#METABOLOMICS WORKBENCH wpathmasiri_20230413_064055 DATATRACK_ID:3859 STUDY_ID:ST002559 ANALYSIS_ID:AN004218 VERSION 1 CREATED_ON 04-14-2023 #PROJECT PR:PROJECT_TITLE The ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity PR:PROJECT_TITLE for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (VCSIP PR:PROJECT_TITLE Cohort) PR:PROJECT_TYPE C18 Reversed-Phase Broad Spectrum Metabolomics PR:PROJECT_SUMMARY This project was funded by the NIH Environmental influences on Child Health PR:PROJECT_SUMMARY Outcomes (ECHO) Program. Untargeted metabolomics profiling data was acquired PR:PROJECT_SUMMARY from urine samples provided by 14 ECHO cohorts. PR:INSTITUTE NC HHEAR Hub PR:DEPARTMENT Untargeted Analysis PR:LABORATORY Sumner Lab PR:LAST_NAME Rushing PR:FIRST_NAME Blake PR:ADDRESS Nutrition Research Institute, UNC-CH, 500 Laureate Way, Kannapolis, NC 28081 PR:EMAIL blake_rushing@unc.edu PR:PHONE (704) 282-9838 PR:FUNDING_SOURCE This research was supported by the Environmental influences on Child Health PR:FUNDING_SOURCE Outcomes (ECHO) program, Office of The Director, National Institutes of Health. PR:FUNDING_SOURCE The content is solely the responsibility of the authors and does not necessarily PR:FUNDING_SOURCE represent the official views of the National Institutes of Health. The VCSIP PR:FUNDING_SOURCE ECHO Cohort is supported by the following ECHO Program Collaborators: ECHO PR:FUNDING_SOURCE Coordinating Center: Duke Clinical Research Institute, Durham, North Carolina: PR:FUNDING_SOURCE Smith PB, Newby KL, Benjamin DK; U2C OD023375; ECHO Data Analysis Center: Johns PR:FUNDING_SOURCE Hopkins University Bloomberg School of Public Health, Baltimore, Maryland: PR:FUNDING_SOURCE Jacobson LP; Research Triangle Institute, Durham, North Carolina: Catellier.D PR:FUNDING_SOURCE U24 OD023382; North Carolina Human Health Exposure Analysis Resource Hub: PR:FUNDING_SOURCE Research Triangle Institute: Fennell T, University of North Carolina at Chapel PR:FUNDING_SOURCE Hill: Sumner S, University of North Carolina at Charlotte: Du X; U2C ES030857; PR:FUNDING_SOURCE Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc., PR:FUNDING_SOURCE Rockville, Maryland: O’Brien B; U24 ES026539 PR:DOI http://dx.doi.org/10.21228/M8971G #STUDY ST:STUDY_TITLE The ECHO Cohort Exposome: First Steps using HHEAR Analysis – An Opportunity ST:STUDY_TITLE for ALL ECHO Cohorts to Contribute Type A Samples – Untargeted Analysis (VCSIP ST:STUDY_TITLE Cohort) ST:STUDY_TYPE Prospective Cohort Study ST:STUDY_SUMMARY Maternal smoking during pregnancy negatively effects fetal lung development and ST:STUDY_SUMMARY causes lifelong decreases in offspring pulmonary function and offspring ST:STUDY_SUMMARY respiratory health including increased risk of childhood asthma development. ST:STUDY_SUMMARY The VCSIP cohort was created from 2 RCTs to determine if vitamin C ST:STUDY_SUMMARY supplementation to pregnant smokers could ameliorate some of the negative ST:STUDY_SUMMARY effects of maternal smoking during pregnancy on offspring lung health (JAMA ST:STUDY_SUMMARY 311:2074-2082, 2014; JAMA Pediatr 177:16-24, 2023). Both pre- and postnatal ST:STUDY_SUMMARY tobacco exposures have been carefully monitored and extensive respiratory ST:STUDY_SUMMARY phenotyping on the offspring have been performed. Please contact Cindy McEvoy ST:STUDY_SUMMARY at Mcevoyc@ohsu.edu for questions related to subject characteristics and ST:STUDY_SUMMARY outcomes. This research was supported by the Environmental influences on Child ST:STUDY_SUMMARY Health Outcomes (ECHO) Program, Office of The Director, National Institutes of ST:STUDY_SUMMARY Health. The content is solely the responsibility of the authors and does not ST:STUDY_SUMMARY necessarily represent the official views of the National Institutes of Health. ST:STUDY_SUMMARY VCSIP is an ECHO cohort which is supported by the following ECHO Program ST:STUDY_SUMMARY Collaborators: ECHO Coordinating Center: Duke Clinical Research Institute, ST:STUDY_SUMMARY Durham, North Carolina: Smith PB, Newby KL, Benjamin DK; U2C OD023375 ECHO Data ST:STUDY_SUMMARY Analysis Center: Johns Hopkins University Bloomberg School of Public Health, ST:STUDY_SUMMARY Baltimore, Maryland: Jacobson LP; Research Triangle Institute, Durham, North ST:STUDY_SUMMARY Carolina: Catellier.D U24 OD023382 North Carolina Human Health Exposure ST:STUDY_SUMMARY Analysis Resource Hub: Research Triangle Institute: Fennell T, University of ST:STUDY_SUMMARY North Carolina at Chapel Hill: Sumner S, University of North Carolina at ST:STUDY_SUMMARY Charlotte: Du X; U2C ES030857 Human Health Exposure Analysis Resource ST:STUDY_SUMMARY Coordinating Center: Westat, Inc., Rockville, Maryland: O’Brien B; U24 ST:STUDY_SUMMARY ES026539 ST:INSTITUTE Oregon Health & Science University ST:DEPARTMENT Division of Neuroscience ST:LAST_NAME Spindel ST:FIRST_NAME Eliot ST:ADDRESS 3181 SW Sam Jackson Park Road Portland, OR 97239-3098 ST:EMAIL spindele@ohsu.edu ST:PHONE 503-346-5524 ST:SUBMIT_DATE 2023-04-13 #SUBJECT SU:SUBJECT_TYPE Human SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - VCSIP_B_8 Sample Type:Blank | Batch:1 | Analysis Order:108 RAW_FILE_NAME=VCSIP_B_8 SUBJECT_SAMPLE_FACTORS - VCSIP_B_1 Sample Type:Blank | Batch:1 | Analysis Order:12 RAW_FILE_NAME=VCSIP_B_1 SUBJECT_SAMPLE_FACTORS - VCSIP_B_2 Sample Type:Blank | Batch:1 | Analysis Order:26 RAW_FILE_NAME=VCSIP_B_2 SUBJECT_SAMPLE_FACTORS - VCSIP_B_3 Sample Type:Blank | Batch:1 | Analysis Order:39 RAW_FILE_NAME=VCSIP_B_3 SUBJECT_SAMPLE_FACTORS - VCSIP_B_4 Sample Type:Blank | Batch:1 | Analysis Order:53 RAW_FILE_NAME=VCSIP_B_4 SUBJECT_SAMPLE_FACTORS - VCSIP_B_5 Sample Type:Blank | Batch:1 | Analysis Order:67 RAW_FILE_NAME=VCSIP_B_5 SUBJECT_SAMPLE_FACTORS - VCSIP_B_6 Sample Type:Blank | Batch:1 | Analysis Order:81 RAW_FILE_NAME=VCSIP_B_6 SUBJECT_SAMPLE_FACTORS - VCSIP_B_7 Sample Type:Blank | Batch:1 | Analysis