#METABOLOMICS WORKBENCH csclendinen_20140910_9007141_mwtab.txt DATATRACK_ID:150 TEXT OUTPUT STUDY_ID:ST000099 ANALYSIS_ID:AN000163 PROJECT_ID:PR000089 VERSION 1 CREATED_ON 10-02-2015 #PROJECT PR:PROJECT_TITLE 13C Metabolomics: Applications at Natural Abundance PR:INSTITUTE University of Florida PR:DEPARTMENT Department of Biochemistry and Molecular Biology PR:LABORATORY Edison PR:LAST_NAME Edison PR:FIRST_NAME Arthur PR:ADDRESS R3-226 Academic Research Building, Department of Biochemistry & Molecular PR:ADDRESS Biology, PO Box 100245, Gainesville, FL 32610-0245 PR:EMAIL aedison@ufl.edu PR:PHONE 352-392-4535 PR:PROJECT_SUMMARY - #STUDY ST:STUDY_TITLE NMR analysis of DMD mouse serum ST:STUDY_SUMMARY Measurements were performed on serum using 6 month old C57/B10 control (n=6) and ST:STUDY_SUMMARY mdx (n=8) mice. 1D proton and carbon spectra were collected on these samples ST:INSTITUTE University of Florida ST:DEPARTMENT Department of Biochemistry and Molecular Biology / SECIM ST:LABORATORY Arthur Edison ST:LAST_NAME Edison ST:FIRST_NAME Arthur ST:ADDRESS R3-226 Academic Research Building, Department of Biochemistry & Molecular ST:ADDRESS Biology, PO Box 100245, Gainesville, FL 32610-0245 ST:EMAIL aedison@ufl.edu ST:NUM_GROUPS 2 ST:TOTAL_SUBJECTS 14 ST:PHONE - #SUBJECT SU:SUBJECT_TYPE animal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN C57/BL/10 SU:AGE_OR_AGE_RANGE 6 month old SU:GENDER males SU:SPECIES_GROUP Mammal #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS Mouse Serum 295302_m1 Strain:C57/BL/10ScSn-Dmdmdx/J | model:mdx SUBJECT_SAMPLE_FACTORS Mouse Serum 295302_m2 Strain:C57/BL/10ScSn-Dmdmdx/J | model:mdx SUBJECT_SAMPLE_FACTORS Mouse Serum 295302_m3 Strain:C57/BL/10ScSn-Dmdmdx/J | model:mdx SUBJECT_SAMPLE_FACTORS Mouse Serum 295302_m4 Strain:C57/BL/10ScSn-Dmdmdx/J | model:mdx SUBJECT_SAMPLE_FACTORS Mouse Serum 295303_m2 Strain:C57/BL/10ScSn-Dmdmdx/J | model:mdx SUBJECT_SAMPLE_FACTORS Mouse Serum 295303_m3 Strain:C57/BL/10ScSn-Dmdmdx/J | model:mdx SUBJECT_SAMPLE_FACTORS Mouse Serum 295303_m4 Strain:C57/BL/10ScSn-Dmdmdx/J | model:mdx SUBJECT_SAMPLE_FACTORS Mouse Serum 295303_m5 Strain:C57/BL/10ScSn-Dmdmdx/J | model:mdx SUBJECT_SAMPLE_FACTORS Mouse Serum 298004_m1 Strain:C57/BL/10SnJ | model:Control SUBJECT_SAMPLE_FACTORS Mouse Serum 298004_m2 Strain:C57/BL/10SnJ | model:Control SUBJECT_SAMPLE_FACTORS Mouse Serum 298004_m3 Strain:C57/BL/10SnJ | model:Control SUBJECT_SAMPLE_FACTORS Mouse Serum 298005_m2 Strain:C57/BL/10SnJ | model:Control SUBJECT_SAMPLE_FACTORS Mouse Serum 298005_m3 Strain:C57/BL/10SnJ | model:Control SUBJECT_SAMPLE_FACTORS Mouse Serum 298005_m4 Strain:C57/BL/10SnJ | model:Control #COLLECTION CO:COLLECTION_SUMMARY Mouse serum measurements were performed on 6 replicates of 6 month old C57/B10 CO:COLLECTION_SUMMARY control and 8 replicates of mdx mice. Two hundred ?L of blood was collected by CO:COLLECTION_SUMMARY submandibular puncture, clotted and centrifuged to extract serum. The mice were CO:COLLECTION_SUMMARY then humanely euthanized under 2% isofluorene anesthesia. Serum was flash CO:COLLECTION_SUMMARY frozen in liquid nitrogen and stored at -80 °C. CO:SAMPLE_TYPE BLOOD CO:COLLECTION_METHOD submandibular puncture, CO:COLLECTION_LOCATION University of Florida CO:COLLECTION_FREQUENCY 1 CO:VOLUMEORAMOUNT_COLLECTED 200 microliters CO:STORAGE_CONDITIONS -80 degree celsius CO:BLOOD_SERUM_OR_PLASMA BLOOD Serum #TREATMENT TR:ANIMAL_ENDP_EUTHANASIA 2% Isofluorene TR:TREATMENT_SUMMARY - #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Approximately 100 ?L of serum was lyophilized and re-suspended in 40 ?L 99% D2O SP:SAMPLEPREP_SUMMARY and transferred into 1.5 mm NMR tubes (Norell). SP:PROCESSING_METHOD lyophilized SP:PROCESSING_STORAGE_CONDITIONS -80 degree celsius SP:EXTRACTION_METHOD submandibular puncture #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE - CH:INSTRUMENT_NAME - CH:COLUMN_NAME - #ANALYSIS AN:LABORATORY_NAME Edison AN:ANALYSIS_TYPE NMR AN:SOFTWARE_VERSION VNMRJ AN:OPERATOR_NAME Brittany Lee McMullen AN:DATA_FORMAT Varian #NMR NM:INSTRUMENT_TYPE Varian NM:NMR_EXPERIMENT_TYPE 1D 1H NM:SPECTROMETER_FREQUENCY 600 MHz NM:NMR_PROBE 1.5 mm HTS cryoprobe NM:NMR_SOLVENT D2O NM:NMR_TUBE_SIZE 1.5 mm NM:SHIMMING_METHOD Manual NM:PULSE_SEQUENCE 1D 1H CPMG NM:WATER_SUPPRESSION Presaturation NM:PULSE_WIDTH 45 NM:RECEIVER_GAIN 28 NM:OFFSET_FREQUENCY 4.77 NM:CHEMICAL_SHIFT_REF_CPD TSP NM:TEMPERATURE 22 degree Celsius NM:NUMBER_OF_SCANS 256 NM:DUMMY_SCANS 8 NM:ACQUISITION_TIME 2.0s NM:RELAXATION_DELAY 2s NM:SPECTRAL_WIDTH 16 ppm NM:NUM_DATA_POINTS_ACQUIRED 38422 NM:REAL_DATA_POINTS 19211 NM:ZERO_FILLING 2x NM:APODIZATION Cosine squared NM:CHEMICAL_SHIFT_REF_STD TSP NM:INSTRUMENT_NAME Varian #NMR_BINNED_DATA NMR_BINNED_DATA:UNITS Peak area NMR_BINNED_DATA_START Bin range(ppm) 295302_m1 295302_m2 295302_m3 295302_m4 295303_m2 295303_m3 295303_m4 295303_m5 298004_m1 298004_m2 298004_m3 298005_m2 298005_m3 298005_m4 NMR_BINNED_DATA_END #END