{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000099","ANALYSIS_ID":"AN000163","VERSION":"1","CREATED_ON":"10-02-2015"},

"PROJECT":{"PROJECT_TITLE":"13C Metabolomics: Applications at Natural Abundance","INSTITUTE":"University of Florida","DEPARTMENT":"Department of Biochemistry and Molecular Biology","LABORATORY":"Edison","LAST_NAME":"Edison","FIRST_NAME":"Arthur","ADDRESS":"R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245","EMAIL":"aedison@ufl.edu","PHONE":"352-392-4535","PROJECT_SUMMARY":"-"},

"STUDY":{"STUDY_TITLE":"NMR analysis of DMD mouse serum","STUDY_SUMMARY":"Measurements were performed on serum using 6 month old C57/B10 control (n=6) and mdx (n=8) mice. 1D proton and carbon spectra were collected on these samples","INSTITUTE":"University of Florida","DEPARTMENT":"Department of Biochemistry and Molecular Biology / SECIM","LABORATORY":"Arthur Edison","LAST_NAME":"Edison","FIRST_NAME":"Arthur","ADDRESS":"R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245","EMAIL":"aedison@ufl.edu","NUM_GROUPS":"2","TOTAL_SUBJECTS":"14","PHONE":"-"},

"SUBJECT":{"SUBJECT_TYPE":"animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57/BL/10","AGE_OR_AGE_RANGE":"6 month old","GENDER":"males","SPECIES_GROUP":"Mammal"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"Mouse Serum",
"Sample ID":"295302_m1",
"Factors":{"Strain":"C57/BL/10ScSn-Dmdmdx/J","model":"mdx"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"295302_m2",
"Factors":{"Strain":"C57/BL/10ScSn-Dmdmdx/J","model":"mdx"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"295302_m3",
"Factors":{"Strain":"C57/BL/10ScSn-Dmdmdx/J","model":"mdx"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"295302_m4",
"Factors":{"Strain":"C57/BL/10ScSn-Dmdmdx/J","model":"mdx"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"295303_m2",
"Factors":{"Strain":"C57/BL/10ScSn-Dmdmdx/J","model":"mdx"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"295303_m3",
"Factors":{"Strain":"C57/BL/10ScSn-Dmdmdx/J","model":"mdx"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"295303_m4",
"Factors":{"Strain":"C57/BL/10ScSn-Dmdmdx/J","model":"mdx"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"295303_m5",
"Factors":{"Strain":"C57/BL/10ScSn-Dmdmdx/J","model":"mdx"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"298004_m1",
"Factors":{"Strain":"C57/BL/10SnJ","model":"Control"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"298004_m2",
"Factors":{"Strain":"C57/BL/10SnJ","model":"Control"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"298004_m3",
"Factors":{"Strain":"C57/BL/10SnJ","model":"Control"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"298005_m2",
"Factors":{"Strain":"C57/BL/10SnJ","model":"Control"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"298005_m3",
"Factors":{"Strain":"C57/BL/10SnJ","model":"Control"}
},
{
"Subject ID":"Mouse Serum",
"Sample ID":"298005_m4",
"Factors":{"Strain":"C57/BL/10SnJ","model":"Control"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Mouse serum measurements were performed on 6 replicates of 6 month old C57/B10 control and 8 replicates of mdx mice. Two hundred ?L of blood was collected by submandibular puncture, clotted and centrifuged to extract serum. The mice were then humanely euthanized under 2% isofluorene  anesthesia. Serum was flash frozen in liquid nitrogen and stored at -80 °C.","SAMPLE_TYPE":"BLOOD","COLLECTION_METHOD":"submandibular puncture,","COLLECTION_LOCATION":"University of Florida","COLLECTION_FREQUENCY":"1","VOLUMEORAMOUNT_COLLECTED":"200 microliters","STORAGE_CONDITIONS":"-80 degree celsius","BLOOD_SERUM_OR_PLASMA":"BLOOD Serum"},

"TREATMENT":{"ANIMAL_ENDP_EUTHANASIA":"2% Isofluorene","TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Approximately 100 ?L of serum was lyophilized and re-suspended in 40 ?L 99% D2O and transferred into 1.5 mm NMR tubes (Norell).","PROCESSING_METHOD":"lyophilized","PROCESSING_STORAGE_CONDITIONS":"-80 degree celsius","EXTRACTION_METHOD":"submandibular puncture"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"-","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},

"ANALYSIS":{"LABORATORY_NAME":"Edison","ANALYSIS_TYPE":"NMR","SOFTWARE_VERSION":"VNMRJ","OPERATOR_NAME":"Brittany Lee McMullen","DATA_FORMAT":"Varian"},

"NM":{"INSTRUMENT_TYPE":"Varian","NMR_EXPERIMENT_TYPE":"1D 1H","SPECTROMETER_FREQUENCY":"600 MHz","NMR_PROBE":"1.5 mm HTS cryoprobe","NMR_SOLVENT":"D2O","NMR_TUBE_SIZE":"1.5 mm","SHIMMING_METHOD":"Manual","PULSE_SEQUENCE":"1D 1H CPMG","WATER_SUPPRESSION":"Presaturation","PULSE_WIDTH":"45","RECEIVER_GAIN":"28","OFFSET_FREQUENCY":"4.77","CHEMICAL_SHIFT_REF_CPD":"TSP","TEMPERATURE":"22 degree Celsius","NUMBER_OF_SCANS":"256","DUMMY_SCANS":"8","ACQUISITION_TIME":"2.0s","RELAXATION_DELAY":"2s","SPECTRAL_WIDTH":"16 ppm","NUM_DATA_POINTS_ACQUIRED":"38422","REAL_DATA_POINTS":"19211","ZERO_FILLING":"2x","APODIZATION":"Cosine squared","CHEMICAL_SHIFT_REF_STD":"TSP","INSTRUMENT_NAME":"Varian"},

"Data":[{"Bin range(ppm)":"NMR_BINNED_DATA_END","295302_m1":"","295302_m2":"","295302_m3":"","295302_m4":"","295303_m2":"","295303_m3":"","295303_m4":"","295303_m5":"","298004_m1":"","298004_m2":"","298004_m3":"","298005_m2":"","298005_m3":"","298005_m4":""},{"Bin range(ppm)":"#END","295302_m1":"","295302_m2":"","295302_m3":"","295302_m4":"","295303_m2":"","295303_m3":"","295303_m4":"","295303_m5":"","298004_m1":"","298004_m2":"","298004_m3":"","298005_m2":"","298005_m3":"","298005_m4":""}]

}