#METABOLOMICS WORKBENCH cclish_20201105_092933_mwtab.txt DATATRACK_ID:2230 STUDY_ID:ST001522 ANALYSIS_ID:AN002537 PROJECT_ID:000000 VERSION 1 CREATED_ON November 5, 2020, 2:22 pm #PROJECT PR:PROJECT_TITLE Role of Diet in the Reconstitution of the Human Gut Microbiome and its PR:PROJECT_TITLE Metabolome PR:PROJECT_TYPE Observational study PR:PROJECT_SUMMARY We studied the impact of three divergent diets, vegan, omnivore, and a synthetic PR:PROJECT_SUMMARY enteral nutrition (EEN) diet lacking fiber, on the human gut microbiome and its PR:PROJECT_SUMMARY metabolome in a longitudinal analysis that included a microbiota depletion PR:PROJECT_SUMMARY intervention. Hybrid nontargeted LC-MS methods were used to profile stool and PR:PROJECT_SUMMARY plasma metabolites. PR:INSTITUTE Broad Institute of MIT and Harvard PR:LAST_NAME Clish PR:FIRST_NAME Clary PR:ADDRESS 415 Main Street, Cambridge, MA, 02142, USA PR:EMAIL clary@broadinstitute.org PR:PHONE 617-714-7654 PR:FUNDING_SOURCE Crohn’s & Colitis Foundation, P30 DK 050306, PennCHOP Microbiome Program, and PR:FUNDING_SOURCE the Penn Center for Nutritional Science and Medicine PR:CONTRIBUTORS Ceylan Tanes, Kyle Bittinger, Yuan Gao, Elliot S. Friedman, Lisa Nessel, Unmesha PR:CONTRIBUTORS Roy Paladhi, Lillian Chau, Erika Panfen, Michael A. Fischbach, Jonathan Braun, PR:CONTRIBUTORS Ramnik J. Xavier, Clary B. Clish, Hongzhe Li, Frederic D. Bushman, James D. PR:CONTRIBUTORS Lewis, Gary D. Wu #STUDY ST:STUDY_TITLE Plasma unknowns profiled using hybrid nontargeted methods (part-IV) ST:STUDY_SUMMARY We determined the effect of diet on the composition and metabolic function of ST:STUDY_SUMMARY human gut microbiome using a controlled feeding experiment with three divergent ST:STUDY_SUMMARY diets (vegan, omnivore, and an exclusive enteral nutrition diet (EEN) devoid of ST:STUDY_SUMMARY dietary fiber) in the Food And Resulting Microbial Metabolites (FARMM) study. ST:STUDY_SUMMARY The study included an antibiotic and polyethylene glycol (Abx/PEG) intervention ST:STUDY_SUMMARY to dynamically assess the effect of diet on the reconstitution of the gut ST:STUDY_SUMMARY microbiota and its associated fecal and plasma metabolome. Samples from thirty ST:STUDY_SUMMARY healthy volunteers between the ages of 18 and 60, 10 mper diet group were ST:STUDY_SUMMARY analyzed. Self-reported vegans were required to have followed a vegan diet for a ST:STUDY_SUMMARY minimum of 6 months prior to enrollment. Key exclusion criteria included ST:STUDY_SUMMARY inflammatory bowel disease, celiac disease, or other chronic intestinal ST:STUDY_SUMMARY disorders; prior bowel resection surgery other than appendectomy; baseline bowel ST:STUDY_SUMMARY frequency less than every 2 days or greater than 3 times daily; creatinine ST:STUDY_SUMMARY concentration greater than the upper limit of normal; diabetes mellitus; ST:STUDY_SUMMARY currently smoking; body mass index (BMI) <18.5 or >35; and use of antibiotics ST:STUDY_SUMMARY and probiotics in the prior 6 months. The 10 vegans continued to follow their ST:STUDY_SUMMARY usual diet as outpatients. All participants completed the Diet History ST:STUDY_SUMMARY Questionnaire II (DHQ II), a food frequency questionnaire developed by the Risk ST:STUDY_SUMMARY Factor Monitoring and Methods Branch of the National Cancer Institute. The vegan ST:STUDY_SUMMARY participants also completed three 24 hour diet recalls with a dietitian in the ST:STUDY_SUMMARY week prior to starting antibiotics. We randomly assigned the 20 omnivores to ST:STUDY_SUMMARY receive an omnivore diet or EEN (Modulen® IBD) while residing in an inpatient ST:STUDY_SUMMARY research unit. The macronutrient composition of Modulen® is protein 36g, fat ST:STUDY_SUMMARY 47g, and carbohydrate 110g per 1000 Kcal. The two omnivore diets were engineered ST:STUDY_SUMMARY to have a similar composition to EEN. All the subjects consumed the menu A on ST:STUDY_SUMMARY day 11 and menu B on days 4 and 14. Diets for omnivores were constructed to ST:STUDY_SUMMARY provide the expected total calories required per day for the participant to ST:STUDY_SUMMARY maintain their current weight and were adjusted if there was weight gain or loss ST:STUDY_SUMMARY of more than 2.5 pounds. On days 6, 7, and 8, inpatients participants received ST:STUDY_SUMMARY vancomycin 500mg orally every 6 hours and neomycin 1000mg orally every 6 hours. ST:STUDY_SUMMARY On day 7, participants consumed 4L of polyethylene glycol (PEG) based bowel ST:STUDY_SUMMARY purgative (GoLytely®). Participants in the omnivore and EEN arms left the ST:STUDY_SUMMARY inpatient research unit only under the direct supervision of a research staff ST:STUDY_SUMMARY member. The vegan outpatients reported to the hospital twice on days 6,7 and 8 ST:STUDY_SUMMARY to receive antibiotics and to consume PEG on day 7. The first stool sample of ST:STUDY_SUMMARY each day of the inpatient groups was collected, aliquoted and frozen immediately ST:STUDY_SUMMARY at -80oC. Blood was collected on days 1, 5, 9, 12 and 15 from which plasma ST:STUDY_SUMMARY aliquots were immediately isolated and frozen at -80oC. The outpatient ST:STUDY_SUMMARY participants following the vegan diet also had blood collected on days 1, 5, 9, ST:STUDY_SUMMARY 12, and 15 from which plasma aliquots were immediately isolated and frozen at ST:STUDY_SUMMARY -80oC. Among these participants, the first stool of the day was collected at ST:STUDY_SUMMARY home daily and kept on ice packs until it was brought to the research unit where ST:STUDY_SUMMARY it could be aliquoted and frozen. Samples from vegans were received for ST:STUDY_SUMMARY aliquoting within 24 hours and on average within 4 hours (Wu et al., 2010). Day ST:STUDY_SUMMARY 0 stool was not collected from vegans since their diet did not change. The ST:STUDY_SUMMARY aliquoted amounts for all samples ranged from 500 mg to 1 g. The aliquots were ST:STUDY_SUMMARY taken from different areas of the sample. Remaining sample was then collected ST:STUDY_SUMMARY into one residual 50 ml conical tube with a tongue depressor. Any remaining ST:STUDY_SUMMARY stool was discarded. The University of Pennsylvania Institutional Review Board ST:STUDY_SUMMARY (IRB) approved the research protocol. ST:INSTITUTE Broad Institute of MIT and Harvard ST:LAST_NAME Clish ST:FIRST_NAME Clary ST:ADDRESS 415 Main Street, Cambridge, MA, 02142, USA ST:EMAIL clary@broadinstitute.org ST:PHONE 617-714-7654 ST:NUM_GROUPS 3 ST:TOTAL_SUBJECTS 30 ST:NUM_MALES 20 ST:NUM_FEMALES 10 ST:STUDY_COMMENTS plasma samples collected at baseline and 3-4 timepoints #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:AGE_OR_AGE_RANGE 20-60 SU:WEIGHT_OR_WEIGHT_RANGE BMI range: 20-35 SU:GENDER Male and female SU:HUMAN_RACE White; American Indian/Alaskan Native; Black/African American SU:HUMAN_ETHNICITY Hispanic or Latino; Non-Hispanic or Latino SU:HUMAN_TRIAL_TYPE Intervention trial SU:HUMAN_EXCLUSION_CRITERIA Key exclusion criteria included inflammatory bowel disease, celiac disease, or SU:HUMAN_EXCLUSION_CRITERIA other chronic intestinal disorders; prior bowel resection surgery other than SU:HUMAN_EXCLUSION_CRITERIA appendectomy; baseline bowel frequency less than every 2 days or greater than 3 SU:HUMAN_EXCLUSION_CRITERIA times daily; creatinine concentration greater than