Order:94 RAW_FILE_NAME=VCSIP_B_7 SUBJECT_SAMPLE_FACTORS - VCSIP_B_9 Sample Type:Blank | Batch:2 | Analysis Order:123 RAW_FILE_NAME=VCSIP_B_9 SUBJECT_SAMPLE_FACTORS - VCSIP_B_10 Sample Type:Blank | Batch:2 | Analysis Order:137 RAW_FILE_NAME=VCSIP_B_10 SUBJECT_SAMPLE_FACTORS - VCSIP_B_11 Sample Type:Blank | Batch:2 | Analysis Order:150 RAW_FILE_NAME=VCSIP_B_11 SUBJECT_SAMPLE_FACTORS - VCSIP_B_12 Sample Type:Blank | Batch:2 | Analysis Order:164 RAW_FILE_NAME=VCSIP_B_12 SUBJECT_SAMPLE_FACTORS - VCSIP_B_13 Sample Type:Blank | Batch:2 | Analysis Order:178 RAW_FILE_NAME=VCSIP_B_13 SUBJECT_SAMPLE_FACTORS - VCSIP_B_14 Sample Type:Blank | Batch:2 | Analysis Order:192 RAW_FILE_NAME=VCSIP_B_14 SUBJECT_SAMPLE_FACTORS - VCSIP_B_15 Sample Type:Blank | Batch:2 | Analysis Order:205 RAW_FILE_NAME=VCSIP_B_15 SUBJECT_SAMPLE_FACTORS - VCSIP_B_16 Sample Type:Blank | Batch:2 | Analysis Order:219 RAW_FILE_NAME=VCSIP_B_16 SUBJECT_SAMPLE_FACTORS - VCSIP_B_17 Sample Type:Blank | Batch:3 | Analysis Order:234 RAW_FILE_NAME=VCSIP_B_17 SUBJECT_SAMPLE_FACTORS - VCSIP_B_18 Sample Type:Blank | Batch:3 | Analysis Order:248 RAW_FILE_NAME=VCSIP_B_18 SUBJECT_SAMPLE_FACTORS - VCSIP_B_19 Sample Type:Blank | Batch:3 | Analysis Order:262 RAW_FILE_NAME=VCSIP_B_19 SUBJECT_SAMPLE_FACTORS - VCSIP_B_20 Sample Type:Blank | Batch:3 | Analysis Order:276 RAW_FILE_NAME=VCSIP_B_20 SUBJECT_SAMPLE_FACTORS - VCSIP_CHEAR_U_1 Sample Type:CHEAR Urine Pool | Batch:1 | Analysis Order:14 RAW_FILE_NAME=VCSIP_CHEAR_U_1 SUBJECT_SAMPLE_FACTORS - VCSIP_CHEAR_U_2 Sample Type:CHEAR Urine Pool | Batch:1 | Analysis Order:41 RAW_FILE_NAME=VCSIP_CHEAR_U_2 SUBJECT_SAMPLE_FACTORS - VCSIP_CHEAR_U_3 Sample Type:CHEAR Urine Pool | Batch:1 | Analysis Order:69 RAW_FILE_NAME=VCSIP_CHEAR_U_3 SUBJECT_SAMPLE_FACTORS - VCSIP_CHEAR_U_4 Sample Type:CHEAR Urine Pool | Batch:1 | Analysis Order:96 RAW_FILE_NAME=VCSIP_CHEAR_U_4 SUBJECT_SAMPLE_FACTORS - VCSIP_CHEAR_U_5 Sample Type:CHEAR Urine Pool | Batch:2 | Analysis Order:125 RAW_FILE_NAME=VCSIP_CHEAR_U_5 SUBJECT_SAMPLE_FACTORS - VCSIP_CHEAR_U_6 Sample Type:CHEAR Urine Pool | Batch:2 | Analysis Order:152 RAW_FILE_NAME=VCSIP_CHEAR_U_6 SUBJECT_SAMPLE_FACTORS - VCSIP_CHEAR_U_7 Sample Type:CHEAR Urine Pool | Batch:2 | Analysis Order:180 RAW_FILE_NAME=VCSIP_CHEAR_U_7 SUBJECT_SAMPLE_FACTORS - VCSIP_CHEAR_U_8 Sample Type:CHEAR Urine Pool | Batch:2 | Analysis Order:207 RAW_FILE_NAME=VCSIP_CHEAR_U_8 SUBJECT_SAMPLE_FACTORS - VCSIP_CHEAR_U_9 Sample Type:CHEAR Urine Pool | Batch:3 | Analysis Order:236 RAW_FILE_NAME=VCSIP_CHEAR_U_9 SUBJECT_SAMPLE_FACTORS - VCSIP_CHEAR_U_10 Sample Type:CHEAR Urine Pool | Batch:3 | Analysis Order:264 RAW_FILE_NAME=VCSIP_CHEAR_U_10 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U1_1 Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:1 | Analysis Order:13 RAW_FILE_NAME=VCSIP_HHEAR_U1_1 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U1_2 Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:1 | Analysis Order:40 RAW_FILE_NAME=VCSIP_HHEAR_U1_2 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U1_3 Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:1 | Analysis Order:68 RAW_FILE_NAME=VCSIP_HHEAR_U1_3 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U1_4 Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:1 | Analysis Order:95 RAW_FILE_NAME=VCSIP_HHEAR_U1_4 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U1_5 Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:2 | Analysis Order:124 RAW_FILE_NAME=VCSIP_HHEAR_U1_5 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U1_6 Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:2 | Analysis Order:151 RAW_FILE_NAME=VCSIP_HHEAR_U1_6 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U1_7 Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:2 | Analysis Order:179 RAW_FILE_NAME=VCSIP_HHEAR_U1_7 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U1_8 Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:2 | Analysis Order:206 RAW_FILE_NAME=VCSIP_HHEAR_U1_8 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U1_9 Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:3 | Analysis Order:235 RAW_FILE_NAME=VCSIP_HHEAR_U1_9 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U1_10 Sample Type:HHEAR Urine Pool 1 (HHEAR_U1_20) | Batch:3 | Analysis Order:263 RAW_FILE_NAME=VCSIP_HHEAR_U1_10 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U2_4 Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:1 | Analysis Order:109 RAW_FILE_NAME=VCSIP_HHEAR_U2_4 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U2_1 Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:1 | Analysis Order:27 RAW_FILE_NAME=VCSIP_HHEAR_U2_1 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U2_2 Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:1 | Analysis Order:54 RAW_FILE_NAME=VCSIP_HHEAR_U2_2 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U2_3 Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:1 | Analysis Order:82 RAW_FILE_NAME=VCSIP_HHEAR_U2_3 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U2_5 Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:2 | Analysis Order:138 RAW_FILE_NAME=VCSIP_HHEAR_U2_5 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U2_6 Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:2 | Analysis Order:165 RAW_FILE_NAME=VCSIP_HHEAR_U2_6 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U2_7 Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:2 | Analysis Order:193 RAW_FILE_NAME=VCSIP_HHEAR_U2_7 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U2_8 Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:2 | Analysis Order:220 RAW_FILE_NAME=VCSIP_HHEAR_U2_8 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U2_9 Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:3 | Analysis Order:249 RAW_FILE_NAME=VCSIP_HHEAR_U2_9 SUBJECT_SAMPLE_FACTORS - VCSIP_HHEAR_U2_10 Sample Type:HHEAR Urine Pool 2 (HHEAR_U2_50) | Batch:3 | Analysis Order:277 RAW_FILE_NAME=VCSIP_HHEAR_U2_10 SUBJECT_SAMPLE_FACTORS - VCSIP_NIST_3672_1 Sample Type:NIST Urine Pool 3672 | Batch:1 | Analysis Order:1 RAW_FILE_NAME=VCSIP_NIST_3672_1 SUBJECT_SAMPLE_FACTORS - VCSIP_NIST_3672_3 Sample Type:NIST Urine Pool 3672 | Batch:1 | Analysis Order:111 RAW_FILE_NAME=VCSIP_NIST_3672_3 SUBJECT_SAMPLE_FACTORS - VCSIP_NIST_3672_2 Sample Type:NIST Urine Pool 3672 | Batch:1 | Analysis Order:56 RAW_FILE_NAME=VCSIP_NIST_3672_2 SUBJECT_SAMPLE_FACTORS - VCSIP_NIST_3672_4 Sample Type:NIST Urine Pool 3672 | Batch:2 | Analysis Order:112 RAW_FILE_NAME=VCSIP_NIST_3672_4 SUBJECT_SAMPLE_FACTORS - VCSIP_NIST_3672_5 Sample Type:NIST Urine Pool 3672 | Batch:2 | Analysis Order:167 RAW_FILE_NAME=VCSIP_NIST_3672_5 SUBJECT_SAMPLE_FACTORS - VCSIP_NIST_3672_6 Sample Type:NIST Urine Pool 3672 | Batch:2 | Analysis Order:222 RAW_FILE_NAME=VCSIP_NIST_3672_6 SUBJECT_SAMPLE_FACTORS - VCSIP_NIST_3672_7 Sample Type:NIST Urine Pool 3672 | Batch:3 | Analysis Order:223 RAW_FILE_NAME=VCSIP_NIST_3672_7 SUBJECT_SAMPLE_FACTORS - VCSIP_NIST_3672_8 Sample Type:NIST Urine Pool 3672 | Batch:3 | Analysis Order:251 RAW_FILE_NAME=VCSIP_NIST_3672_8 SUBJECT_SAMPLE_FACTORS - VCSIP_NIST_3672_9 Sample Type:NIST Urine Pool 3672 | Batch:3 | Analysis Order:279 RAW_FILE_NAME=VCSIP_NIST_3672_9 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_8 Sample Type:Study Pool | Batch:1 | Analysis Order:110 RAW_FILE_NAME=VCSIP_SP_8 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_1 Sample Type:Study Pool | Batch:1 | Analysis Order:15 RAW_FILE_NAME=VCSIP_SP_1 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_2 Sample Type:Study Pool | Batch:1 | Analysis Order:28 RAW_FILE_NAME=VCSIP_SP_2 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_3 Sample Type:Study Pool | Batch:1 | Analysis Order:42 RAW_FILE_NAME=VCSIP_SP_3 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_4 Sample Type:Study Pool | Batch:1 | Analysis Order:55 RAW_FILE_NAME=VCSIP_SP_4 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_5 Sample Type:Study Pool | Batch:1 | Analysis Order:70 RAW_FILE_NAME=VCSIP_SP_5 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_6 Sample Type:Study Pool | Batch:1 | Analysis Order:83 RAW_FILE_NAME=VCSIP_SP_6 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_7 Sample Type:Study Pool | Batch:1 | Analysis Order:97 RAW_FILE_NAME=VCSIP_SP_7 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_9 Sample Type:Study Pool | Batch:2 | Analysis Order:126 RAW_FILE_NAME=VCSIP_SP_9 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_10 Sample Type:Study Pool | Batch:2 | Analysis Order:139 RAW_FILE_NAME=VCSIP_SP_10 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_11 Sample Type:Study Pool | Batch:2 | Analysis Order:153 RAW_FILE_NAME=VCSIP_SP_11 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_12 Sample Type:Study Pool | Batch:2 | Analysis Order:166 RAW_FILE_NAME=VCSIP_SP_12 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_13 Sample Type:Study Pool | Batch:2 | Analysis Order:181 RAW_FILE_NAME=VCSIP_SP_13 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_14 Sample Type:Study Pool | Batch:2 | Analysis Order:194 RAW_FILE_NAME=VCSIP_SP_14 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_15 Sample Type:Study Pool | Batch:2 | Analysis Order:208 RAW_FILE_NAME=VCSIP_SP_15 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_16 Sample Type:Study Pool | Batch:2 | Analysis Order:221 RAW_FILE_NAME=VCSIP_SP_16 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_17 Sample Type:Study Pool | Batch:3 | Analysis Order:237 RAW_FILE_NAME=VCSIP_SP_17 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_18 Sample Type:Study Pool | Batch:3 | Analysis Order:250 RAW_FILE_NAME=VCSIP_SP_18 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_19 Sample Type:Study Pool | Batch:3 | Analysis Order:265 RAW_FILE_NAME=VCSIP_SP_19 SUBJECT_SAMPLE_FACTORS - VCSIP_SP_20 Sample Type:Study Pool | Batch:3 | Analysis Order:278 RAW_FILE_NAME=VCSIP_SP_20 SUBJECT_SAMPLE_FACTORS EM-6GK72-U-00-12401 VCSIP_S_9 Sample Type:Study Sample | Batch:1 | Analysis Order:10 RAW_FILE_NAME=VCSIP_S_9 SUBJECT_SAMPLE_FACTORS EM-6GN87-U-00-12401 VCSIP_S_73 Sample Type:Study Sample | Batch:1 | Analysis Order:100 RAW_FILE_NAME=VCSIP_S_73 SUBJECT_SAMPLE_FACTORS EM-5DPA2-U-00-12401 VCSIP_S_74 Sample Type:Study Sample | Batch:1 | Analysis Order:101 RAW_FILE_NAME=VCSIP_S_74 SUBJECT_SAMPLE_FACTORS EM-6G9A8-U-00-12401 VCSIP_S_75 Sample Type:Study Sample | Batch:1 | Analysis Order:102 RAW_FILE_NAME=VCSIP_S_75 SUBJECT_SAMPLE_FACTORS EM-6GDV1-U-00-12401 VCSIP_S_76 Sample Type:Study Sample | Batch:1 | Analysis Order:103 RAW_FILE_NAME=VCSIP_S_76 SUBJECT_SAMPLE_FACTORS EM-6GD61-U-00-12401 VCSIP_S_77 Sample Type:Study Sample | Batch:1 | Analysis Order:104 RAW_FILE_NAME=VCSIP_S_77 SUBJECT_SAMPLE_FACTORS EM-6GAK8-U-00-12401 VCSIP_S_78 Sample Type:Study Sample | Batch:1 | Analysis Order:105 RAW_FILE_NAME=VCSIP_S_78 SUBJECT_SAMPLE_FACTORS EM-5DFD5-U-00-12401 VCSIP_S_79 Sample Type:Study Sample | Batch:1 | Analysis Order:106 RAW_FILE_NAME=VCSIP_S_79 SUBJECT_SAMPLE_FACTORS EM-5DVN7-U-00-12401 VCSIP_S_80 Sample Type:Study Sample | Batch:1 | Analysis Order:107 RAW_FILE_NAME=VCSIP_S_80 SUBJECT_SAMPLE_FACTORS EM-5DN01-U-00-12401 VCSIP_S_10 Sample Type:Study Sample | Batch:1 | Analysis Order:11 RAW_FILE_NAME=VCSIP_S_10 SUBJECT_SAMPLE_FACTORS EM-5E1B9-U-00-12401 VCSIP_S_11 Sample Type:Study Sample | Batch:1 | Analysis Order:16 RAW_FILE_NAME=VCSIP_S_11 SUBJECT_SAMPLE_FACTORS EM-6GCM2-U-00-12401 VCSIP_S_12 Sample Type:Study Sample | Batch:1 | Analysis Order:17 RAW_FILE_NAME=VCSIP_S_12 SUBJECT_SAMPLE_FACTORS EM-5DLY3-U-00-12401 VCSIP_S_13 Sample Type:Study Sample | Batch:1 | Analysis Order:18 RAW_FILE_NAME=VCSIP_S_13 SUBJECT_SAMPLE_FACTORS EM-5DQ32-U-00-12401 VCSIP_S_14 Sample Type:Study Sample | Batch:1 | Analysis Order:19 RAW_FILE_NAME=VCSIP_S_14 