Metabolite_name RefMet_name HSC_sot_ND_1 HSC_sot_ND_2 HSC_sot_ND_3 HSC_sot_ND_0h_1 HSC_sot_ND_0h_2 HSC_sot_ND_0h_3 HSC_sot_ND_12h_1 HSC_sot_ND_12h_2 HSC_sot_ND_12h_3 HSC_sot_ND_6h_1 HSC_sot_ND_6h_2 HSC_sot_ND_6h_3 HSC_sot_NR_1 HSC_sot_NR_2 HSC_sot_NR_3 HSC_sot_NR_0h_1 HSC_sot_NR_0h_2 HSC_sot_NR_0h_3 HSC_sot_NR_12h_1 HSC_sot_NR_12h_2 HSC_sot_NR_12h_3 HSC_sot_NR_6h_1 HSC_sot_NR_6h_2 HSC_sot_NR_6h_3 Factors - Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 1 h | Batch:1e | Note:[13C, 15N]AAs were used for AA uptake (FigS6.J) Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 1 h | Batch:1e | Note:[13C, 15N]AAs were used for AA uptake (FigS6.J) Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 1 h | Batch:1e | Note:[13C, 15N]AAs were used for AA uptake (FigS6.J) Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:Ctrl, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 1 h | Batch:1e | Note:[13C, 15N]AAs were used for AA uptake (FigS6.J) Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 1 h | Batch:1e | Note:[13C, 15N]AAs were used for AA uptake (FigS6.J) Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 1 h | Batch:1e | Note:[13C, 15N]AAs were used for AA uptake (FigS6.J) Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 0 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 12 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). Source_Name[gating]:Lin– Scal1+ c-Kit+ CD48– CD150+ (HSC) | Treatment:NR 1 week, [13C, 15N] AAs were incorporated for 24 h and subsequently washed out for 6 h | Batch:1e | Note:[13C, 15N] AA levels and [13C]TCA substrate levels in different time point were calculated for AA catabolism (Fig5.L). [13C_ 15N]Alanine_ 2TBDMS Alanine 331000 317000 310000 3310000 3170000 3100000 875000 921000 830000 1600000 1410000 1360000 332000 299000 312000 3320000 2990000 3120000 753000 727000 691000 1330000 1240000 1290000 [13C_ 15N]Aspartic acid_ 3TBDMS Aspartic acid 82500 83500 74300 825000 835000 743000 365000 321000 333000 599000 435000 509000 84000 76900 79700 840000 769000 797000 313000 303000 296000 543000 486000 423000 [13C_ 15N]Glutamic acid_ 3TBDMS Glutamic acid 61300 63800 60700 613000 638000 607000 235000 244000 207000 395000 407000 385000 66700 57100 59100 667000 571000 591000 184000 182000 154000 310000 279000 288000 [13C_ 15N]Glycine_ 2TBDMS Glycine 51100 63400 50800 6350000 5500000 5380000 3040000 3010000 2070000 3840000 4870000 4100000 58700 59500 57400 6710000 5780000 5350000 3130000 2930000 3380000 3880000 4920000 4900000 [13C_ 15N]Histidine_ 3TBDMS Histidine 64800 56500 57700 648000 565000 577000 173000 127000 208000 314000 313000 354000 54600 54400 49500 546000 544000 495000 102000 136000 138000 221000 314000 242000 [13C_ 15N]Isoleucine_ 2TBDMS Isoleucine 328000 306000 281000 3280000 3060000 2810000 1400000 1430000 1100000 2150000 2250000 2140000 296000 384000 357000 2960000 3840000 3570000 1370000 1040000 995000 2270000 2300000 2130000 [13C_ 15N]Leucine_ 2TBDMS Leucine 369000 353000 318000 3690000 3530000 3180000 1040000 984000 1080000 2120000 2240000 2140000 302000 353000 321000 3020000 3530000 3210000 782000 810000 741000 1460000 1580000 1900000 [13C_ 15N]Lysine_ 3TBDMS Lysine 148000 139000 133000 1480000 1390000 1330000 594000 551000 585000 813000 786000 796000 134000 142000 166000 1340000 1420000 1660000 466000 441000 464000 766000 780000 837000 [13C_ 15N]Methionine_ 2TBDMS Methionine 197000 206000 182000 1970000 2060000 1820000 740000 680000 544000 1200000 1180000 927000 200000 220000 173000 2000000 2200000 1730000 501000 575000 607000 1010000 1270000 1040000 [13C_ 15N]Phenylalanine_ 2TBDMS Phenylalanine 289000 297000 243000 2890000 2970000 2430000 1240000 1380000 1130000 1410000 1520000 1440000 246000 276000 222000 2460000 2760000 2220000 1000000 929000 926000 1360000 1240000 1360000 [13C_ 15N]Proline_ 2TBDMS Proline 184000 168000 174000 1840000 1680000 1740000 450000 528000 423000 866000 829000 857000 176000 161000 160000 1760000 1610000 1600000 319000 275000 325000 750000 701000 731000 [13C_ 15N]Serine_ 3TBDMS Serine 321000 324000 282000 3210000 3240000 2820000 964000 851000 886000 2120000 2190000 1860000 347000 311000 356000 3470000 3110000 3560000 712000 793000 822000 1940000 1880000 1650000 [13C_ 15N]Threonine_ 3TBDMS Threonine 86000 79700 77200 860000 797000 772000 342000 313000 322000 510000 508000 413000 61200 67200 85200 612000 672000 852000 286000 244000 232000 362000 350000 368000 [13C_ 15N]Tyrosine_ 3TBDMS Tyrosine 798000 774000 724000 7980000 7740000 7240000 2250000 2370000 2940000 4140000 4380000 3180000 730000 698000 740000 7300000 6980000 7400000 2210000 1830000 1850000 3120000 3970000 3320000 [13C_ 15N]Valine_ 2TBDMS Valine 805000 762000 793000 8050000 7620000 7930000 3210000 2980000 2570000 5270000 4440000 4280000 822000 769000 787000 8220000 7690000 7870000 1790000 2280000 2610000 4020000 4230000 4080000 [13C]Alpha_Ketoglutarate 2-Oxoglutaric acid 2160000 1880000 2000000 34000 37400 41200 26100 28500 30100 23500 20500 21700 50900 51500 50900 33400 32100 35000 [13C]Citrate Citric acid 45400 42100 39700 81800 80700 86800 70200 69300 69800 48900 55500 46700 107000 90600 95600 82200 84200 82400 [13C]Fumatate Fumaric acid 173000 176000 142000 343000 315000 342000 244000 249000 275000 159000 138000 138000 364000 354000 367000 293000 276000 285000 [13C]Malate Malic acid 84900 90700 104000 142000 162000 145000 116000 136000 133000 108000 137000 119000 164000 178000 184000 141000 150000 156000 [13C]Succinate Succinic acid 128000 135000 113000 213000 235000 231000 192000 160000 185000 108000 111000 123000 247000 246000 267000 209000 192000 206000