and original meats are comparable, significant differences are observed in specific metabolites. Notably, metabolites associated with nutrient metabolism and synthesis display substantial variations among the samples. These differences suggest that the nutritional content of cultured meat may differ from that of traditional meat, potentially affecting its dietary value. Despite these differences in metabolic profiles, our analysis indicates that there is no significant impact on the safety of cultured meat itself. The safety of cultured meat remains within acceptable limits, and no adverse health risks were identified in the context of this study. However, the observed variations in nutrient-related metabolites highlight the need for further investigation to fully understand their implications. Our study contributes to the ongoing evaluation of cultured meat as a viable and safe alternative in the pursuit of sustainable food sources.
Institute:	Sangmyung University
Department:	Department of Foodservice Management and Nutrition
Last Name:	Ihyeon
First Name:	Cho
Address:	Jongno-gu, Seoul, 서울, 03057, South Korea
Email:	mukuro259@gmail.com
Phone:	01021146997

Subject:

Subject ID:	SU003729
Subject Type:	Other organism
Subject Species:	Gallus gallus
Taxonomy ID:	9031
Species Group:	Birds

Factors:

Subject type: Other organism; Subject species: Gallus gallus (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample source	Type
SA392480	CON_2_pos	chicken meat	traditional meat
SA392481	CON_3_neg	chicken meat	traditional meat
SA392482	CON_2_neg	chicken meat	traditional meat
SA392483	CON_1_pos	chicken meat	traditional meat
SA392484	CON_3_pos	chicken meat	traditional meat
SA392485	CON_1_neg	chicken meat	traditional meat
SA392486	CAC_1_pos	muscle cell	cultured meat
SA392487	CAC_2_pos	muscle cell	cultured meat
SA392488	CAC_3_pos	muscle cell	cultured meat
SA392489	CAC_1_neg	muscle cell	cultured meat
SA392490	CAC_2_neg	muscle cell	cultured meat
SA392491	CAC_3_neg	muscle cell	cultured meat
SA392492	CIC_3_pos	muscle satellite cell	cultured meat
SA392493	CIC_2_pos	muscle satellite cell	cultured meat
SA392494	CIC_1_pos	muscle satellite cell	cultured meat
SA392495	CIC_1_neg	muscle satellite cell	cultured meat
SA392496	CIC_2_neg	muscle satellite cell	cultured meat
SA392497	CIC_3_neg	muscle satellite cell	cultured meat

Showing results 1 to 18 of 18

Showing results 1 to 18 of 18

Collection:

Collection ID:	CO003722
Collection Summary:	Cultured meat obtained from leading commercial producers (Neocrema, Seongnam, Korea). Original meat sourced from conventional farming (Harim, Iksan, Korea).
Sample Type:	Cultured cells

Treatment:

Treatment ID:	TR003738
Treatment Summary:	No treatment

Sample Preparation:

Sampleprep ID:	SP003736
Sampleprep Summary:	The samples were suspended in lysis buffer (50 mM ammonium bicarbonate), followed by sonication for 12 min at 15°C using Covaris S2 Focused-Ultrasonicator (Covaris, Woburn, MA, USA). The concentration of protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Metabolites were extracted from each 100 µg of protein sample by incubation with 4 volumes of cold methanol solution at -20°C for 2 hours. After centrifugation at 14,000×g for 10 min, the supernatant was transferred to a new 1.5 mL tube and completely dried using a speed-vac centrifugal vacuum concentrator(Vision Scientific, Daejeon, Korea). Dried metabolite contents were reconstituted in 100 µL of 0.1% formic acid in water. Reconstituted samples were transferred to autosampler vials and then subjected to LC-MS/MS analysis.

Sampleprep ID:

SP003736

Sampleprep Summary:

The samples were suspended in lysis buffer (50 mM ammonium bicarbonate), followed by sonication for 12 min at 15°C using Covaris S2 Focused-Ultrasonicator (Covaris, Woburn, MA, USA). The concentration of protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Metabolites were extracted from each 100 µg of protein sample by incubation with 4 volumes of cold methanol solution at -20°C for 2 hours. After centrifugation at 14,000×g for 10 min, the supernatant was transferred to a new 1.5 mL tube and completely dried using a speed-vac centrifugal vacuum concentrator(Vision Scientific, Daejeon, Korea). Dried metabolite contents were reconstituted in 100 µL of 0.1% formic acid in water. Reconstituted samples were transferred to autosampler vials and then subjected to LC-MS/MS analysis.