the upper limit of normal; SU:HUMAN_EXCLUSION_CRITERIA diabetes mellitus; currently smoking; body mass index (BMI) <18.5 or >35; and SU:HUMAN_EXCLUSION_CRITERIA use of antibiotics and probiotics in the prior 6 months #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS 9002 9002-3-PA Study_Diet:Vegan Type=Plasma; Age=20; Sex=Male; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0078_WUG_FARMM_HIL-9002-3-PA.raw 0078_WUG_FARMM_LIP-9002-3-PA.raw 0078_WU_FARMM_CMH_9002-3-PA.raw 0078_WUG_FARMM_FFA-9002-3-PA.raw SUBJECT_SAMPLE_FACTORS 9002 9002-3-PB Study_Diet:Vegan Type=Plasma; Age=20; Sex=Male; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0076_WUG_FARMM_HIL-9002-3-PB.raw 0076_WUG_FARMM_LIP-9002-3-PB.raw 0076_WU_FARMM_CMH_9002-3-PB.raw 0076_WUG_FARMM_FFA-9002-3-PB.raw SUBJECT_SAMPLE_FACTORS 9002 9002-3-PC Study_Diet:Vegan Type=Plasma; Age=20; Sex=Male; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0079_WUG_FARMM_HIL-9002-3-PC.raw 0079_WUG_FARMM_LIP-9002-3-PC.raw 0079_WU_FARMM_CMH_9002-3-PC.raw 0079_WUG_FARMM_FFA-9002-3-PC.raw SUBJECT_SAMPLE_FACTORS 9002 9002-3-PD Study_Diet:Vegan Type=Plasma; Age=20; Sex=Male; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0080_WUG_FARMM_HIL-9002-3-PD.raw 0080_WUG_FARMM_LIP-9002-3-PD.raw 0080_WU_FARMM_CMH_9002-3-PD.raw 0080_WUG_FARMM_FFA-9002-3-PD.raw SUBJECT_SAMPLE_FACTORS 9002 9002-3-PE Study_Diet:Vegan Type=Plasma; Age=20; Sex=Male; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0077_WUG_FARMM_HIL-9002-3-PE.raw 0077_WUG_FARMM_LIP-9002-3-PE.raw 0077_WU_FARMM_CMH_9002-3-PE.raw 0077_WUG_FARMM_FFA-9002-3-PE.raw SUBJECT_SAMPLE_FACTORS 9003 9003-2-PA Study_Diet:Modulen Type=Plasma; Age=20; Sex=Male; BMI=20.3; Ethnicity=NA; Race=White; Time=Day 15; RAW_FILE_NAME=0144_WUG_FARMM_HIL-9003-2-PA.raw 0144_WUG_FARMM_LIP-9003-2-PA.raw 0144_WU_FARMM_CMH_9003-2-PA.raw 0144_WUG_FARMM_FFA-9003-2-PA.raw SUBJECT_SAMPLE_FACTORS 9003 9003-2-PB Study_Diet:Modulen Type=Plasma; Age=20; Sex=Male; BMI=20.3; Ethnicity=NA; Race=White; Time=Day 12; RAW_FILE_NAME=0141_WUG_FARMM_HIL-9003-2-PB.raw 0141_WUG_FARMM_LIP-9003-2-PB.raw 0141_WU_FARMM_CMH_9003-2-PB.raw 0141_WUG_FARMM_FFA-9003-2-PB.raw SUBJECT_SAMPLE_FACTORS 9003 9003-2-PC Study_Diet:Modulen Type=Plasma; Age=20; Sex=Male; BMI=20.3; Ethnicity=NA; Race=White; Time=Day 9; RAW_FILE_NAME=0143_WUG_FARMM_HIL-9003-2-PC.raw 0143_WUG_FARMM_LIP-9003-2-PC.raw 0143_WU_FARMM_CMH_9003-2-PC.raw 0143_WUG_FARMM_FFA-9003-2-PC.raw SUBJECT_SAMPLE_FACTORS 9003 9003-2-PD Study_Diet:Modulen Type=Plasma; Age=20; Sex=Male; BMI=20.3; Ethnicity=NA; Race=White; Time=Day 5; RAW_FILE_NAME=0142_WUG_FARMM_HIL-9003-2-PD.raw 0142_WUG_FARMM_LIP-9003-2-PD.raw 0142_WU_FARMM_CMH_9003-2-PD.raw 0142_WUG_FARMM_FFA-9003-2-PD.raw SUBJECT_SAMPLE_FACTORS 9003 9003-2-PE Study_Diet:Modulen Type=Plasma; Age=20; Sex=Male; BMI=20.3; Ethnicity=NA; Race=White; Time=Baseline; RAW_FILE_NAME=0145_WUG_FARMM_HIL-9003-2-PE.raw 0145_WUG_FARMM_LIP-9003-2-PE.raw 0145_WU_FARMM_CMH_9003-2-PE.raw 0145_WUG_FARMM_FFA-9003-2-PE.raw SUBJECT_SAMPLE_FACTORS 9004 9004-3-PA Study_Diet:Vegan Type=Plasma; Age=24; Sex=Male; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0091_WUG_FARMM_HIL-9004-3-PA.raw 0091_WUG_FARMM_LIP-9004-3-PA.raw 0091_WU_FARMM_CMH_9004-3-PA.raw 0091_WUG_FARMM_FFA-9004-3-PA.raw SUBJECT_SAMPLE_FACTORS 9004 9004-3-PB Study_Diet:Vegan Type=Plasma; Age=24; Sex=Male; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0092_WUG_FARMM_HIL-9004-3-PB.raw 0092_WUG_FARMM_LIP-9004-3-PB.raw 0092_WU_FARMM_CMH_9004-3-PB.raw 0092_WUG_FARMM_FFA-9004-3-PB.raw SUBJECT_SAMPLE_FACTORS 9004 9004-3-PC Study_Diet:Vegan Type=Plasma; Age=24; Sex=Male; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0095_WUG_FARMM_HIL-9004-3-PC.raw 0095_WUG_FARMM_LIP-9004-3-PC.raw 0095_WU_FARMM_CMH_9004-3-PC.raw 0095_WUG_FARMM_FFA-9004-3-PC.raw SUBJECT_SAMPLE_FACTORS 9004 9004-3-PD Study_Diet:Vegan Type=Plasma; Age=24; Sex=Male; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0093_WUG_FARMM_HIL-9004-3-PD.raw 0093_WUG_FARMM_LIP-9004-3-PD.raw 0093_WU_FARMM_CMH_9004-3-PD.raw 0093_WUG_FARMM_FFA-9004-3-PD.raw SUBJECT_SAMPLE_FACTORS 9004 9004-3-PE Study_Diet:Vegan Type=Plasma; Age=24; Sex=Male; BMI=21.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0094_WUG_FARMM_HIL-9004-3-PE.raw 0094_WUG_FARMM_LIP-9004-3-PE.raw 0094_WU_FARMM_CMH_9004-3-PE.raw 0094_WUG_FARMM_FFA-9004-3-PE.raw SUBJECT_SAMPLE_FACTORS 9005 9005-1-PA Study_Diet:Western Type=Plasma; Age=23; Sex=Male; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0109_WUG_FARMM_HIL-9005-1-PA.raw 0150d_0109ri_WUG_FARMM_LIP-9005-1-PA.raw 0109_WU_FARMM_CMH_9005-1-PA.raw 0109_WUG_FARMM_FFA-9005-1-PA.raw SUBJECT_SAMPLE_FACTORS 9005 9005-1-PB Study_Diet:Western Type=Plasma; Age=23; Sex=Male; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0106_WUG_FARMM_HIL-9005-1-PB.raw 0106_WUG_FARMM_LIP-9005-1-PB.raw 0106_WU_FARMM_CMH_9005-1-PB.raw 0106_WUG_FARMM_FFA-9005-1-PB.raw SUBJECT_SAMPLE_FACTORS 9005 9005-1-PC Study_Diet:Western Type=Plasma; Age=23; Sex=Male; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0108_WUG_FARMM_HIL-9005-1-PC.raw 0150c_0108ri_WUG_FARMM_LIP-9005-1-PC.raw 0108_WU_FARMM_CMH_9005-1-PC.raw 0108_WUG_FARMM_FFA-9005-1-PC.raw SUBJECT_SAMPLE_FACTORS 9005 9005-1-PD Study_Diet:Western Type=Plasma; Age=23; Sex=Male; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0107_WUG_FARMM_HIL-9005-1-PD.raw 0107_WUG_FARMM_LIP-9005-1-PD.raw 0107_WU_FARMM_CMH_9005-1-PD.raw 0107_WUG_FARMM_FFA-9005-1-PD.raw SUBJECT_SAMPLE_FACTORS 9005 9005-1-PE Study_Diet:Western Type=Plasma; Age=23; Sex=Male; BMI=21.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0110_WUG_FARMM_HIL-9005-1-PE.raw 0150e_0110ri_WUG_FARMM_LIP-9005-1-PE.raw 0110_WU_FARMM_CMH_9005-1-PE.raw 0110_WUG_FARMM_FFA-9005-1-PE.raw SUBJECT_SAMPLE_FACTORS 9006 9006-1-PA Study_Diet:Western Type=Plasma; Age=24; Sex=Male; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0047_WUG_FARMM_HIL-9006-1-PA.raw 0047_WUG_FARMM_LIP-9006-1-PA.raw 0047_WU_FARMM_CMH_9006-1-PA.raw 0047_WUG_FARMM_FFA-9006-1-PA.raw SUBJECT_SAMPLE_FACTORS 9006 9006-1-PB Study_Diet:Western Type=Plasma; Age=24; Sex=Male; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0048_WUG_FARMM_HIL-9006-1-PB.raw 0048_WUG_FARMM_LIP-9006-1-PB.raw 0048_WU_FARMM_CMH_9006-1-PB.raw 0048_WUG_FARMM_FFA-9006-1-PB.raw SUBJECT_SAMPLE_FACTORS 9006 9006-1-PC Study_Diet:Western Type=Plasma; Age=24; Sex=Male; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0050_WUG_FARMM_HIL-9006-1-PC.raw 0050_WUG_FARMM_LIP-9006-1-PC.raw 0050_WU_FARMM_CMH_9006-1-PC.raw 0050_WUG_FARMM_FFA-9006-1-PC.raw SUBJECT_SAMPLE_FACTORS 9006 9006-1-PD Study_Diet:Western Type=Plasma; Age=24; Sex=Male; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0046_WUG_FARMM_HIL-9006-1-PD.raw 0046_WUG_FARMM_LIP-9006-1-PD.raw 0046_WU_FARMM_CMH_9006-1-PD.raw 0046_WUG_FARMM_FFA-9006-1-PD.raw SUBJECT_SAMPLE_FACTORS 9006 9006-1-PE Study_Diet:Western Type=Plasma; Age=24; Sex=Male; BMI=28.2; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0049_WUG_FARMM_HIL-9006-1-PE.raw 0049_WUG_FARMM_LIP-9006-1-PE.raw 0049_WU_FARMM_CMH_9006-1-PE.raw 0049_WUG_FARMM_FFA-9006-1-PE.raw SUBJECT_SAMPLE_FACTORS 9008 9008-3-PA Study_Diet:Vegan Type=Plasma; Age=23; Sex=Male; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0103_WUG_FARMM_HIL-9008-3-PA.raw 