metabolite_name NecJ 4h 1 NecJ 4h 2 NecJ 4h 3 NecJ 4h 1- NecJ 4h 2- NecJ 4h 3- NecJ 4h 1+ NecJ 4h 2+ NecJ 4h 3+ NecJ 8h 1 NecJ 8h 2 NecJ 8h 3 NecJ 8h 1- NecJ 8h 2- NecJ 8h 3- NecJ 8h 1+ NecJ 8h 2+ NecJ 8h 3+ L-alanine 5.37E+05 4.25E+05 8.15E+05 4.11E+05 6.16E+05 3.13E+05 3.17E+05 4.00E+05 6.74E+05 4.85E+05 5.50E+05 3.90E+05 4.38E+05 5.73E+05 3.77E+05 6.75E+05 6.89E+05 5.70E+05 L-arginine 1.69E+05 1.83E+05 2.27E+05 3.12E+05 2.41E+05 2.09E+05 2.49E+06 2.97E+06 3.09E+06 1.79E+05 1.20E+05 2.09E+05 2.05E+05 2.33E+05 3.52E+05 1.42E+06 1.73E+06 1.54E+06 L-asparagine 2.14E+04 4.10E+04 2.77E+04 2.70E+04 5.60E+04 0.00E+00 1.99E+05 1.45E+05 1.05E+05 4.48E+04 0.00E+00 0.00E+00 2.13E+04 0.00E+00 2.78E+01 7.51E+04 1.11E+05 1.10E+05 L-aspartate 1.71E+05 1.24E+05 1.43E+05 1.57E+05 1.73E+05 1.30E+05 4.11E+05 2.94E+05 2.09E+05 3.07E+05 2.73E+05 2.15E+05 1.81E+05 2.10E+05 2.27E+05 3.36E+05 3.76E+05 3.29E+05 L-cysteine 2.51E+05 2.27E+05 2.65E+05 2.45E+05 2.88E+05 2.05E+05 1.64E+06 1.80E+06 1.46E+06 2.07E+05 2.16E+05 1.72E+05 1.95E+05 2.54E+05 2.17E+05 8.91E+05 1.19E+06 1.17E+06 L-glutamate 1.24E+06 1.17E+06 1.09E+06 1.04E+06 1.31E+06 8.97E+05 1.98E+06 1.86E+06 1.35E+06 1.35E+06 1.67E+06 1.43E+06 9.81E+05 1.31E+06 1.25E+06 1.43E+06 1.87E+06 1.80E+06 L-glutamine 5.01E+05 4.46E+05 5.49E+05 4.34E+05 5.68E+05 4.51E+05 2.30E+06 2.11E+06 1.78E+06 4.36E+05 3.91E+05 3.24E+05 3.01E+05 4.49E+05 4.08E+05 1.08E+06 1.53E+06 1.47E+06 glycine 2.42E+06 1.52E+06 1.43E+06 1.62E+06 1.42E+06 3.77E+06 3.48E+06 2.45E+06 1.92E+06 3.62E+06 1.71E+06 3.70E+06 2.12E+06 1.96E+06 2.47E+06 1.35E+06 1.93E+06 2.85E+06 L-leucine 8.79E+05 1.00E+06 1.16E+06 9.79E+05 9.72E+05 1.14E+06 6.02E+06 5.97E+06 4.28E+06 1.41E+06 5.88E+05 8.13E+05 7.10E+05 8.11E+05 1.79E+06 2.04E+06 2.77E+06 2.73E+06 L-lysine 7.62E+04 1.04E+05 1.69E+05 1.97E+05 1.98E+05 1.08E+05 1.81E+06 2.18E+06 2.06E+06 8.54E+04 8.33E+04 7.07E+04 1.94E+05 1.58E+05 2.49E+05 1.22E+06 1.27E+06 1.34E+06 L-methionine 5.07E+04 4.59E+04 4.45E+04 4.54E+04 6.17E+04 5.59E+04 6.99E+05 6.72E+05 5.20E+05 4.73E+04 4.51E+04 4.06E+04 5.55E+04 6.18E+04 5.37E+04 2.55E+05 3.82E+05 