Summary of Study ST003612

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002232. The data can be accessed directly via it's Project DOI: 10.21228/M8424B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003612
Study Title	Candida auris planktonic and dispersed cells Chromatographic analysis using GC-MS
Study Summary	The emerging Candida auris (C. auris) has caused several outbreaks globally. While several studies explored the resistant biofilm formed by C. auris, little is known regarding the cells dispersed following biofilm formation. Herein, I investigated and characterized the cells dispersed from C. auris biofilms. Cells dispersed from biofilm developed in 96 well plate were isolated and counted. GC-MS analysis indicated that dispersed cells exhibit altered metabolic profile that enhance cells survivability under stress and nutrient limit condition. The presented study is the first to explore C. auris dispersed cells and indicated that they are not able to revert to the planktonic mode once released from the biofilm.
Institute	National Research Centre, Dokki, Egypt
Last Name	Fayed
First Name	Bahgat
Address	Dokki
Email	bm.ezzat@nrc.sci.eg
Phone	+201098292083
Submit Date	2024-11-23
Num Groups	two
Raw Data Available	Yes
Raw Data File Type(s)	wmf (Windows Metafile Format)
Analysis Type Detail	GC-MS
Release Date	2024-12-27
Release Version	1

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Project:

Project ID:	PR002232
Project DOI:	doi: 10.21228/M8424B
Project Title:	Candida auris planktonic and dispersed cells Chromatographic analysis using GC-MS
Project Summary:	The emerging Candida auris (C. auris) has caused several outbreaks globally. While several studies explored the resistant biofilm formed by C. auris, little is known regarding the cells dispersed following biofilm formation. Herein, I investigated and characterized the cells dispersed from C. auris biofilms. Cells dispersed from biofilm developed in 96 well plate were isolated and counted. GC-MS analysis indicated that dispersed cells exhibit altered metabolic profile that enhance cells survivability under stress and nutrient limit condition. The presented study is the first to explore C. auris dispersed cells and indicated that they are not able to revert to the planktonic mode once released from the biofilm.
Institute:	National Research Centre, Dokki, Egypt
Last Name:	Fayed
First Name:	Bahgat
Address:	Dokki, Giza, Cairo, 11433, Egypt
Email:	bm.ezzat@nrc.sci.eg
Phone:	+201098292083

Subject:

Subject ID:	SU003741
Subject Type:	Yeast
Subject Species:	Candida auris

Factors:

Subject type: Yeast; Subject species: Candida auris (Factor headings shown in green)

mb_sample_id	local_sample_id	Sample source	Geno type
SA392800	sample tested	Candida auris dispersed cells	Wild
SA392801	Control	Candida auris planktonic cells	Wild

Showing results 1 to 2 of 2

Showing results 1 to 2 of 2

Collection:

Collection ID:	CO003734
Collection Summary:	C. auris dispersed cell were allowed to grow in 250-mL Erlenmeyer flasks containing 50 mL of SD broth media for 3 days at 30°C. Following incubation, the supernatant was separated by centrifugation for 20 min at 4000 rpm. The resulting supernatant was extracted with 100 mL ethyl acetate.
Sample Type:	Candida auris

Treatment:

Treatment ID:	TR003750
Treatment Summary:	Isolation of cells dispersed from C. auris biofilm C. auris biofilms were developed by loading 1×106 cells/ml in RPMI-1640 supplemented with 2% glucose and then incubating for 24 h at 37°C in 96-well flat-bottomed microplates [17]. Following incubation, the supernatant containing floating cells was removed, the formed biofilm was washed 3 times with PBS, fresh media was added, and the biofilm was incubated for an additional 24 h under the same conditions. The next day, the cells dispersed from the biofilm in the upper supernatant were collected, counted by a hemocytometer and maintained in SD media or stored in 30% glycerol for further use.

Treatment ID:

TR003750

Treatment Summary:

Isolation of cells dispersed from C. auris biofilm C. auris biofilms were developed by loading 1×106 cells/ml in RPMI-1640 supplemented with 2% glucose and then incubating for 24 h at 37°C in 96-well flat-bottomed microplates [17]. Following incubation, the supernatant containing floating cells was removed, the formed biofilm was washed 3 times with PBS, fresh media was added, and the biofilm was incubated for an additional 24 h under the same conditions. The next day, the cells dispersed from the biofilm in the upper supernatant were collected, counted by a hemocytometer and maintained in SD media or stored in 30% glycerol for further use.

Sample Preparation:

Sampleprep ID:	SP003748
Sampleprep Summary:	C. auris dispersed cell were allowed to grow in 250-mL Erlenmeyer flasks containing 50 mL of SD broth media for 3 days at 30°C. Following incubation, the supernatant was separated by centrifugation for 20 min at 4000 rpm. The resulting supernatant was extracted with 100 mL ethyl acetate. After the extraction step, the organic phases were dried on anhydrous MgSO4, filtered, and concentrated under reduced pressure. The parent C. auris planktonic cells served as control.

Sampleprep ID:

SP003748

Sampleprep Summary:

C. auris dispersed cell were allowed to grow in 250-mL Erlenmeyer flasks containing 50 mL of SD broth media for 3 days at 30°C. Following incubation, the supernatant was separated by centrifugation for 20 min at 4000 rpm. The resulting supernatant was extracted with 100 mL ethyl acetate. After the extraction step, the organic phases were dried on anhydrous MgSO4, filtered, and concentrated under reduced pressure. The parent C. auris planktonic cells served as control.

Combined analysis:

Analysis ID	AN005937
Analysis type	MS
Chromatography type	GC
Chromatography system	Agilent 7890B
Column	Agilent DB5-MS (15m x 0.25mm, 0.25um)
MS Type	EI
MS instrument type	GC-MS
MS instrument name	Agilent 7890B
Ion Mode	POSITIVE
Units	Area Pct

Chromatography:

Chromatography ID:	CH004512
Instrument Name:	Agilent 7890B
Column Name:	Agilent DB5-MS (15m x 0.25mm, 0.25um)
Column Temperature:	300°C
Flow Gradient:	nothing
Flow Rate:	nothing
Solvent A:	nothing
Solvent B:	nothing
Chromatography Type:	GC

MS:

MS ID:	MS005654
Analysis ID:	AN005937
Instrument Name:	Agilent 7890B
Instrument Type:	GC-MS
MS Type:	EI
MS Comments:	The sample analysis began with the column held at 60°C for 5 minutes post-injection. The temperature was subsequently increased to 300°C at a rate of 20°C per minute, followed by a 5-minute hold. The injection was performed in split mode with a split ratio of 5:1 at 300°C. The MS scan range was 50–550 atomic mass units (AMU) under electron impact ionization (70 eV), with a solvent delay of 8.0 minutes. The constituents were identified by mass fragmentations using the NIST mass spectral search program for the NIST/EPA/NIH mass spectral library Version 2.2 (Jun 2014).
Ion Mode:	POSITIVE