Summary of Study ST004042
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002534. The data can be accessed directly via it's Project DOI: 10.21228/M83G11 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST004042 |
| Study Title | Untargeted Metabolomics Identifies Oncometabolites Induced by P2-HNF4α Overexpression in Hepatocytes |
| Study Summary | This study explores the metabolic impact of P2-HNF4α overexpression in primary mouse hepatocytes. Using untargeted metabolomics, we compared metabolite profiles between control and P2-HNF4α-overexpressing cells to identify metabolic pathways potentially altered in liver disease and HCC. |
| Institute | University of Tennessee Health Science Center |
| Department | IMM |
| Laboratory | Mahan Lab |
| Last Name | Fekry |
| First Name | Baharan |
| Address | 1825 Pressler St, Houston TX 77030. USA. |
| Baharan.Fekry@uth.tmc.edu | |
| Phone | 18434693199 |
| Submit Date | 2025-07-03 |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-08-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002534 |
| Project DOI: | doi: 10.21228/M83G11 |
| Project Title: | Untargeted metabolomics analysis on mouse hepatocytes |
| Project Summary: | To determine whether specific metabolites are associated with the induction of P2-HNF4α in liver disease and hepatocellular carcinoma (HCC), we performed untargeted metabolomics on hepatocytes isolated from wild-type mouse liver, which predominantly express the tumor-suppressive P1-HNF4α isoform. Using lentiviral transduction, we overexpressed the P2-HNF4α isoform and conducted metabolite profiling on cell lysates from both control and P2-HNF4α-overexpressing cells. Data analysis was performed using MetaboAnalyst (v5.0), resulting in a list of approximately 120 matched compounds representing significantly predicted metabolites. This study aims to uncover metabolic signatures associated with P2-HNF4α expression, offering insights into its potential role in liver pathogenesis and tumor progression. |
| Institute: | University of Texas Health Science Center at Houston |
| Department: | IMM |
| Laboratory: | Mahan Lab |
| Last Name: | Fekry |
| First Name: | Baharan |
| Address: | 1825 Pressler St, Houston TX 77030. USA, |
| Email: | Baharan.Fekry@uth.tmc.edu |
| Phone: | 7135002487 |
Subject:
| Subject ID: | SU004188 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: Cultured cells; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Treatment |
|---|---|---|---|
| SA467280 | HEP_6FP1 | wildtype | GFP |
| SA467281 | HEP_6FP2 | wildtype | GFP |
| SA467282 | HEP_6FP3 | wildtype | GFP |
| SA467283 | HEP_A8_1 | wildtype | P2_HNF4a |
| SA467284 | HEP_A8_2 | wildtype | P2_HNF4a |
| SA467285 | HEP_A8_3 | wildtype | P2_HNF4a |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO004181 |
| Collection Summary: | Hepatocytes were isolated from mouse livers and transduced in culture with either GFP or P2-HNF4α. Following treatment, cells were collected and subjected to untargeted metabolomics analysis. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR004197 |
| Treatment Summary: | Primary hepatocytes were isolated from mouse livers and cultured in vitro. Cells were transduced with either control GFP or P2-HNF4α-expressing lentivirus. Following 48 hours of transduction, cells were harvested and processed for untargeted metabolomics analysis. |
Sample Preparation:
| Sampleprep ID: | SP004194 |
| Sampleprep Summary: | Samples were diluted in a solution containing 25% acetonitrile, 75% water, and 0.1% formic acid prior to LC-MS analysis. The diluted samples were injected into an Agilent 1200 Series HPLC system coupled to an Agilent 6538 UHD Accurate-Mass Q-TOF mass spectrometer. Chromatographic separation was performed using an Agilent ZORBAX Extend-C18 Rapid Resolution HT column (2.1 × 50 mm, 1.8 µm) under a 20-minute gradient of 25% to 90% Buffer B (80% acetonitrile, 20% water, 0.1% formic acid) at a flow rate of 0.2 mL/min. |
Chromatography:
| Chromatography ID: | CH005077 |
| Chromatography Summary: | Liquid chromatography was performed using an Agilent 1200 Series HPLC system equipped with an Agilent ZORBAX Extend-C18 Rapid Resolution HT column (2.1 × 50 mm, 1.8 µm). The mobile phases consisted of Buffer A (2% acetonitrile, 98% water, 0.1% formic acid) and Buffer B (80% acetonitrile, 20% water, 0.1% formic acid). A linear gradient from 25% to 90% Buffer B was applied over 20 minutes at a flow rate of 0.2 mL/min to achieve metabolite separation prior to MS analysis. |
| Instrument Name: | Agilent 1200 Series HPLC coupled to Agilent 6538 UHD Accurate-Mass Q-TOF |
| Column Name: | Agilent ZORBAX Extend-C18 Rapid Resolution HT (2.1 × 50 mm, 1.8 µm) |
| Column Temperature: | 21°C |
| Flow Gradient: | 25% to 90% Solvent B over 20 minutes |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | 2% Acetonitrile/98% Water; 0.1% formic acid |
| Solvent B: | 80% Acetonitrile/20% Water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN006681 |
| Analysis Type: | MS |
| Chromatography ID: | CH005077 |
| Num Factors: | 2 |
| Num Metabolites: | 356 |
| Units: | LC/MS Peak intensity |