3.83E+05 L-phenylalanine 9.00E+04 1.03E+05 9.61E+04 9.05E+04 8.47E+04 1.11E+05 3.78E+05 3.02E+05 2.43E+05 1.69E+05 7.83E+04 8.36E+04 5.70E+04 8.81E+04 2.00E+05 1.46E+05 1.78E+05 2.03E+05 L-proline 3.01E+06 3.57E+06 2.77E+06 2.48E+06 3.35E+06 3.21E+06 7.20E+06 8.40E+06 6.91E+06 3.01E+06 2.34E+06 2.75E+06 2.07E+06 2.25E+06 3.56E+06 3.62E+06 5.32E+06 5.01E+06 L-serine 1.15E+04 3.92E+04 1.06E+05 6.59E+04 4.69E+04 6.62E+04 2.16E+05 1.69E+05 1.00E+05 1.07E+05 3.24E+03 8.49E+03 3.11E+04 2.49E+03 1.83E+05 1.13E+03 1.15E+05 1.19E+05 L-threonine 9.71E+04 1.23E+05 1.71E+05 1.11E+05 1.54E+05 1.19E+05 5.86E+05 5.28E+05 4.40E+05 1.56E+05 8.66E+04 1.70E+05 1.04E+05 1.43E+05 2.43E+05 2.73E+05 3.77E+05 3.60E+05 L-tryptophan 2.24E+05 4.11E+04 4.06E+04 5.35E+04 8.52E+04 1.15E+05 9.29E+04 6.09E+04 7.16E+04 8.47E+04 2.22E+05 4.35E+04 1.70E+05 4.36E+04 1.23E+05 1.11E+05 3.63E+05 1.19E+05 L-tyrosine 1.43E+05 1.48E+05 2.79E+05 1.72E+05 1.51E+05 2.02E+05 6.72E+05 5.59E+05 4.45E+05 2.61E+05 7.35E+04 2.11E+05 1.24E+05 1.66E+05 3.68E+05 2.15E+05 3.67E+05 3.45E+05 L-valine 5.56E+06 5.22E+06 5.30E+06 4.96E+06 5.09E+06 4.13E+06 5.76E+06 5.38E+06 5.04E+06 3.26E+06 3.03E+06 3.42E+06 2.70E+06 2.76E+06 3.62E+06 2.69E+06 3.52E+06 3.75E+06 AMP 2.90E+06 5.65E+05 1.34E+06 8.76E+05 6.35E+05 5.36E+05 1.22E+06 1.07E+06 4.25E+05 7.22E+05 5.52E+05 3.75E+05 6.61E+05 1.94E+06 2.29E+05 7.12E+05 8.08E+05 1.13E+06 Adenosine 8.86E+05 4.65E+05 4.05E+05 5.69E+05 3.73E+05 5.73E+05 2.46E+05 2.94E+05 2.08E+05 3.53E+05 3.70E+05 5.87E+05 6.26E+05 3.78E+05 3.90E+05 1.64E+05 2.99E+05 2.41E+05 Adenine 2.94E+05 1.45E+05 1.55E+05 1.83E+05 1.24E+05 1.69E+05 1.23E+05 1.45E+05 1.39E+05 1.49E+05 1.63E+05 2.36E+05 2.25E+05 2.07E+05 1.86E+05 1.09E+05 2.04E+05 1.82E+05 Guanine 5.47E+03 9.24E+03 9.06E+03 2.17E+04 3.30E+04 2.72E+04 3.57E+05 3.87E+05 2.78E+05 0.00E+00 0.00E+00 7.87E+03 4.98E+04 0.00E+00 4.02E+04 1.75E+05 1.95E+05 1.77E+05 CMP 2.50E+04 2.61E+04 1.84E+04 1.84E+04 1.69E+04 7.34E+03 6.04E+04 5.37E+04 3.84E+04 2.56E+04 2.76E+04 2.51E+04 2.29E+04 2.62E+04 6.31E+03 3.07E+04 1.93E+04 3.70E+04 Cytidine 1.34E+05 1.07E+05 