{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001156","ANALYSIS_ID":"AN001913","VERSION":"1","CREATED_ON":"March 22, 2019, 11:55 am"},

"PROJECT":{"PROJECT_TITLE":"The Effect of Silicon on Salinity Tolerance and the Associated Metabolomics Profile Changes in Date Palm","PROJECT_SUMMARY":"Since metabolites are the net products of the central dogma of cellular biology, this study was aimed to decipher salinity tolerance depends on the information encoded by the metabolomic profiles of the salt tolerant ‘Umsila’ and susceptible ‘Zabad’ cultivars when grown under salinity. Changes in the metabolomic profiles of the leaf and root tissues were determined using hydrophilic interaction liquid chromatography (HILIC) and reverse phase liquid (RPLC) mass spectrometery.","INSTITUTE":"Sultan Qaboos University, College of Science","DEPARTMENT":"Biology","LABORATORY":"Plant Molecular Biology","LAST_NAME":"Yaish","FIRST_NAME":"Mahmoud","ADDRESS":"Department of Biology, College of Science, Sultan Qaboos University,","EMAIL":"mack.yaish@gmail.com","PHONE":"96824146823"},

"STUDY":{"STUDY_TITLE":"Comparative Metabolomic Profiling of Two Contrasting Date Palm Genotypes under Salinity Stress (part-II)","STUDY_SUMMARY":"Since metabolites are the net products of the central dogma of cellular biology, this study was aimed to decipher salinity tolerance depends on the information encoded by the metabolomic profiles of the salt tolerant ‘Umsila’ and susceptible ‘Zabad’ cultivars when grown under salinity. Changes in the metabolomic profiles of the leaf and root tissues were determined using hydrophilic interaction liquid chromatography (HILIC) and reverse phase liquid (RPLC) mass spectrometry.","INSTITUTE":"Sultan Qaboos University","LAST_NAME":"Yaish","FIRST_NAME":"Mahmoud","ADDRESS":"Department of Biology, College of Science, Sultan Qaboos University","EMAIL":"mack.yaish@gmail.com","PHONE":"968 24146823"},

"SUBJECT":{"SUBJECT_TYPE":"Plant","SUBJECT_SPECIES":"Phoenix dactylifera","TAXONOMY_ID":"42345"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"Control",
"Sample ID":"MCLU-1",
"Factors":{"Factor":"control leaf Umsila"}
},
{
"Subject ID":"Control",
"Sample ID":"MCLU-2",
"Factors":{"Factor":"control leaf Umsila"}
},
{
"Subject ID":"Control",
"Sample ID":"MCLU-3",
"Factors":{"Factor":"control leaf Umsila"}
},
{
"Subject ID":"Control",
"Sample ID":"MCLZ-1",
"Factors":{"Factor":"control leaf Zabad"}
},
{
"Subject ID":"Control",
"Sample ID":"MCLZ-2",
"Factors":{"Factor":"control leaf Zabad"}
},
{
"Subject ID":"Control",
"Sample ID":"MCLZ-3",
"Factors":{"Factor":"control leaf Zabad"}
},
{
"Subject ID":"Control",
"Sample ID":"MCRU-1",
"Factors":{"Factor":"Control root Umsila"}
},
{
"Subject ID":"Control",
"Sample ID":"MCRU-2",
"Factors":{"Factor":"Control root Umsila"}
},
{
"Subject ID":"Control",
"Sample ID":"MCRU-3",
"Factors":{"Factor":"Control root Umsila"}
},
{
"Subject ID":"Control",
"Sample ID":"MCRZ-1",
"Factors":{"Factor":"Control root Zabad"}
},
{
"Subject ID":"Control",
"Sample ID":"MCRZ-2",
"Factors":{"Factor":"Control root Zabad"}
},
{
"Subject ID":"Control",
"Sample ID":"MCRZ-3",
"Factors":{"Factor":"Control root Zabad"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTLU-1",
"Factors":{"Factor":"Treated leaf Umsila"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTLU-2",
"Factors":{"Factor":"Treated leaf Umsila"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTLU-3",
"Factors":{"Factor":"Treated leaf Umsila"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTLZ-1",
"Factors":{"Factor":"Treated leaf Zabad"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTLZ-2",
"Factors":{"Factor":"Treated leaf Zabad"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTLZ-3",
"Factors":{"Factor":"Treated leaf Zabad"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTRU-1",
"Factors":{"Factor":"Treated root Umsila"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTRU-2",
"Factors":{"Factor":"Treated root Umsila"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTRU-3",
"Factors":{"Factor":"Treated root Umsila"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTRZ-1",
"Factors":{"Factor":"Treated root Zabad"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTRZ-2",
"Factors":{"Factor":"Treated root Zabad"}
},
{
"Subject ID":"Salinity",
"Sample ID":"MTRZ-3",
"Factors":{"Factor":"Treated root Zabad"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Leaf and root tissues of Umsila and Zabad cultivars were collected after irrigation either with water or NaCl solution.","SAMPLE_TYPE":"Plant"},

"TREATMENT":{"TREATMENT_SUMMARY":"Seedlings were either irrigated with water or NaCl solution for about 2 months."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Metabolomics and basic data analysis were carried out for service charges at proteomics and mass spectrometry facility, Donald Danforth Plant Science Centre, Sant Louis, USA, using liquid chromatography mass spectrometry. Briefly, a total of 24 samples were analyzed for polar metabolites using hydrophilic interaction liquid chromatography (HILIC) and nonpolar metabolites using reverse phase liquid chromatography (RPLC). The Samples were extracted in 80% methanol at a tissue concentration of 100 mg/mL by a vortex for 10 minutes. The solids were collected by centrifugation, and then the supernatant was filtered through a 0.8 μm spin filter. The extracts were injected into the LC-MS and the data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Q-Exactive","COLUMN_NAME":"chromatography on a 0.5 x 150 mm"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Donald Danforth Plant Science Center, Saint Louis, MO 63132, USA","OPERATOR_NAME":"Bradley S. Evans"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"The ion mode is polarity-switching, which means it scans in positive and negative ion mode during the sample run, switching between the two modes about every 20 milliseconds.","MS_RESULTS_FILE":"ST001156_AN001913_Results.txt UNITS:log10(peak area) Has m/z:No Has RT:Yes RT units:Minutes"}

}