0103_WUG_FARMM_LIP-9008-3-PA.raw 0103_WU_FARMM_CMH_9008-3-PA.raw 0103_WUG_FARMM_FFA-9008-3-PA.raw SUBJECT_SAMPLE_FACTORS 9008 9008-3-PB Study_Diet:Vegan Type=Plasma; Age=23; Sex=Male; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0105_WUG_FARMM_HIL-9008-3-PB.raw 0105_WUG_FARMM_LIP-9008-3-PB.raw 0105_WU_FARMM_CMH_9008-3-PB.raw 0105_WUG_FARMM_FFA-9008-3-PB.raw SUBJECT_SAMPLE_FACTORS 9008 9008-3-PC Study_Diet:Vegan Type=Plasma; Age=23; Sex=Male; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0101_WUG_FARMM_HIL-9008-3-PC.raw 0101_WUG_FARMM_LIP-9008-3-PC.raw 0101_WU_FARMM_CMH_9008-3-PC.raw 0101_WUG_FARMM_FFA-9008-3-PC.raw SUBJECT_SAMPLE_FACTORS 9008 9008-3-PD Study_Diet:Vegan Type=Plasma; Age=23; Sex=Male; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0104_WUG_FARMM_HIL-9008-3-PD.raw 0104_WUG_FARMM_LIP-9008-3-PD.raw 0104_WU_FARMM_CMH_9008-3-PD.raw 0104_WUG_FARMM_FFA-9008-3-PD.raw SUBJECT_SAMPLE_FACTORS 9008 9008-3-PE Study_Diet:Vegan Type=Plasma; Age=23; Sex=Male; BMI=20; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0102_WUG_FARMM_HIL-9008-3-PE.raw 0102_WUG_FARMM_LIP-9008-3-PE.raw 0102_WU_FARMM_CMH_9008-3-PE.raw 0102_WUG_FARMM_FFA-9008-3-PE.raw SUBJECT_SAMPLE_FACTORS 9009 9009-2-PA Study_Diet:Modulen Type=Plasma; Age=23; Sex=Male; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0131_WUG_FARMM_HIL-9009-2-PA.raw 0131_WUG_FARMM_LIP-9009-2-PA.raw 0131_WU_FARMM_CMH_9009-2-PA.raw 0131_WUG_FARMM_FFA-9009-2-PA.raw SUBJECT_SAMPLE_FACTORS 9009 9009-2-PB Study_Diet:Modulen Type=Plasma; Age=23; Sex=Male; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0132_WUG_FARMM_HIL-9009-2-PB.raw 0132_WUG_FARMM_LIP-9009-2-PB.raw 0132_WU_FARMM_CMH_9009-2-PB.raw 0132_WUG_FARMM_FFA-9009-2-PB.raw SUBJECT_SAMPLE_FACTORS 9009 9009-2-PC Study_Diet:Modulen Type=Plasma; Age=23; Sex=Male; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0133_WUG_FARMM_HIL-9009-2-PC.raw 0133_WUG_FARMM_LIP-9009-2-PC.raw 0133_WU_FARMM_CMH_9009-2-PC.raw 0133_WUG_FARMM_FFA-9009-2-PC.raw SUBJECT_SAMPLE_FACTORS 9009 9009-2-PD Study_Diet:Modulen Type=Plasma; Age=23; Sex=Male; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0134_WUG_FARMM_HIL-9009-2-PD.raw 0134_WUG_FARMM_LIP-9009-2-PD.raw 0134_WU_FARMM_CMH_9009-2-PD.raw 0134_WUG_FARMM_FFA-9009-2-PD.raw SUBJECT_SAMPLE_FACTORS 9009 9009-2-PE Study_Diet:Modulen Type=Plasma; Age=23; Sex=Male; BMI=22.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0135_WUG_FARMM_HIL-9009-2-PE.raw 0135_WUG_FARMM_LIP-9009-2-PE.raw 0135_WU_FARMM_CMH_9009-2-PE.raw 0135_WUG_FARMM_FFA-9009-2-PE.raw SUBJECT_SAMPLE_FACTORS 9010 9010-1-PA Study_Diet:Western Type=Plasma; Age=24; Sex=Female; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0149_WUG_FARMM_HIL-9010-1-PA.raw 0149_WUG_FARMM_LIP-9010-1-PA.raw 0149_WU_FARMM_CMH_9010-1-PA.raw 0149_WUG_FARMM_FFA-9010-1-PA.raw SUBJECT_SAMPLE_FACTORS 9010 9010-1-PB Study_Diet:Western Type=Plasma; Age=24; Sex=Female; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0148_WUG_FARMM_HIL-9010-1-PB.raw 0148_WUG_FARMM_LIP-9010-1-PB.raw 0148_WU_FARMM_CMH_9010-1-PB.raw 0148_WUG_FARMM_FFA-9010-1-PB.raw SUBJECT_SAMPLE_FACTORS 9010 9010-1-PC Study_Diet:Western Type=Plasma; Age=24; Sex=Female; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0147_WUG_FARMM_HIL-9010-1-PC.raw 0147_WUG_FARMM_LIP-9010-1-PC.raw 0147_WU_FARMM_CMH_9010-1-PC.raw 0147_WUG_FARMM_FFA-9010-1-PC.raw SUBJECT_SAMPLE_FACTORS 9010 9010-1-PD Study_Diet:Western Type=Plasma; Age=24; Sex=Female; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0146_WUG_FARMM_HIL-9010-1-PD.raw 0146_WUG_FARMM_LIP-9010-1-PD.raw 0146_WU_FARMM_CMH_9010-1-PD.raw 0146_WUG_FARMM_FFA-9010-1-PD.raw SUBJECT_SAMPLE_FACTORS 9010 9010-1-PE Study_Diet:Western Type=Plasma; Age=24; Sex=Female; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0150_WUG_FARMM_HIL-9010-1-PE.raw 0150_WUG_FARMM_LIP-9010-1-PE.raw 0150_WU_FARMM_CMH_9010-1-PE.raw 0150_WUG_FARMM_FFA-9010-1-PE.raw SUBJECT_SAMPLE_FACTORS 9013 9013-2-PA Study_Diet:Modulen Type=Plasma; Age=40; Sex=Male; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0075_WUG_FARMM_HIL-9013-2-PA.raw 0075_WUG_FARMM_LIP-9013-2-PA.raw 0075_WU_FARMM_CMH_9013-2-PA.raw 0075_WUG_FARMM_FFA-9013-2-PA.raw SUBJECT_SAMPLE_FACTORS 9013 9013-2-PB Study_Diet:Modulen Type=Plasma; Age=40; Sex=Male; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0074_WUG_FARMM_HIL-9013-2-PB.raw 0074_WUG_FARMM_LIP-9013-2-PB.raw 0074_WU_FARMM_CMH_9013-2-PB.raw 0074_WUG_FARMM_FFA-9013-2-PB.raw SUBJECT_SAMPLE_FACTORS 9013 9013-2-PC Study_Diet:Modulen Type=Plasma; Age=40; Sex=Male; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0072_WUG_FARMM_HIL-9013-2-PC.raw 0072_WUG_FARMM_LIP-9013-2-PC.raw 0072_WU_FARMM_CMH_9013-2-PC.raw 0072_WUG_FARMM_FFA-9013-2-PC.raw SUBJECT_SAMPLE_FACTORS 9013 9013-2-PD Study_Diet:Modulen Type=Plasma; Age=40; Sex=Male; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0071_WUG_FARMM_HIL-9013-2-PD.raw 0071_WUG_FARMM_LIP-9013-2-PD.raw 0071_WU_FARMM_CMH_9013-2-PD.raw 0071_WUG_FARMM_FFA-9013-2-PD.raw SUBJECT_SAMPLE_FACTORS 9013 9013-2-PE Study_Diet:Modulen Type=Plasma; Age=40; Sex=Male; BMI=24.1; Ethnicity=Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0073_WUG_FARMM_HIL-9013-2-PE.raw 0073_WUG_FARMM_LIP-9013-2-PE.raw 0073_WU_FARMM_CMH_9013-2-PE.raw 0073_WUG_FARMM_FFA-9013-2-PE.raw SUBJECT_SAMPLE_FACTORS 9014 9014-3-PA Study_Diet:Vegan Type=Plasma; Age=23; Sex=Female; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0010_WUG_FARMM_HIL-9014-3-PA.raw 0010_WUG_FARMM_LIP-9014-3-PA.raw 0010_WU_FARMM_CMH_9014-3-PA.raw 0010_WUG_FARMM_FFA-9014-3-PA.raw SUBJECT_SAMPLE_FACTORS 9014 9014-3-PB Study_Diet:Vegan Type=Plasma; Age=23; Sex=Female; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0008_WUG_FARMM_HIL-9014-3-PB.raw 0008_WUG_FARMM_LIP-9014-3-PB.raw 0008_WU_FARMM_CMH_9014-3-PB.raw 0008_WUG_FARMM_FFA-9014-3-PB.raw SUBJECT_SAMPLE_FACTORS 9014 9014-3-PC Study_Diet:Vegan Type=Plasma; Age=23; Sex=Female; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0006_WUG_FARMM_HIL-9014-3-PC.raw 0006_WUG_FARMM_LIP-9014-3-PC.raw 0006_WU_FARMM_CMH_9014-3-PC.raw 0006_WUG_FARMM_FFA-9014-3-PC.raw SUBJECT_SAMPLE_FACTORS 9014 9014-3-PD Study_Diet:Vegan Type=Plasma; Age=23; Sex=Female; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0009_WUG_FARMM_HIL-9014-3-PD.raw 0009_WUG_FARMM_LIP-9014-3-PD.raw 0009_WU_FARMM_CMH_9014-3-PD.raw 0009_WUG_FARMM_FFA-9014-3-PD.raw SUBJECT_SAMPLE_FACTORS 9014 9014-3-PE Study_Diet:Vegan Type=Plasma; Age=23; Sex=Female; BMI=20.9; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0007_WUG_FARMM_HIL-9014-3-PE.raw 0007_WUG_FARMM_LIP-9014-3-PE.raw 0007_WU_FARMM_CMH_9014-3-PE.raw 0007_WUG_FARMM_FFA-9014-3-PE.raw SUBJECT_SAMPLE_FACTORS 9015 9015-1-PA Study_Diet:Western Type=Plasma; Age=23; Sex=Female; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0097_WUG_FARMM_HIL-9015-1-PA.raw 0097_WUG_FARMM_LIP-9015-1-PA.raw 0097_WU_FARMM_CMH_9015-1-PA.raw 0097_WUG_FARMM_FFA-9015-1-PA.raw SUBJECT_SAMPLE_FACTORS 9015 9015-1-PB Study_Diet:Western Type=Plasma; Age=23; Sex=Female; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0096_WUG_FARMM_HIL-9015-1-PB.raw 0096_WUG_FARMM_LIP-9015-1-PB.raw 0096_WU_FARMM_CMH_9015-1-PB.raw 0096_WUG_FARMM_FFA-9015-1-PB.raw SUBJECT_SAMPLE_FACTORS 9015 9015-1-PC Study_Diet:Western