1.25E+05 2.32E+05 7.84E+04 2.22E+04 4.03E+05 3.58E+05 3.13E+05 7.99E+04 4.98E+04 8.73E+04 5.37E+04 5.94E+04 1.16E+05 3.52E+05 3.46E+05 3.03E+05 Cytosine 5.92E+04 4.81E+04 7.02E+04 2.00E+05 2.60E+05 2.21E+05 4.31E+06 4.37E+06 3.81E+06 6.40E+04 5.29E+04 3.02E+04 5.82E+05 2.99E+05 4.25E+05 3.47E+06 4.02E+06 3.52E+06 Thymine 4.46E+04 2.37E+04 2.15E+04 4.37E+04 3.32E+04 5.20E+04 3.90E+05 4.11E+05 2.99E+05 7.83E+04 4.56E+04 4.72E+04 7.93E+04 4.62E+04 8.40E+04 2.32E+05 3.02E+05 2.54E+05 Hypoxanthine 3.68E+05 1.25E+05 2.16E+05 2.58E+05 2.15E+05 2.18E+05 1.96E+06 1.97E+06 1.24E+06 1.50E+05 9.96E+04 1.05E+05 2.82E+05 2.26E+05 2.13E+05 6.81E+05 9.65E+05 1.13E+06 Allantoate 2.89E+04 7.41E+04 1.23E+05 6.28E+04 7.40E+04 6.48E+04 1.79E+05 2.23E+05 1.94E+05 1.05E+05 3.82E+04 6.01E+04 4.24E+04 7.63E+04 1.56E+05 1.36E+05 1.93E+05 2.30E+05 Nicotinamide 4.25E+06 1.49E+06 2.67E+06 1.38E+06 1.50E+06 2.52E+06 3.52E+06 3.03E+06 1.35E+06 3.31E+06 1.77E+06 2.37E+06 1.98E+06 3.88E+06 1.42E+06 2.03E+06 2.54E+06 3.70E+06 NAD+ 9.15E+04 1.28E+05 1.26E+05 1.39E+05 1.34E+05 1.41E+05 1.78E+05 1.41E+05 1.53E+05 3.08E+05 2.46E+05 2.98E+05 2.47E+05 1.38E+05 2.76E+05 1.99E+05 1.93E+05 1.97E+05 5-Oxoproline 1.15E+06 1.41E+06 1.66E+06 1.28E+06 1.15E+06 1.50E+06 2.38E+06 2.28E+06 1.79E+06 2.33E+06 8.71E+05 1.45E+06 9.36E+05 1.02E+06 3.35E+06 1.31E+06 1.84E+06 1.76E+06 Cys-Gly 6.38E+04 5.83E+04 7.10E+04 5.42E+04 6.73E+04 7.13E+04 1.18E+05 8.23E+04 7.16E+04 1.09E+05 1.02E+05 1.13E+05 6.76E+04 8.27E+04 8.99E+04 1.10E+05 1.12E+05 1.10E+05 gamma-Glutamyl-gamma-aminobutyrate 1.27E+04 2.59E+03 2.53E+04 1.09E+04 1.03E+04 2.25E+04 1.93E+05 1.68E+05 1.32E+05 3.40E+04 2.25E+04 3.45E+04 3.19E+04 1.11E+04 2.78E+04 9.36E+04 1.13E+05 1.27E+05 Dimethylglycine 6.89E+05 6.77E+05 6.60E+05 7.41E+05 6.71E+05 2.88E+05 3.97E+05 6.07E+05 6.67E+05 5.64E+05 6.96E+05 4.35E+05 6.40E+05 6.33E+05 5.86E+05 6.66E+05 5.78E+05 6.48E+05 S-Adenosyl-L-methionine 9.08E+04 8.58E+04 1.29E+05 1.12E+05 1.25E+05 9.33E+04 1.49E+05 1.53E+05 1.53E+05 1.72E+05 1.77E+05 1.67E+05 1.85E+05 1.93E+05 1.76E+05 2.39E+05 2.79E+05 2.37E+05 L-Citrulline 9.93E+05 