#METABOLOMICS WORKBENCH csclendinen_20140828_9344901_mwtab.txt DATATRACK_ID:148 TEXT OUTPUT STUDY_ID:ST000097 ANALYSIS_ID:AN000156 PROJECT_ID:PR000089 VERSION 1 CREATED_ON 10-02-2015 #PROJECT PR:PROJECT_TITLE 13C Metabolomics: Applications at Natural Abundance PR:INSTITUTE University of Florida PR:DEPARTMENT Department of Biochemistry and Molecular Biology PR:LABORATORY Edison PR:LAST_NAME Edison PR:FIRST_NAME Arthur PR:ADDRESS R3-226 Academic Research Building, Department of Biochemistry & Molecular PR:ADDRESS Biology, PO Box 100245, Gainesville, FL 32610-0245 PR:EMAIL aedison@ufl.edu PR:PHONE 352-392-4535 PR:PROJECT_SUMMARY - #STUDY ST:STUDY_TITLE NMR analysis of D. melanogaster ST:STUDY_TYPE Cold vs Hardy ST:STUDY_SUMMARY Twenty replicates of each genetic line (cold hardy and cold tolerant) were ST:STUDY_SUMMARY collected with 40 flies per replicate. ST:INSTITUTE University of Florida ST:DEPARTMENT Department of Biochemistry and Molecular Biology ST:LABORATORY Arthur Edison ST:LAST_NAME Chaevien ST:FIRST_NAME Clendinen ST:ADDRESS R3-226 Academic Research Building, Department of Biochemistry & Molecular ST:ADDRESS Biology, PO Box 100245, Gainesville, FL 32610-0245 ST:EMAIL csclendinen@ufl.edu ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 40 ST:PHONE - #SUBJECT SU:SUBJECT_TYPE Animals SU:SUBJECT_SPECIES Drosophila melanogaster SU:TAXONOMY_ID 7227 SU:GENDER Female SU:SPECIES_GROUP Insect #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - H2_1 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_10 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_11 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_12 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_13 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_14 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_15 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_16 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_17 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_18 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_19 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_2 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_20 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_3 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_4 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_5 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_6 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_7 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_8 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - H2_9 Cold Susceptibility:Cold Hardy SUBJECT_SAMPLE_FACTORS - L2_1 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_10 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_11 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_12 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_13 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_14 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_15 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_16 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_17 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_18 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_19 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_2 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_20 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_3 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_4 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_5 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_6 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_7 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_8 Cold Susceptibility:Cold Susceptible SUBJECT_SAMPLE_FACTORS - L2_9 Cold Susceptibility:Cold Susceptible #COLLECTION CO:COLLECTION_SUMMARY Polar-phase metabolites were extracted from homogenized whole fly tissue using CO:COLLECTION_SUMMARY an aqueous-optimized metabolite extraction protocol. (Wu, H.; Southam, A. D.; CO:COLLECTION_SUMMARY Hines, A.; Viant, M. R. Analytical biochemistry 2008.) CO:SAMPLE_TYPE Tissue extract #TREATMENT TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Twenty replicates of each genetic line (cold hardy and cold tolerant) were SP:SAMPLEPREP_SUMMARY collected with 40 flies per replicate. Polar phase extracted metabolites were SP:SAMPLEPREP_SUMMARY dried and reconstituted in 40 ?L of 99% D2O and pipetted into 1.5-mm NMR tubes. SP:PROCESSING_METHOD Lyophilization SP:PROCESSING_STORAGE_CONDITIONS -80 degree celsius SP:EXTRACTION_METHOD Polar phase extraction #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE - CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:LABORATORY_NAME Edison AN:ANALYSIS_TYPE NMR AN:SOFTWARE_VERSION VNMRJ AN:OPERATOR_NAME Chaevien Clendinen AN:DATA_FORMAT Varian #NMR NM:INSTRUMENT_TYPE Varian NM:NMR_EXPERIMENT_TYPE 1D 1H NM:SPECTROMETER_FREQUENCY 600 MHz NM:NMR_PROBE 1.5 mm HTS cryoprobe NM:NMR_SOLVENT D2O NM:NMR_TUBE_SIZE 1.5 mm NM:SHIMMING_METHOD Manual NM:PULSE_SEQUENCE 1D 1H NM:WATER_SUPPRESSION Presaturation NM:PULSE_WIDTH 45 NM:RECEIVER_GAIN 28 NM:OFFSET_FREQUENCY 4.77 NM:CHEMICAL_SHIFT_REF_CPD TSP NM:TEMPERATURE 22 degree Celsius NM:NUMBER_OF_SCANS 64 NM:DUMMY_SCANS 16 NM:ACQUISITION_TIME 2.3s NM:RELAXATION_DELAY 2s NM:SPECTRAL_WIDTH 12 ppm NM:NUM_DATA_POINTS_ACQUIRED 32768 NM:REAL_DATA_POINTS 16384 NM:ZERO_FILLING 2x NM:APODIZATION Cosine squared NM:INSTRUMENT_NAME Varian #NMR_BINNED_DATA NMR_BINNED_DATA:UNITS Peak area NMR_BINNED_DATA_START Bin range(ppm) H2_1 H2_10 H2_11 H2_12 H2_13 H2_14 H2_15 H2_16 H2_17 H2_18 H2_19 H2_2 H2_20 H2_3 H2_4 H2_5 H2_6 H2_7 H2_8 H2_9 L2_1 L2_10 L2_11 L2_12 L2_13 L2_14 L2_15 L2_16 L2_17 L2_18 L2_19 L2_2 L2_20 L2_3 L2_4 L2_5 L2_6 L2_7 L2_8 L2_9 NMR_BINNED_DATA_END #END