Type=Plasma; Age=23; Sex=Female; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0100_WUG_FARMM_HIL-9015-1-PC.raw 0100_WUG_FARMM_LIP-9015-1-PC.raw 0100_WU_FARMM_CMH_9015-1-PC.raw 0100_WUG_FARMM_FFA-9015-1-PC.raw SUBJECT_SAMPLE_FACTORS 9015 9015-1-PD Study_Diet:Western Type=Plasma; Age=23; Sex=Female; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0099_WUG_FARMM_HIL-9015-1-PD.raw 0099_WUG_FARMM_LIP-9015-1-PD.raw 0099_WU_FARMM_CMH_9015-1-PD.raw 0099_WUG_FARMM_FFA-9015-1-PD.raw SUBJECT_SAMPLE_FACTORS 9015 9015-1-PE Study_Diet:Western Type=Plasma; Age=23; Sex=Female; BMI=20.5; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0098_WUG_FARMM_HIL-9015-1-PE.raw 0098_WUG_FARMM_LIP-9015-1-PE.raw 0098_WU_FARMM_CMH_9015-1-PE.raw 0098_WUG_FARMM_FFA-9015-1-PE.raw SUBJECT_SAMPLE_FACTORS 9016 9016-2-PA Study_Diet:Modulen Type=Plasma; Age=38; Sex=Female; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0067_WUG_FARMM_HIL-9016-2-PA.raw 0067_WUG_FARMM_LIP-9016-2-PA.raw 0067_WU_FARMM_CMH_9016-2-PA.raw 0067_WUG_FARMM_FFA-9016-2-PA.raw SUBJECT_SAMPLE_FACTORS 9016 9016-2-PB Study_Diet:Modulen Type=Plasma; Age=38; Sex=Female; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0066_WUG_FARMM_HIL-9016-2-PB.raw 0066_WUG_FARMM_LIP-9016-2-PB.raw 0066_WU_FARMM_CMH_9016-2-PB.raw 0066_WUG_FARMM_FFA-9016-2-PB.raw SUBJECT_SAMPLE_FACTORS 9016 9016-2-PC Study_Diet:Modulen Type=Plasma; Age=38; Sex=Female; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0070_WUG_FARMM_HIL-9016-2-PC.raw 0070_WUG_FARMM_LIP-9016-2-PC.raw 0070_WU_FARMM_CMH_9016-2-PC.raw 0070_WUG_FARMM_FFA-9016-2-PC.raw SUBJECT_SAMPLE_FACTORS 9016 9016-2-PD Study_Diet:Modulen Type=Plasma; Age=38; Sex=Female; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0068_WUG_FARMM_HIL-9016-2-PD.raw 0068_WUG_FARMM_LIP-9016-2-PD.raw 0068_WU_FARMM_CMH_9016-2-PD.raw 0068_WUG_FARMM_FFA-9016-2-PD.raw SUBJECT_SAMPLE_FACTORS 9016 9016-2-PE Study_Diet:Modulen Type=Plasma; Age=38; Sex=Female; BMI=25.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0069_WUG_FARMM_HIL-9016-2-PE.raw 0069_WUG_FARMM_LIP-9016-2-PE.raw 0069_WU_FARMM_CMH_9016-2-PE.raw 0069_WUG_FARMM_FFA-9016-2-PE.raw SUBJECT_SAMPLE_FACTORS 9017 9017-3-PA Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0057_WUG_FARMM_HIL-9017-3-PA.raw 0057_WUG_FARMM_LIP-9017-3-PA.raw 0057_WU_FARMM_CMH_9017-3-PA.raw 0057_WUG_FARMM_FFA-9017-3-PA.raw SUBJECT_SAMPLE_FACTORS 9017 9017-3-PB Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0056_WUG_FARMM_HIL-9017-3-PB.raw 0056_WUG_FARMM_LIP-9017-3-PB.raw 0056_WU_FARMM_CMH_9017-3-PB.raw 0056_WUG_FARMM_FFA-9017-3-PB.raw SUBJECT_SAMPLE_FACTORS 9017 9017-3-PC Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0058_WUG_FARMM_HIL-9017-3-PC.raw 0058_WUG_FARMM_LIP-9017-3-PC.raw 0058_WU_FARMM_CMH_9017-3-PC.raw 0058_WUG_FARMM_FFA-9017-3-PC.raw SUBJECT_SAMPLE_FACTORS 9017 9017-3-PD Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0060_WUG_FARMM_HIL-9017-3-PD.raw 0060_WUG_FARMM_LIP-9017-3-PD.raw 0060_WU_FARMM_CMH_9017-3-PD.raw 0060_WUG_FARMM_FFA-9017-3-PD.raw SUBJECT_SAMPLE_FACTORS 9017 9017-3-PE Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=20.6; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0059_WUG_FARMM_HIL-9017-3-PE.raw 0059_WUG_FARMM_LIP-9017-3-PE.raw 0059_WU_FARMM_CMH_9017-3-PE.raw 0059_WUG_FARMM_FFA-9017-3-PE.raw SUBJECT_SAMPLE_FACTORS 9019 9019-1-PA Study_Diet:Western Type=Plasma; Age=36; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 15; RAW_FILE_NAME=0061_WUG_FARMM_HIL-9019-1-PA.raw 0061_WUG_FARMM_LIP-9019-1-PA.raw 0061_WU_FARMM_CMH_9019-1-PA.raw 0061_WUG_FARMM_FFA-9019-1-PA.raw SUBJECT_SAMPLE_FACTORS 9019 9019-1-PB Study_Diet:Western Type=Plasma; Age=36; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 12; RAW_FILE_NAME=0063_WUG_FARMM_HIL-9019-1-PB.raw 0063_WUG_FARMM_LIP-9019-1-PB.raw 0063_WU_FARMM_CMH_9019-1-PB.raw 0063_WUG_FARMM_FFA-9019-1-PB.raw SUBJECT_SAMPLE_FACTORS 9019 9019-1-PC Study_Diet:Western Type=Plasma; Age=36; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 9; RAW_FILE_NAME=0064_WUG_FARMM_HIL-9019-1-PC.raw 0064_WUG_FARMM_LIP-9019-1-PC.raw 0064_WU_FARMM_CMH_9019-1-PC.raw 0064_WUG_FARMM_FFA-9019-1-PC.raw SUBJECT_SAMPLE_FACTORS 9019 9019-1-PD Study_Diet:Western Type=Plasma; Age=36; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 5; RAW_FILE_NAME=0062_WUG_FARMM_HIL-9019-1-PD.raw 0062_WUG_FARMM_LIP-9019-1-PD.raw 0062_WU_FARMM_CMH_9019-1-PD.raw 0062_WUG_FARMM_FFA-9019-1-PD.raw SUBJECT_SAMPLE_FACTORS 9019 9019-1-PE Study_Diet:Western Type=Plasma; Age=36; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Baseline; RAW_FILE_NAME=0065_WUG_FARMM_HIL-9019-1-PE.raw 0065_WUG_FARMM_LIP-9019-1-PE.raw 0065_WU_FARMM_CMH_9019-1-PE.raw 0065_WUG_FARMM_FFA-9019-1-PE.raw SUBJECT_SAMPLE_FACTORS 9022 9022-3-PA Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0005_WUG_FARMM_HIL-9022-3-PA.raw 0005_WUG_FARMM_LIP-9022-3-PA.raw 0005_WU_FARMM_CMH_9022-3-PA.raw 0005_WUG_FARMM_FFA-9022-3-PA.raw SUBJECT_SAMPLE_FACTORS 9022 9022-3-PB Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0003_WUG_FARMM_HIL-9022-3-PB.raw 0003_WUG_FARMM_LIP-9022-3-PB.raw 0003_WU_FARMM_CMH_9022-3-PB.raw 0003_WUG_FARMM_FFA-9022-3-PB.raw SUBJECT_SAMPLE_FACTORS 9022 9022-3-PC Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0004_WUG_FARMM_HIL-9022-3-PC.raw 0004_WUG_FARMM_LIP-9022-3-PC.raw 0004_WU_FARMM_CMH_9022-3-PC.raw 0004_WUG_FARMM_FFA-9022-3-PC.raw SUBJECT_SAMPLE_FACTORS 9022 9022-3-PD Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0002_WUG_FARMM_HIL-9022-3-PD.raw 0002_WUG_FARMM_LIP-9022-3-PD.raw 0002_WU_FARMM_CMH_9022-3-PD.raw 0002_WUG_FARMM_FFA-9022-3-PD.raw SUBJECT_SAMPLE_FACTORS 9022 9022-3-PE Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=21.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0001_WUG_FARMM_HIL-9022-3.raw 0001_WUG_FARMM_LIP-9022-3-PE.raw 0001_WU_FARMM_CMH_9022-3-PE.raw 0001_WUG_FARMM_FFA-9022-3-PE.raw SUBJECT_SAMPLE_FACTORS 9024 9024-2-PA Study_Diet:Modulen Type=Plasma; Age=27; Sex=Female; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 15; RAW_FILE_NAME=0012_WUG_FARMM_HIL-9024-2-PA.raw 0012_WUG_FARMM_LIP-9024-2-PA.raw 0012_WU_FARMM_CMH_9024-2-PA.raw 0012_WUG_FARMM_FFA-9024-2-PA.raw SUBJECT_SAMPLE_FACTORS 9024 9024-2-PB Study_Diet:Modulen Type=Plasma; Age=27; Sex=Female; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 12; RAW_FILE_NAME=0013_WUG_FARMM_HIL-9024-2-PB.raw 0013_WUG_FARMM_LIP-9024-2-PB.raw 0013_WU_FARMM_CMH_9024-2-PB.raw 0013_WUG_FARMM_FFA-9024-2-PB.raw SUBJECT_SAMPLE_FACTORS 9024 9024-2-PC Study_Diet:Modulen Type=Plasma; Age=27; Sex=Female; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 9; RAW_FILE_NAME=0015_WUG_FARMM_HIL-9024-2-PC.raw 0015_WUG_FARMM_LIP-9024-2-PC.raw 0015_WU_FARMM_CMH_9024-2-PC.raw 0015_WUG_FARMM_FFA-9024-2-PC.raw SUBJECT_SAMPLE_FACTORS 9024 9024-2-PD Study_Diet:Modulen Type=Plasma; Age=27; Sex=Female; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 5; RAW_FILE_NAME=0014_WUG_FARMM_HIL-9024-2-PD.raw 0014_WUG_FARMM_LIP-9024-2-PD.raw 0014_WU_FARMM_CMH_9024-2-PD.raw 0014_WUG_FARMM_FFA-9024-2-PD.raw SUBJECT_SAMPLE_FACTORS 9024 9024-2-PE Study_Diet:Modulen Type=Plasma; Age=27; Sex=Female; BMI=34.6; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Baseline; RAW_FILE_NAME=0011_WUG_FARMM_HIL-9024-2-PE.raw 0011_WUG_FARMM_LIP-9024-2-PE.raw 0011_WU_FARMM_CMH_9024-2-PE.raw 0011_WUG_FARMM_FFA-9024-2-PE.raw