{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000223","ANALYSIS_ID":"AN000332","VERSION":"1","CREATED_ON":"10-02-2015"},

"PROJECT":{"PROJECT_TITLE":"Metabolic Aberrations in Barth Syndrome","PROJECT_TYPE":"Metabolomics","PROJECT_SUMMARY":"The overall objective of thispilot research project is to investigate metabolic mechanisms involved in disturbed intermediary metabolism in Barth Syndrome. Barth Syndrome (BTHS) is x-linked disorder characterized mainly by dilated cardiomyopathy, skeletal muscle weakness and neutropenia.  BTHS is caused by defects in Tafazzin, an enzyme responsible for modifying the acyl chain moieties of cardiolipin, a critical phospholipid of the mitochondrial inner membrane. While a few comprehensive clinical studies of BTHS have been published detailing its cardiac and hematologic features, descriptions of its biochemical characteristics are limited.","INSTITUTE":"Johns Hopkins University","DEPARTMENT":"Department of Pediatrics","LAST_NAME":"Vernon","FIRST_NAME":"Hilary","ADDRESS":"733 N. Broadway St., Baltimore, MD 21205","EMAIL":"hvernon1@jhmi.edu","PHONE":"(443) 923-2783"},

"STUDY":{"STUDY_TITLE":"Metabolic Aberrations in Barth Syndrome","STUDY_TYPE":"Metabolomic analysis of plasma samples","STUDY_SUMMARY":"1) Characterize plasma metabolome in Barth Syndrome 2) To implement targeted, quantitative studies on prospective biomarkers and metabolites of interest derived from the non-targeted phase.","INSTITUTE":"RTI International","DEPARTMENT":"Discovery Science Technology","LABORATORY":"NIH Eastern Regional Comprehensive Metabolomics Resource Core (RTI RCMRC)","LAST_NAME":"Sumner","FIRST_NAME":"Susan","ADDRESS":"3040 E. Cornwallis Road, Research Triangle Park, NC 27709, USA","EMAIL":"ssumner@rti.org","PHONE":"919-541-7479","NUM_GROUPS":"2","TOTAL_SUBJECTS":"37"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"SVM0109",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0205",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0213",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0310",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0312",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0315",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0319",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0321",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0322",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0323",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0402",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0406",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0408",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0416",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0420",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0501",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0503",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0504",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0507",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0511",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0514",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0517",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVM0518",
"Factors":{"Type":"Case"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0221",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0301",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0305",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0307",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0308",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0313",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0315",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0403",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0416",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0417",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0418",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0419",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0511",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVCM0520",
"Factors":{"Type":"Control"}
},
{
"Subject ID":"-",
"Sample ID":"SVPool_1",
"Factors":{"Type":"Pool"}
},
{
"Subject ID":"-",
"Sample ID":"SVPool_2",
"Factors":{"Type":"Pool"}
},
{
"Subject ID":"-",
"Sample ID":"SVPool_3",
"Factors":{"Type":"Pool"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"All participants have been carefully phenotyped and have a molecular confirmation of BTHS.  In order to mitigate the potential effects of intercurrent illness in individual patients, plasma samples were collected under controlled conditions, while the patient is in a homeostatic state of wellness (outpatient setting).  We controlled for nutritional effects by selecting samples hta thave amino acid profiles consistent with 4-6 hours of fasting.  All samples were collected in an EDTA treated collection tube, plasma was separated via centrifugation and has been stored in a controlled setting at -80 degrees Celsius since collection."},

"TREATMENT":{"TREATMENT_SUMMARY":"none"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Aliquots of plasma samples (125 µL) were transferred into pre-labeled 2.0 mL low-bind Eppendorf tubes for experimental samples.  For all samples 125 µL of 0.9% Saline in D2O (containing 2 mM Formate (chemical shift indicator) and 0.2% NaN3 (to prevent bacterial growth)) was added to each tube and were vortexed for 2 min at 5000 rpm.  The samples were then centrifuged at 16,000 rcf for 2 min. Total pools of the samples were prepared (by using 12 µL of each study sample) to serve as QC samples, and 125 µL  aliquots of QC samples were processed in the same way as described above for study samples.  A 200 µL aliquot of the samples were then transferred into 3 mm NMR tubes (Bruker-Biospin, Switzerland), which were kept in a cooler with freeze packs until data acquisition.","PROCESSING_STORAGE_CONDITIONS":"4°C","SAMPLE_RESUSPENSION":"0.9% Saline in D2O","SAMPLE_SPIKING":"Formate"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"-","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},

"ANALYSIS":{"LABORATORY_NAME":"DHMRI","ANALYSIS_TYPE":"NMR","SOFTWARE_VERSION":"Top Spin 3.2","OPERATOR_NAME":"Keven Knagge, Wimal Pathmasiri","DETECTOR_TYPE":"NMR","DATA_FORMAT":"Bruker FID"},

"NM":{"INSTRUMENT_TYPE":"FT NMR","NMR_EXPERIMENT_TYPE":"1D 1H","PULSE_SEQUENCE":"Yes","PULSE_WIDTH":"41967","POWER_LEVEL":"Deuterium","RECEIVER_GAIN":"1 mM (Formate)","OFFSET_FREQUENCY":"950 MHz","PRESATURATION_POWER_LEVEL":"5mm Cryogenically Cooled  ATMA","CHEMICAL_SHIFT_REF_CPD":"D20","TEMPERATURE":"5mm","NUMBER_OF_SCANS":"Topshim (Gradient)","DUMMY_SCANS":"cpmgpr1d","ACQUISITION_TIME":"yes","RELAXATION_DELAY":"8.11","SPECTRAL_WIDTH":"12.589 W","NUM_DATA_POINTS_ACQUIRED":"4","REAL_DATA_POINTS":"4468.1 Hz","ZERO_FILLING":"Formate","APODIZATION":"298 K","BASELINE_CORRECTION_METHOD":"128","CHEMICAL_SHIFT_REF_STD":"16","INSTRUMENT_NAME":"Bruker Avance III","SPECTROMETER_FREQUENCY":"-"},

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