#METABOLOMICS WORKBENCH hormel101_20161014_132832_mwtab.txt DATATRACK_ID:760 VERSION 1 CREATED_ON November 16, 2016, 2:53 pm #PROJECT PR:PROJECT_TITLE Mayo Metabolomics Pilot and Feasibility Award: Mechanisms of sleep apnea and PR:PROJECT_TITLE cardiovascular disease PR:PROJECT_TYPE MS targeted analysis PR:PROJECT_SUMMARY Targeted Metabolic and Biological Markers of Sleepiness Symptoms in Sleep apnea PR:PROJECT_SUMMARY and cardiovascular disease PR:INSTITUTE Mayo Clinic PR:LABORATORY Mayo Clinic Metabolomics Resource Core PR:LAST_NAME Pak PR:FIRST_NAME Victoria PR:ADDRESS unknown PR:EMAIL victoria.pak@yale.edu PR:PHONE 203-737-5065 #STUDY ST:STUDY_TITLE Sleep apnea and cardiovascular samples SCFA, short chain fatty acids panel ST:STUDY_SUMMARY Sleep apnea and cardiovascular samples SCFA, short chain fatty acids panel ST:INSTITUTE Mayo Clinic ST:LABORATORY Mayo Clinic Metabolomics Resource Core ST:LAST_NAME Pak ST:FIRST_NAME Victoria ST:ADDRESS unknown ST:EMAIL victoria.pak@yale.edu ST:PHONE 203-737-5065 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS s_01 ms5688-1 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_02 ms5688-2 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_03 ms5688-3 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_04 ms5688-4 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_05 ms5688-5 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_06 ms5688-6 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_07 ms5688-7 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_08 ms5688-8 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_09 ms5688-9 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_10 ms5688-10 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_11 ms5688-11 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_12 ms5688-12 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_13 ms5688-13 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_14 ms5688-14 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_15 ms5688-15 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_16 ms5688-16 Disease:cardiovascular disease sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_17 ms5688-17 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_18 ms5688-18 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_19 ms5688-19 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_20 ms5688-20 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_21 ms5688-21 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_22 ms5688-22 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_23 ms5688-23 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_24 ms5688-24 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_25 ms5688-25 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_26 ms5688-26 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_27 ms5688-27 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_28 ms5688-28 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_29 ms5688-29 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_30 ms5688-30 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_31 ms5688-31 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_32 ms5688-32 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_33 ms5688-33 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_34 ms5688-34 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_35 ms5688-35 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma SUBJECT_SAMPLE_FACTORS s_36 ms5688-36 Disease:sleep apnea sample type=plasma; Assay=SCFA concentration in Plasma #COLLECTION CO:COLLECTION_SUMMARY For the metabolic markers, 9 ml blood will be drawn into 3 EDTA tubes (3 ml CO:COLLECTION_SUMMARY each) and gently centrifuged at 4°C for 10 min; plasma aliquots were CO:COLLECTION_SUMMARY immediately frozen and stored at -80 °C; will be stored at -80ºC in the CTRC CO:COLLECTION_SUMMARY until all samples are collected and then sent to Mayo Clinic Metabolomics Core. #TREATMENT TR:TREATMENT_SUMMARY Recruitment of subjects will take place from the clinic population in TR:TREATMENT_SUMMARY collaboration with the Penn Sleep Center. Patients newly diagnosed with OSA will TR:TREATMENT_SUMMARY be solicited for via an overnight sleep study, which is general monitoring of TR:TREATMENT_SUMMARY sleep and variety of body functions during sleep. This includes brain (EEG), eye TR:TREATMENT_SUMMARY (EOG), and muscle activity and heart rhythm. The diagnosis of OSA will be TR:TREATMENT_SUMMARY established by full sleep study. First, the PI will screen for eligibility and TR:TREATMENT_SUMMARY obtain informed consent. We will assess height, weight, medical history, TR:TREATMENT_SUMMARY demographics. EDS will be measured subjectively on the night of enrollment in TR:TREATMENT_SUMMARY the study and determined using the Epworth Sleepiness Scale. The information TR:TREATMENT_SUMMARY regarding gender, BMI, smoking, age, medications (name/duration/frequency), and TR:TREATMENT_SUMMARY