SUBJECT_SAMPLE_FACTORS 9025 9025-2-PA Study_Diet:Modulen Type=Plasma; Age=28; Sex=Male; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 15; RAW_FILE_NAME=0041_WUG_FARMM_HIL-9025-2-PA.raw 0041_WUG_FARMM_LIP-9025-2-PA.raw 0041_WU_FARMM_CMH_9025-2-PA.raw 0041_WUG_FARMM_FFA-9025-2-PA.raw SUBJECT_SAMPLE_FACTORS 9025 9025-2-PB Study_Diet:Modulen Type=Plasma; Age=28; Sex=Male; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 12; RAW_FILE_NAME=0045_WUG_FARMM_HIL-9025-2-PB.raw 0045_WUG_FARMM_LIP-9025-2-PB.raw 0045_WU_FARMM_CMH_9025-2-PB.raw 0045_WUG_FARMM_FFA-9025-2-PB.raw SUBJECT_SAMPLE_FACTORS 9025 9025-2-PC Study_Diet:Modulen Type=Plasma; Age=28; Sex=Male; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 9; RAW_FILE_NAME=0043_WUG_FARMM_HIL-9025-2-PC.raw 0043_WUG_FARMM_LIP-9025-2-PC.raw 0043_WU_FARMM_CMH_9025-2-PC.raw 0043_WUG_FARMM_FFA-9025-2-PC.raw SUBJECT_SAMPLE_FACTORS 9025 9025-2-PD Study_Diet:Modulen Type=Plasma; Age=28; Sex=Male; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 5; RAW_FILE_NAME=0044_WUG_FARMM_HIL-9025-2-PD.raw 0044_WUG_FARMM_LIP-9025-2-PD.raw 0044_WU_FARMM_CMH_9025-2-PD.raw 0044_WUG_FARMM_FFA-9025-2-PD.raw SUBJECT_SAMPLE_FACTORS 9025 9025-2-PE Study_Diet:Modulen Type=Plasma; Age=28; Sex=Male; BMI=30.8; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Baseline; RAW_FILE_NAME=0042_WUG_FARMM_HIL-9025-2-PE.raw 0042_WUG_FARMM_LIP-9025-2-PE.raw 0042_WU_FARMM_CMH_9025-2-PE.raw 0042_WUG_FARMM_FFA-9025-2-PE.raw SUBJECT_SAMPLE_FACTORS 9026 9026-3-PA Study_Diet:Vegan Type=Plasma; Age=24; Sex=Female; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 15; RAW_FILE_NAME=0039_WUG_FARMM_HIL-9026-3-PA.raw 0039_WUG_FARMM_LIP-9026-3-PA.raw 0039_WU_FARMM_CMH_9026-3-PA.raw 0039_WUG_FARMM_FFA-9026-3-PA.raw SUBJECT_SAMPLE_FACTORS 9026 9026-3-PB Study_Diet:Vegan Type=Plasma; Age=24; Sex=Female; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 12; RAW_FILE_NAME=0040_WUG_FARMM_HIL-9026-3-PB.raw 0040_WUG_FARMM_LIP-9026-3-PB.raw 0040_WU_FARMM_CMH_9026-3-PB.raw 0040_WUG_FARMM_FFA-9026-3-PB.raw SUBJECT_SAMPLE_FACTORS 9026 9026-3-PC Study_Diet:Vegan Type=Plasma; Age=24; Sex=Female; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 9; RAW_FILE_NAME=0038_WUG_FARMM_HIL-9026-3-PC.raw 0038_WUG_FARMM_LIP-9026-3-PC.raw 0038_WU_FARMM_CMH_9026-3-PC.raw 0038_WUG_FARMM_FFA-9026-3-PC.raw SUBJECT_SAMPLE_FACTORS 9026 9026-3-PD Study_Diet:Vegan Type=Plasma; Age=24; Sex=Female; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Day 5; RAW_FILE_NAME=0036_WUG_FARMM_HIL-9026-3-PD.raw 0036_WUG_FARMM_LIP-9026-3-PD.raw 0036_WU_FARMM_CMH_9026-3-PD.raw 0036_WUG_FARMM_FFA-9026-3-PD.raw SUBJECT_SAMPLE_FACTORS 9026 9026-3-PE Study_Diet:Vegan Type=Plasma; Age=24; Sex=Female; BMI=21.3; Ethnicity=Not Hispanic or Latino; Race=Black/African American; Time=Baseline; RAW_FILE_NAME=0037_WUG_FARMM_HIL-9026-3-PE.raw 0037_WUG_FARMM_LIP-9026-3-PE.raw 0037_WU_FARMM_CMH_9026-3-PE.raw 0037_WUG_FARMM_FFA-9026-3-PE.raw SUBJECT_SAMPLE_FACTORS 9027 9027-3-PA Study_Diet:Vegan Type=Plasma; Age=22; Sex=Female; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0030_WUG_FARMM_HIL-9027-3-PA.raw 0030_WUG_FARMM_LIP-9027-3-PA.raw 0030_WU_FARMM_CMH_9027-3-PA.raw 0030_WUG_FARMM_FFA-9027-3-PA.raw SUBJECT_SAMPLE_FACTORS 9027 9027-3-PB Study_Diet:Vegan Type=Plasma; Age=22; Sex=Female; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0028_WUG_FARMM_HIL-9027-3-PB.raw 0028_WUG_FARMM_LIP-9027-3-PB.raw 0028_WU_FARMM_CMH_9027-3-PB.raw 0028_WUG_FARMM_FFA-9027-3-PB.raw SUBJECT_SAMPLE_FACTORS 9027 9027-3-PC Study_Diet:Vegan Type=Plasma; Age=22; Sex=Female; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0029_WUG_FARMM_HIL-9027-3-PC.raw 0029_WUG_FARMM_LIP-9027-3-PC.raw 0029_WU_FARMM_CMH_9027-3-PC.raw 0029_WUG_FARMM_FFA-9027-3-PC.raw SUBJECT_SAMPLE_FACTORS 9027 9027-3-PD Study_Diet:Vegan Type=Plasma; Age=22; Sex=Female; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0026_WUG_FARMM_HIL-9027-3-PD.raw 0026_WUG_FARMM_LIP-9027-3-PD.raw 0026_WU_FARMM_CMH_9027-3-PD.raw 0026_WUG_FARMM_FFA-9027-3-PD.raw SUBJECT_SAMPLE_FACTORS 9027 9027-3-PE Study_Diet:Vegan Type=Plasma; Age=22; Sex=Female; BMI=25.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0027_WUG_FARMM_HIL-9027-3-PE.raw 0027_WUG_FARMM_LIP-9027-3-PE.raw 0027_WU_FARMM_CMH_9027-3-PE.raw 0027_WUG_FARMM_FFA-9027-3-PE.raw SUBJECT_SAMPLE_FACTORS 9029 9029-2-PA Study_Diet:Modulen Type=Plasma; Age=26; Sex=Male; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0128_WUG_FARMM_HIL-9029-2-PA.raw 0128_WUG_FARMM_LIP-9029-2-PA.raw 0128_WU_FARMM_CMH_9029-2-PA.raw 0128_WUG_FARMM_FFA-9029-2-PA.raw SUBJECT_SAMPLE_FACTORS 9029 9029-2-PB Study_Diet:Modulen Type=Plasma; Age=26; Sex=Male; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0126_WUG_FARMM_HIL-9029-2-PB.raw 0126_WUG_FARMM_LIP-9029-2-PB.raw 0126_WU_FARMM_CMH_9029-2-PB.raw 0126_WUG_FARMM_FFA-9029-2-PB.raw SUBJECT_SAMPLE_FACTORS 9029 9029-2-PC Study_Diet:Modulen Type=Plasma; Age=26; Sex=Male; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0130_WUG_FARMM_HIL-9029-2-PC.raw 0130_WUG_FARMM_LIP-9029-2-PC.raw 0130_WU_FARMM_CMH_9029-2-PC.raw 0130_WUG_FARMM_FFA-9029-2-PC.raw SUBJECT_SAMPLE_FACTORS 9029 9029-2-PD Study_Diet:Modulen Type=Plasma; Age=26; Sex=Male; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0127_WUG_FARMM_HIL-9029-2-PD.raw 0127_WUG_FARMM_LIP-9029-2-PD.raw 0127_WU_FARMM_CMH_9029-2-PD.raw 0127_WUG_FARMM_FFA-9029-2-PD.raw SUBJECT_SAMPLE_FACTORS 9029 9029-2-PE Study_Diet:Modulen Type=Plasma; Age=26; Sex=Male; BMI=35.4; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0129_WUG_FARMM_HIL-9029-2-PE.raw 0129_WUG_FARMM_LIP-9029-2-PE.raw 0129_WU_FARMM_CMH_9029-2-PE.raw 0129_WUG_FARMM_FFA-9029-2-PE.raw SUBJECT_SAMPLE_FACTORS 9030 9030-1-PA Study_Diet:Western Type=Plasma; Age=46; Sex=Male; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0053_WUG_FARMM_HIL-9030-1-PA.raw 0053_WUG_FARMM_LIP-9030-1-PA.raw 0053_WU_FARMM_CMH_9030-1-PA.raw 0053_WUG_FARMM_FFA-9030-1-PA.raw SUBJECT_SAMPLE_FACTORS 9030 9030-1-PB Study_Diet:Western Type=Plasma; Age=46; Sex=Male; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0054_WUG_FARMM_HIL-9030-1-PB.raw 0054_WUG_FARMM_LIP-9030-1-PB.raw 0054_WU_FARMM_CMH_9030-1-PB.raw 0054_WUG_FARMM_FFA-9030-1-PB.raw SUBJECT_SAMPLE_FACTORS 9030 9030-1-PC Study_Diet:Western Type=Plasma; Age=46; Sex=Male; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0055_WUG_FARMM_HIL-9030-1-PC.raw 0055_WUG_FARMM_LIP-9030-1-PC.raw 0055_WU_FARMM_CMH_9030-1-PC.raw 0055_WUG_FARMM_FFA-9030-1-PC.raw SUBJECT_SAMPLE_FACTORS 9030 9030-1-PD Study_Diet:Western Type=Plasma; Age=46; Sex=Male; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0051_WUG_FARMM_HIL-9030-1-PD.raw 0051_WUG_FARMM_LIP-9030-1-PD.raw 0051_WU_FARMM_CMH_9030-1-PD.raw 0051_WUG_FARMM_FFA-9030-1-PD.raw SUBJECT_SAMPLE_FACTORS 9030 9030-1-PE Study_Diet:Western Type=Plasma; Age=46; Sex=Male; BMI=27.