SUBJECT_SAMPLE_FACTORS EM-5DN68-U-00-12401 VCSIP_S_1 Sample Type:Study Sample | Batch:1 | Analysis Order:2 RAW_FILE_NAME=VCSIP_S_1 SUBJECT_SAMPLE_FACTORS EM-6GGC0-U-00-12401 VCSIP_S_15 Sample Type:Study Sample | Batch:1 | Analysis Order:20 RAW_FILE_NAME=VCSIP_S_15 SUBJECT_SAMPLE_FACTORS EM-6G8T7-U-00-12401 VCSIP_S_16 Sample Type:Study Sample | Batch:1 | Analysis Order:21 RAW_FILE_NAME=VCSIP_S_16 SUBJECT_SAMPLE_FACTORS EM-5DZB0-U-00-12401 VCSIP_S_17 Sample Type:Study Sample | Batch:1 | Analysis Order:22 RAW_FILE_NAME=VCSIP_S_17 SUBJECT_SAMPLE_FACTORS EM-6GG27-U-00-12401 VCSIP_S_18 Sample Type:Study Sample | Batch:1 | Analysis Order:23 RAW_FILE_NAME=VCSIP_S_18 SUBJECT_SAMPLE_FACTORS EM-5E5L3-U-00-12401 VCSIP_S_19 Sample Type:Study Sample | Batch:1 | Analysis Order:24 RAW_FILE_NAME=VCSIP_S_19 SUBJECT_SAMPLE_FACTORS EM-5E314-U-00-12401 VCSIP_S_20 Sample Type:Study Sample | Batch:1 | Analysis Order:25 RAW_FILE_NAME=VCSIP_S_20 SUBJECT_SAMPLE_FACTORS EM-6GNU3-U-00-12401 VCSIP_S_21 Sample Type:Study Sample | Batch:1 | Analysis Order:29 RAW_FILE_NAME=VCSIP_S_21 SUBJECT_SAMPLE_FACTORS EM-5E4E9-U-00-12401 VCSIP_S_2 Sample Type:Study Sample | Batch:1 | Analysis Order:3 RAW_FILE_NAME=VCSIP_S_2 SUBJECT_SAMPLE_FACTORS EM-6GUW2-U-00-12401 VCSIP_S_22 Sample Type:Study Sample | Batch:1 | Analysis Order:30 RAW_FILE_NAME=VCSIP_S_22 SUBJECT_SAMPLE_FACTORS EM-5DJ13-U-00-12401 VCSIP_S_23 Sample Type:Study Sample | Batch:1 | Analysis Order:31 RAW_FILE_NAME=VCSIP_S_23 SUBJECT_SAMPLE_FACTORS EM-5DQ73-U-00-12401 VCSIP_S_24 Sample Type:Study Sample | Batch:1 | Analysis Order:32 RAW_FILE_NAME=VCSIP_S_24 SUBJECT_SAMPLE_FACTORS EM-5DH07-U-00-12401 VCSIP_S_25 Sample Type:Study Sample | Batch:1 | Analysis Order:33 RAW_FILE_NAME=VCSIP_S_25 SUBJECT_SAMPLE_FACTORS EM-5DEH8-U-00-12401 VCSIP_S_26 Sample Type:Study Sample | Batch:1 | Analysis Order:34 RAW_FILE_NAME=VCSIP_S_26 SUBJECT_SAMPLE_FACTORS EM-5DG65-U-00-12401 VCSIP_S_27 Sample Type:Study Sample | Batch:1 | Analysis Order:35 RAW_FILE_NAME=VCSIP_S_27 SUBJECT_SAMPLE_FACTORS EM-6GF85-U-00-12401 VCSIP_S_28 Sample Type:Study Sample | Batch:1 | Analysis Order:36 RAW_FILE_NAME=VCSIP_S_28 SUBJECT_SAMPLE_FACTORS EM-5E4K6-U-00-12401 VCSIP_S_29 Sample Type:Study Sample | Batch:1 | Analysis Order:37 RAW_FILE_NAME=VCSIP_S_29 SUBJECT_SAMPLE_FACTORS EM-6GN12-U-00-12401 VCSIP_S_30 Sample Type:Study Sample | Batch:1 | Analysis Order:38 RAW_FILE_NAME=VCSIP_S_30 SUBJECT_SAMPLE_FACTORS EM-6GET4-U-00-12401 VCSIP_S_3 Sample Type:Study Sample | Batch:1 | Analysis Order:4 RAW_FILE_NAME=VCSIP_S_3 SUBJECT_SAMPLE_FACTORS EM-6GEG3-U-00-12401 VCSIP_S_31 Sample Type:Study Sample | Batch:1 | Analysis Order:43 RAW_FILE_NAME=VCSIP_S_31 SUBJECT_SAMPLE_FACTORS EM-6GSU8-U-00-12401 VCSIP_S_32 Sample Type:Study Sample | Batch:1 | Analysis Order:44 RAW_FILE_NAME=VCSIP_S_32 SUBJECT_SAMPLE_FACTORS EM-6G905-U-00-12401 VCSIP_S_33 Sample Type:Study Sample | Batch:1 | Analysis Order:45 RAW_FILE_NAME=VCSIP_S_33 SUBJECT_SAMPLE_FACTORS EM-5E2V4-U-00-12401 VCSIP_S_34 Sample Type:Study Sample | Batch:1 | Analysis Order:46 RAW_FILE_NAME=VCSIP_S_34 SUBJECT_SAMPLE_FACTORS EM-6GXG4-U-00-12401 VCSIP_S_35 Sample Type:Study Sample | Batch:1 | Analysis Order:47 RAW_FILE_NAME=VCSIP_S_35 SUBJECT_SAMPLE_FACTORS EM-5DFS2-U-00-12401 VCSIP_S_36 Sample Type:Study Sample | Batch:1 | Analysis Order:48 RAW_FILE_NAME=VCSIP_S_36 SUBJECT_SAMPLE_FACTORS EM-6GDW9-U-00-12401 VCSIP_S_37 Sample Type:Study Sample | Batch:1 | Analysis Order:49 RAW_FILE_NAME=VCSIP_S_37 SUBJECT_SAMPLE_FACTORS EM-5DV11-U-00-12401 VCSIP_S_4 Sample Type:Study Sample | Batch:1 | Analysis Order:5 RAW_FILE_NAME=VCSIP_S_4 SUBJECT_SAMPLE_FACTORS EM-5E2U6-U-00-12401 VCSIP_S_38 Sample Type:Study Sample | Batch:1 | Analysis Order:50 RAW_FILE_NAME=VCSIP_S_38 SUBJECT_SAMPLE_FACTORS EM-6G8N0-U-00-12401 VCSIP_S_39 Sample Type:Study Sample | Batch:1 | Analysis Order:51 RAW_FILE_NAME=VCSIP_S_39 SUBJECT_SAMPLE_FACTORS EM-5E2Q5-U-00-12401 VCSIP_S_40 Sample Type:Study Sample | Batch:1 | Analysis Order:52 RAW_FILE_NAME=VCSIP_S_40 SUBJECT_SAMPLE_FACTORS EM-6GNE8-U-00-12401 VCSIP_S_41 Sample Type:Study Sample | Batch:1 | Analysis Order:57 RAW_FILE_NAME=VCSIP_S_41 SUBJECT_SAMPLE_FACTORS EM-5DVE7-U-00-12401 VCSIP_S_42 Sample Type:Study Sample | Batch:1 | Analysis Order:58 RAW_FILE_NAME=VCSIP_S_42 SUBJECT_SAMPLE_FACTORS EM-5DND7-U-00-12401 VCSIP_S_43 Sample Type:Study Sample | Batch:1 | Analysis Order:59 RAW_FILE_NAME=VCSIP_S_43 SUBJECT_SAMPLE_FACTORS EM-6GE03-U-00-12401 VCSIP_S_5 Sample Type:Study Sample | Batch:1 | Analysis Order:6 RAW_FILE_NAME=VCSIP_S_5 SUBJECT_SAMPLE_FACTORS EM-6GX95-U-00-12401 VCSIP_S_44 Sample Type:Study Sample | Batch:1 | Analysis Order:60 RAW_FILE_NAME=VCSIP_S_44 SUBJECT_SAMPLE_FACTORS EM-5DGP8-U-00-12401 VCSIP_S_45 Sample Type:Study Sample | Batch:1 | Analysis Order:61 RAW_FILE_NAME=VCSIP_S_45 SUBJECT_SAMPLE_FACTORS EM-5DPF1-U-00-12401 VCSIP_S_46 Sample Type:Study Sample | Batch:1 | Analysis Order:62 RAW_FILE_NAME=VCSIP_S_46 SUBJECT_SAMPLE_FACTORS EM-5E3P6-U-00-12401 VCSIP_S_47 Sample Type:Study Sample | Batch:1 | Analysis Order:63 RAW_FILE_NAME=VCSIP_S_47 SUBJECT_SAMPLE_FACTORS EM-5DEN4-U-00-12401 VCSIP_S_48 Sample Type:Study Sample | Batch:1 | Analysis Order:64 RAW_FILE_NAME=VCSIP_S_48 SUBJECT_SAMPLE_FACTORS EM-5E2J0-U-00-12401 VCSIP_S_49 Sample Type:Study Sample | Batch:1 | Analysis Order:65 RAW_FILE_NAME=VCSIP_S_49 SUBJECT_SAMPLE_FACTORS EM-5DGU7-U-00-12401 VCSIP_S_50 Sample Type:Study Sample | Batch:1 | Analysis Order:66 RAW_FILE_NAME=VCSIP_S_50 SUBJECT_SAMPLE_FACTORS EM-6GME9-U-00-12401 VCSIP_S_6 Sample Type:Study Sample | Batch:1 | Analysis Order:7 RAW_FILE_NAME=VCSIP_S_6 SUBJECT_SAMPLE_FACTORS EM-5E751-U-00-12401 VCSIP_S_51 Sample Type:Study Sample | Batch:1 | Analysis Order:71 RAW_FILE_NAME=VCSIP_S_51 SUBJECT_SAMPLE_FACTORS EM-6GKQ5-U-00-12401 VCSIP_S_52 Sample Type:Study Sample | Batch:1 | Analysis Order:72 RAW_FILE_NAME=VCSIP_S_52 SUBJECT_SAMPLE_FACTORS