Combined analysis:

Analysis ID	AN005915	AN005916
Analysis type	MS	MS
Chromatography type	Reversed phase	Reversed phase
Chromatography system	Thermo Vanquish	Thermo Vanquish
Column	Agilent Eclipse Plus C18 RRHD (50 × 2.1mm, 1.8um)	Agilent Eclipse Plus C18 RRHD (50 × 2.1mm, 1.8um)
MS Type	ESI	ESI
MS instrument type	Orbitrap	Orbitrap
MS instrument name	Thermo Q Exactive Orbitrap	Thermo Q Exactive Orbitrap
Ion Mode	POSITIVE	NEGATIVE
Units	peak area	peak area

Chromatography:

Chromatography ID:	CH004492
Chromatography Summary:	LC-MS/MS analysis for metabolomics was performed using a Q-Exactive Orbitrap hybrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) along with a Vanquish UHPLC system (Thermo Fisher Scientific, Waltham, MA, USA). A 5 μL sample solution was injected into an Eclipse Plus C18 RRHD column (50 × 2.1 mm; id. 1.8 μm; Agilent, CA, USA) at 40°C. The flow rate of the mobile phase was 0.2 mL/min. Analytes were eluted from the column under a gradient (solvent A, 0.1% formic acid in water; solvent B, 0.1% formic acid in 80% acetonitrile). The elution gradient was as follows: 2.5% B for 2 min, 2–12% B over 2–11 min, 12–28% B over 11–15 min; 28–60% B over 15–22 min; 96% B over 22–26 min; returned to 2.5% B for 5 min. Solvent A was run every sample as a blank solution.
Instrument Name:	Thermo Vanquish
Column Name:	Agilent Eclipse Plus C18 RRHD (50 × 2.1mm, 1.8um)
Column Temperature:	40°C
Flow Gradient:	2.5% B for 2 min, 2.0–12% B over 2–11 min, 12–28% B over 11–15 min; 28–60% B over 15–22 min; 96% B over 22–26 min; returned to 2.5% B for 5 min
Flow Rate:	0.2 mL/min
Solvent A:	100% water; 0.1% formic acid
Solvent B:	80% acetonitrile/20% water; 0.1% formic acid
Chromatography Type:	Reversed phase

MS:

MS ID:	MS005633
Analysis ID:	AN005915
Instrument Name:	Thermo Q Exactive Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Mass spectrometer parameters were as follows: Full‐MS scans, 100 to 1,000 (Positive mode) and 50 to 500 (Negative mode) m/z of scan range, 70,000 of resolution, 1 × 106 of AGC target, and maximum IT of 100 ms; For MS2 scans, the following parameters were used: 17,500 of resolution, 1 × 105 of AGC target, maximum IT was 200 ms, ±2 m/z of isolation width, and NCE for dd‐MS2 of 30; sheath gas flow rate was 19 (Positive mode) and 5 (Negative mode); aux gas flow rate was 1; spray voltage was 3.80 kV(Positive mode) and −3.40 kV(Negative mode); capillary temp was 320 °C; S-lens RF level was 60.0. Using Compound Discoverer 3.3™ (Thermo Fisher Scientific, Waltham, MA, USA), data analysis is conducted from LC-MS/MS raw files of each sample. Analysis is performed in the 'Untargeted Metabolomics with Statistics Detect Unknowns with ID using online Data Base and mzlogic' mode. Compound identification is carried out using mzCloud (ddMS2, https://www.mzcloud.org/) and ChemSpider (formula or exact mass, http:// www.chemspider.com), followed by ddMS2 data similarity searches for all compounds using mzCloud. According to the Metabolomics Standards Initiative (MSI) criteria, compounds are filtered at Level 2 (< 10 ppm, mzcloud score > 80) and Level 1 (ChemSpider < 5 ppm).12 Metabolites with CV values of area exceeding 30% in QC (Pooled samples) are excluded, and duplicate metabolites are removed based on average area intensity. Based on the qualitative and quantitative analysis results of identified metabolites, statistical analysis is performed using MetaboAnalyst 6.0 (http://www.metaboanalyst.ca).
Ion Mode:	POSITIVE

MS ID:	MS005634
Analysis ID:	AN005916
Instrument Name:	Thermo Q Exactive Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Mass spectrometer parameters were as follows: Full‐MS scans, 100 to 1,000 (Positive mode) and 50 to 500 (Negative mode) m/z of scan range, 70,000 of resolution, 1 × 106 of AGC target, and maximum IT of 100 ms; For MS2 scans, the following parameters were used: 17,500 of resolution, 1 × 105 of AGC target, maximum IT was 200 ms, ±2 m/z of isolation width, and NCE for dd‐MS2 of 30; sheath gas flow rate was 19 (Positive mode) and 5 (Negative mode); aux gas flow rate was 1; spray voltage was 3.80 kV(Positive mode) and −3.40 kV(Negative mode); capillary temp was 320 °C; S-lens RF level was 60.0. Using Compound Discoverer 3.3™ (Thermo Fisher Scientific, Waltham, MA, USA), data analysis is conducted from LC-MS/MS raw files of each sample. Analysis is performed in the 'Untargeted Metabolomics with Statistics Detect Unknowns with ID using online Data Base and mzlogic' mode. Compound identification is carried out using mzCloud (ddMS2, https://www.mzcloud.org/) and ChemSpider (formula or exact mass, http:// www.chemspider.com), followed by ddMS2 data similarity searches for all compounds using mzCloud. According to the Metabolomics Standards Initiative (MSI) criteria, compounds are filtered at Level 2 (< 10 ppm, mzcloud score > 80) and Level 1 (ChemSpider < 5 ppm).12 Metabolites with CV values of area exceeding 30% in QC (Pooled samples) are excluded, and duplicate metabolites are removed based on average area intensity. Based on the qualitative and quantitative analysis results of identified metabolites, statistical analysis is performed using MetaboAnalyst 6.0 (http://www.metaboanalyst.ca).
Ion Mode:	NEGATIVE