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0052_WUG_FARMM_HIL-9030-1-PE.raw 0052_WUG_FARMM_LIP-9030-1-PE.raw 0052_WU_FARMM_CMH_9030-1-PE.raw 0052_WUG_FARMM_FFA-9030-1-PE.raw SUBJECT_SAMPLE_FACTORS 9031 9031-3-PA Study_Diet:Vegan Type=Plasma; Age=32; Sex=Female; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0019_WUG_FARMM_HIL-9031-3-PA.raw 0019_WUG_FARMM_LIP-9031-3-PA.raw 0019_WU_FARMM_CMH_9031-3-PA.raw 0019_WUG_FARMM_FFA-9031-3-PA.raw SUBJECT_SAMPLE_FACTORS 9031 9031-3-PB Study_Diet:Vegan Type=Plasma; Age=32; Sex=Female; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0018_WUG_FARMM_HIL-9031-3-PB.raw 0018_WUG_FARMM_LIP-9031-3-PB.raw 0018_WU_FARMM_CMH_9031-3-PB.raw 0018_WUG_FARMM_FFA-9031-3-PB.raw SUBJECT_SAMPLE_FACTORS 9031 9031-3-PC Study_Diet:Vegan Type=Plasma; Age=32; Sex=Female; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0020_WUG_FARMM_HIL-9031-3-PC.raw 0020_WUG_FARMM_LIP-9031-3-PC.raw 0020_WU_FARMM_CMH_9031-3-PC.raw 0020_WUG_FARMM_FFA-9031-3-PC.raw SUBJECT_SAMPLE_FACTORS 9031 9031-3-PD Study_Diet:Vegan Type=Plasma; Age=32; Sex=Female; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0016_WUG_FARMM_HIL-9031-3-PD.raw 0016_WUG_FARMM_LIP-9031-3-PD.raw 0016_WU_FARMM_CMH_9031-3-PD.raw 0016_WUG_FARMM_FFA-9031-3-PD.raw SUBJECT_SAMPLE_FACTORS 9031 9031-3-PE Study_Diet:Vegan Type=Plasma; Age=32; Sex=Female; BMI=22.8; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0017_WUG_FARMM_HIL-9031-3-PE.raw 0017_WUG_FARMM_LIP-9031-3-PE.raw 0017_WU_FARMM_CMH_9031-3-PE.raw 0017_WUG_FARMM_FFA-9031-3-PE.raw SUBJECT_SAMPLE_FACTORS 9032 9032-2-PA Study_Diet:Modulen Type=Plasma; Age=53; Sex=Male; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0111_WUG_FARMM_HIL-9032-2-PA.raw 0111_WUG_FARMM_LIP-9032-2-PA.raw 0111_WU_FARMM_CMH_9032-2-PA.raw 0111_WUG_FARMM_FFA-9032-2-PA.raw SUBJECT_SAMPLE_FACTORS 9032 9032-2-PB Study_Diet:Modulen Type=Plasma; Age=53; Sex=Male; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0114_WUG_FARMM_HIL-9032-2-PB.raw 0114_WUG_FARMM_LIP-9032-2-PB.raw 0114_WU_FARMM_CMH_9032-2-PB.raw 0114_WUG_FARMM_FFA-9032-2-PB.raw SUBJECT_SAMPLE_FACTORS 9032 9032-2-PC Study_Diet:Modulen Type=Plasma; Age=53; Sex=Male; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0112_WUG_FARMM_HIL-9032-2-PC.raw 0112_WUG_FARMM_LIP-9032-2-PC.raw 0112_WU_FARMM_CMH_9032-2-PC.raw 0112_WUG_FARMM_FFA-9032-2-PC.raw SUBJECT_SAMPLE_FACTORS 9032 9032-2-PD Study_Diet:Modulen Type=Plasma; Age=53; Sex=Male; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0113_WUG_FARMM_HIL-9032-2-PD.raw 0113_WUG_FARMM_LIP-9032-2-PD.raw 0113_WU_FARMM_CMH_9032-2-PD.raw 0113_WUG_FARMM_FFA-9032-2-PD.raw SUBJECT_SAMPLE_FACTORS 9032 9032-2-PE Study_Diet:Modulen Type=Plasma; Age=53; Sex=Male; BMI=31; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0115_WUG_FARMM_HIL-9032-2-PE.raw 0115_WUG_FARMM_LIP-9032-2-PE.raw 0115_WU_FARMM_CMH_9032-2-PE.raw 0115_WUG_FARMM_FFA-9032-2-PE.raw SUBJECT_SAMPLE_FACTORS 9033 9033-1-PA Study_Diet:Western Type=Plasma; Age=57; Sex=Male; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0033_WUG_FARMM_HIL-9033-1-PA.raw 0033_WUG_FARMM_LIP-9033-1-PA.raw 0033_WU_FARMM_CMH_9033-1-PA.raw 0033_WUG_FARMM_FFA-9033-1-PA.raw SUBJECT_SAMPLE_FACTORS 9033 9033-1-PB Study_Diet:Western Type=Plasma; Age=57; Sex=Male; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0031_WUG_FARMM_HIL-9033-1-PB.raw 0031_WUG_FARMM_LIP-9033-1-PB.raw 0031_WU_FARMM_CMH_9033-1-PB.raw 0031_WUG_FARMM_FFA-9033-1-PB.raw SUBJECT_SAMPLE_FACTORS 9033 9033-1-PC Study_Diet:Western Type=Plasma; Age=57; Sex=Male; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0034_WUG_FARMM_HIL-9033-1-PC.raw 0034_WUG_FARMM_LIP-9033-1-PC.raw 0034_WU_FARMM_CMH_9033-1-PC.raw 0034_WUG_FARMM_FFA-9033-1-PC.raw SUBJECT_SAMPLE_FACTORS 9033 9033-1-PD Study_Diet:Western Type=Plasma; Age=57; Sex=Male; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0035_WUG_FARMM_HIL-9033-1-PD.raw 0035_WUG_FARMM_LIP-9033-1-PD.raw 0035_WU_FARMM_CMH_9033-1-PD.raw 0035_WUG_FARMM_FFA-9033-1-PD.raw SUBJECT_SAMPLE_FACTORS 9033 9033-1-PE Study_Diet:Western Type=Plasma; Age=57; Sex=Male; BMI=27.1; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0032_WUG_FARMM_HIL-9033-1-PE.raw 0032_WUG_FARMM_LIP-9033-1-PE.raw 0032_WU_FARMM_CMH_9033-1-PE.raw 0032_WUG_FARMM_FFA-9033-1-PE.raw SUBJECT_SAMPLE_FACTORS 9034 9034-2-PA Study_Diet:Modulen Type=Plasma; Age=43; Sex=Female; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0024_WUG_FARMM_HIL-9034-2-PA.raw 0024_WUG_FARMM_LIP-9034-2-PA.raw 0024_WU_FARMM_CMH_9034-2-PA.raw 0024_WUG_FARMM_FFA-9034-2-PA.raw SUBJECT_SAMPLE_FACTORS 9034 9034-2-PB Study_Diet:Modulen Type=Plasma; Age=43; Sex=Female; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0023_WUG_FARMM_HIL-9034-2-PB.raw 0023_WUG_FARMM_LIP-9034-2-PB.raw 0023_WU_FARMM_CMH_9034-2-PB.raw 0023_WUG_FARMM_FFA-9034-2-PB.raw SUBJECT_SAMPLE_FACTORS 9034 9034-2-PC Study_Diet:Modulen Type=Plasma; Age=43; Sex=Female; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0025_WUG_FARMM_HIL-9034-2-PC.raw 0025_WUG_FARMM_LIP-9034-2-PC.raw 0025_WU_FARMM_CMH_9034-2-PC.raw 0025_WUG_FARMM_FFA-9034-2-PC.raw SUBJECT_SAMPLE_FACTORS 9034 9034-2-PD Study_Diet:Modulen Type=Plasma; Age=43; Sex=Female; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0021_WUG_FARMM_HIL-9034-2-PD.raw 0021_WUG_FARMM_LIP-9034-2-PD.raw 0021_WU_FARMM_CMH_9034-2-PD.raw 0021_WUG_FARMM_FFA-9034-2-PD.raw SUBJECT_SAMPLE_FACTORS 9034 9034-2-PE Study_Diet:Modulen Type=Plasma; Age=43; Sex=Female; BMI=25.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0022_WUG_FARMM_HIL-9034-2-PE.raw 0022_WUG_FARMM_LIP-9034-2-PE.raw 0022_WU_FARMM_CMH_9034-2-PE.raw 0022_WUG_FARMM_FFA-9034-2-PE.raw SUBJECT_SAMPLE_FACTORS 9035 9035-3-PA Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0140_WUG_FARMM_HIL-9035-3-PA.raw 0140_WUG_FARMM_LIP-9035-3-PA.raw 0140_WU_FARMM_CMH_9035-3-PA.raw 0140_WUG_FARMM_FFA-9035-3-PA.raw SUBJECT_SAMPLE_FACTORS 9035 9035-3-PB Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0138_WUG_FARMM_HIL-9035-3-PB.raw 0138_WUG_FARMM_LIP-9035-3-PB.raw 0138_WU_FARMM_CMH_9035-3-PB.raw 0138_WUG_FARMM_FFA-9035-3-PB.raw SUBJECT_SAMPLE_FACTORS 9035 9035-3-PC Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0137_WUG_FARMM_HIL-9035-3-PC.raw 0137_WUG_FARMM_LIP-9035-3-PC.raw 0137_WU_FARMM_CMH_9035-3-PC.raw 0137_WUG_FARMM_FFA-9035-3-PC.raw SUBJECT_SAMPLE_FACTORS 9035 9035-3-PD Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0136_WUG_FARMM_HIL-9035-3-PD.raw 0136_WUG_FARMM_LIP-9035-3-PD.raw 0136_WU_FARMM_CMH_9035-3-PD.raw 0136_WUG_FARMM_FFA-9035-3-PD.raw SUBJECT_SAMPLE_FACTORS 9035 9035-3-PE Study_Diet:Vegan Type=Plasma; Age=25; Sex=Male; BMI=19.