EM-6GPJ5-U-00-12401 VCSIP_S_53 Sample Type:Study Sample | Batch:1 | Analysis Order:73 RAW_FILE_NAME=VCSIP_S_53 SUBJECT_SAMPLE_FACTORS EM-5DLX5-U-00-12401 VCSIP_S_54 Sample Type:Study Sample | Batch:1 | Analysis Order:74 RAW_FILE_NAME=VCSIP_S_54 SUBJECT_SAMPLE_FACTORS EM-6GQU0-U-00-12401 VCSIP_S_55 Sample Type:Study Sample | Batch:1 | Analysis Order:75 RAW_FILE_NAME=VCSIP_S_55 SUBJECT_SAMPLE_FACTORS EM-5DFJ2-U-00-12401 VCSIP_S_56 Sample Type:Study Sample | Batch:1 | Analysis Order:76 RAW_FILE_NAME=VCSIP_S_56 SUBJECT_SAMPLE_FACTORS EM-5E512-U-00-12401 VCSIP_S_57 Sample Type:Study Sample | Batch:1 | Analysis Order:77 RAW_FILE_NAME=VCSIP_S_57 SUBJECT_SAMPLE_FACTORS EM-5DRV4-U-00-12401 VCSIP_S_58 Sample Type:Study Sample | Batch:1 | Analysis Order:78 RAW_FILE_NAME=VCSIP_S_58 SUBJECT_SAMPLE_FACTORS EM-6GVJ9-U-00-12401 VCSIP_S_59 Sample Type:Study Sample | Batch:1 | Analysis Order:79 RAW_FILE_NAME=VCSIP_S_59 SUBJECT_SAMPLE_FACTORS EM-6GUQ5-U-00-12401 VCSIP_S_7 Sample Type:Study Sample | Batch:1 | Analysis Order:8 RAW_FILE_NAME=VCSIP_S_7 SUBJECT_SAMPLE_FACTORS EM-5E0H7-U-00-12401 VCSIP_S_60 Sample Type:Study Sample | Batch:1 | Analysis Order:80 RAW_FILE_NAME=VCSIP_S_60 SUBJECT_SAMPLE_FACTORS EM-6GRT1-U-00-12401 VCSIP_S_61 Sample Type:Study Sample | Batch:1 | Analysis Order:84 RAW_FILE_NAME=VCSIP_S_61 SUBJECT_SAMPLE_FACTORS EM-5E6L2-U-00-12401 VCSIP_S_62 Sample Type:Study Sample | Batch:1 | Analysis Order:85 RAW_FILE_NAME=VCSIP_S_62 SUBJECT_SAMPLE_FACTORS EM-6GJW3-U-00-12401 VCSIP_S_63 Sample Type:Study Sample | Batch:1 | Analysis Order:86 RAW_FILE_NAME=VCSIP_S_63 SUBJECT_SAMPLE_FACTORS EM-5DU79-U-00-12401 VCSIP_S_64 Sample Type:Study Sample | Batch:1 | Analysis Order:87 RAW_FILE_NAME=VCSIP_S_64 SUBJECT_SAMPLE_FACTORS EM-5DZU8-U-00-12401 VCSIP_S_65 Sample Type:Study Sample | Batch:1 | Analysis Order:88 RAW_FILE_NAME=VCSIP_S_65 SUBJECT_SAMPLE_FACTORS EM-6GBL5-U-00-12401 VCSIP_S_66 Sample Type:Study Sample | Batch:1 | Analysis Order:89 RAW_FILE_NAME=VCSIP_S_66 SUBJECT_SAMPLE_FACTORS EM-6GRA3-U-00-12401 VCSIP_S_8 Sample Type:Study Sample | Batch:1 | Analysis Order:9 RAW_FILE_NAME=VCSIP_S_8 SUBJECT_SAMPLE_FACTORS EM-5DU12-U-00-12401 VCSIP_S_67 Sample Type:Study Sample | Batch:1 | Analysis Order:90 RAW_FILE_NAME=VCSIP_S_67 SUBJECT_SAMPLE_FACTORS EM-6GHN4-U-00-12401 VCSIP_S_68 Sample Type:Study Sample | Batch:1 | Analysis Order:91 RAW_FILE_NAME=VCSIP_S_68 SUBJECT_SAMPLE_FACTORS EM-6GFB3-U-00-12401 VCSIP_S_69 Sample Type:Study Sample | Batch:1 | Analysis Order:92 RAW_FILE_NAME=VCSIP_S_69 SUBJECT_SAMPLE_FACTORS EM-5DKY4-U-00-12401 VCSIP_S_70 Sample Type:Study Sample | Batch:1 | Analysis Order:93 RAW_FILE_NAME=VCSIP_S_70 SUBJECT_SAMPLE_FACTORS EM-6GQQ9-U-00-12401 VCSIP_S_71 Sample Type:Study Sample | Batch:1 | Analysis Order:98 RAW_FILE_NAME=VCSIP_S_71 SUBJECT_SAMPLE_FACTORS EM-5DXW6-U-00-12401 VCSIP_S_72 Sample Type:Study Sample | Batch:1 | Analysis Order:99 RAW_FILE_NAME=VCSIP_S_72 SUBJECT_SAMPLE_FACTORS EM-6GND0-U-00-12401 VCSIP_S_81 Sample Type:Study Sample | Batch:2 | Analysis Order:113 RAW_FILE_NAME=VCSIP_S_81 SUBJECT_SAMPLE_FACTORS EM-5DQB9-U-00-12401 VCSIP_S_82 Sample Type:Study Sample | Batch:2 | Analysis Order:114 RAW_FILE_NAME=VCSIP_S_82 SUBJECT_SAMPLE_FACTORS EM-5DRQ5-U-00-12401 VCSIP_S_83 Sample Type:Study Sample | Batch:2 | Analysis Order:115 RAW_FILE_NAME=VCSIP_S_83 SUBJECT_SAMPLE_FACTORS EM-6G970-U-00-12401 VCSIP_S_84 Sample Type:Study Sample | Batch:2 | Analysis Order:116 RAW_FILE_NAME=VCSIP_S_84 SUBJECT_SAMPLE_FACTORS EM-6GG68-U-00-12401 VCSIP_S_85 Sample Type:Study Sample | Batch:2 | Analysis Order:117 RAW_FILE_NAME=VCSIP_S_85 SUBJECT_SAMPLE_FACTORS EM-6GLF8-U-00-12401 VCSIP_S_86 Sample Type:Study Sample | Batch:2 | Analysis Order:118 RAW_FILE_NAME=VCSIP_S_86 SUBJECT_SAMPLE_FACTORS EM-6GCP5-U-00-12401 VCSIP_S_87 Sample Type:Study Sample | Batch:2 | Analysis Order:119 RAW_FILE_NAME=VCSIP_S_87 SUBJECT_SAMPLE_FACTORS EM-5DQQ6-U-00-12401 VCSIP_S_88 Sample Type:Study Sample | Batch:2 | Analysis Order:120 RAW_FILE_NAME=VCSIP_S_88 SUBJECT_SAMPLE_FACTORS EM-5DUD0-U-00-12401 VCSIP_S_89 Sample Type:Study Sample | Batch:2 | Analysis Order:121 RAW_FILE_NAME=VCSIP_S_89 SUBJECT_SAMPLE_FACTORS EM-6GTD4-U-00-12401 VCSIP_S_90 Sample Type:Study Sample | Batch:2 | Analysis Order:122 RAW_FILE_NAME=VCSIP_S_90 SUBJECT_SAMPLE_FACTORS EM-6GE52-U-00-12401 VCSIP_S_91 Sample Type:Study Sample | Batch:2 | Analysis Order:127 RAW_FILE_NAME=VCSIP_S_91 SUBJECT_SAMPLE_FACTORS EM-6GPV9-U-00-12401 VCSIP_S_92 Sample Type:Study Sample | Batch:2 | Analysis Order:128 RAW_FILE_NAME=VCSIP_S_92 SUBJECT_SAMPLE_FACTORS EM-6GQC0-U-00-12401 VCSIP_S_93 Sample Type:Study Sample | Batch:2 | Analysis Order:129 RAW_FILE_NAME=VCSIP_S_93 SUBJECT_SAMPLE_FACTORS EM-5DGE2-U-00-12401 VCSIP_S_94 Sample Type:Study Sample | Batch:2 | Analysis Order:130 RAW_FILE_NAME=VCSIP_S_94 SUBJECT_SAMPLE_FACTORS EM-6GMV2-U-00-12401 VCSIP_S_95 Sample Type:Study Sample | Batch:2 | Analysis Order:131 RAW_FILE_NAME=VCSIP_S_95 SUBJECT_SAMPLE_FACTORS EM-6GHB1-U-00-12401 VCSIP_S_96 Sample Type:Study Sample | Batch:2 | Analysis Order:132 RAW_FILE_NAME=VCSIP_S_96 SUBJECT_SAMPLE_FACTORS EM-6GLV3-U-00-12401 VCSIP_S_97 Sample Type:Study Sample | Batch:2 | Analysis Order:133 RAW_FILE_NAME=VCSIP_S_97 SUBJECT_SAMPLE_FACTORS EM-5E6H1-U-00-12401 VCSIP_S_98 Sample Type:Study Sample | Batch:2 | Analysis Order:134 RAW_FILE_NAME=VCSIP_S_98 SUBJECT_SAMPLE_FACTORS EM-6GSP9-U-00-12401 VCSIP_S_99 Sample Type:Study Sample | Batch:2 | Analysis Order:135 RAW_FILE_NAME=VCSIP_S_99 SUBJECT_SAMPLE_FACTORS EM-5DXT2-U-00-12401 VCSIP_S_100 Sample Type:Study Sample | Batch:2 | Analysis Order:136 RAW_FILE_NAME=VCSIP_S_100 SUBJECT_SAMPLE_FACTORS EM-6GEJ6-U-00-12401 VCSIP_S_101 Sample Type:Study Sample | Batch:2 | Analysis Order:140 RAW_FILE_NAME=VCSIP_S_101 SUBJECT_SAMPLE_FACTORS EM-5E439-U-00-12401 VCSIP_S_102 Sample Type:Study Sample | Batch:2 | Analysis Order:141 RAW_FILE_NAME=VCSIP_S_102 