#METABOLOMICS WORKBENCH yli_20190222_112137 DATATRACK_ID:1639 STUDY_ID:ST001171 ANALYSIS_ID:AN001936 PROJECT_ID:PR000783 VERSION 1 CREATED_ON April 17, 2019, 2:30 pm #PROJECT PR:PROJECT_TITLE Metabolomics of World Trade Center Exposed New York City Firefighters PR:PROJECT_TYPE C18 Reversed-Phase Broad Spectrum Metabolomics PR:PROJECT_SUMMARY Particulate matter (PM) exposure and metabolic syndrome (MetSyn) coexist in both PR:PROJECT_SUMMARY industrialized and developing nations. PM and MetSyn are strong risk factors for PR:PROJECT_SUMMARY chronic obstructive pulmonary disease (COPD) and asthma. After the World Trade PR:PROJECT_SUMMARY Center collapse in 9/11/2001, PM-exposed individuals from the Fire Department of PR:PROJECT_SUMMARY New York City (FDNY) developed a progressively lung disease. This nested PR:PROJECT_SUMMARY case-cohort study is composed of never smoking, WTC exposed firefighters with PR:PROJECT_SUMMARY normal pre-9/11 lung function presenting for subspecialty pulmonary evaluation PR:PROJECT_SUMMARY (SPE) before March 2008. Representative cohort controls with serum drawn within PR:PROJECT_SUMMARY six months of 9/11 (n=100). FEV1 at subspecialty exam defined cases: susceptible PR:PROJECT_SUMMARY World Trade Center Lung Injury (WTC-LI) cases (n=50) had FEV1< lower limit of PR:PROJECT_SUMMARY normal (LLN) and resistant WTC-LI cases with FEV1 ≥107% predicted (n=50). This PR:PROJECT_SUMMARY study will determine the metabolomics profile that differentiates firefighters PR:PROJECT_SUMMARY with WTC-LI, firefighters resistant to WTC-LI, and similarly exposed cohort PR:PROJECT_SUMMARY controls. PR:INSTITUTE University of North Carolina PR:DEPARTMENT Nutrition Research Institute PR:LAST_NAME Sumner PR:FIRST_NAME Susan PR:ADDRESS Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition PR:ADDRESS Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 PR:EMAIL susan_sumner @unc.edu PR:PHONE 704-250-5066 #STUDY ST:STUDY_TITLE Metabolomics of World Trade Center Exposed New York City Firefighters ST:STUDY_TYPE C18 Reversed-Phase Broad Spectrum Metabolomics ST:STUDY_SUMMARY Particulate matter (PM) exposure and metabolic syndrome (MetSyn) coexist in both ST:STUDY_SUMMARY industrialized and developing nations. PM and MetSyn are strong risk factors for ST:STUDY_SUMMARY chronic obstructive pulmonary disease (COPD) and asthma. After the World Trade ST:STUDY_SUMMARY Center collapse in 9/11/2001, PM-exposed individuals from the Fire Department of ST:STUDY_SUMMARY New York City (FDNY) developed a progressively lung disease. This nested ST:STUDY_SUMMARY case-cohort study is composed of never smoking, WTC exposed firefighters with ST:STUDY_SUMMARY normal pre-9/11 lung function presenting for subspecialty pulmonary evaluation ST:STUDY_SUMMARY (SPE) before March 2008. Representative cohort controls with serum drawn within ST:STUDY_SUMMARY six months of 9/11 (n=100). FEV1 at subspecialty exam defined cases: susceptible ST:STUDY_SUMMARY World Trade Center Lung Injury (WTC-LI) cases (n=50) had FEV1< lower limit of ST:STUDY_SUMMARY normal (LLN) and resistant WTC-LI cases with FEV1 ≥107% predicted (n=50). This ST:STUDY_SUMMARY study will determine the metabolomics profile that differentiates firefighters ST:STUDY_SUMMARY with WTC-LI, firefighters resistant to WTC-LI, and similarly exposed cohort ST:STUDY_SUMMARY controls. ST:INSTITUTE New York University ST:DEPARTMENT School of Medicine ST:LABORATORY Laboratory at NYU/Bellevue ST:LAST_NAME Nolan ST:FIRST_NAME Anna ST:ADDRESS Academic office 462 1st Avenue, New Bellevue 16, S 16(Office)/ N 20 (Lab) New ST:ADDRESS York, NY 10016 ST:EMAIL anna.nolan@nyulangone.org ST:PHONE 212-263-7283 ST:NUM_GROUPS 3 ST:TOTAL_SUBJECTS 224, 200 study samples, 24 pools ST:NUM_MALES 200 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:AGE_OR_AGE_RANGE 30-60 SU:GENDER Male #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - S_1 Age Group:[50-60) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_10 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_100 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_101 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_102 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_103 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_104 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_105 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_106 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_107 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_108 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_109 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_11 Age Group:[20,30) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_110 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_111 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_112 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_113 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_114 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_115 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_116 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_117 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_118 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_119 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_12 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_120 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_121 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_122 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_123 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_124 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_125 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_126 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_127 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_128 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_129 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_13 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_130 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_131 