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0139_WUG_FARMM_HIL-9035-3-PE.raw 0139_WUG_FARMM_LIP-9035-3-PE.raw 0139_WU_FARMM_CMH_9035-3-PE.raw 0139_WUG_FARMM_FFA-9035-3-PE.raw SUBJECT_SAMPLE_FACTORS 9036 9036-1-PA Study_Diet:Western Type=Plasma; Age=60; Sex=Male; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native; Time=White; RAW_FILE_NAME=Day 15;0081_WUG_FARMM_HIL-9036-1-PA.raw 0081_WUG_FARMM_LIP-9036-1-PA.raw 0081_WU_FARMM_CMH_9036-1-PA.raw SUBJECT_SAMPLE_FACTORS 9036 9036-1-PB Study_Diet:Western Type=Plasma; Age=60; Sex=Male; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native; Time=White; RAW_FILE_NAME=Day 12;0083_WUG_FARMM_HIL-9036-1-PB.raw 0083_WUG_FARMM_LIP-9036-1-PB.raw 0083_WU_FARMM_CMH_9036-1-PB.raw SUBJECT_SAMPLE_FACTORS 9036 9036-1-PC Study_Diet:Western Type=Plasma; Age=60; Sex=Male; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native; Time=White; RAW_FILE_NAME=Day 9;0084_WUG_FARMM_HIL-9036-1-PC.raw 0084_WUG_FARMM_LIP-9036-1-PC.raw 0084_WU_FARMM_CMH_9036-1-PC.raw SUBJECT_SAMPLE_FACTORS 9036 9036-1-PD Study_Diet:Western Type=Plasma; Age=60; Sex=Male; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native; Time=White; RAW_FILE_NAME=Day 5;0085_WUG_FARMM_HIL-9036-1-PD.raw 0085_WUG_FARMM_LIP-9036-1-PD.raw 0085_WU_FARMM_CMH_9036-1-PD.raw SUBJECT_SAMPLE_FACTORS 9036 9036-1-PE Study_Diet:Western Type=Plasma; Age=60; Sex=Male; BMI=23.9; Ethnicity=Not Hispanic or Latino; Race=American Indian/Alaskan Native; Time=White; RAW_FILE_NAME=Baseline;0082_WUG_FARMM_HIL-9036-1-PE.raw 0082_WUG_FARMM_LIP-9036-1-PE.raw 0082_WU_FARMM_CMH_9036-1-PE.raw SUBJECT_SAMPLE_FACTORS 9037 9037-1-PA Study_Diet:Western Type=Plasma; Age=29; Sex=Male; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American; Time=White; RAW_FILE_NAME=Day 15;0123_WUG_FARMM_HIL-9037-1-PA.raw 0123_WUG_FARMM_LIP-9037-1-PA.raw 0123_WU_FARMM_CMH_9037-1-PA.raw SUBJECT_SAMPLE_FACTORS 9037 9037-1-PB Study_Diet:Western Type=Plasma; Age=29; Sex=Male; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American; Time=White; RAW_FILE_NAME=Day 12;0125_WUG_FARMM_HIL-9037-1-PB.raw 0125_WUG_FARMM_LIP-9037-1-PB.raw 0125_WU_FARMM_CMH_9037-1-PB.raw SUBJECT_SAMPLE_FACTORS 9037 9037-1-PC Study_Diet:Western Type=Plasma; Age=29; Sex=Male; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American; Time=White; RAW_FILE_NAME=Day 9;0121_WUG_FARMM_HIL-9037-1-PC.raw 0121_WUG_FARMM_LIP-9037-1-PC.raw 0121_WU_FARMM_CMH_9037-1-PC.raw SUBJECT_SAMPLE_FACTORS 9037 9037-1-PD Study_Diet:Western Type=Plasma; Age=29; Sex=Male; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American; Time=White; RAW_FILE_NAME=Day 5;0122_WUG_FARMM_HIL-9037-1-PD.raw 0122_WUG_FARMM_LIP-9037-1-PD.raw 0122_WU_FARMM_CMH_9037-1-PD.raw SUBJECT_SAMPLE_FACTORS 9037 9037-1-PE Study_Diet:Western Type=Plasma; Age=29; Sex=Male; BMI=23; Ethnicity=Hispanic or Latino; Race=Black/African American; Time=White; RAW_FILE_NAME=Baseline;0124_WUG_FARMM_HIL-9037-1-PE.raw 0124_WUG_FARMM_LIP-9037-1-PE.raw 0124_WU_FARMM_CMH_9037-1-PE.raw SUBJECT_SAMPLE_FACTORS 9038 9038-2-PA Study_Diet:Modulen Type=Plasma; Age=59; Sex=Female; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0086_WUG_FARMM_HIL-9038-2-PA.raw 0086_WUG_FARMM_LIP-9038-2-PA.raw 0086_WU_FARMM_CMH_9038-2-PA.raw 0086_WUG_FARMM_FFA-9038-2-PA.raw SUBJECT_SAMPLE_FACTORS 9038 9038-2-PB Study_Diet:Modulen Type=Plasma; Age=59; Sex=Female; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0090_WUG_FARMM_HIL-9038-2-PB.raw 0090_WUG_FARMM_LIP-9038-2-PB.raw 0090_WU_FARMM_CMH_9038-2-PB.raw 0090_WUG_FARMM_FFA-9038-2-PB.raw SUBJECT_SAMPLE_FACTORS 9038 9038-2-PC Study_Diet:Modulen Type=Plasma; Age=59; Sex=Female; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0088_WUG_FARMM_HIL-9038-2-PC.raw 0088_WUG_FARMM_LIP-9038-2-PC.raw 0088_WU_FARMM_CMH_9038-2-PC.raw 0088_WUG_FARMM_FFA-9038-2-PC.raw SUBJECT_SAMPLE_FACTORS 9038 9038-2-PD Study_Diet:Modulen Type=Plasma; Age=59; Sex=Female; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0089_WUG_FARMM_HIL-9038-2-PD.raw 0089_WUG_FARMM_LIP-9038-2-PD.raw 0089_WU_FARMM_CMH_9038-2-PD.raw 0089_WUG_FARMM_FFA-9038-2-PD.raw SUBJECT_SAMPLE_FACTORS 9038 9038-2-PE Study_Diet:Modulen Type=Plasma; Age=59; Sex=Female; BMI=34.3; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0087_WUG_FARMM_HIL-9038-2-PE.raw 0087_WUG_FARMM_LIP-9038-2-PE.raw 0087_WU_FARMM_CMH_9038-2-PE.raw 0087_WUG_FARMM_FFA-9038-2-PE.raw SUBJECT_SAMPLE_FACTORS 9040 9040-1-PA Study_Diet:Western Type=Plasma; Age=22; Sex=Male; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 15; RAW_FILE_NAME=0120_WUG_FARMM_HIL-9040-1-PA.raw 0120_WUG_FARMM_LIP-9040-1-PA.raw 0120_WU_FARMM_CMH_9040-1-PA.raw 0120_WUG_FARMM_FFA-9040-1-PA.raw SUBJECT_SAMPLE_FACTORS 9040 9040-1-PB Study_Diet:Western Type=Plasma; Age=22; Sex=Male; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 12; RAW_FILE_NAME=0116_WUG_FARMM_HIL-9040-1-PB.raw 0116_WUG_FARMM_LIP-9040-1-PB.raw 0116_WU_FARMM_CMH_9040-1-PB.raw 0116_WUG_FARMM_FFA-9040-1-PB.raw SUBJECT_SAMPLE_FACTORS 9040 9040-1-PC Study_Diet:Western Type=Plasma; Age=22; Sex=Male; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 9; RAW_FILE_NAME=0119_WUG_FARMM_HIL-9040-1-PC.raw 0119_WUG_FARMM_LIP-9040-1-PC.raw 0119_WU_FARMM_CMH_9040-1-PC.raw 0119_WUG_FARMM_FFA-9040-1-PC.raw SUBJECT_SAMPLE_FACTORS 9040 9040-1-PD Study_Diet:Western Type=Plasma; Age=22; Sex=Male; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Day 5; RAW_FILE_NAME=0117_WUG_FARMM_HIL-9040-1-PD.raw 0117_WUG_FARMM_LIP-9040-1-PD.raw 0117_WU_FARMM_CMH_9040-1-PD.raw 0117_WUG_FARMM_FFA-9040-1-PD.raw SUBJECT_SAMPLE_FACTORS 9040 9040-1-PE Study_Diet:Western Type=Plasma; Age=22; Sex=Male; BMI=23.7; Ethnicity=Not Hispanic or Latino; Race=White; Time=Baseline; RAW_FILE_NAME=0118_WUG_FARMM_HIL-9040-1-PE.raw 0150f_0118ri_WUG_FARMM_LIP-9040-1-PE.raw 0118_WU_FARMM_CMH_9040-1-PE.raw 0118_WUG_FARMM_FFA-9040-1-PE.raw SUBJECT_SAMPLE_FACTORS QC-pooled_plasma QPP01 Study_Diet:NA Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=NA;NA;0000d_WU_FARMM_CMH_QPP-01.raw NA SUBJECT_SAMPLE_FACTORS QC-pooled_plasma QPP02 Study_Diet:NA Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0020a_WUG_FARMM_HIL-QPP02.raw 0020a_WUG_FARMM_LIP-QCPP02.raw 0020a_WU_FARMM_CMH_QPP-02.raw 0020a_WUG_FARMM_FFA-QCPP02.raw SUBJECT_SAMPLE_FACTORS QC-pooled_plasma QPP03 Study_Diet:NA Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0040a_WUG_FARMM_HIL-QPP03.raw NA;0040a_WU_FARMM_CMH_QPP-03.raw 0040a_WUG_FARMM_FFA-QCPP03.raw SUBJECT_SAMPLE_FACTORS QC-pooled_plasma QPP04 Study_Diet:NA Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0060a_WUG_FARMM_HIL-QPP04.raw 0060a_WUG_FARMM_LIP-QCPP04.raw 0060a_WU_FARMM_CMH_QPP-04.raw 0060a_WUG_FARMM_FFA-QCPP04.raw SUBJECT_SAMPLE_FACTORS QC-pooled_plasma QPP05 Study_Diet:NA Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0080a_WUG_FARMM_HIL-QPP05.raw 0080a_WUG_FARMM_LIP-QCPP05.raw 0080a_WU_FARMM_CMH_QPP-05.raw 0080a_WUG_FARMM_FFA-QCPP05.raw SUBJECT_SAMPLE_FACTORS QC-pooled_plasma QPP06 Study_Diet:NA Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0100a_WUG_FARMM_HIL-QPP06.raw 0100a_WUG_FARMM_LIP-QCPP06.raw 0080d_WU_FARMM_CMH_QPP-06.raw 0100a_WUG_FARMM_FFA-QCPP06.raw SUBJECT_SAMPLE_FACTORS QC-pooled_plasma QPP07 Study_Diet:NA Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0120a_WUG_FARMM_HIL-QPP07.raw NA;0100a_WU_FARMM_CMH_QPP-07.raw 0120a_WUG_FARMM_FFA-QCPP07.raw SUBJECT_SAMPLE_FACTORS QC-pooled_plasma QPP08 Study_Diet:NA Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0140a_WUG_FARMM_HIL-QPP08.raw 0150a_WUG_FARMM_LIP-QCPP08.raw 0120a_WU_FARMM_CMH_QPP-08.raw 0150a_WUG_FARMM_FFA-QCPP08.raw SUBJECT_SAMPLE_FACTORS QC-pooled_plasma QPP09 Study_Diet:NA Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=0150a_WUG_FARMM_HIL-QPP09.raw NA;0140a_WU_FARMM_CMH_QPP-09.raw NA SUBJECT_SAMPLE_FACTORS QC-pooled_plasma QPP10 Study_Diet:NA Type=Plasma; Age=NA; Sex=NA; BMI=NA; Ethnicity=NA; Race=NA; Time=NA; RAW_FILE_NAME=NA;NA;0150a_WU_FARMM_CMH_QPP-10.raw NA #COLLECTION CO:COLLECTION_SUMMARY The first stool sample of each day of the inpatient groups was collected, CO:COLLECTION_SUMMARY aliquoted and frozen immediately at -80oC. Blood was collected on days 1, 5, 9, CO:COLLECTION_SUMMARY 12 and 15 from which plasma aliquots were immediately isolated and frozen at CO:COLLECTION_SUMMARY -80oC. The outpatient participants following the vegan diet also had blood