presence of comorbidities (e.g. hypertension, CVD defined by doctor diagnosis of TR:TREATMENT_SUMMARY myocardial infarction, stroke, or heart failure) will then be obtained via the TR:TREATMENT_SUMMARY electronic medical record. We will also ask subjects whether they exercise by TR:TREATMENT_SUMMARY asking participants the yes/no question: “During the past month, did you TR:TREATMENT_SUMMARY participate in any physical activities or exercises?” Second, the subject will TR:TREATMENT_SUMMARY undergo diagnostic PSG and we will enforce fasting prior to bedtime. Third, TR:TREATMENT_SUMMARY biological samples will be collected in the morning after the sleep study. TR:TREATMENT_SUMMARY Patients who have agreed to participate will undergo a blood draw (20 ml) and TR:TREATMENT_SUMMARY urine collection (30 ml) on the morning after PSG. Laboratory analysis of the TR:TREATMENT_SUMMARY biological samples will be conducted in collaboration with the Clinical TR:TREATMENT_SUMMARY Translation Research Center (CTRC). Finally, subjects will have 1 or 2 practice TR:TREATMENT_SUMMARY sessions with the PVT prior to administration of the task. PVT test will be TR:TREATMENT_SUMMARY conducted 3 times, at approximately 10:00, 12:00, and 14:00 and automatically TR:TREATMENT_SUMMARY scored. This will be done on the day after the sleep study. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY short chain fatty acids panel #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE GC CH:INSTRUMENT_NAME Agilent 7890A CH:COLUMN_NAME Agilent HP5-MS (30m × 0.25mm, 0.25 um) #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:MS_COMMENTS - MS:INSTRUMENT_NAME Agilent 5975C MS:INSTRUMENT_TYPE Single quadrupole MS:MS_TYPE EI MS:ION_MODE POSITIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS micromolar MS_METABOLITE_DATA_START Samples ms5688-1 ms5688-2 ms5688-3 ms5688-4 ms5688-5 ms5688-6 ms5688-7 ms5688-8 ms5688-9 ms5688-10 ms5688-11 ms5688-12 ms5688-13 ms5688-14 ms5688-15 ms5688-16 ms5688-17 ms5688-18 ms5688-19 ms5688-20 ms5688-21 ms5688-22 ms5688-23 ms5688-24 ms5688-25 ms5688-26 ms5688-27 ms5688-28 ms5688-29 ms5688-30 ms5688-31 ms5688-32 ms5688-33 ms5688-34 ms5688-35 ms5688-36 Factors Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:cardiovascular disease Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Disease:sleep apnea Isobutyric 0.365862155 0.208511122 0.345141392 0.078602159 0.198082014 BLOD 0.063436757 BLOD 0.385057166 0.079245613 0.254813967 0.174575361 BLOD BLOD BLOD BLOD BLOD 0.031400592 0.176292779 0.093044447 0.431747413 0.380250688 2.370814837 1.379741793 0.578062711 2.635582958 0.788406812 1.760821075 1.857836324 2.30446297 1.695324133 1.53306569 2.605773904 2.024504298 2.282408333 2.932775201 Butyric 0.174969069 0.057786474 0.108208814 0.105184461 0.048230817 0.120127968 0.118256463 BLOD 0.343967257 0.403352445 0.060039376 0.136861288 0.360698538 0.064419841 0.096295939 BLOD 0.182324649 0.254428539 0.502649714 0.134843314 0.537662896 0.170836206 0.682477041 0.526114366 0.491429163 0.745081534 0.577252545 0.648399682 0.887862235 1.051188047 0.779081529 0.973765715 1.041782257 1.230273156 1.126740176 0.995489665 Isovaleric 0.123985797 0.246835912 0.712153657 0.247940352 0.105901146 0.286654604 0.563625743 0.2216392 0.137853293 0.502542639 0.108470806 0.369012414 0.221526581 0.224648756 0.129350175 0.849318739 0.216676549 0.474007923 0.449187905 1.053888139 0.567054957 0.421298483 0.281758475 0.599816707 0.47399933 0.295951179 0.336126367 0.373468409 0.224816756 0.292445006 0.20857054 0.185195744 0.321739509 0.561058179 0.187704006 0.577494743 Valeric 0.005923708 BLOD 0.027223167 BLOD BLOD BLOD 0.105109336 BLOD 0.005494259 0.330841922 BLOD 0.043915661 0.047823409 BLOD BLOD BLOD 0.096210651 0.026977252 0.009850667 0.045596009 0.180696884 0.07014378 0.034992934 0.076751708 0.041409203 0.080126151 0.062319521 0.196460269 0.143604425 0.115494931 0.068828066 0.251444626 0.168072818 0.112669072 0.106869086 0.02533723 Isocaproic 0.067319607 0.050396816 0.037988526 0.034756824 0.114859689 0.021150617 0.057922347 0.127531739 0.132708648 0.091043371 0.029010406 0.042164487 0.01956993 0.048370333 0.056443783 0.041332617 0.023795153 0.066798679 0.026720291 0.137606696 0.133180749 0.038279685 0.058915607 0.051579166 0.077357218 0.229558221 0.156768306 0.111318543 0.052785173 0.086083829 0.214289249 0.113326102 0.132433018 0.068053636 0.119334383 0.222959398 Hexanoic 0.232340785 0.031020554 0.143441254 0.130288508 0.426082297 0.01043755 0.150520373 0.189572614 0.122464455 0.448398358 0.210521898 0.187659318 0.097363347 BLOD 0.054764401 BLOD 1.026239567 0.172284268 0.094510364 0.324033389 0.650887998 0.388561113 0.409446577 1.806398431 0.193829791 0.281134759 0.382158477 0.893616996 0.713038131 0.806402037 0.479465056 0.282703096 0.746981902 0.399286906 0.972789322 0.404416057 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Isobutyric Butyric Isovaleric Valeric Isocaproic Hexanoic METABOLITES_END #END