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_132 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_133 Age Group:[50-60) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_134 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_135 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_136 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_137 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_138 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_139 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_14 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_140 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_141 Age Group:[50-60) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_142 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_143 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_144 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_145 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_146 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_147 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_148 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_149 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_15 Age Group:[50-60) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_150 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_151 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_152 Age Group:[50-60) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_153 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_154 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_155 Age Group:[50-60) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_156 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_157 Age Group:[50-60) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_158 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_159 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_16 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_160 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_161 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_162 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_163 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_164 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_165 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_166 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_167 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_168 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_169 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_17 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_170 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_171 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_172 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_173 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_174 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_175 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_176 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_177 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_178 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_179 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_18 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_180 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_181 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_182 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_183 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_184 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_185 Age Group:[50-60) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_186 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_187 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_188 Age Group:[50-60) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_189 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_19 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_190 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_191 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_192 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_193 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_194 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_195 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_196 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_197 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_198 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_199 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_2 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_20 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_200 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_21 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_22 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_23 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_24 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_25 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_26 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_27 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_28 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_29 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_3 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_30 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_31 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_32 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_33 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_34 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_35 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_36 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_37 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_38 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_39 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_4 Age Group:[60-70) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_40 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_41 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_42 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_43 Age Group:[50-60) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_44 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_45 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_46 Age Group:[50-60) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_47 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_48 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_49 Age Group:[50-60) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_5 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_50 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_51 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_52 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_53 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_54 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_55 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_56 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_57 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_58 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_59 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_6 Age Group:[50-60) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_60 Age Group:[20,30) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_61 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_62 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_63 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_64 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_65 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_66 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_67 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_68 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_69 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_7 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_70 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_71 Age Group:[50-60) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_72 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_73 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_74 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_75 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_76 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_77 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_78 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_79 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_8 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_80 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_81 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_82 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_83 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_84 Age Group:[60-70) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_85 Age Group:[40-50) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_86 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_87 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_88 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_89 Age Group:[30-40) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_9 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_90 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_91 Age Group:[20,30) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_92 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_93 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_94 Age Group:[20,30) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_95 Age Group:[40-50) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_96 Age Group:[30-40) yrs | Group:Control Sex=Male SUBJECT_SAMPLE_FACTORS - S_97 Age Group:[30-40) yrs | Group:Resistant Sex=Male SUBJECT_SAMPLE_FACTORS - S_98 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - S_99 Age Group:[40-50) yrs | Group:WTC-LI Sex=Male SUBJECT_SAMPLE_FACTORS - SP_1 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_10 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_11 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_12 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_13 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_14 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_15 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_16 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_17 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_18 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_19 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_2 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_20 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_21 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_22 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_23 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_24 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_3 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_4 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_5 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_6 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_7 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_8 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - SP_9 Age Group:Sample pool | Group:Sample pool Sex=Sample pool SUBJECT_SAMPLE_FACTORS - CP_1 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_10 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_11 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_12 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_13 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_14 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_15 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_16 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_17 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_18 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_19 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_2 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_20 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_21 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_22 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_23 