SUBJECT_SAMPLE_FACTORS EM-6GUV4-U-00-12401 VCSIP_S_103 Sample Type:Study Sample | Batch:2 | Analysis Order:142 RAW_FILE_NAME=VCSIP_S_103 SUBJECT_SAMPLE_FACTORS EM-5DV37-U-00-12401 VCSIP_S_104 Sample Type:Study Sample | Batch:2 | Analysis Order:143 RAW_FILE_NAME=VCSIP_S_104 SUBJECT_SAMPLE_FACTORS EM-6GPQ0-U-00-12401 VCSIP_S_105 Sample Type:Study Sample | Batch:2 | Analysis Order:144 RAW_FILE_NAME=VCSIP_S_105 SUBJECT_SAMPLE_FACTORS EM-6GWM2-U-00-12401 VCSIP_S_106 Sample Type:Study Sample | Batch:2 | Analysis Order:145 RAW_FILE_NAME=VCSIP_S_106 SUBJECT_SAMPLE_FACTORS EM-5DYA3-U-00-12401 VCSIP_S_107 Sample Type:Study Sample | Batch:2 | Analysis Order:146 RAW_FILE_NAME=VCSIP_S_107 SUBJECT_SAMPLE_FACTORS EM-6GQY1-U-00-12401 VCSIP_S_108 Sample Type:Study Sample | Batch:2 | Analysis Order:147 RAW_FILE_NAME=VCSIP_S_108 SUBJECT_SAMPLE_FACTORS EM-6GBP6-U-00-12401 VCSIP_S_109 Sample Type:Study Sample | Batch:2 | Analysis Order:148 RAW_FILE_NAME=VCSIP_S_109 SUBJECT_SAMPLE_FACTORS EM-6GPK3-U-00-12401 VCSIP_S_110 Sample Type:Study Sample | Batch:2 | Analysis Order:149 RAW_FILE_NAME=VCSIP_S_110 SUBJECT_SAMPLE_FACTORS EM-6G889-U-00-12401 VCSIP_S_111 Sample Type:Study Sample | Batch:2 | Analysis Order:154 RAW_FILE_NAME=VCSIP_S_111 SUBJECT_SAMPLE_FACTORS EM-6GMN9-U-00-12401 VCSIP_S_112 Sample Type:Study Sample | Batch:2 | Analysis Order:155 RAW_FILE_NAME=VCSIP_S_112 SUBJECT_SAMPLE_FACTORS EM-6GHD6-U-00-12401 VCSIP_S_113 Sample Type:Study Sample | Batch:2 | Analysis Order:156 RAW_FILE_NAME=VCSIP_S_113 SUBJECT_SAMPLE_FACTORS EM-5E4S8-U-00-12401 VCSIP_S_114 Sample Type:Study Sample | Batch:2 | Analysis Order:157 RAW_FILE_NAME=VCSIP_S_114 SUBJECT_SAMPLE_FACTORS EM-5DP25-U-00-12401 VCSIP_S_115 Sample Type:Study Sample | Batch:2 | Analysis Order:158 RAW_FILE_NAME=VCSIP_S_115 SUBJECT_SAMPLE_FACTORS EM-5DHN1-U-00-12401 VCSIP_S_116 Sample Type:Study Sample | Batch:2 | Analysis Order:159 RAW_FILE_NAME=VCSIP_S_116 SUBJECT_SAMPLE_FACTORS EM-6GTU7-U-00-12401 VCSIP_S_117 Sample Type:Study Sample | Batch:2 | Analysis Order:160 RAW_FILE_NAME=VCSIP_S_117 SUBJECT_SAMPLE_FACTORS EM-5E2L6-U-00-12401 VCSIP_S_118 Sample Type:Study Sample | Batch:2 | Analysis Order:161 RAW_FILE_NAME=VCSIP_S_118 SUBJECT_SAMPLE_FACTORS EM-5DTG5-U-00-12401 VCSIP_S_119 Sample Type:Study Sample | Batch:2 | Analysis Order:162 RAW_FILE_NAME=VCSIP_S_119 SUBJECT_SAMPLE_FACTORS EM-6GD46-U-00-12401 VCSIP_S_120 Sample Type:Study Sample | Batch:2 | Analysis Order:163 RAW_FILE_NAME=VCSIP_S_120 SUBJECT_SAMPLE_FACTORS EM-5DW10-U-00-12401 VCSIP_S_121 Sample Type:Study Sample | Batch:2 | Analysis Order:168 RAW_FILE_NAME=VCSIP_S_121 SUBJECT_SAMPLE_FACTORS EM-6GF36-U-00-12401 VCSIP_S_122 Sample Type:Study Sample | Batch:2 | Analysis Order:169 RAW_FILE_NAME=VCSIP_S_122 SUBJECT_SAMPLE_FACTORS EM-5DQA1-U-00-12401 VCSIP_S_123 Sample Type:Study Sample | Batch:2 | Analysis Order:170 RAW_FILE_NAME=VCSIP_S_123 SUBJECT_SAMPLE_FACTORS EM-6GWP5-U-00-12401 VCSIP_S_124 Sample Type:Study Sample | Batch:2 | Analysis Order:171 RAW_FILE_NAME=VCSIP_S_124 SUBJECT_SAMPLE_FACTORS EM-5DY42-U-00-12401 VCSIP_S_125 Sample Type:Study Sample | Batch:2 | Analysis Order:172 RAW_FILE_NAME=VCSIP_S_125 SUBJECT_SAMPLE_FACTORS EM-6GL71-U-00-12401 VCSIP_S_126 Sample Type:Study Sample | Batch:2 | Analysis Order:173 RAW_FILE_NAME=VCSIP_S_126 SUBJECT_SAMPLE_FACTORS EM-5E4V2-U-00-12401 VCSIP_S_127 Sample Type:Study Sample | Batch:2 | Analysis Order:174 RAW_FILE_NAME=VCSIP_S_127 SUBJECT_SAMPLE_FACTORS EM-6GVX8-U-00-12401 VCSIP_S_128 Sample Type:Study Sample | Batch:2 | Analysis Order:175 RAW_FILE_NAME=VCSIP_S_128 SUBJECT_SAMPLE_FACTORS EM-5E6V0-U-00-12401 VCSIP_S_129 Sample Type:Study Sample | Batch:2 | Analysis Order:176 RAW_FILE_NAME=VCSIP_S_129 SUBJECT_SAMPLE_FACTORS EM-5DNC0-U-00-12401 VCSIP_S_130 Sample Type:Study Sample | Batch:2 | Analysis Order:177 RAW_FILE_NAME=VCSIP_S_130 SUBJECT_SAMPLE_FACTORS EM-5DJX7-U-00-12401 VCSIP_S_131 Sample Type:Study Sample | Batch:2 | Analysis Order:182 RAW_FILE_NAME=VCSIP_S_131 SUBJECT_SAMPLE_FACTORS EM-5DD50-U-00-12401 VCSIP_S_132 Sample Type:Study Sample | Batch:2 | Analysis Order:183 RAW_FILE_NAME=VCSIP_S_132 SUBJECT_SAMPLE_FACTORS EM-6GJF0-U-00-12401 VCSIP_S_133 Sample Type:Study Sample | Batch:2 | Analysis Order:184 RAW_FILE_NAME=VCSIP_S_133 SUBJECT_SAMPLE_FACTORS EM-6GEB4-U-00-12401 VCSIP_S_134 Sample Type:Study Sample | Batch:2 | Analysis Order:185 RAW_FILE_NAME=VCSIP_S_134 SUBJECT_SAMPLE_FACTORS EM-6GQS4-U-00-12401 VCSIP_S_135 Sample Type:Study Sample | Batch:2 | Analysis Order:186 RAW_FILE_NAME=VCSIP_S_135 SUBJECT_SAMPLE_FACTORS EM-5E3S9-U-00-12401 VCSIP_S_136 Sample Type:Study Sample | Batch:2 | Analysis Order:187 RAW_FILE_NAME=VCSIP_S_136 SUBJECT_SAMPLE_FACTORS EM-5DFH7-U-00-12401 VCSIP_S_137 Sample Type:Study Sample | Batch:2 | Analysis Order:188 RAW_FILE_NAME=VCSIP_S_137 SUBJECT_SAMPLE_FACTORS EM-5DWL1-U-00-12401 VCSIP_S_138 Sample Type:Study Sample | Batch:2 | Analysis Order:189 RAW_FILE_NAME=VCSIP_S_138 SUBJECT_SAMPLE_FACTORS EM-5DZ25-U-00-12401 VCSIP_S_139 Sample Type:Study Sample | Batch:2 | Analysis Order:190 RAW_FILE_NAME=VCSIP_S_139 SUBJECT_SAMPLE_FACTORS EM-6G9P5-U-00-12401 VCSIP_S_140 Sample Type:Study Sample | Batch:2 | Analysis Order:191 RAW_FILE_NAME=VCSIP_S_140 SUBJECT_SAMPLE_FACTORS EM-6GJ81-U-00-12401 VCSIP_S_141 Sample Type:Study Sample | Batch:2 | Analysis Order:195 RAW_FILE_NAME=VCSIP_S_141 SUBJECT_SAMPLE_FACTORS EM-6GP69-U-00-12401 VCSIP_S_142 Sample Type:Study Sample | Batch:2 | Analysis Order:196 RAW_FILE_NAME=VCSIP_S_142 SUBJECT_SAMPLE_FACTORS EM-6GDT5-U-00-12401 VCSIP_S_143 Sample Type:Study Sample | Batch:2 | Analysis Order:197 RAW_FILE_NAME=VCSIP_S_143 SUBJECT_SAMPLE_FACTORS EM-5DVM0-U-00-12401 VCSIP_S_144 Sample Type:Study Sample | Batch:2 | Analysis Order:198 RAW_FILE_NAME=VCSIP_S_144 SUBJECT_SAMPLE_FACTORS EM-6GN61-U-00-12401 VCSIP_S_145 Sample Type:Study Sample | Batch:2 | Analysis Order:199 RAW_FILE_NAME=VCSIP_S_145 