4.91E+05 8.58E+05 9.33E+05 8.49E+05 6.48E+05 1.49E+06 1.13E+06 8.74E+05 6.69E+05 4.29E+05 4.08E+05 4.59E+05 4.53E+05 6.04E+05 6.66E+05 6.60E+05 6.64E+05 Putrescine 1.35E+06 1.34E+06 1.25E+06 1.07E+06 1.29E+06 1.12E+06 9.95E+05 1.09E+06 1.00E+06 1.38E+06 1.17E+06 9.98E+05 8.11E+05 8.63E+05 7.40E+05 9.50E+05 1.04E+06 1.00E+06 Spermidine 2.62E+07 3.51E+07 3.81E+07 3.11E+07 3.56E+07 3.76E+07 3.16E+07 3.39E+07 4.25E+07 3.59E+07 3.67E+07 4.20E+07 4.23E+07 4.65E+07 5.00E+07 4.44E+07 4.17E+07 4.47E+07 Spermine 1.88E+06 2.16E+06 2.06E+06 1.96E+06 2.63E+06 1.44E+06 2.24E+06 3.29E+06 4.31E+06 1.62E+06 1.69E+06 1.93E+06 2.07E+06 2.09E+06 3.08E+06 3.95E+06 3.70E+06 3.49E+06 Phosphocreatine 4.44E+05 5.30E+05 5.21E+05 3.79E+05 5.25E+05 3.55E+05 2.85E+05 3.76E+05 2.86E+05 1.26E+06 1.34E+06 1.10E+06 8.57E+05 1.08E+06 1.23E+06 7.74E+05 9.57E+05 1.09E+06 Creatine 1.55E+07 9.41E+06 1.03E+07 9.35E+06 1.06E+07 1.21E+07 8.70E+06 6.89E+06 6.67E+06 3.20E+07 2.54E+07 2.59E+07 1.91E+07 1.56E+07 2.12E+07 1.50E+07 1.68E+07 2.15E+07 Creatinine 1.84E+05 1.27E+05 2.03E+05 1.87E+05 1.70E+05 1.67E+05 2.78E+05 2.78E+05 2.55E+05 2.39E+05 1.88E+05 2.21E+05 1.46E+05 1.53E+05 2.69E+05 2.52E+05 3.08E+05 2.78E+05 trans-4-Hydroxy-L-proline 3.88E+05 4.42E+05 3.62E+05 3.16E+05 4.72E+05 2.19E+05 1.97E+05 2.04E+05 1.37E+05 1.84E+05 1.71E+05 1.68E+05 1.06E+05 3.14E+05 2.09E+05 1.18E+05 1.13E+05 1.94E+05 Pantothenol 1.28E+05 1.24E+05 1.11E+05 8.48E+04 1.17E+05 1.48E+05 1.67E+05 1.98E+05 1.46E+05 1.39E+05 1.23E+05 1.87E+05 1.17E+05 1.27E+05 1.91E+05 1.29E+05 1.66E+05 1.94E+05 Taurine 1.40E+06 1.00E+06 1.01E+06 1.09E+06 1.35E+06 8.53E+05 6.92E+05 7.62E+05 5.56E+05 2.08E+06 1.81E+06 1.52E+06 1.32E+06 1.68E+06 1.60E+06 8.27E+05 1.07E+06 9.76E+05 L-Methionine S-oxide 7.00E+03 1.57E+04 7.81E+03 1.39E+04 6.86E+03 1.55E+04 7.27E+04 6.32E+04 6.49E+04 1.79E+04 1.01E+04 6.44E+03 3.58E+03 8.17E+03 2.27E+04 4.65E+04 5.40E+04 4.78E+04 3-methyldioxyindole 2.76E+05 1.19E+05 1.05E+05 9.20E+04 1.33E+05 5.09E+05 4.11E+05 1.91E+05 1.34E+05 3.70E+05 1.12E+05 3.74E+05 1.69E+05 1.52E+05 2.68E+05 9.74E+04 1.63E+05 2.67E+05 Anthranilate 