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_24 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_3 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_4 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_5 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_6 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_7 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_8 Age Group:Chear pool | Group:Chear pool Sex=Chear pool SUBJECT_SAMPLE_FACTORS - CP_9 Age Group:Chear pool | Group:Chear pool Sex=Chear pool #COLLECTION CO:COLLECTION_SUMMARY Fasting blood drawn CO:SAMPLE_TYPE Blood (serum) CO:COLLECTION_LOCATION Bureau of Health Services (BHS) and the FDNY Headquarters, (9 Metrotech Center, CO:COLLECTION_LOCATION Brooklyn, NY) CO:COLLECTION_DURATION 6 month after 9/11/2001 and once at 2008 CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY No available #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The Fire Department of New York City (FDNY) serum sampling occurred at the SP:SAMPLEPREP_SUMMARY Bureau of Health Services (BHS) and the FDNY Headquarters (9 Metrotech Center, SP:SAMPLEPREP_SUMMARY Brooklyn, NY). Serum processing and analysis occurred in the PI’s Laboratory SP:SAMPLEPREP_SUMMARY at New York University /Bellevue. The specimens were stored in the New York SP:SAMPLEPREP_SUMMARY University-The Fire Department of New York City- World Trade Center Lung Injury SP:SAMPLEPREP_SUMMARY Biorepository (NYU-FDNY WTC) in Dr. Nolan’s laboratory. Each stored specimen SP:SAMPLEPREP_SUMMARY was labeled with a unique code. This unique code on the sample was linked to a SP:SAMPLEPREP_SUMMARY specific subject. Samples were stored in -80 oC and shipped to the Eastern SP:SAMPLEPREP_SUMMARY Regional Comprehensive Metabolomics Resource Core (ERCMRC) on dry ice. Table 1 SP:SAMPLEPREP_SUMMARY lists the sample ID and associated metadata provided. Serum samples were thawed SP:SAMPLEPREP_SUMMARY at 4 °C overnight. All samples were vortexed via multiple tube vortex mixer for SP:SAMPLEPREP_SUMMARY 2 min at 3000 rpm. Serum samples were centrifuged at 1000 rpm for 30 sec. A SP:SAMPLEPREP_SUMMARY volume of 8 µL aliquot of each studied samples was transferred into a 10.0 mL SP:SAMPLEPREP_SUMMARY tube to make the Study Pool (SP). A volume of 1500 µL of CHEAR Reference Plasma SP:SAMPLEPREP_SUMMARY was used for the CHEAR Pool (CP). An aliquot of 50 µL serum (including SP:SAMPLEPREP_SUMMARY experimental samples, SP, and CP) was transferred to a new, pre-labeled Lo-Bind SP:SAMPLEPREP_SUMMARY Eppendorf tube. There were total 200 experimental samples, 24 SP samples, and 24 SP:SAMPLEPREP_SUMMARY CP samples in this study. The samples were extracted by 400 µL methanol with SP:SAMPLEPREP_SUMMARY 500 ng/ml tryptophan-d5 as internal standard via a multiple tube vortex mixer SP:SAMPLEPREP_SUMMARY for 2 min at 5000 rpm. Protein precipitate was pelleted using a centrifuge SP:SAMPLEPREP_SUMMARY operating at room temperature for 4 min at 16,000 rcf. A volume of 350 µl of SP:SAMPLEPREP_SUMMARY the supernatant was transferred into a pre-labeled 2.0 ml Low-bind Eppendorf SP:SAMPLEPREP_SUMMARY tube, and then dried by a SpeedVac overnight. For immediate analysis, 100 µL of SP:SAMPLEPREP_SUMMARY water-methanol solution (95:5, v/v) was added to reconstitute the dried SP:SAMPLEPREP_SUMMARY extracts, and the samples were thoroughly mixed on multiple tube vortex mixer SP:SAMPLEPREP_SUMMARY for 10 min at 5000 rpm. Samples were centrifuged at 4°C for 4 min at 16,000 SP:SAMPLEPREP_SUMMARY rcf. The supernatant was transferred to pre-labeled autosampler vials for data SP:SAMPLEPREP_SUMMARY acquisition by LC-MS. Broad-spectrum metabolomics was conducted using a Thermo SP:SAMPLEPREP_SUMMARY Scientific™ Vanquish™ UPHPLC - Q Exactive™ HF-X Orbitrap System. SP:SAMPLEPREP_SUMMARY Metabolites were separated on an Acquity UPLC HSS T3 C18 (2.1 X 100 mm, 1.8 µm) SP:SAMPLEPREP_SUMMARY operating at 50 C using a reversed phase gradient separation with Water with SP:SAMPLEPREP_SUMMARY 0.1% Formic Acid (v/v) as mobile phase A and Methanol with 0.1% Formic Acid SP:SAMPLEPREP_SUMMARY (v/v) as mobile phase B. A 5 µL was injected into the instrument, and MS was SP:SAMPLEPREP_SUMMARY collected between 50-750 m/z in positive mode with the MS/MS data triggered by SP:SAMPLEPREP_SUMMARY the Data-Dependent Acquisition. SP:EXTRACTION_METHOD Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard SP:EXTRACT_STORAGE -80℃ SP:SAMPLE_RESUSPENSION Water-Methanol (95:5, v/v) SP:SAMPLE_SPIKING Tryptophan-d5 #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Waters Acquity BEH HSS T3 (100 x 2.1mm, 1.8um) CH:FLOW_GRADIENT Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. CH:FLOW_GRADIENT 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 CH:FLOW_RATE 0.4 mL/min CH:COLUMN_TEMPERATURE 50 CH:SOLVENT_A Water with 0.1% Formic Acid CH:SOLVENT_B Methanol with 0.1% Formic Acid CH:INJECTION_TEMPERATURE 8 CH:INTERNAL_STANDARD Tryptophan-d5 #ANALYSIS AN:ANALYSIS_TYPE MS AN:OPERATOR_NAME Hossein Maleki AN:DETECTOR_TYPE Orbitrap AN:SOFTWARE_VERSION Xcarlibur 4.1.31.9 AN:ACQUISITION_DATE 7/20/2018 AN:DATA_FORMAT Profile #MS MS:INSTRUMENT_NAME Thermo Q Exactive HF hybrid Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS LC-MS/MS data was acquired by data dependent acquisition;Progenesis QI was used MS:MS_COMMENTS to process the raw data. MS:ACQUISITION_DATE 7/20/2018 MS:CAPILLARY_TEMPERATURE 275 oC MS:CAPILLARY_VOLTAGE 3.75 kV MS:COLLISION_ENERGY 20, 30, 45 MS:COLLISION_GAS N2 MS:DRY_GAS_FLOW 45 arbitary unit MS:DRY_GAS_TEMP 325 oC MS:FRAGMENTATION_METHOD CID MS:GAS_PRESSURE 45 arbitary unit MS:ION_SOURCE_TEMPERATURE 325 oC MS:ION_SPRAY_VOLTAGE 3.75 kV MS:IONIZATION ESI MS:IONIZATION_POTENTIAL positive MS:MASS_ACCURACY 5ppm MS:SOURCE_TEMPERATURE 325 oC MS:SPRAY_VOLTAGE 3.75 kV MS:RESOLUTION_SETTING 120000 MS:SCAN_RANGE_MOVERZ 70-1050 m/z MS:SCANNING_RANGE 70-1050 m/z MS:MS_RESULTS_FILE ST001171_AN001936_Results.txt UNITS:peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END