SUBJECT_SAMPLE_FACTORS EM-5DTK6-U-00-12401 VCSIP_S_146 Sample Type:Study Sample | Batch:2 | Analysis Order:200 RAW_FILE_NAME=VCSIP_S_146 SUBJECT_SAMPLE_FACTORS EM-5DRN1-U-00-12401 VCSIP_S_147 Sample Type:Study Sample | Batch:2 | Analysis Order:201 RAW_FILE_NAME=VCSIP_S_147 SUBJECT_SAMPLE_FACTORS EM-5E330-U-00-12401 VCSIP_S_148 Sample Type:Study Sample | Batch:2 | Analysis Order:202 RAW_FILE_NAME=VCSIP_S_148 SUBJECT_SAMPLE_FACTORS EM-5DWC1-U-00-12401 VCSIP_S_149 Sample Type:Study Sample | Batch:2 | Analysis Order:203 RAW_FILE_NAME=VCSIP_S_149 SUBJECT_SAMPLE_FACTORS EM-5DPB0-U-00-12401 VCSIP_S_150 Sample Type:Study Sample | Batch:2 | Analysis Order:204 RAW_FILE_NAME=VCSIP_S_150 SUBJECT_SAMPLE_FACTORS EM-5DPL8-U-00-12401 VCSIP_S_151 Sample Type:Study Sample | Batch:2 | Analysis Order:209 RAW_FILE_NAME=VCSIP_S_151 SUBJECT_SAMPLE_FACTORS EM-6GNR0-U-00-12401 VCSIP_S_152 Sample Type:Study Sample | Batch:2 | Analysis Order:210 RAW_FILE_NAME=VCSIP_S_152 SUBJECT_SAMPLE_FACTORS EM-6GRB1-U-00-12401 VCSIP_S_153 Sample Type:Study Sample | Batch:2 | Analysis Order:211 RAW_FILE_NAME=VCSIP_S_153 SUBJECT_SAMPLE_FACTORS EM-6GUF9-U-00-12401 VCSIP_S_154 Sample Type:Study Sample | Batch:2 | Analysis Order:212 RAW_FILE_NAME=VCSIP_S_154 SUBJECT_SAMPLE_FACTORS EM-6GUY7-U-00-12401 VCSIP_S_155 Sample Type:Study Sample | Batch:2 | Analysis Order:213 RAW_FILE_NAME=VCSIP_S_155 SUBJECT_SAMPLE_FACTORS EM-5DMU1-U-00-12401 VCSIP_S_156 Sample Type:Study Sample | Batch:2 | Analysis Order:214 RAW_FILE_NAME=VCSIP_S_156 SUBJECT_SAMPLE_FACTORS EM-5DTP5-U-00-12401 VCSIP_S_157 Sample Type:Study Sample | Batch:2 | Analysis Order:215 RAW_FILE_NAME=VCSIP_S_157 SUBJECT_SAMPLE_FACTORS EM-5DQ99-U-00-12401 VCSIP_S_158 Sample Type:Study Sample | Batch:2 | Analysis Order:216 RAW_FILE_NAME=VCSIP_S_158 SUBJECT_SAMPLE_FACTORS EM-5DKK5-U-00-12401 VCSIP_S_159 Sample Type:Study Sample | Batch:2 | Analysis Order:217 RAW_FILE_NAME=VCSIP_S_159 SUBJECT_SAMPLE_FACTORS EM-5DEG0-U-00-12401 VCSIP_S_160 Sample Type:Study Sample | Batch:2 | Analysis Order:218 RAW_FILE_NAME=VCSIP_S_160 SUBJECT_SAMPLE_FACTORS EM-5DZ33-U-00-12401 VCSIP_S_161 Sample Type:Study Sample | Batch:3 | Analysis Order:224 RAW_FILE_NAME=VCSIP_S_161 SUBJECT_SAMPLE_FACTORS EM-6GLQ4-U-00-12401 VCSIP_S_162 Sample Type:Study Sample | Batch:3 | Analysis Order:225 RAW_FILE_NAME=VCSIP_S_162 SUBJECT_SAMPLE_FACTORS EM-5DNP1-U-00-12401 VCSIP_S_163 Sample Type:Study Sample | Batch:3 | Analysis Order:226 RAW_FILE_NAME=VCSIP_S_163 SUBJECT_SAMPLE_FACTORS EM-6G8M3-U-00-12401 VCSIP_S_164 Sample Type:Study Sample | Batch:3 | Analysis Order:227 RAW_FILE_NAME=VCSIP_S_164 SUBJECT_SAMPLE_FACTORS EM-5E5Q2-U-00-12401 VCSIP_S_165 Sample Type:Study Sample | Batch:3 | Analysis Order:228 RAW_FILE_NAME=VCSIP_S_165 SUBJECT_SAMPLE_FACTORS EM-6GM39-U-00-12401 VCSIP_S_166 Sample Type:Study Sample | Batch:3 | Analysis Order:229 RAW_FILE_NAME=VCSIP_S_166 SUBJECT_SAMPLE_FACTORS EM-5E520-U-00-12401 VCSIP_S_167 Sample Type:Study Sample | Batch:3 | Analysis Order:230 RAW_FILE_NAME=VCSIP_S_167 SUBJECT_SAMPLE_FACTORS EM-5DLM0-U-00-12401 VCSIP_S_168 Sample Type:Study Sample | Batch:3 | Analysis Order:231 RAW_FILE_NAME=VCSIP_S_168 SUBJECT_SAMPLE_FACTORS EM-5DGM5-U-00-12401 VCSIP_S_169 Sample Type:Study Sample | Batch:3 | Analysis Order:232 RAW_FILE_NAME=VCSIP_S_169 SUBJECT_SAMPLE_FACTORS EM-5DF58-U-00-12401 VCSIP_S_170 Sample Type:Study Sample | Batch:3 | Analysis Order:233 RAW_FILE_NAME=VCSIP_S_170 SUBJECT_SAMPLE_FACTORS EM-5DZT0-U-00-12401 VCSIP_S_171 Sample Type:Study Sample | Batch:3 | Analysis Order:238 RAW_FILE_NAME=VCSIP_S_171 SUBJECT_SAMPLE_FACTORS EM-5DE91-U-00-12401 VCSIP_S_172 Sample Type:Study Sample | Batch:3 | Analysis Order:239 RAW_FILE_NAME=VCSIP_S_172 SUBJECT_SAMPLE_FACTORS EM-5DDH9-U-00-12401 VCSIP_S_173 Sample Type:Study Sample | Batch:3 | Analysis Order:240 RAW_FILE_NAME=VCSIP_S_173 SUBJECT_SAMPLE_FACTORS EM-5E4J8-U-00-12401 VCSIP_S_174 Sample Type:Study Sample | Batch:3 | Analysis Order:241 RAW_FILE_NAME=VCSIP_S_174 SUBJECT_SAMPLE_FACTORS EM-5DL52-U-00-12401 VCSIP_S_175 Sample Type:Study Sample | Batch:3 | Analysis Order:242 RAW_FILE_NAME=VCSIP_S_175 SUBJECT_SAMPLE_FACTORS EM-6GUG7-U-00-12401 VCSIP_S_176 Sample Type:Study Sample | Batch:3 | Analysis Order:243 RAW_FILE_NAME=VCSIP_S_176 SUBJECT_SAMPLE_FACTORS EM-5E1T9-U-00-12401 VCSIP_S_177 Sample Type:Study Sample | Batch:3 | Analysis Order:244 RAW_FILE_NAME=VCSIP_S_177 SUBJECT_SAMPLE_FACTORS EM-5DTD1-U-00-12401 VCSIP_S_178 Sample Type:Study Sample | Batch:3 | Analysis Order:245 RAW_FILE_NAME=VCSIP_S_178 SUBJECT_SAMPLE_FACTORS EM-6GWT6-U-00-12401 VCSIP_S_179 Sample Type:Study Sample | Batch:3 | Analysis Order:246 RAW_FILE_NAME=VCSIP_S_179 SUBJECT_SAMPLE_FACTORS EM-5DDA4-U-00-12401 VCSIP_S_180 Sample Type:Study Sample | Batch:3 | Analysis Order:247 RAW_FILE_NAME=VCSIP_S_180 SUBJECT_SAMPLE_FACTORS EM-6GGD7-U-00-12401 VCSIP_S_181 Sample Type:Study Sample | Batch:3 | Analysis Order:252 RAW_FILE_NAME=VCSIP_S_181 SUBJECT_SAMPLE_FACTORS EM-6G897-U-00-12401 VCSIP_S_182 Sample Type:Study Sample | Batch:3 | Analysis Order:253 RAW_FILE_NAME=VCSIP_S_182 SUBJECT_SAMPLE_FACTORS EM-5DSH4-U-00-12401 VCSIP_S_183 Sample Type:Study Sample | Batch:3 | Analysis Order:254 RAW_FILE_NAME=VCSIP_S_183 SUBJECT_SAMPLE_FACTORS EM-5DDT2-U-00-12401 VCSIP_S_184 Sample Type:Study Sample | Batch:3 | Analysis Order:255 RAW_FILE_NAME=VCSIP_S_184 SUBJECT_SAMPLE_FACTORS EM-6GBR2-U-00-12401 VCSIP_S_185 Sample Type:Study Sample | Batch:3 | Analysis Order:256 RAW_FILE_NAME=VCSIP_S_185 SUBJECT_SAMPLE_FACTORS EM-5E1C7-U-00-12401 VCSIP_S_186 Sample Type:Study Sample | Batch:3 | Analysis Order:257 RAW_FILE_NAME=VCSIP_S_186 SUBJECT_SAMPLE_FACTORS EM-6G9M2-U-00-12401 VCSIP_S_187 Sample Type:Study Sample | Batch:3 | Analysis Order:258 RAW_FILE_NAME=VCSIP_S_187 SUBJECT_SAMPLE_FACTORS EM-6GS09-U-00-12401 VCSIP_S_188 Sample Type:Study Sample | Batch:3 | Analysis Order:259 RAW_FILE_NAME=VCSIP_S_188 