CO:COLLECTION_SUMMARY collected on days 1, 5, 9, 12, and 15 from which plasma aliquots were CO:COLLECTION_SUMMARY immediately isolated and frozen at -80oC. Among these participants, the first CO:COLLECTION_SUMMARY stool of the day was collected at home daily and kept on ice packs until it was CO:COLLECTION_SUMMARY brought to the research unit where it could be aliquoted and frozen. Samples CO:COLLECTION_SUMMARY from vegans were received for aliquoting within 24 hours and on average within 4 CO:COLLECTION_SUMMARY hours (Wu et al., 2010). Day 0 stool was not collected from vegans since their CO:COLLECTION_SUMMARY diet did not change. The aliquoted amounts for all samples ranged from 500 mg to CO:COLLECTION_SUMMARY 1 g. The aliquots were taken from different areas of the sample. Remaining CO:COLLECTION_SUMMARY sample was then collected into one residual 50 ml conical tube with a tongue CO:COLLECTION_SUMMARY depressor. Any remaining stool was discarded. The University of Pennsylvania CO:COLLECTION_SUMMARY Institutional Review Board (IRB) approved the research protocol. CO:SAMPLE_TYPE Blood (plasma) CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY The 10 vegan participants continued to follow their usual diet as outpatients. TR:TREATMENT_SUMMARY All participants completed the Diet History Questionnaire II (DHQ II), a food TR:TREATMENT_SUMMARY frequency questionnaire developed by the Risk Factor Monitoring and Methods TR:TREATMENT_SUMMARY Branch of the National Cancer Institute. The vegan participants also completed TR:TREATMENT_SUMMARY three 24 hour diet recalls with a dietitian in the week prior to starting TR:TREATMENT_SUMMARY antibiotics. 20 omnivores were randomly assigned to receive an omnivore diet or TR:TREATMENT_SUMMARY EEN (Modulen® IBD) while residing in an inpatient research unit. The TR:TREATMENT_SUMMARY macronutrient composition of Modulen® is protein 36g, fat 47g, and carbohydrate TR:TREATMENT_SUMMARY 110g per 1000 Kcal. The two omnivore diets were engineered to have a similar TR:TREATMENT_SUMMARY composition to EEN. All the subjects consumed the menu A on day 11 and menu B on TR:TREATMENT_SUMMARY days 4 and 14. Diets for omnivores were constructed to provide the expected TR:TREATMENT_SUMMARY total calories required per day for the participant to maintain their current TR:TREATMENT_SUMMARY weight and were adjusted if there was weight gain or loss of more than 2.5 TR:TREATMENT_SUMMARY pounds. On days 6, 7, and 8, inpatients participants received vancomycin 500mg TR:TREATMENT_SUMMARY orally every 6 hours and neomycin 1000mg orally every 6 hours. On day 7, TR:TREATMENT_SUMMARY participants consumed 4L of polyethylene glycol (PEG) based bowel purgative TR:TREATMENT_SUMMARY (GoLytely®). Participants in the omnivore and EEN arms left the inpatient TR:TREATMENT_SUMMARY research unit only under the direct supervision of a research staff member. The TR:TREATMENT_SUMMARY vegan outpatients reported to the hospital twice on days 6,7 and 8 to receive TR:TREATMENT_SUMMARY antibiotics and to consume PEG on day 7. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Plasma samples were thawed on ice and aliquoted for metabolite profiling SP:SAMPLEPREP_SUMMARY analyses. Plasma samples were thawed and aliquoted for each LC-MS method. LC-MS SP:SAMPLEPREP_SUMMARY samples were prepared for four profiling methods as follows: HILIC-pos: SP:SAMPLEPREP_SUMMARY Metabolites were extracted by adding 90 μL of 74.9:24.9:0.2 v/v/v SP:SAMPLEPREP_SUMMARY acetonitrile/methanol/formic acid containing stable isotope-labeled internal SP:SAMPLEPREP_SUMMARY standards (valine-d8, Isotec; and phenylalanine-d8, Cambridge Isotope SP:SAMPLEPREP_SUMMARY Laboratories; Andover, MA) to a 10 μL aliquot of stool homogenate or plasma. SP:SAMPLEPREP_SUMMARY Samples were vortexed and then centrifuged (10 min, 9,000 x g, 4°C) to pellet SP:SAMPLEPREP_SUMMARY protein precipitates. Supernatants were transferred to glass autosampler vials SP:SAMPLEPREP_SUMMARY containing inserts for LC-MS analysis. C8-pos: Lipids were extracted by adding SP:SAMPLEPREP_SUMMARY 190 μL of isopropanol containing SP:SAMPLEPREP_SUMMARY 1-dodecanoyl-2-tridecanoyl-sn-glycero-3-phosphocholine as an internal standard SP:SAMPLEPREP_SUMMARY (Avanti Polar Lipids; Alabaster, AL) to a 10 μL aliquot of stool homogenate or SP:SAMPLEPREP_SUMMARY plasma. Samples were vortexed and then centrifuged (10 min, 9,000 x g, ambient SP:SAMPLEPREP_SUMMARY temperature) to pellet protein precipitates. Supernatants were transferred to SP:SAMPLEPREP_SUMMARY glass autosampler vials containing inserts for LC-MS analysis. HILIC-neg: SP:SAMPLEPREP_SUMMARY Metabolites were extracted by adding 120 μL of 80% methanol containing SP:SAMPLEPREP_SUMMARY inosine-15N4, thymine-d4 and glycocholate-d4 internal standards (Cambridge SP:SAMPLEPREP_SUMMARY Isotope Laboratories; Andover, MA) to a 30 μL aliquot of stool homogenate or SP:SAMPLEPREP_SUMMARY plasma. Samples were vortexed and then centrifuged (10 min, 9,000 x g, 4°C) to SP:SAMPLEPREP_SUMMARY pellet protein precipitates. Supernatants were transferred to glass autosampler SP:SAMPLEPREP_SUMMARY vials containing inserts for LC-MS analysis. C18-neg: Metabolites were extracted SP:SAMPLEPREP_SUMMARY by adding 90 μL of methanol containing PGE2-d4 as an internal standard (Cayman SP:SAMPLEPREP_SUMMARY Chemical Co.; Ann Arbor, MI) to a 30 μL aliquot of stool homogenate or plasma. SP:SAMPLEPREP_SUMMARY Samples were vortexed and then centrifuged (10 min, 9,000 x g, 4°C) to pellet SP:SAMPLEPREP_SUMMARY protein precipitates. Supernatants were transferred to glass autosampler vials SP:SAMPLEPREP_SUMMARY containing inserts for LC-MS analysis. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY HILIC-pos: high resolution and accurate mass profiling of polar metabolites CH:CHROMATOGRAPHY_SUMMARY using HILIC and positive ion mode full scan MS CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Shimadzu Nexera X2 CH:COLUMN_NAME Waters Atlantis HILIC (150 x 2 mm, 3 µm) CH:FLOW_GRADIENT linear CH:FLOW_RATE 250 uL/min CH:COLUMN_TEMPERATURE 30 ℃ CH:SOLVENT_A 10 mM Ammonium formate/0.1% Formic acid in water CH:SOLVENT_B 0.1% Formic acid in Acetonitrile CH:INJECTION_TEMPERATURE 4 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Exactive Plus Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS MS analyses were carried out using electrospray ionization in the positive ion MS:MS_COMMENTS mode using full scan analysis over m/z 70-800 at 70,000 resolution and 3 Hz data MS:MS_COMMENTS acquisition rate. Additional MS settings were: ion spray voltage, 3.5 kV; MS:MS_COMMENTS capillary temperature, 350°C; probe heater temperature, 300°C; sheath gas, 40; MS:MS_COMMENTS auxiliary gas, 15; and S-lens RF level 40. All raw data were processed using MS:MS_COMMENTS Progenesis QI software (NonLinear Dynamics) for feature alignment, nontargeted MS:MS_COMMENTS signal detection, and signal integration. Targeted processing of a subset of MS:MS_COMMENTS known metabolites was conducted using TraceFinder software (Thermo Fisher MS:MS_COMMENTS Scientific; Waltham, MA). Compound identities were confirmed using reference MS:MS_COMMENTS standards MS:MS_RESULTS_FILE ST001522_AN002537_Results.txt UNITS:peak area Has m/z:Yes Has RT:Yes RT units:Minutes #END