3.05E+05 3.94E+05 5.78E+05 3.01E+05 1.57E+05 3.86E+05 3.25E+05 2.39E+05 2.02E+05 6.99E+05 1.24E+05 1.40E+05 1.81E+05 1.16E+05 1.04E+06 1.79E+05 1.50E+05 2.78E+05 Picolinic acid 1.90E+05 6.47E+04 5.03E+04 6.05E+04 8.66E+04 3.91E+05 2.37E+05 1.64E+05 1.12E+05 2.36E+05 9.67E+04 2.67E+05 1.12E+05 9.17E+04 1.90E+05 5.83E+04 1.62E+05 1.37E+05 Acetylcholine 3.52E+06 3.82E+06 3.49E+06 2.83E+06 3.50E+06 3.48E+06 3.23E+06 4.07E+06 3.72E+06 3.55E+06 3.57E+06 3.70E+06 3.89E+06 3.89E+06 4.09E+06 3.46E+06 3.82E+06 4.94E+06 Choline 7.38E+07 8.83E+07 8.23E+07 7.09E+07 8.38E+07 7.78E+07 7.36E+07 8.85E+07 9.14E+07 1.05E+08 1.04E+08 9.83E+07 1.19E+08 1.27E+08 1.17E+08 1.11E+08 1.15E+08 1.12E+08 Triacanthine 4.42E+07 4.15E+07 4.03E+07 3.47E+07 3.85E+07 3.82E+07 2.95E+07 3.95E+07 3.12E+07 4.68E+07 4.03E+07 4.90E+07 4.70E+07 3.88E+07 4.30E+07 3.04E+07 3.32E+07 3.56E+07 6-Lactoyl-5-6-7-8-tetrahydropterin 4.08E+05 3.49E+05 4.08E+05 3.34E+05 3.81E+05 3.61E+05 5.45E+05 5.56E+05 4.51E+05 5.18E+05 5.26E+05 5.39E+05 4.20E+05 4.81E+05 3.76E+05 5.25E+05 7.02E+05 7.00E+05 Ergothioneine 9.91E+04 6.02E+04 6.53E+04 6.98E+04 5.95E+04 6.96E+04 6.87E+04 7.36E+04 7.22E+04 1.10E+05 7.52E+04 1.02E+05 6.01E+04 7.09E+04 8.93E+04 5.85E+04 7.35E+04 1.01E+05 cis-p-Coumarate 4.36E+04 3.09E+04 6.24E+04 3.25E+04 3.18E+04 5.16E+04 1.48E+05 1.22E+05 9.35E+04 6.03E+04 1.82E+04 5.97E+04 2.21E+04 4.45E+04 8.12E+04 5.42E+04 8.28E+04 9.11E+04 N6-Methyl-L-lysine 3.70E+05 1.64E+05 2.66E+05 2.02E+05 1.45E+05 1.51E+05 2.82E+05 1.78E+05 2.06E+05 2.43E+05 5.98E+04 6.10E+04 9.24E+04 6.67E+04 2.44E+05 1.74E+05 4.29E+05 1.39E+05 Fe(III)dicitrate 3.92E+05 2.85E+05 2.94E+05 2.69E+05 3.12E+05 3.54E+05 3.23E+05 3.37E+05 2.96E+05 3.71E+05 2.99E+05 3.73E+05 3.48E+05 3.26E+05 3.80E+05 2.86E+05 3.22E+05 3.45E+05 L-Noradrenaline 3.66E+04 5.49E+04 5.56E+04 4.34E+04 2.93E+04 7.61E+04 7.93E+04 6.64E+04 8.11E+04 4.50E+04 4.97E+04 3.20E+04 2.05E+04 7.01E+04 3.91E+04 4.74E+04 5.56E+04 5.19E+04 2-Oxo-7-methylthioheptanoic acid 4.35E+04 4.86E+04 5.09E+04 7.97E+04 9.18E+04 7.59E+04 8.49E+05 1.03E+06 9.78E+05 4.83E+04 4.39E+04 2.76E+04 