SUBJECT_SAMPLE_FACTORS EM-5DP33-U-00-12401 VCSIP_S_189 Sample Type:Study Sample | Batch:3 | Analysis Order:260 RAW_FILE_NAME=VCSIP_S_189 SUBJECT_SAMPLE_FACTORS EM-6GP85-U-00-12401 VCSIP_S_190 Sample Type:Study Sample | Batch:3 | Analysis Order:261 RAW_FILE_NAME=VCSIP_S_190 SUBJECT_SAMPLE_FACTORS EM-5DZ58-U-00-12401 VCSIP_S_191 Sample Type:Study Sample | Batch:3 | Analysis Order:266 RAW_FILE_NAME=VCSIP_S_191 SUBJECT_SAMPLE_FACTORS EM-5DF09-U-00-12401 VCSIP_S_192 Sample Type:Study Sample | Batch:3 | Analysis Order:267 RAW_FILE_NAME=VCSIP_S_192 SUBJECT_SAMPLE_FACTORS EM-6GNX6-U-00-12401 VCSIP_S_193 Sample Type:Study Sample | Batch:3 | Analysis Order:268 RAW_FILE_NAME=VCSIP_S_193 SUBJECT_SAMPLE_FACTORS EM-6GBA9-U-00-12401 VCSIP_S_194 Sample Type:Study Sample | Batch:3 | Analysis Order:269 RAW_FILE_NAME=VCSIP_S_194 SUBJECT_SAMPLE_FACTORS EM-5DF66-U-00-12401 VCSIP_S_195 Sample Type:Study Sample | Batch:3 | Analysis Order:270 RAW_FILE_NAME=VCSIP_S_195 SUBJECT_SAMPLE_FACTORS EM-5DW77-U-00-12401 VCSIP_S_196 Sample Type:Study Sample | Batch:3 | Analysis Order:271 RAW_FILE_NAME=VCSIP_S_196 SUBJECT_SAMPLE_FACTORS EM-5DN19-U-00-12401 VCSIP_S_197 Sample Type:Study Sample | Batch:3 | Analysis Order:272 RAW_FILE_NAME=VCSIP_S_197 SUBJECT_SAMPLE_FACTORS EM-5DSA9-U-00-12401 VCSIP_S_198 Sample Type:Study Sample | Batch:3 | Analysis Order:273 RAW_FILE_NAME=VCSIP_S_198 SUBJECT_SAMPLE_FACTORS EM-6G9Y5-U-00-12401 VCSIP_S_199 Sample Type:Study Sample | Batch:3 | Analysis Order:274 RAW_FILE_NAME=VCSIP_S_199 SUBJECT_SAMPLE_FACTORS EM-6GFW7-U-00-12401 VCSIP_S_200 Sample Type:Study Sample | Batch:3 | Analysis Order:275 RAW_FILE_NAME=VCSIP_S_200 #COLLECTION CO:COLLECTION_SUMMARY N/A CO:SAMPLE_TYPE Urine CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY NA #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Study samples were shipped from the Oregon Health and Sciences University to the SP:SAMPLEPREP_SUMMARY NC HHEAR Hub on dry ice. The NC HHEAR Hub thawed and transferred 50 µL of the SP:SAMPLEPREP_SUMMARY study samples to a new set of tubes and used them for the analysis. An SP:SAMPLEPREP_SUMMARY additional 10 µL was taken from the original study sample and transferred to SP:SAMPLEPREP_SUMMARY another tube to make the total study pool for this project, and then distributed SP:SAMPLEPREP_SUMMARY with 50 µL per aliquot, used as quality control study pools (QC study pools) SP:SAMPLEPREP_SUMMARY throughout the whole analysis. All sample aliquots (50 µL each) and QC study SP:SAMPLEPREP_SUMMARY pools (50 µL each) were stored at -80° C until the day of sample preparation. SP:SAMPLEPREP_SUMMARY HHEAR Urine Pool 1 (50 µL each), HHEAR Urine Pool 2 (50 µL each), CHEAR SP:SAMPLEPREP_SUMMARY reference urine (50 µL each) and NIST urine (SRM 3672) reference material (50 SP:SAMPLEPREP_SUMMARY µL each) were provided by the NC HHEAR Hub. LC-MS grade water (50 µL) was used SP:SAMPLEPREP_SUMMARY as blanks. All samples were thawed at 4°C overnight before the preparation. SP:SAMPLEPREP_SUMMARY Samples, including study samples, study pool samples, HHEAR reference urine, SP:SAMPLEPREP_SUMMARY CHEAR reference urine, NIST reference urine, and blanks were mixed with 400 µL SP:SAMPLEPREP_SUMMARY methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by SP:SAMPLEPREP_SUMMARY a multiple tube vortex mixer for 2 min at 5000 rpm at room temperature. All SP:SAMPLEPREP_SUMMARY samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 SP:SAMPLEPREP_SUMMARY µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, dried by SP:SAMPLEPREP_SUMMARY a SpeedVac overnight, and stored at -80° C. For immediate analysis, 100 µL of SP:SAMPLEPREP_SUMMARY water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts. SP:SAMPLEPREP_SUMMARY Samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 SP:SAMPLEPREP_SUMMARY rpm at room temperature and then centrifuged at 4°C for 10 min at 16,000 rcf. SP:SAMPLEPREP_SUMMARY The supernatant was transferred to pre-labeled autosampler vials for data SP:SAMPLEPREP_SUMMARY acquisition by LC-MS. SP:PROCESSING_STORAGE_CONDITIONS 4℃ SP:EXTRACTION_METHOD Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard SP:EXTRACT_STORAGE -80℃ SP:SAMPLE_RESUSPENSION Water-Methanol (95:5, v/v) SP:SAMPLE_SPIKING Tryptophan-d5 stock solution at 500 ng/mL #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Reverse phase CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) CH:COLUMN_PRESSURE 6000-10000 psi CH:COLUMN_TEMPERATURE 50℃ CH:FLOW_GRADIENT Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. CH:FLOW_GRADIENT 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 CH:FLOW_GRADIENT 0.4 99.0 1.0 5 CH:FLOW_RATE 0.4 mL/min CH:INJECTION_TEMPERATURE 8oC CH:INTERNAL_STANDARD Tryptophan-d5 CH:SOLVENT_A 100% water; 0.1% formic acid CH:SOLVENT_B 100% methanol; 0.1% formic acid CH:ANALYTICAL_TIME 22 min CH:WEAK_WASH_SOLVENT_NAME 10% methanol/90% water; 0.1% formic acid CH:STRONG_WASH_SOLVENT_NAME 75% 2-Propanol/25% Water; 0.1% formic acid CH:CHROMATOGRAPHY_TYPE Reversed phase #ANALYSIS AN:LABORATORY_NAME Sumner Lab, UNC-CH AN:ANALYSIS_TYPE MS AN:ACQUISITION_DATE 12/7/2022-12/12/2022 AN:SOFTWARE_VERSION Xcalibur 4.1.31.9 AN:DETECTOR_TYPE Orbitrap AN:DATA_FORMAT Profile #MS MS:INSTRUMENT_NAME Thermo Q Exactive HF-X Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:MS_COMMENTS Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data MS:MS_COMMENTS acquisition; Progenesis QI 2.4 for data preprocessing MS:ION_MODE POSITIVE MS:CAPILLARY_TEMPERATURE 320 °C MS:CAPILLARY_VOLTAGE 3.5 KV MS:COLLISION_ENERGY 20-45, ramp MS:COLLISION_GAS N2 MS:DRY_GAS_FLOW 55 MS:DRY_GAS_TEMP 400°C MS:FRAGMENTATION_METHOD CID MS:ION_SPRAY_VOLTAGE 3.5kV MS:IONIZATION ES+ MS:MASS_ACCURACY 5 ppm MS:DATAFORMAT Profile MS:DESOLVATION_GAS_FLOW 55 MS:DESOLVATION_TEMPERATURE 400 oC MS:MS_RESULTS_FILE ST002559_AN004218_Results.txt UNITS:m/z Has m/z:Yes Has RT:Yes RT units:Minutes #END