9.35E+04 8.37E+04 1.20E+05 6.05E+05 5.26E+05 5.90E+05 metabolite_name KEGG ID m/z r.t. L-alanine C00041 90.0555 0.40 L-arginine C00062 175.1187 1.19 L-asparagine C00152 133.0607 1.22 L-aspartate C00049 134.0447 1.21 L-cysteine C00097 122.0271 1.22 L-glutamate C00025 148.0601 1.24 L-glutamine C00064 147.0762 1.20 glycine C00037 76.0400 1.27 L-leucine C00123 132.1017 1.25 L-lysine C00047 147.1126 1.11 L-methionine C00073 150.0581 1.24 L-phenylalanine C00079 166.0860 1.28 L-proline C00148 116.0708 1.23 L-serine C00065 106.0503 1.21 L-threonine C00188 120.0656 1.20 L-tryptophan C00078 205.0967 1.33 L-tyrosine C00082 182.0809 1.23 L-valine C00183 118.0864 1.22 AMP C00020 348.0691 1.24 Adenosine C00212 268.1030 1.25 Adenine C00147 136.0616 1.29 Guanine C00242 152.0565 1.25 CMP C00055 324.0580 1.23 Cytidine C00475 244.0944 1.26 Cytosine C00380 112.0508 1.24 Thymine C00178 127.0502 1.24 Hypoxanthine C00262 137.0456 1.26 Allantoate C00499 177.0606 1.14 Nicotinamide C00153 123.0553 1.36 NAD+ C00003 664.1138 1.24 5-Oxoproline C01879 130.0498 1.22 Cys-Gly C01419 179.0480 1.24 gamma-Glutamyl-gamma-aminobutyrate C15767 233.1131 1.21 Dimethylglycine C01026 104.0709 1.43 S-Adenosyl-L-methionine C00019 399.1429 1.20 L-Citrulline C00327 176.1026 1.21 Putrescine C00134 89.1079 1.12 Spermidine C00315 146.1650 1.10 Spermine C00750 203.2226 1.04 Phosphocreatine C02305 212.0425 1.30 Creatine C00300 132.0766 1.23 Creatinine C00791 114.0663 1.22 trans-4-Hydroxy-L-proline C01157 132.0654 1.26 Pantothenol C00864 220.1173 1.35 Taurine C00245 126.0220 1.23 L-Methionine S-oxide C02989 166.0528 1.22 3-methyldioxyindole C05834 164.0703 1.48 Anthranilate C00108 138.0547 1.27 Picolinic acid C10164 124.0394 1.39 Acetylcholine C01996 146.1172 1.24 Choline C00114 104.1073 1.20 Triacanthine C08435 204.1225 1.25 6-Lactoyl-5-6-7-8-tetrahydropterin C04244 240.1083 1.21 Ergothioneine C02732 230.0951 1.24 cis-p-Coumarate C06738 165.0542 1.25 N6-Methyl-L-lysine C02728 161.1281 1.24 Fe(III)dicitrate C06229 245.9443 1.45 L-Noradrenaline C00547 170.0809 1.26 2-Oxo-7-methylthioheptanoic acid C17220 191.0761 1.14